##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558164_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30904806
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635707727788358	33.0	32.0	34.0	27.0	34.0
2	31.678995364022022	33.0	32.0	34.0	27.0	34.0
3	31.70154680796249	33.0	32.0	34.0	27.0	34.0
4	31.556450119764545	33.0	32.0	34.0	27.0	34.0
5	31.61838838270009	33.0	32.0	34.0	27.0	34.0
6	34.91142351775319	38.0	36.0	38.0	26.0	38.0
7	34.97791641209461	38.0	36.0	38.0	26.0	38.0
8	35.05248678797725	38.0	36.0	38.0	26.0	38.0
9	35.198618719690394	38.0	36.0	38.0	28.0	38.0
10	35.120538274856024	38.0	36.0	38.0	27.0	38.0
11	35.14687236671215	38.0	36.0	38.0	28.0	38.0
12	35.081202062876564	38.0	36.0	38.0	27.0	38.0
13	35.12846765645447	38.0	36.0	38.0	27.0	38.0
14	35.145324452125664	38.0	36.0	38.0	27.0	38.0
15	35.13097568061097	38.0	36.0	38.0	27.0	38.0
16	35.12972603678535	38.0	36.0	38.0	27.0	38.0
17	35.06962260174033	38.0	36.0	38.0	27.0	38.0
18	35.07628040117773	38.0	36.0	38.0	27.0	38.0
19	35.09555452313792	38.0	36.0	38.0	27.0	38.0
20	35.05688335335287	38.0	36.0	38.0	27.0	38.0
21	35.03490573602048	38.0	36.0	38.0	26.0	38.0
22	35.05475070770546	38.0	36.0	38.0	27.0	38.0
23	35.055050143333695	38.0	36.0	38.0	27.0	38.0
24	35.055741750975564	38.0	36.0	38.0	27.0	38.0
25	35.06734214736698	38.0	36.0	38.0	27.0	38.0
26	35.04630655180298	38.0	36.0	38.0	27.0	38.0
27	35.04250581608569	38.0	36.0	38.0	27.0	38.0
28	35.06487751451991	38.0	36.0	38.0	27.0	38.0
29	35.06076805659288	38.0	36.0	38.0	27.0	38.0
30	35.04882936330356	38.0	36.0	38.0	27.0	38.0
31	35.08015332631436	38.0	36.0	38.0	27.0	38.0
32	35.096692922129975	38.0	36.0	38.0	27.0	38.0
33	35.05879425355396	38.0	36.0	38.0	27.0	38.0
34	35.0362732903096	38.0	36.0	38.0	27.0	38.0
35	35.01155066302633	38.0	36.0	38.0	25.0	38.0
36	34.98788880926805	38.0	36.0	38.0	25.0	38.0
37	34.97881462190703	38.0	36.0	38.0	25.0	38.0
38	34.937655165995864	38.0	36.0	38.0	25.0	38.0
39	34.94121966013959	38.0	36.0	38.0	25.0	38.0
40	34.83828049268453	38.0	36.0	38.0	25.0	38.0
41	34.87730934146618	38.0	36.0	38.0	25.0	38.0
42	34.75150470124291	38.0	36.0	38.0	25.0	38.0
43	34.82126543683853	38.0	36.0	38.0	25.0	38.0
44	34.809687172927084	38.0	36.0	38.0	25.0	38.0
45	34.80333405102106	38.0	36.0	38.0	25.0	38.0
46	34.759690127160155	38.0	36.0	38.0	25.0	38.0
47	34.67028759216285	38.0	36.0	38.0	25.0	38.0
48	34.60874690493122	38.0	36.0	38.0	24.0	38.0
49	34.53206478629893	38.0	36.0	38.0	24.0	38.0
50	34.17332313944957	38.0	35.0	38.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	87.0
4	1815.0
5	3925.0
6	5865.0
7	3032.0
8	1052.0
9	807.0
10	752.0
11	709.0
12	701.0
13	695.0
14	730.0
15	995.0
16	2257.0
17	8185.0
18	44691.0
19	134188.0
20	237249.0
21	303540.0
22	340028.0
23	368066.0
24	400744.0
25	437721.0
26	479613.0
27	529231.0
28	592315.0
29	669826.0
30	759257.0
31	858177.0
32	994760.0
33	1191133.0
34	1509708.0
35	2123587.0
36	4053845.0
37	1.484552E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.286934278510856	18.153989429158234	13.593460853407159	23.96561543892376
2	13.813916191546388	21.58608276007298	38.73403379396719	25.86596725441344
3	18.411470646868942	28.523789586983057	29.13912519821784	23.925614567930165
4	12.386325188784966	18.2382740533053	35.80849421678993	33.5669065411198
5	12.813681256153323	38.08721895952831	34.375005360565126	14.724094423753249
6	30.546695802499773	34.85672910423374	19.357696578473824	15.238878514792662
