##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9939372
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.803605096981983	33.0	32.0	34.0	27.0	34.0
2	31.846823823476978	33.0	32.0	34.0	27.0	34.0
3	31.847207851763674	33.0	32.0	34.0	27.0	34.0
4	31.73317660310933	33.0	32.0	34.0	27.0	34.0
5	31.784308304387842	33.0	32.0	34.0	28.0	34.0
6	35.28491940939528	38.0	36.0	38.0	29.0	38.0
7	35.34286351290605	38.0	36.0	38.0	29.0	38.0
8	35.39594211787224	38.0	37.0	38.0	29.0	38.0
9	35.58782898959814	38.0	37.0	38.0	29.0	38.0
10	35.515538003809496	38.0	37.0	38.0	29.0	38.0
11	35.58264023119368	38.0	37.0	38.0	29.0	38.0
12	35.48803858030467	38.0	37.0	38.0	29.0	38.0
13	35.52571852628114	38.0	37.0	38.0	29.0	38.0
14	35.55349995955479	38.0	37.0	38.0	29.0	38.0
15	35.53160682586385	38.0	37.0	38.0	29.0	38.0
16	35.51288190038566	38.0	37.0	38.0	29.0	38.0
17	35.46794515790334	38.0	37.0	38.0	28.0	38.0
18	35.47324690131328	38.0	37.0	38.0	28.0	38.0
19	35.49402980389505	38.0	37.0	38.0	28.0	38.0
20	35.42159303424804	38.0	37.0	38.0	28.0	38.0
21	35.455911198413745	38.0	37.0	38.0	28.0	38.0
22	35.48569577635287	38.0	37.0	38.0	28.0	38.0
23	35.494603985040506	38.0	37.0	38.0	28.0	38.0
24	35.492087025216485	38.0	37.0	38.0	28.0	38.0
25	35.49522102603665	38.0	37.0	38.0	28.0	38.0
26	35.48706216046648	38.0	37.0	38.0	28.0	38.0
27	35.50146115871305	38.0	37.0	38.0	28.0	38.0
28	35.49897065931329	38.0	37.0	38.0	28.0	38.0
29	35.45951545027191	38.0	37.0	38.0	28.0	38.0
30	35.477563270596974	38.0	37.0	38.0	28.0	38.0
31	35.52526749174898	38.0	37.0	38.0	29.0	38.0
32	35.544552814805606	38.0	37.0	38.0	29.0	38.0
33	35.50484366617931	38.0	37.0	38.0	29.0	38.0
34	35.471879209269964	38.0	37.0	38.0	28.0	38.0
35	35.49155489904191	38.0	37.0	38.0	28.0	38.0
36	35.4741550069763	38.0	37.0	38.0	28.0	38.0
37	35.466094236134836	38.0	37.0	38.0	28.0	38.0
38	35.460243765903925	38.0	37.0	38.0	28.0	38.0
39	35.462346916887704	38.0	37.0	38.0	28.0	38.0
40	35.4504900309597	38.0	37.0	38.0	28.0	38.0
41	35.45091239164808	38.0	37.0	38.0	28.0	38.0
42	35.35230243922855	38.0	37.0	38.0	28.0	38.0
43	35.39065144155989	38.0	37.0	38.0	28.0	38.0
44	35.37640738267971	38.0	37.0	38.0	28.0	38.0
45	35.38643407249472	38.0	37.0	38.0	28.0	38.0
46	35.35233966492048	38.0	37.0	38.0	28.0	38.0
47	35.288405243309136	38.0	37.0	38.0	27.0	38.0
48	35.24748736640504	38.0	37.0	38.0	27.0	38.0
49	35.17109873742526	38.0	37.0	38.0	27.0	38.0
50	34.86494629640585	38.0	36.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	20.0
4	437.0
5	1281.0
6	1999.0
7	954.0
8	270.0
9	173.0
10	156.0
11	145.0
12	118.0
13	117.0
14	94.0
15	173.0
16	482.0
17	1472.0
18	7298.0
19	22665.0
20	42786.0
21	60423.0
22	75316.0
23	88922.0
24	102909.0
25	117451.0
26	133576.0
27	150577.0
28	171756.0
29	196587.0
30	225934.0
31	259818.0
32	303934.0
33	368426.0
34	475778.0
35	682230.0
36	1346841.0
37	5098254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.48885460798701	17.142837587002525	12.711153082065238	25.657154722945236
2	16.467006164977022	19.99430145083613	37.47618058766691	26.062511796519942
3	19.158614850113267	24.651607767573243	28.89056773405805	27.299209648255445
4	14.014319901289806	16.34942941973548	34.355838900808614	35.280411778166105
5	15.313557190150837	35.70007748115799	32.947423499934594	16.038941828756577
6	33.812943483931036	33.19449978747605	17.577522919062304	15.415033809530618
7	29.34527282882844	29.40387695328624	21.82818893623396	19.42266128165136
8	28.208484029844705	31.50073085416806	20.127335154075414	20.163449961911812
9	28.58353057148578	13.092667049414889	19.295574143978364	39.02822823512096
10	17.463609907267248	27.329356075956184	29.933182680153298	25.27385133662327
