##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19893304
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.545046715216337	33.0	32.0	34.0	25.0	34.0
2	31.591014091977883	33.0	32.0	34.0	27.0	34.0
3	31.61707426780388	33.0	32.0	34.0	27.0	34.0
4	31.47817934114916	33.0	32.0	34.0	27.0	34.0
5	31.52653802505607	33.0	32.0	34.0	27.0	34.0
6	34.86107410815217	38.0	36.0	38.0	26.0	38.0
7	34.91242480384354	38.0	36.0	38.0	26.0	38.0
8	34.993407982907215	38.0	36.0	38.0	26.0	38.0
9	35.16642278225879	38.0	36.0	38.0	28.0	38.0
10	35.10669479539447	38.0	36.0	38.0	27.0	38.0
11	35.14231090019034	38.0	36.0	38.0	28.0	38.0
12	35.070155163767666	38.0	36.0	38.0	27.0	38.0
13	35.11199678042421	38.0	36.0	38.0	27.0	38.0
14	35.10473981597024	38.0	36.0	38.0	27.0	38.0
15	35.07813282298405	38.0	36.0	38.0	27.0	38.0
16	35.067490096165024	38.0	36.0	38.0	27.0	38.0
17	35.02794910287401	38.0	36.0	38.0	27.0	38.0
18	35.04589614676375	38.0	36.0	38.0	27.0	38.0
19	35.04474017991179	38.0	36.0	38.0	27.0	38.0
20	35.01486585637056	38.0	36.0	38.0	26.0	38.0
21	35.019202491451395	38.0	36.0	38.0	26.0	38.0
22	35.041796023425775	38.0	36.0	38.0	27.0	38.0
23	35.042955659854194	38.0	36.0	38.0	27.0	38.0
24	35.047126158631066	38.0	36.0	38.0	27.0	38.0
25	35.04210698232933	38.0	36.0	38.0	27.0	38.0
26	35.0134147650888	38.0	36.0	38.0	27.0	38.0
27	35.03155458741293	38.0	36.0	38.0	27.0	38.0
28	35.05250063036286	38.0	36.0	38.0	27.0	38.0
29	35.04673250858681	38.0	36.0	38.0	27.0	38.0
30	35.0616859823788	38.0	36.0	38.0	27.0	38.0
31	35.08779190224007	38.0	36.0	38.0	27.0	38.0
32	35.11761892343273	38.0	36.0	38.0	27.0	38.0
33	35.08068845677923	38.0	36.0	38.0	27.0	38.0
34	35.06446229344306	38.0	36.0	38.0	27.0	38.0
35	35.06763642680974	38.0	36.0	38.0	27.0	38.0
36	35.059094105232596	38.0	36.0	38.0	27.0	38.0
37	35.06064352105613	38.0	36.0	38.0	27.0	38.0
38	35.033338303179804	38.0	36.0	38.0	27.0	38.0
39	35.04363513471669	38.0	36.0	38.0	27.0	38.0
40	34.9656720170767	38.0	36.0	38.0	25.0	38.0
41	35.018711471960614	38.0	36.0	38.0	26.0	38.0
42	34.905307383831264	38.0	36.0	38.0	25.0	38.0
43	34.982489384367724	38.0	36.0	38.0	25.0	38.0
44	34.976081851461174	38.0	36.0	38.0	25.0	38.0
45	34.99650726696782	38.0	36.0	38.0	25.0	38.0
46	34.97338596946993	38.0	36.0	38.0	25.0	38.0
47	34.922952265747305	38.0	36.0	38.0	25.0	38.0
48	34.883031647231654	38.0	36.0	38.0	25.0	38.0
49	34.818113170140066	38.0	36.0	38.0	25.0	38.0
50	34.466000770912665	38.0	35.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	48.0
4	1107.0
5	2275.0
6	3598.0
7	1820.0
8	677.0
9	512.0
10	464.0
11	450.0
12	431.0
13	445.0
14	471.0
15	585.0
16	1204.0
17	4367.0
18	23881.0
19	73902.0
20	134702.0
21	179544.0
22	210830.0
23	235564.0
24	259394.0
25	285367.0
26	314404.0
27	346754.0
28	385388.0
29	432544.0
30	489576.0
31	556708.0
32	645295.0
33	773481.0
34	983036.0
35	1386442.0
36	2646778.0
37	9511260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91216326331938	17.772662975165463	12.738203427063016	25.57697033445214
2	16.023265919024812	20.240931320408112	38.57769428346342	25.158108477103653
3	18.541772041435898	24.937720181114408	29.795706912514486	26.724800864935204
4	13.468424204256085	16.39828356264206	35.851431309528984	34.28186092357287
5	14.69732560102239	36.1307660039041	33.867623503022045	15.30428489205146
6	32.70123093818468	34.731372494969264	17.842431582295916	14.724964984550143
7	28.59918841246826	29.957398721182045	22.57451828808053	18.86889457826917
8	27.122402371329656	32.473252941108704	20.70026083881646	19.704083848745178
9	27.677320002537837	13.589721412879568	19.992475037839903	38.74048354674269
10	16.547668843768204	27.508606492343585	31.331093543991194	24.612631119897017