7	25.52674643116941	32.2626863154972	23.93296728016704	18.277599973166346
8	28.11554830440248	31.929174096112035	21.324152233626453	18.63112536585903
9	28.0002061193746	14.286456441314666	20.122714873294235	37.5906225660165
10	16.716537373979587	28.83674344556534	31.880726925353088	22.56599225510199
11	31.6263548412388	24.32363794835627	22.07929942779535	21.970707782609576
12	23.736674050181996	26.499086775735435	30.332212852498092	19.432026321584477
13	30.047031528972845	21.19947366549769	24.966082471549527	23.78741233397994
14	23.48907303229046	21.26171186083977	27.05083187433367	28.198383232536102
15	26.923229992544584	24.9295705458853	25.48861187696564	22.65858758460448
16	21.644317006457467	26.512340751041446	28.92837195561116	22.914970286889925
17	23.108590688396653	26.527477491887907	28.066213411082646	22.297718408632793
18	24.352141233847348	23.88924745978043	30.68515875637619	21.073452549996027
19	22.748303700142916	27.09013313789016	30.59100510616018	19.570558055806746
20	24.075220899923146	24.143907756572823	30.73692308917867	21.043948254325368
21	22.734227969695343	25.762670067539865	29.42897340495268	22.07412855781211
22	22.918020698071842	25.44817643577729	27.319548476110434	24.314254390040432
23	20.996393978813046	26.621111585512786	28.502012055299968	23.8804823803742
24	21.63598168338718	28.730329528407278	27.646520518611318	21.987168269594225
25	22.936950602070986	24.34181365981624	27.351470814364998	25.369764923747777
26	23.332307415571734	24.807727865073993	28.79850153607515	23.061463183279123
27	23.5061070757345	24.648361403092256	27.22282201861741	24.622709502555832
28	22.393498746044184	24.969966026845235	29.319409851570576	23.31712537554001
29	23.180971710699147	23.567030119743507	30.566417360480713	22.685580809076633
30	22.589558430583402	26.086432694479228	30.284368729201894	21.039640145735472
31	25.596237301303066	23.59460998255029	27.446791138118844	23.362361578027805
32	20.36414499984103	25.521762855710318	30.259629572574227	23.85446257187443
33	22.71844831190566	26.368115133751242	28.459407537732222	22.45402901661088
34	20.713193994687483	25.2980536957687	29.51452602210143	24.474226287442388
35	21.05744444369616	27.39703039207103	30.473526929725814	21.071998234506996
36	24.001596489820486	25.008413399039764	28.503909535092763	22.486080576046994
37	22.974904011803172	26.318919428192345	28.94395338303556	21.76222317696892
38	24.12042208275773	25.671662005022316	27.83704592201412	22.370869990205836
39	23.95741819116665	23.778269526264005	28.30938042377787	23.954931858791472
40	22.808146076658197	26.24919069829621	28.432248849619242	22.51041437542635
41	21.545932011121565	26.24443721117472	27.82941592627833	24.38021485142539
42	24.46615181099327	26.627037376739693	28.394757955599058	20.51205285666798
43	24.598264401715138	23.771714405160118	27.17044652168034	24.4595746714444
44	23.016343642905927	25.994011289383067	28.64698684071146	22.34265822699955
45	26.293609030220793	23.731612989242944	27.678260360234123	22.29651762030214
46	21.46487738323155	25.033565729967332	31.332457706533035	22.16909918026808
47	24.74317700558038	25.453753091888288	28.59293631431772	21.210133588213615
48	24.459791145715982	25.74802169541077	28.037014783246217	21.755172375627026
49	23.01295202123504	26.100269420301984	30.200236768691752	20.686541789771223
50	23.535192587403902	26.28232100179796	27.064147672827037	23.118338737971104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5889.0