11	34.53504399634462	22.536884774287742	21.723968481258527	21.20410274810911
12	25.32068361092942	23.991619112587166	28.76551747258176	21.92217980390165
13	31.363906690586994	19.84950893624382	24.019269428579165	24.767314944590023
14	25.12784934522107	19.334093054690975	25.593850707000964	29.94420689308699
15	26.674252725700942	25.31745019421365	23.250126621681197	24.758170458404212
16	24.876172906698432	24.38767026665789	25.39282470344219	25.34333212320149
17	23.8023961591059	25.61624343731308	26.265386516296687	24.315973887284333
18	25.628940158426968	23.123721855651628	27.870831776820765	23.37650620910064
19	25.36657982356007	24.4078190406976	27.60008090009714	22.62552023564519
20	26.468389106741224	23.024250587355645	27.492865001879075	23.01449530402406
21	25.519623068282332	23.23757220440665	27.290039179491764	23.952765547819254
22	25.119895778776723	23.3959513614159	25.7976316890661	25.686521170741273
23	23.893042669886565	24.006561268283697	26.468808558035967	25.631587503793767
24	24.116978553032627	25.253800808357273	26.109341953609604	24.519878685000492
25	26.142946900722464	23.10833716816538	25.401001281275192	25.347714649836966
26	25.598751852461056	23.483116004075665	26.233606688569715	24.684525454893567
27	25.390242227922684	22.954352767981163	26.01931284026841	25.636092163827744
28	24.140054234804087	23.462803661553885	26.807162456792334	25.58997964684969
29	25.045431907409217	22.959344495926054	27.078040998099567	24.917182598565166
30	24.972737164522165	23.80477142152194	27.41368329086408	23.808808123091815
31	26.598285535732835	22.74219052627425	26.101739993186918	24.55778394480599
32	23.903917821241794	23.608686557590783	27.322466143882483	25.164929477284943
33	24.951009674846787	23.826170064933194	25.99696544875903	25.22585481146099
34	23.964086437220022	23.435939294135366	26.357253493917394	26.242720774727218
35	24.324888627412133	24.429022953444385	27.429911679111648	23.816176740031835
36	25.926220089480527	22.90101525014332	26.692114610371142	24.480650050005007
37	25.581538156128953	23.711348612435305	26.91563334061159	23.791479890824153
38	25.68703225764232	23.09421873340391	26.762189679829774	24.456559329123998
39	25.857337328848146	22.48235149858987	26.332403717737535	25.327907454824448
40	25.195977753601824	24.01124742327923	25.73206452852742	25.060710294591527
41	25.039021248520875	23.41876980700251	26.63608596867084	24.90612297580577
42	26.345647337124657	23.65705567058299	25.78256603316958	24.21473095912277
43	25.902462082892697	23.066799779433836	25.17288856113997	25.857849576533496
44	25.561512195485353	23.666485927186486	26.000461479638215	24.771540397689947
45	26.34421467103919	22.761574230137224	25.640941012154588	25.253270086668994
46	24.635400174613892	23.566043524278665	27.41814432944049	24.38041197166695
47	26.031641957330407	23.453020061292357	26.25191890797533	24.263419073401906
48	25.888951392082532	23.94177676565093	25.469188217689563	24.700083624576973
49	24.75145435900783	24.091338489726848	27.117398350989504	24.039808800275818
50	25.317058647209063	23.842333335110936	25.660371359308048	25.180236658371957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1329.0
1	1666.5
2	2004.0
3	5633.0
4	9262.0
5	10122.0
6	10982.0
7	9556.0
8	8130.0
9	10783.5
10	13437.0
11	18047.0
12	22657.0
13	42360.5
14	62064.0
15	73480.5
16	84897.0
17	76090.0
18	67283.0
19	53260.0
20	39237.0
21	39243.0
22	39249.0
23	42222.5
24	45196.0
25	55654.5
26	66113.0
27	72562.0
28	79011.0
29	93964.5
30	108918.0
31	123112.5
32	137307.0
33	152681.5
34	168056.0
35	197422.5
36	226789.0
37	246917.5
38	267046.0
39	310870.5
40	354695.0
41	380596.5
42	406498.0
43	446056.0
44	485614.0
