##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27466011
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.703424534418193	33.0	32.0	34.0	27.0	34.0
2	31.75213324570503	33.0	32.0	34.0	27.0	34.0
3	31.773964810543475	33.0	32.0	34.0	27.0	34.0
4	31.636715757523	33.0	32.0	34.0	27.0	34.0
5	31.698668110196273	33.0	32.0	34.0	27.0	34.0
6	35.038908817155864	38.0	36.0	38.0	28.0	38.0
7	35.11560353631257	38.0	36.0	38.0	28.0	38.0
8	35.19685898327209	38.0	36.0	38.0	28.0	38.0
9	35.34182339037147	38.0	37.0	38.0	28.0	38.0
10	35.258546900021265	38.0	37.0	38.0	28.0	38.0
11	35.285600082225265	38.0	37.0	38.0	28.0	38.0
12	35.219328463823885	38.0	36.0	38.0	28.0	38.0
13	35.27070873160285	38.0	37.0	38.0	28.0	38.0
14	35.28521345891837	38.0	37.0	38.0	28.0	38.0
15	35.27504612883174	38.0	37.0	38.0	28.0	38.0
16	35.2715914225768	38.0	37.0	38.0	28.0	38.0
17	35.21170573331526	38.0	36.0	38.0	28.0	38.0
18	35.22411059254291	38.0	37.0	38.0	28.0	38.0
19	35.24516457813987	38.0	37.0	38.0	28.0	38.0
20	35.20310302795699	38.0	36.0	38.0	27.0	38.0
21	35.18064694578328	38.0	36.0	38.0	27.0	38.0
22	35.201916288462854	38.0	37.0	38.0	27.0	38.0
23	35.195871144157046	38.0	36.0	38.0	27.0	38.0
24	35.201995113160045	38.0	37.0	38.0	27.0	38.0
25	35.21011682402661	38.0	37.0	38.0	27.0	38.0
26	35.18201601972707	38.0	36.0	38.0	27.0	38.0
27	35.18226665677808	38.0	36.0	38.0	27.0	38.0
28	35.20504866906228	38.0	37.0	38.0	27.0	38.0
29	35.19807222825331	38.0	36.0	38.0	27.0	38.0
30	35.18665415229026	38.0	36.0	38.0	27.0	38.0
31	35.22025852243342	38.0	37.0	38.0	27.0	38.0
32	35.22948366983469	38.0	37.0	38.0	27.0	38.0
33	35.190020531193994	38.0	36.0	38.0	27.0	38.0
34	35.163682305377364	38.0	36.0	38.0	27.0	38.0
35	35.14090957729537	38.0	36.0	38.0	27.0	38.0
36	35.1151604796197	38.0	36.0	38.0	27.0	38.0
37	35.10087646145631	38.0	36.0	38.0	27.0	38.0
38	35.06063097404279	38.0	36.0	38.0	27.0	38.0
39	35.05841740178434	38.0	36.0	38.0	26.0	38.0
40	34.953666624541874	38.0	36.0	38.0	25.0	38.0
41	34.98531887284251	38.0	36.0	38.0	25.0	38.0
42	34.85805918449534	38.0	36.0	38.0	25.0	38.0
43	34.92302806548792	38.0	36.0	38.0	25.0	38.0
44	34.89678923524789	38.0	36.0	38.0	25.0	38.0
45	34.88468693178635	38.0	36.0	38.0	25.0	38.0
46	34.82618852078666	38.0	36.0	38.0	25.0	38.0
47	34.725349196139184	38.0	36.0	38.0	25.0	38.0
48	34.658870922319224	38.0	36.0	38.0	24.0	38.0
49	34.566202096110715	38.0	36.0	38.0	24.0	38.0
50	34.20784878444853	38.0	35.0	38.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	52.0
4	1360.0
5	3413.0
6	5184.0
7	2619.0
8	968.0
9	717.0
10	640.0
11	595.0
12	610.0
13	597.0
14	636.0
15	843.0
16	1919.0
17	6022.0
18	27652.0
19	85602.0
20	160166.0
21	222875.0
22	268807.0
23	306114.0
24	342907.0
25	381294.0
26	423027.0
27	469116.0
28	527766.0
29	598724.0
30	682018.0
31	774094.0
32	896428.0
33	1073494.0
34	1359586.0
35	1905500.0
36	3598963.0
37	1.3335703E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87462139976957	18.833737564492516	13.710174089274375	23.581466946463536
2	13.805499458949463	21.52789496807527	39.57244100717793	25.09416456579734
3	18.26807734854062	28.87629528054744	29.468741326764423	23.386886044147516
4	12.13042892383664	18.285737005275635	36.57411370317599	33.00972036771173
5	12.494663414063895	38.405716005679004	34.54382136881358	14.555799211443517
6	29.749988186177678	35.734952551426744	19.449358925709838	15.065700336685738
7	24.949239916589345	32.78108135489378	24.142553949767454	18.127124778749423
8	27.865267265638177	32.1422855879398	21.294302871760504	18.69814427466152
9	27.78520563744423	14.152068517194968	20.6632260742947	37.39949977106611
10	16.69169662524103	29.036128193132647	31.922493225840796	22.349681955785524
11	31.30270673004589	24.472072294905132	22.327406473879428	21.89781450116955
12	23.414973514874827	26.732143801921026	30.633851725566792	19.219030957637354
13	29.927138064950526	21.586505057306177	25.363797768963032	23.122559108780266
14	23.199347135972328	21.399393956532677	27.83104774560168	27.57021116189331
15	26.532189191177423	25.424935100652057	25.851104479469644	22.19177122870087