1	6104.5
2	6320.0
3	21830.5
4	37341.0
5	42655.5
6	47970.0
7	42800.5
8	37631.0
9	48869.0
10	60107.0
11	82825.0
12	105543.0
13	188048.0
14	270553.0
15	323444.5
16	376336.0
17	347350.5
18	318365.0
19	268604.0
20	218843.0
21	229719.5
22	240596.0
23	275523.0
24	310450.0
25	364994.0
26	419538.0
27	451923.5
28	484309.0
29	557368.5
30	630428.0
31	691169.0
32	751910.0
33	808596.0
34	865282.0
35	947112.0
36	1028942.0
37	1090039.5
38	1151137.0
39	1213133.0
40	1275129.0
41	1342575.0
42	1410021.0
43	1482161.0
44	1554301.0
45	1786163.5
46	2018026.0
47	2546834.0
48	3075642.0
49	3327104.0
50	3578566.0
51	2987506.0
52	2396446.0
53	1936421.0
54	1476396.0
55	1457533.5
56	1438671.0
57	1367526.0
58	1296381.0
59	1246486.0
60	1196591.0
61	1005634.5
62	814678.0
63	712281.5
64	609885.0
65	523821.0
66	437757.0
67	376755.5
68	315754.0
69	268851.5
70	221949.0
71	183778.0
72	145607.0
73	125665.5
74	105724.0
75	84119.0
76	62514.0
77	51341.5
78	40169.0
79	32695.5
80	25222.0
81	21068.5
82	16915.0
83	15107.0
84	13299.0
85	10015.5
86	6732.0
87	5601.5
88	4471.0
89	2384.0
90	297.0
91	225.5
92	154.0
93	82.5
94	11.0
95	6.5
96	2.0
97	3.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032389784294390975
2	0.0
3	1.4560842090385553E-4
4	0.0027892101959805216
5	0.02548794514354822
6	0.07225413419517987
7	0.05894876026725423
8	0.08227199355336513
9	0.09543499480307367
10	0.09048107274965582
11	0.08354364042925881
12	0.08156013016227961
13	0.07294658313014486
14	0.09263284163634614
15	0.07735042892681482
16	0.0700117645132605
17	0.06883718991796939
18	0.07928540305349271
19	0.0816766168990027
20	0.07791668389699648
21	0.06786323136925694
22	0.06032718665181072
23	0.06943903805770534
24	0.058537820946036684
25	0.06684073668024319
26	0.09185302764883882
27	0.07152285634797384
28	0.06131408817127018
29	0.05917526225532689
30	0.06497694889267384
31	0.055615945299899314
32	0.060340129622557734
33	0.05784537201107168
34	0.05744737566060114
35	0.06271516475463396
36	0.06325229804063486
37	0.058877573928145674
38	0.08483146601858624
39	0.051561559713398625
40	0.08521004791293625
41	0.073820233655568
42	0.06985321312160964
43	0.0625080772226818
44	0.08317476576296903
45	0.01047086333433059
46	0.008904763873942454
47	0.009636041721148484
48	0.01736946674248659
49	0.05863489322663924
50	0.004559161445634054
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.0904806E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.974797534771913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.527725569826146	13.960089072414819
2	12.067723632525569	5.303715670606633
3	6.67122068209364	4.397961713963721
4	4.3334204782305585	3.8090415056860394
5	2.7763153809236414	3.0504484194235086
6	2.0291238228186517	2.675375110765336
7	1.484406615221779	2.283367438014227
8	1.1080821086204162	1.9479903991069427
9	0.8522565937588334	1.685534948597225
>10	4.577088615634772	17.88532520615029
>50	0.30683073642280695	4.672947501109339
>100	0.22094459257394156	9.572728591638066
>500	0.024985479783093102	3.797254144229311
>1k	0.017389587090313498	7.290610493618386
>5k	0.0011246663105348056	1.6837407183473299
>10k+	0.0013614381653803552	15.983869066328754