11	33.915850809494856	22.765214747278048	22.937118015794574	20.381816427432522
12	24.45765430493907	24.388632971108837	29.89165059187303	21.262062132079063
13	30.459418110501517	20.424358017185675	25.1031988499557	24.01302502235711
14	24.20528229633873	19.572619273187918	26.508162691740665	29.713935738732683
15	25.36473695560979	26.706735133453037	23.844260782213432	24.084267128723738
16	24.61982785935249	25.141725618719263	25.54107896328089	24.69736755864735
17	22.78091893320711	26.556418692139744	26.7605299407681	23.902132433885043
18	24.424997633093348	24.189096775342477	28.53935549509198	22.8465500964722
19	24.36956835722014	24.859862855382392	28.257997130373614	22.512571657023855
20	25.303890760554804	23.986056970692164	28.090957278847554	22.61909498990548
21	24.508904036481113	23.95893892991003	28.218578032159495	23.31357900144936
22	24.22792931974408	24.050975184171726	26.934917172266676	24.786178323817516
23	23.366240752003623	24.561092874855817	27.137320167683114	24.935346205457442
24	23.342225815808273	25.41289789022286	27.215688980626375	24.02918731334249
25	25.191146101992707	24.103565587489598	26.62257708278894	24.082711227728755
26	24.964515891914584	24.285433757908297	26.902812812707555	23.847237537469564
27	24.555114261937263	23.833748760559388	26.97835143936077	24.632785538142578
28	23.2979058201773	24.165547847060456	27.64705559859041	24.889490734171833
29	23.967094991143085	23.783262225344163	27.588741306860342	24.66090147665241
30	24.123807084549142	24.480477059546534	28.12273591774791	23.272979938156414
31	25.444459560762272	23.75267535773912	27.06999070304541	23.732874378453197
32	23.51475406603872	24.404838098570146	27.837314491848208	24.24309334354293
33	24.419081174478993	24.390508139316506	26.819744233864935	24.370666452339567
34	23.528109410160404	23.797763072637444	27.046432344839626	25.627695172362525
35	23.44664423347613	24.90435732057752	28.140593443580592	23.508405002365762
36	24.828596712698463	23.675558462114026	27.7301119625235	23.765732862664006
37	24.846933930663027	24.321078323877	27.530476408521686	23.301511336938287
38	24.802249279554996	23.87553881820356	27.77954528829824	23.542666613943204
39	25.01197342997441	23.491117734210125	27.32766471116989	24.16924412464558
40	24.59315889448511	24.6814463813626	26.678291093538803	24.047103630613485
41	24.58533797348958	24.0324418573241	27.344244974333616	24.03797519485271
42	25.045518983819402	24.282738671159052	26.547930910238332	24.123811434783217
43	24.679013282091077	24.0698592883397	26.2634353490691	24.987692080500132
44	24.555679832004937	24.528513748075927	26.7160224104094	24.199784009509738
45	24.914416028367803	23.907256258935135	26.490030834109596	24.688296878587472
46	23.931535030862815	24.3934894242155	27.65236174874849	24.022613796173196
47	24.991794212679025	24.38306027881629	26.725519091143134	23.89962641736155
48	24.76596653081446	24.642723676630016	26.157233708984474	24.434076083571053
49	23.860371194122553	24.874983484114296	27.668895458303695	23.595749863459456
50	24.79172950473548	24.4689982103718	26.53550099535501	24.203771289537713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2755.0
1	3410.5
2	4066.0
3	10953.5
4	17841.0
5	19512.5
6	21184.0
7	18465.5
8	15747.0
9	20417.5
10	25088.0
11	34796.0
12	44504.0
13	83125.5
14	121747.0
15	143985.5
16	166224.0
17	151073.0
18	135922.0
19	112560.0
20	89198.0
21	91806.0
22	94414.0
23	104147.0
24	113880.0
25	144343.0
26	174806.0
27	193199.5
28	211593.0
29	254705.0
30	297817.0
31	332504.5
32	367192.0
33	404805.0
34	442418.0
35	521586.0
36	600754.0
37	644801.5
38	688849.0
39	781466.0
40	874083.0
41	915268.0
42	956453.0
