FastQCFastQC Report
Wed 25 May 2016
SRR2558158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939372
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA717580.7219570813930699No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT616240.6199989295098322No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507550.5106459442306818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333040.33507147131629644No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG282100.2838207484336032No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG281430.28314666157982615No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC278210.27990702028256914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270250.27189846601978473No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG245630.24712828939293147No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA240950.24241974241430947No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT168400.16942720324785107No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC167850.1688738483678848No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC162130.1631189576162357No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC151840.15276619086195786No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG147550.14845002279822106No Hit
GTCCAGGATCGTGAATGGGGAGGACGCCGTCCCCGGCTCCTGGCCCTGGC136910.13774512112032833No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA120560.12129538968860407No Hit
GTCTGGGAGAAGCGGGCAGGTGTGGCCAGCTTCAGCAGGGTGATGTCATT114180.11487647308099547No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT111200.11187829573136009No Hit
CTCTACCACCATGAATCCACTCCTGATCCTTACCTTTGTGGCAGCTGCTC106800.10745145669163No Hit
TCTTAAGGCAGGGGTGGCAGGAGCTGCGGGCTCAGTTGGCGGCCAGGATC101330.10194809088542012No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCTTGTTCTTCTGGGCACAGCCAT100720.10133437001854846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG7800.021.43316324
TCTAAAC12050.020.0832563
CAATGCG7950.019.92118519
AATGCGA7850.019.89474920
TCTAGAC21850.019.3320663
CTCTAGT20700.019.12818527
TGCGACG8100.019.00915522
CTAGACA23200.018.397174
CCGGCTC38750.018.39267733
GAATCTA45600.018.04382913
AATCTAC46200.017.71407714
CGGCTCC40950.017.67304834
CTAAACA14850.017.630224
CTCTACC80900.017.5764521
TAGACAT24950.017.5473295
GACGAGT9350.017.40954425
GTACTAG11650.017.1934361
TAAACAG18000.016.9891175
CTTACCT82200.016.7792229
CCTTACC82250.016.7153628
Produced by FastQC (version 0.11.5)