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	535508	1.7327660946973749	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	459532	1.4869273083286787	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	216326	0.6999752724543878	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	215981	0.6988589412274583	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	206797	0.6691418803923248	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	177797	0.5753053424765067	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	176237	0.5702575838851731	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	158027	0.5113347095594128	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143018	0.46276944757394695	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	120005	0.3883053011237152	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	119480	0.3866065362131702	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	118038	0.3819405952588733	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	114737	0.37125940864990387	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114043	0.3690138032252977	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	89863	0.29077354505962605	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	79531	0.25734185162010076	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	64355	0.20823622060594718	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	62616	0.20260926407368482	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	57193	0.18506183148342686	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	56476	0.18274180397702544	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	49183	0.1591435325625406	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	47620	0.15408606674314668	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	47276	0.15297297125890388	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	46408	0.15016434660680283	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	45729	0.14796727732249798	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	44234	0.14312984200580323	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	43327	0.14019502338891887	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	41855	0.1354320101540194	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	41115	0.13303756056582267	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40034	0.1295397227214434	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	39998	0.1294232359847203	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	38437	0.12437224165069989	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	38010	0.12299057952345664	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	36374	0.11769690448792981	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	36295	0.11744128081567637	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	34883	0.11287241214198206	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	32447	0.10499014295705335	No Hit
GTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCACTTTTTCGAACTG	31521	0.10199384522912068	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	31144	0.10077397023621504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.500762567478987E-5	0.0	0.0	0.0	0.0
2	4.4653249077182365E-4	0.0	0.0	9.707228060257035E-6	0.0
3	7.798139875073153E-4	0.0	0.0	9.707228060257035E-6	0.0
4	7.927569582543246E-4	6.47148537350469E-6	0.0	1.6178713433761727E-5	0.0
5	9.09243694977409E-4	9.707228060257035E-6	0.0	1.941445612051407E-5	0.0
6	9.09243694977409E-4	9.707228060257035E-6	0.0	1.941445612051407E-5	0.0
7	0.0011292741976765685	9.707228060257035E-6	0.0	2.2650198807266417E-5	0.0
8	0.0021355901732565477	9.707228060257035E-6	0.0	2.9121684180771107E-5	6.47148537350469E-6
