##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14383228
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57160256376385	33.0	32.0	34.0	25.0	34.0
2	31.62130288138379	33.0	32.0	34.0	27.0	34.0
3	31.648703197919133	33.0	32.0	34.0	27.0	34.0
4	31.51247480746325	33.0	32.0	34.0	27.0	34.0
5	31.561865180750804	33.0	32.0	34.0	27.0	34.0
6	34.89664114342066	38.0	36.0	38.0	26.0	38.0
7	34.95522312515661	38.0	36.0	38.0	26.0	38.0
8	35.028047459165634	38.0	36.0	38.0	26.0	38.0
9	35.18303818864583	38.0	36.0	38.0	28.0	38.0
10	35.11892511194288	38.0	36.0	38.0	28.0	38.0
11	35.14815074891394	38.0	36.0	38.0	28.0	38.0
12	35.07195985490878	38.0	36.0	38.0	27.0	38.0
13	35.12314683463267	38.0	36.0	38.0	27.0	38.0
14	35.13007226194287	38.0	36.0	38.0	27.0	38.0
15	35.10911521391443	38.0	36.0	38.0	27.0	38.0
16	35.099562490422876	38.0	36.0	38.0	27.0	38.0
17	35.05211820322948	38.0	36.0	38.0	27.0	38.0
18	35.06342741698873	38.0	36.0	38.0	27.0	38.0
19	35.070608628327385	38.0	36.0	38.0	27.0	38.0
20	35.03577256788254	38.0	36.0	38.0	27.0	38.0
21	35.019406909213984	38.0	36.0	38.0	26.0	38.0
22	35.04788890226867	38.0	36.0	38.0	27.0	38.0
23	35.043356609517694	38.0	36.0	38.0	27.0	38.0
24	35.04813794233117	38.0	36.0	38.0	27.0	38.0
25	35.05278988833383	38.0	36.0	38.0	27.0	38.0
26	35.03774702034898	38.0	36.0	38.0	27.0	38.0
27	35.044827697927055	38.0	36.0	38.0	27.0	38.0
28	35.067025427115524	38.0	36.0	38.0	27.0	38.0
29	35.0625904699557	38.0	36.0	38.0	27.0	38.0
30	35.06536328284582	38.0	36.0	38.0	27.0	38.0
31	35.09286225595534	38.0	36.0	38.0	27.0	38.0
32	35.11046157371628	38.0	36.0	38.0	27.0	38.0
33	35.079635878677585	38.0	36.0	38.0	27.0	38.0
34	35.0578012112441	38.0	36.0	38.0	27.0	38.0
35	35.045920498513965	38.0	36.0	38.0	27.0	38.0
36	35.02363982549675	38.0	36.0	38.0	27.0	38.0
37	35.024798258082264	38.0	36.0	38.0	27.0	38.0
38	34.99019914027644	38.0	36.0	38.0	25.0	38.0
39	34.99645851404149	38.0	36.0	38.0	25.0	38.0
40	34.91533145410752	38.0	36.0	38.0	25.0	38.0
41	34.961029679846554	38.0	36.0	38.0	25.0	38.0
42	34.84067978342553	38.0	36.0	38.0	25.0	38.0
43	34.91703371454586	38.0	36.0	38.0	25.0	38.0
44	34.90150437718153	38.0	36.0	38.0	25.0	38.0
45	34.91307973425715	38.0	36.0	38.0	25.0	38.0
46	34.887722005102056	38.0	36.0	38.0	25.0	38.0
47	34.82086628954224	38.0	36.0	38.0	25.0	38.0
48	34.772738567448144	38.0	36.0	38.0	25.0	38.0
49	34.699163497929675	38.0	36.0	38.0	25.0	38.0
50	34.34458217585093	38.0	35.0	38.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	38.0
4	745.0
5	1717.0
6	2597.0
7	1305.0
8	536.0
9	359.0
10	360.0
11	336.0
12	313.0
13	319.0
14	318.0
15	427.0
16	957.0
17	3000.0
18	16118.0
19	51634.0
20	97347.0
21	132109.0
22	153458.0
23	170999.0
24	188573.0
25	207389.0
26	228440.0
27	250935.0
28	279884.0
29	315274.0
30	357415.0
31	405487.0
32	470132.0
33	561010.0
34	712035.0
35	1001924.0
36	1913729.0
37	6856009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71087417895413	17.994560253799186	13.472093133885247	24.82247243336144
2	14.959590434080583	20.8568479898949	38.20561698667365	25.977944589350876
3	18.23358182680804	26.481880815462027	29.44546168003689	25.839075677693042
4	12.71045901340263	17.36843773917377	36.02486100110354	33.896242246320064
5	13.88711438204532	36.77848215615725	34.0160583249221	15.318345136875328
6	31.435490365945736	35.16687923543148	18.495722789922596	14.90190760870019
7	27.136227565425404	31.302336268413583	23.03416671599935	18.527269450161665
8	27.776565538040547	31.64505833872811	21.1625190440698	19.415857079161544
9	27.67587745443052	14.015721883469448	20.107363698652346	38.201036963447685
10	16.84934256924586	27.80846325322018	31.43862361754443	23.903570559989536
11	32.84505640044939	23.360836934045494	22.667140004511648	21.126966660993478
