##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16219910
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.582765440745355	33.0	32.0	34.0	25.0	34.0
2	31.62848714943548	33.0	32.0	34.0	27.0	34.0
3	31.653820397277173	33.0	32.0	34.0	27.0	34.0
4	31.5170701317085	33.0	32.0	34.0	27.0	34.0
5	31.57576916271422	33.0	32.0	34.0	27.0	34.0
6	34.89253701161104	38.0	36.0	38.0	26.0	38.0
7	34.94962789559251	38.0	36.0	38.0	26.0	38.0
8	35.026047678439646	38.0	36.0	38.0	26.0	38.0
9	35.18301032496481	38.0	36.0	38.0	28.0	38.0
10	35.12967137302242	38.0	36.0	38.0	28.0	38.0
11	35.15804082760015	38.0	36.0	38.0	28.0	38.0
12	35.08757841443017	38.0	36.0	38.0	27.0	38.0
13	35.13419260649412	38.0	36.0	38.0	27.0	38.0
14	35.128150649417904	38.0	36.0	38.0	27.0	38.0
15	35.103041262251146	38.0	36.0	38.0	27.0	38.0
16	35.0878128793563	38.0	36.0	38.0	27.0	38.0
17	35.04560426044288	38.0	36.0	38.0	27.0	38.0
18	35.057133670902	38.0	36.0	38.0	27.0	38.0
19	35.061533448705944	38.0	36.0	38.0	27.0	38.0
20	35.03177711836872	38.0	36.0	38.0	26.0	38.0
21	35.01877969729795	38.0	36.0	38.0	26.0	38.0
22	35.04996710832551	38.0	36.0	38.0	27.0	38.0
23	35.04520043576074	38.0	36.0	38.0	27.0	38.0
24	35.04848386951592	38.0	36.0	38.0	27.0	38.0
25	35.0480004512972	38.0	36.0	38.0	27.0	38.0
26	35.03106484561258	38.0	36.0	38.0	27.0	38.0
27	35.03781463645606	38.0	36.0	38.0	27.0	38.0
28	35.05669322456166	38.0	36.0	38.0	27.0	38.0
29	35.05577694327527	38.0	36.0	38.0	27.0	38.0
30	35.05832368983552	38.0	36.0	38.0	27.0	38.0
31	35.087849254404	38.0	36.0	38.0	27.0	38.0
32	35.10838864087409	38.0	36.0	38.0	27.0	38.0
33	35.0803683867543	38.0	36.0	38.0	27.0	38.0
34	35.066522810545806	38.0	36.0	38.0	27.0	38.0
35	35.055774538822966	38.0	36.0	38.0	27.0	38.0
36	35.03818091469065	38.0	36.0	38.0	27.0	38.0
37	35.04019103681833	38.0	36.0	38.0	27.0	38.0
38	35.01431820521815	38.0	36.0	38.0	25.0	38.0
39	35.02427769327943	38.0	36.0	38.0	25.0	38.0
40	34.95169578622816	38.0	36.0	38.0	25.0	38.0
41	34.998852952944866	38.0	36.0	38.0	25.0	38.0
42	34.88748124989596	38.0	36.0	38.0	25.0	38.0
43	34.96944514488675	38.0	36.0	38.0	25.0	38.0
44	34.95920926811554	38.0	36.0	38.0	25.0	38.0
45	34.97526416607737	38.0	36.0	38.0	25.0	38.0
46	34.95005379191377	38.0	36.0	38.0	25.0	38.0
47	34.89703605013838	38.0	36.0	38.0	25.0	38.0
48	34.857679913143784	38.0	36.0	38.0	25.0	38.0
49	34.784663108488274	38.0	36.0	38.0	25.0	38.0
50	34.43731327732398	38.0	35.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	48.0
4	876.0
5	1908.0
6	2905.0
7	1433.0
8	600.0
9	422.0
10	352.0
11	352.0
12	374.0
13	343.0
14	412.0
15	477.0
16	998.0
17	3560.0
18	19078.0
19	59929.0
20	111230.0
21	149552.0
22	174226.0
23	192735.0
24	211560.0
25	232528.0
26	255837.0
27	281659.0
28	312119.0
29	350277.0
30	396769.0
31	450214.0
32	524181.0
33	626371.0
34	795488.0
35	1121810.0
36	2138337.0
37	7800950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27589592513825	18.586664541837386	13.32021267699133	24.81722685603303
2	15.045730833278359	20.812809688833045	38.887305786530256	25.25415369135834
3	18.057164861866546	26.41736746245074	29.83405440257501	25.691413273107706
4	12.686373183248728	17.254289504086262	36.96862179377703	33.090715518887976
5	13.716401174480017	36.944504382346246	34.696636754998515	14.642457688175222
6	31.109793031065113	36.0788033089019	18.584967722085604	14.226435937947384
7	26.936338466338665	31.34754082160204	23.446149400885492	18.269971311173798
8	26.884619910142444	32.94130814766542	21.08778520568041	19.086286736511727
9	26.96728488958689	14.400066645891249	20.458338604323963	38.1743098601979
10	16.188676667602746	28.02229906263053	32.46364915753125	23.325375112235477
11	32.90159612473718	23.26323853563558	23.547516863696707	20.28764847593053