9	0.00215176888669031	9.707228060257035E-6	0.0	4.5300397614532835E-5	6.47148537350469E-6
10	0.02284757911115831	9.707228060257035E-6	0.0	1.0354376597607504E-4	9.707228060257035E-6
11	0.023002894760122423	9.707228060257035E-6	0.0	1.2942970747009382E-4	9.707228060257035E-6
12	0.025659439505946097	2.2650198807266417E-5	0.0	1.714943623978743E-4	9.707228060257035E-6
13	0.025720918616994392	2.2650198807266417E-5	0.0	1.8767307583163602E-4	1.6178713433761727E-5
14	0.02581475515491021	2.2650198807266417E-5	0.0	2.1032327463890244E-4	1.6178713433761727E-5
15	0.026138329423585446	2.2650198807266417E-5	0.0	2.2326624538591183E-4	2.588594149401876E-5
16	0.02619657279194699	2.2650198807266417E-5	0.0	2.847453564342064E-4	3.55931695542758E-5
17	0.029509973303181388	2.2650198807266417E-5	0.0	3.7534615166327204E-4	3.55931695542758E-5
18	0.030017984905001505	2.9121684180771107E-5	0.0	4.0446783584404316E-4	3.882891224102814E-5
19	0.030425688483532304	2.9121684180771107E-5	0.0	4.5300397614532836E-4	3.882891224102814E-5
20	0.030865749488930623	2.9121684180771107E-5	0.0	5.177188298803753E-4	4.853614030128518E-5
21	0.03231859795528243	2.9121684180771107E-5	0.0	6.697987361577355E-4	6.147911104829455E-5
22	0.03702013207913358	2.9121684180771107E-5	0.0	0.001359011928435985	6.147911104829455E-5
23	0.04217143443644331	3.55931695542758E-5	0.0	0.0031160202073425083	6.795059642179925E-5
24	0.045426591579316174	4.206465492778049E-5	0.0	0.0041870510366575344	7.11863391085516E-5
25	0.045468656234243954	4.206465492778049E-5	0.0	0.004335895200248143	7.442208179530394E-5
26	0.04581164495903971	4.206465492778049E-5	0.0	0.004769484720272957	7.442208179530394E-5
27	0.045886067040835005	4.853614030128518E-5	0.0	0.0052451388952255516	7.442208179530394E-5
28	0.046044618432485876	4.853614030128518E-5	0.0	0.007150991337722683	7.442208179530394E-5
29	0.04635848547310085	4.853614030128518E-5	0.0	0.009438661417256591	7.442208179530394E-5
30	0.04638760715728162	4.853614030128518E-5	0.0	0.013502754231817536	7.442208179530394E-5
31	0.047743383343030854	4.853614030128518E-5	0.0	0.018372546975379815	7.442208179530394E-5
32	0.048765878032044595	4.853614030128518E-5	0.0	0.023339411999544667	7.442208179530394E-5
33	0.05209869299939951	5.8243368361542215E-5	0.0	0.029393486566458304	7.442208179530394E-5
34	0.05479730240015097	5.8243368361542215E-5	0.0	0.03638269076984337	7.765782448205628E-5
35	0.0553991505398869	6.147911104829455E-5	0.0	0.04676618905163164	7.765782448205628E-5
36	0.05560623807183905	6.147911104829455E-5	0.0	0.05775153547315586	7.765782448205628E-5
37	0.055845683030658726	6.147911104829455E-5	0.0	0.07463240506994284	7.765782448205628E-5
38	0.05593304808320104	6.471485373504691E-5	0.0	0.09435425674569839	8.089356716880864E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGA	3610	0.0	18.95213	20
CAATGCG	3810	0.0	18.01428	19
GTACTAG	5115	0.0	17.248503	1
CGACGAG	4320	0.0	16.75386	24
TAGGTCG	4025	0.0	16.342186	21
TGCGACG	4075	0.0	16.249533	22
CATTGCG	3990	0.0	16.042324	29
CGACGAA	3190	0.0	15.999731	38
CGTCATT	4155	0.0	15.989716	26
GTCGACG	3240	0.0	15.888545	36
TCGACGA	3260	0.0	15.790915	37
ATCGTCA	4740	0.0	14.946495	10
TCGTCAG	4650	0.0	14.666468	41
GTCGTCA	4530	0.0	14.665992	24
AGGTCGT	4700	0.0	14.275918	22
CAATTCG	3665	0.0	14.226182	15
GTATTAG	5090	0.0	14.13457	1
GACGAGT	4885	0.0	13.960464	25
AGTCGAC	3710	0.0	13.697816	35
ATTAGGT	5090	0.0	13.570601	19
>>END_MODULE