12	24.12505975155876	25.405882684467933	29.689284936484178	20.779772627489127
13	29.604126126776737	20.697710160215664	25.272199509795634	24.425964203211965
14	23.821840808150984	20.344891270824775	26.68994092294263	29.14332699808161
15	26.06105628781995	25.699109155437444	24.731319256629707	23.508515300112904
16	22.890442721783888	26.114492147844054	27.026725047642795	23.968340082729263
17	23.311480740598146	26.084859096798308	27.209563444652822	23.394096717950724
18	24.145110065057008	24.025762580538505	29.54857386261562	22.28055349178887
19	23.59143635049204	25.7605217399775	29.1916175372654	21.456424372265065
20	24.594712368742773	24.185909330540582	29.052728460226714	22.16664984048993
21	24.019753258885597	24.954118330321865	28.253271269128028	22.772857141664506
22	23.970820052118476	24.714767505116544	27.030825867794928	24.28358657497005
23	22.435989127884827	25.356346459480168	27.602240544017626	24.605423868617386
24	22.61521877442137	26.861540464986337	27.297874717447762	23.22536604314453
25	23.860018501369584	24.04247203175774	26.991107807941116	25.10640165893156
26	24.2555076256756	24.16006229415338	27.851568452938064	23.732861627232953
27	23.763670016644603	24.42782612470734	27.088631883778874	24.719871974869182
28	22.98269361714087	24.516619765183712	28.392510455093255	24.10817616258216
29	23.38579221956935	23.857444984742468	29.019197932215736	23.737564863472453
30	23.071471052890995	25.477808124298186	28.914043131171894	22.536677691638925
31	25.12662389246701	23.71510451983925	27.215761603445383	23.942509984248357
32	22.326694928813616	24.883248208340348	28.46180227534863	24.32825458749741
33	23.52521459809656	25.242507188051906	27.870914747603585	23.361363466247948
34	22.63010573797667	24.644805071126637	27.908121307132365	24.816967883764327
35	22.737854164053562	25.954340899495428	28.921687811863922	22.386117124587095
36	24.491477174700666	24.271985483258778	27.783776022332763	23.452761319707797
37	24.05595554620921	25.2834517676523	27.881544858133072	22.77904782800542
38	24.49831075932958	24.789017131490237	27.452571908408462	23.26010020077172
39	24.450762447550474	23.740672258222304	27.37082436654688	24.437740927680345
40	23.609335499948124	25.477313621248726	27.510425030343207	23.40292584845994
41	23.004621831492045	25.27173349641587	27.239758465298692	24.48388620679339
42	24.93428938052383	25.511909728010213	27.58796874386898	21.965832147596977
43	24.96316428464693	23.758959879053634	26.73524393939436	24.542631896905075
44	23.806959928649228	25.4944295827931	27.187813591241856	23.510796897315817
45	25.761293540370882	23.983928508228384	26.77560064189734	23.47917730950339
46	22.592441503388926	24.77238367688693	29.194192707864357	23.44098211185979
47	24.690885551203632	24.93273832576942	27.55116330329324	22.825212819733707
48	24.85828664489225	25.155975436676286	26.861201644694066	23.124536273737395
49	23.595523907767674	25.4673656708688	28.23365789683657	22.703452524526956
50	24.34133569248158	25.5473700428963	26.32662457504183	23.784669689580294
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2054.0
1	2594.5
2	3135.0
3	9301.0
4	15467.0
5	17312.5
6	19158.0
7	16649.0
8	14140.0
9	18349.0
10	22558.0
11	31078.5
12	39599.0
13	70500.5
14	101402.0
15	122987.5
16	144573.0
17	130541.0
18	116509.0
19	96286.5
20	76064.0
21	80401.5
22	84739.0
23	94178.0
24	103617.0
25	123871.5
26	144126.0
27	161706.5
28	179287.0
29	212371.5
30	245456.0
31	272062.5
32	298669.0
33	325856.0
34	353043.0
35	395572.0
36	438101.0
37	478961.5
38	519822.0
39	570532.0
40	621242.0
41	650813.0
42	680384.0
43	710391.0
44	740398.0
45	832656.0
46	924914.0
47	1120357.0
48	1315800.0
49	1383629.5
50	1451459.0
51	1265323.0
52	1079187.0
53	947184.5
54	815182.0
55	804309.5
56	793437.0
57	750877.0
58	708317.0
59	690011.0