45	535573.0
46	585532.0
47	697940.5
48	810349.0
49	886014.5
50	961680.0
51	869909.5
52	778139.0
53	696100.5
54	614062.0
55	612996.5
56	611931.0
57	603345.0
58	594759.0
59	574039.0
60	553319.0
61	500014.5
62	446710.0
63	412152.0
64	377594.0
65	341817.0
66	306040.0
67	254580.0
68	203120.0
69	178193.0
70	153266.0
71	136102.5
72	118939.0
73	85226.5
74	51514.0
75	41766.0
76	32018.0
77	21800.5
78	11583.0
79	9677.5
80	7772.0
81	6774.5
82	5777.0
83	5132.5
84	4488.0
85	3667.0
86	2846.0
87	2427.0
88	2008.0
89	1053.0
90	98.0
91	73.5
92	49.0
93	25.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04486198926853729
2	0.0
3	0.0
4	0.016037230521203955
5	0.014809788787460617
6	0.06412880008918069
7	0.06664404954357277
8	0.07201662237815427
9	0.059852876016714136
10	0.07110107157675556
11	0.06655350056321466
12	0.06333398126159279
13	0.0699742398211879
14	0.06824374819656615
15	0.06277056538380896
16	0.06610075566142408
17	0.06935045795649866
18	0.0803068845798306
19	0.06170409961514671
20	0.06331385926595764
21	0.06151294065661291
22	0.051502247828132404
23	0.05349432539601093
24	0.059289460138930314
25	0.06337422525286306
26	0.05322267845493659
27	0.05704585762561257
28	0.04821230154178755
29	0.06413886108699825
30	0.055526646955159735
31	0.055737927909328674
32	0.05157267481285538
33	0.05813244538990994
34	0.0600239129796128
35	0.0608489148006534
36	0.05399737528688935
37	0.057076040619065266
38	0.06727789240607958
39	0.06272026039472112
40	0.05753884651867341
41	0.07005472780372844
42	0.060295559920687136
43	0.04968120722315252
44	0.06143245267407237
45	0.01571527859104177
46	0.008783251094737174
47	0.014367104883487608
48	0.02114821741252868
49	0.11182799074227225
50	0.009165569011804769
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9939372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.131937594352827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88701460850379	17.347729503767436
2	13.836740578229845	6.678147202861842
3	5.367104893101873	3.885559210280402
4	2.455310706708232	2.370056189961176
5	1.415504767028017	1.7079436351214523
6	0.8738357156504016	1.265240937467528
7	0.5712841379608206	0.9650335216139921
8	0.4141394422610837	0.7995189740803645
9	0.3003841357680958	0.6523966096820366
>10	2.1086321289724643	10.092275868631868
>50	0.32191158713057044	5.492434365401593
>100	0.35607969539448975	18.32369620592024
>500	0.05781453865846936	9.632675046564652
>1k	0.03214226312104568	13.431020434720512
>5k	0.001176448846030829	1.9191329153040577
>10k+	9.243526647385086E-4	5.437139378620881
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	71758	0.7219570813930699	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	61624	0.6199989295098322	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50755	0.5106459442306818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33304	0.33507147131629644	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	28210	0.2838207484336032	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	28143	0.28314666157982615	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	27821	0.27990702028256914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27025	0.27189846601978473	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	24563	0.24712828939293147	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	24095	0.24241974241430947	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	16840	0.16942720324785107	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	16785	0.1688738483678848	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	16213	0.1631189576162357	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	15184	0.15276619086195786	No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG	14755	0.14845002279822106	No Hit