12	23.39641670925555	25.32292739294148	30.53845984406638	20.74219605373659
13	29.40506419861153	21.04991015266828	25.824669931516624	23.720355717203564
14	23.19445071804088	20.414947187013976	27.30692893837666	29.08367315656848
15	25.048200322818303	26.74665204497146	24.71687381180474	23.488273820405496
16	23.132873033021887	26.580177539470352	26.71234381022841	23.57460561727935
17	22.651531673148053	26.754038669931063	27.487503265885156	23.106926391035728
18	23.445086308615217	25.220210799195343	29.343645828525933	21.991057063663508
19	23.366544828113856	26.105462743810794	29.11878271685987	21.40920971121548
20	24.102591250027146	24.821018010120575	29.061575135293083	22.014815604559193
21	23.678282701688847	25.2936726273625	28.36341072661207	22.664633944336583
22	23.534869679076884	25.208291771211982	27.62031979669692	23.63651875301422
23	22.560103961207524	25.641541494872982	27.99355029880395	23.804804245115545
24	22.647250704953777	26.608846639366675	27.709433204334445	23.034469451345114
25	23.54548933955613	24.87103973412371	27.543627731737967	24.03984319458219
26	23.611260322119296	25.078770556691754	28.01358739266295	23.296381728526004
27	23.428585001286322	25.04495344867262	27.525175843775585	24.001285706265474
28	22.886795659470597	25.107046589965186	28.40054865791944	23.605609092644784
29	23.174697585221946	24.667765683818637	28.830755800066722	23.326780930892696
30	22.98836237034394	25.689972154229224	28.6700211388006	22.65164433662624
31	24.444313261621154	24.562507340480863	27.572178516546803	23.42100088135118
32	22.279973654374736	25.455735580430684	28.5310944445757	23.733196320618884
33	23.305148522962295	25.681341964787574	27.89325286828232	23.12025664396781
34	22.607574897335926	25.143444711174865	28.168750050890978	24.08023034059822
35	22.60507626726395	26.211820795140273	28.889439841848674	22.293663095747103
36	24.039172745639334	24.994895205194247	27.86229083450286	23.10364121466356
37	23.564779816018568	25.594139736442358	28.140177504298812	22.700902943240266
38	23.853093208108266	25.443990491378727	27.739926645451657	22.96298965506135
39	23.70272473173913	24.802840707430747	27.850622914432982	23.643811646397136
40	23.36199739552241	25.76288023999943	27.839749042140276	23.035373322337886
41	22.814599317964397	25.5397433130613	27.802162045707025	23.84349532326728
42	24.000107099764296	25.863686183871295	27.760629065518778	22.37557765084564
43	24.201973255835114	24.614828937096338	27.258937469560184	23.924260337508365
44	23.393300381341113	25.73118968505731	27.753635278359763	23.121874655241818
45	24.543230563498597	24.915440928021326	27.340918698493205	23.200409809986873
46	22.88171140551649	25.28266897694606	28.756492034511883	23.079127583025564
47	24.153707479589407	25.577773712440617	27.64796958914054	22.62054921882943
48	24.174955268036907	25.639466512952865	27.22848791381421	22.957090305196022
49	23.329212623682977	25.92221235225899	28.27846867520418	22.47010634885385
50	23.695879601519383	25.9659657857523	26.878001687875262	23.460152924853052
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2366.0
1	2846.0
2	3326.0
3	9511.5
4	15697.0
5	17076.0
6	18455.0
7	15985.5
8	13516.0
9	17561.5
10	21607.0
11	30194.0
12	38781.0
13	70974.0
14	103167.0
15	122560.5
16	141954.0
17	130796.0
18	119638.0
19	104451.0
20	89264.0
21	100679.5
22	112095.0
23	130344.5
24	148594.0
25	181399.0
26	214204.0
27	240364.0
28	266524.0
29	308011.0
30	349498.0
31	390018.0
32	430538.0
33	466069.5
34	501601.0
35	555049.0
36	608497.0
37	651767.0
38	695037.0
39	737552.0
40	780067.0
41	810734.0
42	841401.0
43	870906.0
44	900411.0
45	965831.0
46	1031251.0
47	1148370.5
48	1265490.0
49	1319155.0
50	1372820.0
51	1231391.0
52	1089962.0
53	982465.0
54	874968.0
55	858579.0
56	842190.0
57	801291.5
