FastQCFastQC Report
Wed 25 May 2016
SRR2558167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18285940
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2235731.2226497516671277No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1987491.086895177387654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1542580.8435880244603231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1034480.5657242668410811No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG898490.4913556535786511No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG869520.4755128803878827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT819820.44833352838300905No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC756570.41374411159612245No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG742710.40616451765673517No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA680450.3721165004369477No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC527330.28838003405895457No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT510590.2792254595607336No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC501790.27441301896429715No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC493520.2698904185401461No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA343990.1881172091781992No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT331590.18133604288322067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283540.1550590234901788No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG268120.1466263150814232No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT265400.14513883344252468No Hit
GTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCACTTTTTCGAACTG241860.13226555484705735No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC237190.1297116801214485No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC228050.1247133043201498No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTTGCAGACA225440.12328597818870672No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC201760.1103361380382961No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA199760.109242401539106No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG198520.10856428490960814No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC195320.106814306510904No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC193600.10587369312160054No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG189960.10388309269307457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189440.10359872120328514No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA189140.10343466072840664No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA185480.10143312293488878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG30150.022.40066124
TCTAGAC77100.021.1445583
CAATGCG31650.020.9903319
TGCGACG32250.020.80547122
AATGCGA33900.020.44153620
GTACTAG36500.020.2543351
CTAGACA84150.019.7398684
GTTCTAG85500.019.1460151
AGGTCGT31650.019.11465322
TAGACAT86050.019.0738415
CATTGCG31700.018.80371929
GACGAGT36850.018.74571425
ATCGTCA35350.018.73416910
CTCTAGT81050.018.67178327
CGTCATT32950.018.42737426
TCGACGA32150.018.26986537
GTCGTCA33750.017.99057824
CGAACAA34250.017.72881541
GCCTCTA64950.017.68076925
ATTAGGT34450.017.62405619
Produced by FastQC (version 0.11.5)