43	1028594.5
44	1100736.0
45	1172429.0
46	1244122.0
47	1389289.5
48	1534457.0
49	1649063.0
50	1763669.0
51	1625339.5
52	1487010.0
53	1358978.5
54	1230947.0
55	1213162.5
56	1195378.0
57	1152585.0
58	1109792.0
59	1054167.5
60	998543.0
61	888263.0
62	777983.0
63	698060.0
64	618137.0
65	544991.0
66	471845.0
67	391408.5
68	310972.0
69	270315.5
70	229659.0
71	200298.0
72	170937.0
73	124629.5
74	78322.0
75	63675.0
76	49028.0
77	33989.5
78	18951.0
79	15549.0
80	12147.0
81	10607.0
82	9067.0
83	8111.5
84	7156.0
85	5753.5
86	4351.0
87	3842.5
88	3334.0
89	1732.5
90	131.0
91	101.0
92	71.0
93	44.0
94	17.0
95	10.0
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03198563697614031
2	0.0
3	1.10589975400768E-4
4	0.002372657654052841
5	0.0250586830624013
6	0.06790224489607156
7	0.055480979931739845
8	0.07797095947460513
9	0.09109095201078715
10	0.0856066945943218
11	0.079654943190935
12	0.07737276824402825
13	0.06897798374769722
14	0.08862278483252455
15	0.07370821860461188
16	0.06617804664323232
17	0.06519279049875275
18	0.07559327500348861
19	0.07768945771903953
20	0.07387913038477671
21	0.06419245390308216
22	0.05688346189250412
23	0.06628863661863309
24	0.05512910273728286
25	0.06318206367328424
26	0.08799443269956565
27	0.0671984905071576
28	0.057743047610391916
29	0.05581274985794215
30	0.06122663183551611
31	0.05252521149830113
32	0.05710966866036934
33	0.054902895969417644
34	0.05410363205629392
35	0.05907012731520113
36	0.05946724586323117
37	0.055496060382930854
38	0.0804592339211224
39	0.048734991432293
40	0.08140930234615627
41	0.06937510229572724
42	0.06603729576544952
43	0.059296334083066345
44	0.07830272940080742
45	0.010159197285679643
46	0.008696393520151304
47	0.009324745653110212
48	0.016623684029560902
49	0.05494813732299069
50	0.004544242625558831
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.9893304E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.921128814222236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.74256895992936	16.9224210473947
2	13.133942549667433	6.2835746313837815
3	5.657946677323919	4.060334138767984
4	2.947050304958556	2.819870798676262
5	1.7048048474297355	2.0390428179238596
6	1.0976151766067648	1.5753716416833425
7	0.7685205265807943	1.286871495889916
8	0.526383572585044	1.0073351396397872
9	0.39862453468733117	0.8581993958488543
>10	2.3697939141987248	10.847102954401363
>50	0.2814904458979842	4.734642312036193
>100	0.2831813240624778	14.335893307238774
>500	0.04841890642417802	8.13566003080219
>1k	0.036685535070333876	16.72345508643177
>5k	0.0021926479153214743	3.44110317398489
>10k+	7.800766621816784E-4	4.929122027896496
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96219	0.4836753110493862	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	91396	0.4594309723512997	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	80736	0.40584510245256394	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64411	0.3237823138881304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51271	0.2577299376714899	No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG	42723	0.21476070541122783	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	35763	0.17977405864807575	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	33700	0.16940373504572193	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	28680	0.14416911338609212	No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCACAGCCAT	26796	0.13469859003813545	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	26366	0.1325370587007568	No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCAGCTATGG	26279	0.13209972561621738	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	23450	0.11787886014309136	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	21192	0.10652830721332163	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	20798	0.10454774129023514	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.0268170636712734E-5	0.0	0.0	0.0	0.0