58	760393.0
59	726474.5
60	692556.0
61	609230.0
62	525904.0
63	469413.0
64	412922.0
65	360049.0
66	307176.0
67	261158.0
68	215140.0
69	184429.5
70	153719.0
71	128393.5
72	103068.0
73	83321.0
74	63574.0
75	50540.5
76	37507.0
77	29196.5
78	20886.0
79	17242.0
80	13598.0
81	11030.0
82	8462.0
83	7233.0
84	6004.0
85	4649.5
86	3295.0
87	2919.5
88	2544.0
89	1359.5
90	175.0
91	116.0
92	57.0
93	31.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03251559348972959
2	0.0
3	9.864419716262298E-5
4	0.002688054372681476
5	0.024987808193756934
6	0.06873650963538022
7	0.05591276400423923
8	0.07831732728479998
9	0.0916157981147861
10	0.08648013460000703
11	0.07956887553630075
12	0.07786109787292284
13	0.06969212529539313
14	0.08894007426674994
15	0.07417427100396981
16	0.06647385836296256
17	0.06581417529443752
18	0.07557378555121452
19	0.07811387362815207
20	0.0748092930232042
21	0.06442082600951547
22	0.056942362812124114
23	0.06650468467457588
24	0.05498180939351698
25	0.06329258300446797
26	0.08815708595177162
27	0.06732466456349018
28	0.05823090263756088
29	0.05586960716798059
30	0.06108541909295428
31	0.05202248347863829
32	0.05702251122231874
33	0.054340622111959926
34	0.05416799476692534
35	0.059266666707768416
36	0.059556434036933614
37	0.05570314508526866
38	0.08103620796909478
39	0.04865625025046379
40	0.08141228897077728
41	0.07011136313333427
42	0.06611627314824804
43	0.059463955102093666
44	0.07951955343771945
45	0.010246665980267461
46	0.008514227267598895
47	0.009204736647737256
48	0.0170099587482298
49	0.05616553975946846
50	0.0043280141505100825
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.621991E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.27292660611651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.98279326707556	22.287135642480806
2	14.577830725370083	9.70094183603258
3	6.602481529493617	6.590516500472431
4	3.521074080009596	4.686257577554341
5	2.060875527554532	3.4285680086331793
6	1.3258340854282638	2.646862812980534
7	0.8832189429410822	2.0571095345927306
8	0.6503690553535407	1.7311745476534148
9	0.4933076744313018	1.477241104102805
>10	2.5174761836940194	15.086930245600898
>50	0.19853295444048016	4.5946216214073115
>100	0.1593185432874772	10.766141290195828
>500	0.017126855235070567	3.872768561535155
>1k	0.008682327968128171	4.880099565825857
>5k	6.692572037783674E-4	1.5586588668061572
>10k+	4.0899051342011347E-4	4.634972284125808
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	102465	0.6317236038917602	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	91277	0.5627466490257961	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80374	0.4955267939217912	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54619	0.33674046280158154	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42759	0.2636204516547872	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	40398	0.24906426731097767	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	38093	0.2348533376572373	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	36337	0.22402713701863944	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	33351	0.20561766372316492	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	32625	0.2011416832769109	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	24116	0.14868146617336347	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	23693	0.14607356021087664	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	22641	0.13958770424743416	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	22610	0.13939658111543157	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	16562	0.1021090745879601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.932209858131149E-5	0.0	0.0	0.0	0.0
2	3.7608100168250007E-4	0.0	0.0	0.0	0.0