16	21.227344626906085	26.795484679177793	29.15650660945362	22.820664084462503
17	22.2671316792611	26.820467034388322	28.470015489894696	22.442385796455877
18	23.53705900982907	24.240939494509618	31.36364755969783	20.858353935963482
19	22.05752596679732	26.882586201298942	31.7507086888863	19.309179143017438
20	23.73215533074555	24.250845351234087	31.343947322329036	20.673051995691324
21	22.545896640588694	25.68500989969511	29.996988831351423	21.772104628364772
22	22.747205876706296	25.424760584123806	28.3809352692797	23.447098269890194
23	20.855286254727776	26.420307197352084	29.217671549757114	23.50673499816303
24	21.342740752117493	28.39676387863605	28.76704274387047	21.493452625375987
25	22.36947615294392	24.59230185696466	28.449370277591996	24.588851712499427
26	23.03377485215107	25.00166720199338	29.397577786907608	22.566980158947942
27	22.954407650978574	24.705406909836842	28.30818204877632	24.032003390408267
28	21.767900761063107	25.153666011296544	30.25509015076306	22.823343076877286
29	22.47147979252308	23.824151865491913	31.302793358121505	22.401574983863497
30	21.971831170639028	26.22735007318559	30.941327695958304	20.859491060217074
31	24.986239210211174	23.80756518133679	28.27391865692069	22.932276951531346
32	19.991931489500665	25.82119353044475	30.795280161795713	23.391594818258877
33	22.742130691286658	26.345000099633754	28.949892674230743	21.962976534848842
34	20.365298240851423	25.57616999164975	30.225773028603385	23.832758738895443
35	20.788945359968277	27.51097552676481	31.201378456505847	20.498700656761066
36	23.483391272583166	25.122028355483316	29.269005131050857	22.12557524088266
37	22.407875384636178	26.616971018631457	29.56253906379353	21.412614532938832
38	23.470871262221863	25.78919429417268	28.806957282962415	21.93297716064305
39	23.41905327043125	24.035922088547398	29.188378049255814	23.35664659176554
40	22.100479362963817	26.309040003078373	29.433072546354843	22.15740808760297
41	21.091831024095068	26.184706998544065	28.891106554330857	23.832355423030005
42	23.489714237101833	26.72504741340757	29.21899174074926	20.566246608741338
43	23.93832455193214	24.01706972366729	28.125961090012417	23.91864463438815
44	22.538546074921168	26.22909817816642	29.29660928633009	21.935746460582326
45	25.592709379509	24.2679692425355	28.314497528722338	21.824823849233162
46	21.227383493621108	25.356914828250094	31.627117687486628	21.78858399064217
47	23.882028445130608	25.990041291318626	29.01633082575355	21.111599437797214
48	24.002121099996167	25.867311153621962	28.616103514399192	21.51446423198268
49	22.703521936062657	26.397387616972797	30.526470749597106	20.37261969736744
50	22.98600904592382	26.556462601183352	27.6863802053731	22.77114814751973
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3538.0
1	4911.5
2	6285.0
3	20711.5
4	35138.0
5	40459.0
6	45780.0
7	40280.5
8	34781.0
9	45974.5
10	57168.0
11	79297.5
12	101427.0
13	186166.0
14	270905.0
15	325397.0
16	379889.0
17	348024.0
18	316159.0
19	263365.0
20	210571.0
21	218802.5
22	227034.0
23	252986.5
24	278939.0
25	350425.5
26	421912.0
27	456693.5
28	491475.0
29	559752.0
30	628029.0
31	688914.5
32	749800.0
33	797946.0
34	846092.0
35	928005.0
36	1009918.0
37	1062576.5
38	1115235.0
39	1149261.0
40	1183287.0
41	1228018.0
42	1272749.0
43	1348744.0
44	1424739.0
45	1628810.5
46	1832882.0
47	2204193.0
48	2575504.0
49	2807408.5
50	3039313.0
51	2544742.5
52	2050172.0
53	1668798.0
54	1287424.0
55	1277506.0
56	1267588.0
57	1196241.5
58	1124895.0
59	1086679.5
60	1048464.0
61	848908.0
62	649352.0
63	560166.0
64	470980.0
65	397115.0
66	323250.0
67	275698.0
68	228146.0
69	192842.0
70	157538.0
71	129950.5
72	102363.0
73	89008.0
74	75653.0
75	60185.5
76	44718.0
77	37059.5
78	29401.0
79	24137.5
80	18874.0
81	15281.0
82	11688.0
83	10215.0
84	8742.0
85	6627.0
86	4512.0
87	3888.5
88	3265.0
89	1777.5
90	290.0
91	202.5
92	115.0
93	68.5
94	22.0
95	13.0
96	4.0
97	3.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032582088458349485
2	0.0
3	1.8204318056961382E-4
4	0.0025194776190834555
5	0.025398664553072523
6	0.07044342915321777
7	0.05752564505999798
8	0.08038662767593008