GTCCAGGATCGTGAATGGGGAGGACGCCGTCCCCGGCTCCTGGCCCTGGC	13691	0.13774512112032833	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	12056	0.12129538968860407	No Hit
GTCTGGGAGAAGCGGGCAGGTGTGGCCAGCTTCAGCAGGGTGATGTCATT	11418	0.11487647308099547	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	11120	0.11187829573136009	No Hit
CTCTACCACCATGAATCCACTCCTGATCCTTACCTTTGTGGCAGCTGCTC	10680	0.10745145669163	No Hit
TCTTAAGGCAGGGGTGGCAGGAGCTGCGGGCTCAGTTGGCGGCCAGGATC	10133	0.10194809088542012	No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCTTGTTCTTCTGGGCACAGCCAT	10072	0.10133437001854846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.018299345270506E-5	0.0	0.0	0.0	0.0
2	3.4207392579732403E-4	0.0	0.0	0.0	0.0
3	4.527449017905759E-4	0.0	0.0	2.0121995635136706E-5	0.0
4	4.527449017905759E-4	0.0	0.0	2.0121995635136706E-5	0.0
5	5.43293882148691E-4	0.0	0.0	2.0121995635136706E-5	0.0
6	5.43293882148691E-4	0.0	0.0	2.0121995635136706E-5	0.0
7	6.740868537770797E-4	0.0	0.0	3.018299345270506E-5	0.0
8	0.0012676857250136125	0.0	0.0	3.018299345270506E-5	0.0
9	0.0012878077206487492	0.0	0.0	6.036598690541012E-5	0.0
10	0.011620452479291448	0.0	0.0	1.2073197381082024E-4	0.0
11	0.011771367446554973	0.0	0.0	1.2073197381082024E-4	0.0
12	0.013089358160656428	0.0	0.0	1.6097596508109365E-4	0.0
13	0.013129602151926701	0.0	0.0	1.7103696289866202E-4	0.0
14	0.013169846143196974	0.0	0.0	1.8109796071623036E-4	0.0
15	0.013371066099548341	0.0	0.0	2.0121995635136706E-4	0.0
16	0.013421371088636183	0.0	0.0	2.1128095416893543E-4	0.0
17	0.015131740717622804	0.0	0.0	3.219519301621873E-4	0.0
18	0.015322899676156602	0.0	0.0	3.621959214324607E-4	0.0
19	0.015493936639055265	0.0	0.0	3.9237891488516576E-4	0.0
20	0.015675034599771494	0.0	0.0	4.4268390397300754E-4	0.0
21	0.01631893846009587	0.0	0.0	5.231718865135544E-4	0.0
22	0.018985102881751485	0.0	0.0	8.954288057635834E-4	0.0
23	0.022345476152819312	0.0	0.0	0.0018009186093447354	0.0
24	0.02430737072724514	0.0	0.0	0.00256555444347993	0.0
25	0.02430737072724514	0.0	0.0	0.0026561034238380453	0.0
26	0.024599139663954623	0.0	0.0	0.002998177349635369	0.0
27	0.02461926165958976	0.0	0.0	0.0032698242907097147	0.0
28	0.024719871637765446	0.0	0.0	0.004406717044094939	0.0
29	0.024860725607211403	0.0	0.0	0.005825317736372077	0.0
30	0.024860725607211403	0.0	0.0	0.008692702114379057	0.0
31	0.025323531506819544	0.0	0.0	0.012606430265413146	0.0
32	0.025796398404245257	0.0	0.0	0.01679180535752158	0.0
33	0.027043962133623735	0.0	0.0	0.02143998634923816	0.0
34	0.028211037880461663	1.0060997817568353E-5	0.0	0.02694335215544805	0.0
35	0.028462562825900872	1.0060997817568353E-5	0.0	0.035595810278556836	0.0
36	0.02859335579752926	1.0060997817568353E-5	0.0	0.04391625547368586	0.0
37	0.028704026773522514	1.0060997817568353E-5	0.0	0.056874820662713904	1.0060997817568353E-5
38	0.028734209766975216	1.0060997817568353E-5	0.0	0.07134253552437719	1.0060997817568353E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAG	780	0.0	21.433163	24
TCTAAAC	1205	0.0	20.083256	3
CAATGCG	795	0.0	19.921185	19
AATGCGA	785	0.0	19.894749	20
TCTAGAC	2185	0.0	19.332066	3
CTCTAGT	2070	0.0	19.128185	27
TGCGACG	810	0.0	19.009155	22
CTAGACA	2320	0.0	18.39717	4
CCGGCTC	3875	0.0	18.392677	33
GAATCTA	4560	0.0	18.043829	13
AATCTAC	4620	0.0	17.714077	14
CGGCTCC	4095	0.0	17.673048	34
CTAAACA	1485	0.0	17.63022	4
CTCTACC	8090	0.0	17.576452	1
TAGACAT	2495	0.0	17.547329	5
GACGAGT	935	0.0	17.409544	25
GTACTAG	1165	0.0	17.193436	1
TAAACAG	1800	0.0	16.989117	5
CTTACCT	8220	0.0	16.77922	29
CCTTACC	8225	0.0	16.71536	28
>>END_MODULE