3	5.73369396007746E-4	0.0	0.0	0.0	0.0
4	5.7953465833041E-4	0.0	0.0	3.699157393598362E-5	0.0
5	6.350220192343854E-4	6.165262322663936E-6	0.0	4.315683625864755E-5	0.0
6	6.350220192343854E-4	6.165262322663936E-6	0.0	4.315683625864755E-5	0.0
7	7.891535773009838E-4	6.165262322663936E-6	0.0	5.5487360903975426E-5	0.0
8	0.0014488366458260248	6.165262322663936E-6	0.0	6.78178855493033E-5	0.0
9	0.0014488366458260248	6.165262322663936E-6	0.0	8.63136725172951E-5	0.0
10	0.014395887523420291	6.165262322663936E-6	0.0	1.541315580665984E-4	0.0
11	0.014445209622001602	1.849578696799181E-5	0.0	1.726273450345902E-4	6.165262322663936E-6
12	0.01570908839814771	2.4661049290655745E-5	0.0	1.7879260735725414E-4	6.165262322663936E-6
13	0.01574607997208369	2.4661049290655745E-5	0.0	1.9728839432524596E-4	6.165262322663936E-6
14	0.01580773259531033	3.082631161331968E-5	0.0	2.219494436159017E-4	6.165262322663936E-6
15	0.015955698891054266	3.082631161331968E-5	0.0	2.589410175518853E-4	6.165262322663936E-6
16	0.015980359940344923	3.082631161331968E-5	0.0	2.8360206684254104E-4	6.165262322663936E-6
17	0.018150532277922626	3.082631161331968E-5	0.0	3.6991573935983617E-4	6.165262322663936E-6
18	0.018427969082442503	3.699157393598362E-5	0.0	3.945767886504919E-4	6.165262322663936E-6
19	0.01867457957534906	3.699157393598362E-5	0.0	4.1923783794114766E-4	6.165262322663936E-6
20	0.018995173216127585	3.699157393598362E-5	0.0	4.438988872318034E-4	6.165262322663936E-6
21	0.019808987842719224	3.699157393598362E-5	0.0	5.240472974264345E-4	6.165262322663936E-6
22	0.022379902231270087	3.699157393598362E-5	0.0	0.0013008703500820905	6.165262322663936E-6
23	0.025222088162018163	4.315683625864755E-5	0.0	0.002817524881457419	6.165262322663936E-6
24	0.027250459466174597	4.315683625864755E-5	0.0	0.003662165819662378	6.165262322663936E-6
25	0.027262789990819925	4.315683625864755E-5	0.0	0.003742314229857009	6.165262322663936E-6
26	0.027441582598177177	4.315683625864755E-5	0.0	0.003958098411150247	6.165262322663936E-6
27	0.027453913122822506	4.315683625864755E-5	0.0	0.0042170394287021325	6.165262322663936E-6
28	0.027515565746049145	4.932209858131149E-5	0.0	0.00518498561336037	6.165262322663936E-6
29	0.02758338363159845	4.932209858131149E-5	0.0	0.006935920112996928	6.165262322663936E-6
30	0.027589548893921113	4.932209858131149E-5	0.0	0.009728783945163692	6.165262322663936E-6
31	0.028101265666702218	4.932209858131149E-5	0.0	0.01344027186340738	6.165262322663936E-6
32	0.028403363520512752	4.932209858131149E-5	0.0	0.017046950322165782	6.165262322663936E-6
33	0.029426797066074966	4.932209858131149E-5	0.0	0.021504434981451808	6.165262322663936E-6
34	0.0303269253651839	4.932209858131149E-5	0.0	0.02704084054720402	6.165262322663936E-6
35	0.030536544284154473	4.932209858131149E-5	0.0	0.03464877425337132	6.165262322663936E-6
36	0.030616692694349107	4.932209858131149E-5	0.0	0.04286090366715968	6.165262322663936E-6
37	0.030746163203125048	4.932209858131149E-5	0.0	0.05506812306603427	6.165262322663936E-6
38	0.030776989514738368	5.5487360903975426E-5	0.0	0.06833576758440707	6.165262322663936E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACG	1625	0.0	17.59954	22
GTACTAG	2220	0.0	17.444485	1
AATGCGA	1800	0.0	16.132862	20
TCGACGA	1425	0.0	15.592335	37
CGACGAA	1425	0.0	15.438098	38
TAGGTCG	1755	0.0	15.418496	21
GTCGACG	1470	0.0	15.264813	36
CAATGCG	1875	0.0	15.134998	19
CGACGAG	2135	0.0	14.941247	24
CTAACGC	1285	0.0	14.552598	3
AGTCGAC	1575	0.0	14.526516	35
TCTAACG	1200	0.0	14.298348	2
TCTAGAC	2800	0.0	14.221497	3
CATTGCG	1880	0.0	14.040151	29
CGCGAAT	755	0.0	13.986304	35
GTCGTCA	2115	0.0	13.522273	24
CTAGACA	3285	0.0	13.394858	4
GTATTAG	2580	0.0	13.389934	1
CTAGTAC	2930	0.0	13.290166	3
ACGCGAA	795	0.0	13.282263	34
>>END_MODULE