60	671705.0
61	590648.0
62	509591.0
63	441426.0
64	373261.0
65	312724.0
66	252187.0
67	214369.5
68	176552.0
69	149177.5
70	121803.0
71	101689.0
72	81575.0
73	68210.0
74	54845.0
75	43533.0
76	32221.0
77	25821.5
78	19422.0
79	16307.5
80	13193.0
81	11325.5
82	9458.0
83	8361.0
84	7264.0
85	5627.0
86	3990.0
87	3953.0
88	3916.0
89	2079.0
90	242.0
91	198.0
92	154.0
93	80.5
94	7.0
95	4.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03209293491002159
2	0.0
3	1.390508444974939E-4
4	0.0026419660454523835
5	0.025348968951893133
6	0.06841301549276699
7	0.05589148694576766
8	0.07856372714108405
9	0.09173184211499671
10	0.08699716085985705
11	0.08019062202170472
12	0.07799361867864432
13	0.06979657139551705
14	0.08929845233629058
15	0.07430181875723586
16	0.06671659518989757
17	0.06601438842518523
18	0.07585223567338292
19	0.07868887290113179
20	0.07495535772637409
21	0.06463083252243516
22	0.057622669959761466
23	0.06657754434540007
24	0.055689863221246305
25	0.06366442915317758
26	0.08828338117145887
27	0.06813491380377201
28	0.05851259536454543
29	0.05585672423464329
30	0.061815052921360907
31	0.05272808023344968
32	0.057622669959761466
33	0.05550909712339956
34	0.05479993781646234
35	0.05957633432495126
36	0.06015339532961586
37	0.05583586660796867
38	0.08121959827098618
39	0.048848561671969604
40	0.08208866604909551
41	0.07048487307577965
42	0.06674440535879707
43	0.05973624279612338
44	0.07935631695471976
45	0.010352335372838419
46	0.008628104901069496
47	0.00930250149688234
48	0.016950297944244505
49	0.05507108696323245
50	0.004421816855020306
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.4383228E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.218116680547848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.16134072777325	18.552233250311108
2	13.444575234115113	7.318720348850983
3	6.2662618228647435	5.116675363367851
4	3.4024078448269734	3.7042853486124767
5	2.064641711689401	2.809782950614408
6	1.2922230326441935	2.1103126367880627
7	0.8878933148848132	1.6916748691089283
8	0.6453886267484663	1.4053010357710642
9	0.4918159275602454	1.2047672971517966
>10	2.815582296925982	14.273731218760396
>50	0.2630404716148278	4.987607718963045
>100	0.21440498880583017	11.891861938615198
>500	0.02867471942170989	5.462384076503627
>1k	0.019949698208756996	10.005207075137331
>5k	9.254992711084567E-4	1.7510649981363586
>10k+	8.740826449357647E-4	7.714389873307185
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	141682	0.9850500875046964	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	124298	0.8641870934674747	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84859	0.5899857806606417	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	56226	0.39091363913580457	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	55870	0.3884385341037492	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52841	0.3673792837046037	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	48797	0.33926320294721046	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	46875	0.3259004167910013	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	44082	0.30648196635692626	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43723	0.3039860036981963	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	32500	0.22595762230842756	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	32174	0.22369109354311842	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	31598	0.21968642922159057	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	30408	0.21141290397398968	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	22435	0.15598028481506376	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	21767	0.15133598660884748	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	17517	0.12178768215313002	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	16581	0.11528010263064731	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	15626	0.10864042480589198	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	15248	0.10601236384488934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1715253349248166E-5	0.0	0.0	0.0	0.0
