FastQCFastQC Report
Wed 25 May 2016
SRR2558163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14383228
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1416820.9850500875046964No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1242980.8641870934674747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT848590.5899857806606417No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG562260.39091363913580457No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG558700.3884385341037492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528410.3673792837046037No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC487970.33926320294721046No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG468750.3259004167910013No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA440820.30648196635692626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437230.3039860036981963No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC325000.22595762230842756No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT321740.22369109354311842No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC315980.21968642922159057No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC304080.21141290397398968No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT224350.15598028481506376No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA217670.15133598660884748No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT175170.12178768215313002No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG165810.11528010263064731No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC156260.10864042480589198No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC152480.10601236384488934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGA13650.020.79039620
TGCGACG13900.020.09986322
GTACTAG19600.018.4114131
CAATGCG15350.018.34411219
TCTAGAC47200.017.8986633
CGACGAG16050.017.40735824
CTCTAGT44650.017.09479127
GTTCTAG50750.017.0394631
CTAGACA50750.017.0373884
GTCGACG12050.016.79619836
TAGACAT53700.015.7736215
ATCGTCA18500.015.57939410
TAGGCCG23850.015.4054025
CGACGAA13400.015.26811238
GCCGTAT22450.015.1907228
TCGACGA13450.015.04768537
TAGGTCG14850.014.96232421
AGTCGAC13400.014.9398735
CCTTAGG26250.014.8328282
CCTCTAG58450.014.8292426
Produced by FastQC (version 0.11.5)