2	3.418235603296466E-4	5.026817063671273E-6	0.0	0.0	0.0
3	4.5744035279408587E-4	5.026817063671273E-6	0.0	0.0	0.0
4	4.624671698577572E-4	5.026817063671273E-6	0.0	5.026817063671273E-6	0.0
5	5.077085234307986E-4	5.026817063671273E-6	0.0	5.026817063671273E-6	0.0
6	5.077085234307986E-4	5.026817063671273E-6	0.0	5.026817063671273E-6	0.0
7	6.585130353409368E-4	5.026817063671273E-6	0.0	2.0107268254685093E-5	0.0
8	0.0012064360952811057	5.026817063671273E-6	0.0	2.0107268254685093E-5	0.0
9	0.001211462912344777	5.026817063671273E-6	0.0	5.0268170636712734E-5	0.0
10	0.012049280501620043	1.508045119101382E-5	0.0	1.0053634127342547E-4	0.0
11	0.012089495038129413	1.508045119101382E-5	0.0	1.156167924644393E-4	0.0
12	0.013180314340946079	1.508045119101382E-5	0.0	1.3572406071912438E-4	0.0
13	0.013195394792137093	1.508045119101382E-5	0.0	1.508045119101382E-4	0.0
14	0.013205448426264435	1.508045119101382E-5	0.0	1.6085814603748074E-4	0.0
15	0.013346199304047231	1.508045119101382E-5	0.0	1.8096541429216584E-4	0.0
16	0.013361279755238245	2.0107268254685093E-5	0.0	2.0107268254685094E-4	0.0
17	0.0153116847759427	2.0107268254685093E-5	0.0	2.613944873109062E-4	0.0
18	0.015527837909680564	2.0107268254685093E-5	0.0	3.1166265794761895E-4	0.0
19	0.015723883775163743	2.0107268254685093E-5	0.0	3.317699262023041E-4	0.0
20	0.015995331896601992	2.0107268254685093E-5	0.0	4.0214536509370187E-4	0.0
21	0.016819729895044083	2.0107268254685093E-5	0.0	5.077085234307986E-4	0.0
22	0.019373352963389087	2.0107268254685093E-5	0.0	0.0011461142905170505	0.0
23	0.022087834177771576	3.016090238202764E-5	0.0	0.0025335158000903218	0.0
24	0.024013105113157674	3.016090238202764E-5	0.0	0.0033378065302777254	0.0
25	0.024013105113157674	3.016090238202764E-5	0.0	0.003418235603296466	0.0
26	0.02415385599094047	3.016090238202764E-5	0.0	0.003654496005289016	0.0
27	0.02415385599094047	3.016090238202764E-5	0.0	0.003956105029109292	0.0
28	0.024189043710386167	6.032180476405528E-5	0.0	0.0050971925025626716	0.0
29	0.024259419149277567	6.534862182772656E-5	0.0	0.006836471206592932	0.0
30	0.02426444596634124	6.534862182772656E-5	0.0	0.009495657433275035	0.0
31	0.02478723494096305	6.534862182772656E-5	0.0	0.01286362486593479	0.0
32	0.02501846852589193	6.534862182772656E-5	0.0	0.016794595809725726	0.0
33	0.025802651987824648	7.037543889139782E-5	0.0	0.020971880789636552	0.0
34	0.026546620913247995	7.54022559550691E-5	0.0	0.02597859058505314	0.0
35	0.026697425425158135	7.54022559550691E-5	0.0	0.03337303848571359	0.0
36	0.02676277404698586	7.54022559550691E-5	0.0	0.04227553150547541	0.0
37	0.026818069034686243	7.54022559550691E-5	0.0	0.05487273506703562	0.0
38	0.02688341765651397	7.54022559550691E-5	0.0	0.06792235216432625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACAT	7120	0.0	18.571102	5
TCTAGAC	6670	0.0	18.305975	3
CTCTAGT	6365	0.0	18.143408	27
CTAGACA	7250	0.0	17.904295	4
GTTCTAG	7605	0.0	17.647919	1
AATGCGA	1440	0.0	17.568638	20
CCGGCTC	5420	0.0	17.533148	33
TGCGACG	1375	0.0	17.439377	22
CTCTACC	17620	0.0	17.356884	1
CCGAACA	1815	0.0	16.847923	21
GAATCTA	11960	0.0	16.611778	13
CGTGAAT	5595	0.0	16.555557	10
CCTTACC	18100	0.0	16.539537	28
CGACGAG	1480	0.0	16.499369	24
CAATGCG	1480	0.0	16.498787	19
CGGCTCC	5820	0.0	16.48055	34
TCCGAAC	1835	0.0	16.424314	20
AATCTAC	12045	0.0	16.384623	14
CTTACCT	18595	0.0	16.252947	29
ACGCCGT	5685	0.0	16.252676	24
>>END_MODULE