2	3.615321956934841E-4	0.0	0.0	6.952542224874694E-6	0.0
3	6.048711735640984E-4	6.952542224874694E-6	0.0	6.952542224874694E-6	0.0
4	6.118237157889731E-4	6.952542224874694E-6	0.0	6.952542224874694E-6	0.0
5	6.952542224874694E-4	6.952542224874694E-6	0.0	1.3905084449749389E-5	0.0
6	7.230643913869682E-4	6.952542224874694E-6	0.0	1.3905084449749389E-5	0.0
7	8.899254047839609E-4	6.952542224874694E-6	0.0	2.7810168899498777E-5	0.0
8	0.0016894677606445507	6.952542224874694E-6	0.0	2.7810168899498777E-5	0.0
9	0.0016894677606445507	6.952542224874694E-6	0.0	2.7810168899498777E-5	0.0
10	0.016074277623910293	6.952542224874694E-6	0.0	1.0428813337312041E-4	0.0
11	0.016136850503934166	6.952542224874694E-6	0.0	1.251457600477445E-4	0.0
12	0.017986226735750833	6.952542224874694E-6	0.0	1.390508444974939E-4	0.0
13	0.018000131820200584	6.952542224874694E-6	0.0	1.6686101339699266E-4	0.0
14	0.018041847073549833	6.952542224874694E-6	0.0	1.7381355562186735E-4	0.0
15	0.018271280966970695	6.952542224874694E-6	0.0	1.8771864007161674E-4	0.0
16	0.018340806389219443	6.952542224874694E-6	0.0	2.0162372452136614E-4	0.0
17	0.020899341927973332	6.952542224874694E-6	0.0	2.711491467701131E-4	0.0
18	0.021198301243642942	6.952542224874694E-6	0.0	2.8505423121986246E-4	0.0
19	0.021573738523786176	6.952542224874694E-6	0.0	2.989593156696119E-4	0.0
20	0.02194917580392941	6.952542224874694E-6	0.0	3.754372801432335E-4	0.0
21	0.023318826622229723	6.952542224874694E-6	0.0	4.658203290666045E-4	0.0
22	0.026857670614690944	6.952542224874694E-6	0.0	0.0012445050582525702	0.0
23	0.030883192562893392	6.952542224874694E-6	0.0	0.003121691458968738	0.0
24	0.033504300981671155	6.952542224874694E-6	0.0	0.004081142286001446	0.0
25	0.03353906369279552	6.952542224874694E-6	0.0	0.00419238296159944	0.0
26	0.033740687417316895	6.952542224874694E-6	0.0	0.0044982948194939275	0.0
27	0.03376849758621639	6.952542224874694E-6	0.0	0.004971067690785406	0.0
28	0.033907548430713884	1.3905084449749389E-5	0.0	0.006389386304659844	0.0
29	0.0340952670707855	1.3905084449749389E-5	0.0	0.008301335416500384	0.0
30	0.03410917215523525	1.3905084449749389E-5	0.0	0.011694176022239235	0.0
31	0.03484614163107197	1.3905084449749389E-5	0.0	0.016317616601780906	0.0
32	0.03547187043131069	1.3905084449749389E-5	0.0	0.021657169030484674	0.0
33	0.03730038903645273	1.3905084449749389E-5	0.0	0.027629402801652034	0.0
34	0.03877432798812617	1.3905084449749389E-5	0.0	0.03452632468872773	0.0
35	0.03912890764159478	1.3905084449749389E-5	0.0	0.045177619377235766	0.0
36	0.0392888161127669	1.3905084449749389E-5	0.0	0.055919297114667164	0.0
37	0.03940700933058977	1.3905084449749389E-5	0.0	0.07184061880963022	0.0
38	0.03953215509063751	1.3905084449749389E-5	0.0	0.09077934383018889	6.952542224874694E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGA	1365	0.0	20.790396	20
TGCGACG	1390	0.0	20.099863	22
GTACTAG	1960	0.0	18.411413	1
CAATGCG	1535	0.0	18.344112	19
TCTAGAC	4720	0.0	17.898663	3
CGACGAG	1605	0.0	17.407358	24
CTCTAGT	4465	0.0	17.094791	27
GTTCTAG	5075	0.0	17.039463	1
CTAGACA	5075	0.0	17.037388	4
GTCGACG	1205	0.0	16.796198	36
TAGACAT	5370	0.0	15.773621	5
ATCGTCA	1850	0.0	15.579394	10
TAGGCCG	2385	0.0	15.405402	5
CGACGAA	1340	0.0	15.268112	38
GCCGTAT	2245	0.0	15.190722	8
TCGACGA	1345	0.0	15.047685	37
TAGGTCG	1485	0.0	14.962324	21
AGTCGAC	1340	0.0	14.93987	35
CCTTAGG	2625	0.0	14.832828	2
CCTCTAG	5845	0.0	14.82924	26
>>END_MODULE