FastQCFastQC Report
Wed 25 May 2016
SRR2558166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19893304
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT962190.4836753110493862No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA913960.4594309723512997No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT807360.40584510245256394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT644110.3237823138881304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512710.2577299376714899No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG427230.21476070541122783No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG357630.17977405864807575No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG337000.16940373504572193No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG286800.14416911338609212No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCACAGCCAT267960.13469859003813545No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC263660.1325370587007568No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCAGCTATGG262790.13209972561621738No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA234500.11787886014309136No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC211920.10652830721332163No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC207980.10454774129023514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAT71200.018.5711025
TCTAGAC66700.018.3059753
CTCTAGT63650.018.14340827
CTAGACA72500.017.9042954
GTTCTAG76050.017.6479191
AATGCGA14400.017.56863820
CCGGCTC54200.017.53314833
TGCGACG13750.017.43937722
CTCTACC176200.017.3568841
CCGAACA18150.016.84792321
GAATCTA119600.016.61177813
CGTGAAT55950.016.55555710
CCTTACC181000.016.53953728
CGACGAG14800.016.49936924
CAATGCG14800.016.49878719
CGGCTCC58200.016.4805534
TCCGAAC18350.016.42431420
AATCTAC120450.016.38462314
CTTACCT185950.016.25294729
ACGCCGT56850.016.25267624
Produced by FastQC (version 0.11.5)