9	0.09384325958363593
10	0.08821448444042347
11	0.08202137543744521
12	0.07983321640699845
13	0.07129175037467217
14	0.09129465505566134
15	0.0758027803891872
16	0.0679348741249685
17	0.0671338841304622
18	0.07753219060459854
19	0.08017909845008071
20	0.07613045811421251
21	0.06624915427289386
22	0.058534164280353636
23	0.06786205685274065
24	0.05686664874633597
25	0.06511684568975087
26	0.09038443915281327
27	0.06979171456677855
28	0.05964462768182827
29	0.057616666650282776
30	0.06299422220430917
31	0.053975803038890505
32	0.058570572916467556
33	0.056338723522684084
34	0.05586905211681449
35	0.06101723326332317
36	0.061377678760851
37	0.0572343759710866
38	0.08289882356779076
39	0.05032037597305265
40	0.08345951656394517
41	0.071837879916381
42	0.0677419083535647
43	0.06083519008275355
44	0.08094003894486171
45	0.010518454973312288
46	0.008687100576781973
47	0.009506294889345235
48	0.01743245497134622
49	0.057616666650282776
50	0.00460569246841123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.7466011E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.21877642322348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.64960513363579	13.293479643338518
2	11.56555635885638	4.908139091775284
3	6.75383919109118	4.299246113825049
4	4.474274398264943	3.797545124517464
5	3.020435875266252	3.2044976768978968
6	2.1583444038765354	2.7478456410103065
7	1.5877780278705271	2.3583494888163985
8	1.2060310557823832	2.0472402665688447
9	0.9749923314229577	1.8619329865339036
>10	5.126676423687792	18.92171155506352
>50	0.2582202633763177	3.7695464681135307
>100	0.1745552836343788	7.390092168042768
>500	0.024137336422695912	3.5971255611376103
>1k	0.021927254011298178	9.432784716488467
>5k	0.0019422412154825972	2.8403772191252306
>10k+	0.0016844215851086995	15.530086278745397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	430517	1.5674536793857687	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	377552	1.374615338208377	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	218077	0.7939886137815936	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	170656	0.6213352204657604	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	161497	0.5879885506490186	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144719	0.5269021409770789	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	137751	0.5015326033328975	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	127964	0.46589947116820135	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117770	0.4287845075136684	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	113574	0.41350744380026644	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	102113	0.37177950595009956	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	96825	0.3525266191730572	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	95899	0.34915517946890795	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	95701	0.34843428847385227	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	62242	0.22661463290027808	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	55908	0.20355340278571943	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	52714	0.1919244844109325	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	52224	0.19014046124135028	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	46162	0.1680695460290903	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39660	0.1443966508278177	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	39165	0.14259442334017852	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	38963	0.14185896889067728	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	38902	0.14163687621038235	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	37592	0.13686734487945848	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	37284	0.13574595888714966	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	36804	0.13399834435368135	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	33511	0.1220089804813666	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	33354	0.121437364894378	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	32355	0.11780014214659712	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	31422	0.11440321639716812	No Hit
GTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCACTTTTTCGAACTG	31025	0.1129577935434454	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	30939	0.11264467927286564	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	30366	0.11055846442353788	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	29420	0.10711420744716078	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	29149	0.10612753340847347	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	28333	0.10315658870157739	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	28003	0.10195510370981792	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.281727222784554E-5	0.0	0.0	0.0	0.0
2	8.373986306202236E-4	0.0	0.0	3.6408636113922766E-6	0.0
3	0.0011759989464797053	0.0	0.0	7.281727222784553E-6	0.0
4	0.0011796398100910976	0.0	0.0	7.281727222784553E-6	0.0
5	0.0013216334909353965	0.0	0.0	1.4563454445569106E-5	0.0
6	0.0013616829906607116	0.0	0.0	1.4563454445569106E-5	0.0
7	0.0017184876245771546	0.0	0.0	1.8204318056961384E-5	0.0
8	0.0033969257494289944	0.0	0.0	2.184518166835366E-5	0.0
9	0.00342241179470874	0.0	0.0	2.548604527974594E-5	3.6408636113922766E-6
10	0.03379449604094311	0.0	0.0	1.1650763556455285E-4	3.6408636113922766E-6
11	0.033940130585398805	0.0	0.0	1.3107109001012196E-4	3.6408636113922766E-6
12	0.037311570289548054	0.0	0.0	1.6383886251265244E-4	3.6408636113922766E-6
13	0.03736618324371894	0.0	0.0	1.7840231695822155E-4	3.6408636113922766E-6
14	0.03746084569761514	0.0	0.0	1.9296577140379066E-4	3.6408636113922766E-6
15	0.03798877092126702	0.0	0.0	2.2209268029492888E-4	3.6408636113922766E-6
16	0.03808707423877461	0.0	0.0	2.694239072430285E-4	3.6408636113922766E-6
17	0.04383235701755162	0.0	0.0	3.932132700303659E-4	3.6408636113922766E-6
18	0.044527761967327543	0.0	0.0	4.4782622420125003E-4	3.6408636113922766E-6
19	0.04512486359959588	0.0	0.0	5.024391783721342E-4	3.6408636113922766E-6
20	0.04584575459465155	0.0	0.0	5.461295417088415E-4	3.6408636113922766E-6
21	0.04820503421483374	0.0	0.0	7.318135858898476E-4	3.6408636113922766E-6
22	0.05527923221176894	0.0	0.0	0.0015437261712303253	3.6408636113922766E-6
23	0.06337287201989397	0.0	0.0	0.0037428077925112605	3.6408636113922766E-6
24	0.0686848920289153	0.0	0.0	0.0052137166915137406	3.6408636113922766E-6
25	0.06874678671030897	0.0	0.0	0.0054103233265289236	3.6408636113922766E-6
26	0.06934024747896592	0.0	0.0	0.0058217409146162505	3.6408636113922766E-6
27	0.06936937438785705	0.0	0.0	0.006422483410495976	3.6408636113922766E-6
28	0.0695295723867583	0.0	0.0	0.008221070034523761	3.6408636113922766E-6
29	0.06979171456677856	0.0	0.0	0.010671371244990763	3.6408636113922766E-6
30	0.06982084147566969	0.0	0.0	0.015204246441174149	3.6408636113922766E-6
31	0.07136820851051141	0.0	0.0	0.020658260131039777	3.6408636113922766E-6
32	0.07241313636698099	0.0	0.0	0.026836805679572473	3.6408636113922766E-6
33	0.0757627308894619	0.0	0.0	0.033969257494289945	3.6408636113922766E-6
34	0.0787737250960833	0.0	0.0	0.04251436439022761	3.6408636113922766E-6
35	0.07946184831863644	0.0	0.0	0.055362972074830964	3.6408636113922766E-6
36	0.07976039913477061	0.0	0.0	0.06938029697869123	3.6408636113922766E-6
37	0.0800443864964592	0.0	0.0	0.09070847601422719	3.6408636113922766E-6
38	0.08016089413202376	0.0	0.0	0.1132344991779112	3.6408636113922766E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGCG	5590	0.0	22.863981	19
CGACGAG	5885	0.0	22.279503	24
AATGCGA	6290	0.0	21.124693	20
ATCGTCA	6240	0.0	20.873451	10
GTACTAG	7320	0.0	20.409504	1
TGCGACG	6365	0.0	20.392014	22
GTCGTCA	5790	0.0	20.099386	24
CATTGCG	5680	0.0	19.943388	29
GACGAGT	6665	0.0	19.77117	25
AGGTCGT	5880	0.0	19.267988	22
CGTCATT	6255	0.0	18.464573	26
GTCGACG	5250	0.0	18.437777	36
TCGACGA	5295	0.0	18.364012	37
CGACGAA	5255	0.0	18.336472	38
GCTCGTC	6135	0.0	18.07462	39
GTCAGCG	6275	0.0	18.028555	43
TAGGCAT	9605	0.0	17.683313	5
CGTCATC	7445	0.0	17.672194	12
AATTCGT	5585	0.0	17.646355	16
AGTCGAC	5495	0.0	17.615679	35
>>END_MODULE