FastQCFastQC Report
Wed 25 May 2016
SRR2558157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13674765
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3990272.9179806746221963No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3546712.5936167824456215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300441.682251943634863No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1711611.2516558785470902No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1555051.1371676222589566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1384261.0122733370555181No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1380161.009275113685683No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC1252010.9155623515285273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1186860.8679198509078584No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1143070.8358973627700366No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT968290.7080852943359538No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC944210.6904762165931189No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC938400.686227514695865No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC868710.6352650301485985No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT572270.4184861677696107No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA543100.3971549054042245No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT502230.3672677373249193No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG481340.3519914236186143No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC446530.32653577593472355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415340.3037273401041992No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA408560.29876930243408206No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC385390.2818256840245518No Hit
GTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCACTTTTTCGAACTG378920.2770943412921538No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC373520.27314546173188353No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC371530.27169022648652463No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG367170.2685018718786027No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG361600.2644286757395831No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA359520.26290762583488636No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA340430.24894760531533813No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG307580.224925254657027No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC307560.22491062917717417No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG307560.22491062917717417No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT307320.22473512341893992No Hit
GTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAAAAAA302830.22145170319197444No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG294340.21524318699443828No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG293720.21478979711899984No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT289220.2114990641521079No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA278150.20340386105355376No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC269770.19727578499520834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG267990.19597411728830438No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC257030.18795935432894093No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC251480.1839007836697742No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC244940.17911825175789128No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC243610.17814565734767654No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC241540.17663192018290624No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA238890.17469404410240322No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA236010.17258797500359238No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA235310.17207608320874251No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG230830.16879997572170344No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229870.1680979526887665No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA225000.16453664834459678No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC223870.16371030873291056No Hit
GTATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGT223690.1635786794142349No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC214140.15659501278449756No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT213990.15648532168560117No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA208010.15211230320959812No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA206320.15087645016203202No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205540.15030605644777076No Hit
GTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCACTTTTTC202920.148390118587047No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC200340.14650343168602897No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA197420.14436811162751242No Hit
GTATCAACGCAGAGTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCA196580.1437538414736926No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195870.14323463693891633No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG190700.1394539503969538No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189080.1382692865288727No Hit
TATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTT187820.13734788129814296No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG184860.1351833102799207No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT180620.13208270855111587No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA176920.12937699477833806No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA172500.12614476373085753No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC169600.12402406915219383No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC167910.12278821610462777No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC162770.11902946778244451No Hit
CTCCTCGCCCTTGCTCACTTTTTCGAACTGCGGGTGGCTCCAGCTAGCAG161940.11842251036855113No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA160310.1172305337605436No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT158290.11575336029540544No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT158270.11573873481555258No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA156960.1147807658851907No Hit
TATCAACGCAGAGTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCAC156480.11442975436872224No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT149540.10935471285978224No Hit
GTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAG148420.10853568598802245No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA148330.10846987132868463No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA144090.10536926959987979No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT143480.10492319246436776No Hit
ACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTTCGAAA142250.10402372545341729No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA137980.10090118550483318No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC137200.1003307917905719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA51050.029.90669610
AGTCCTA54650.028.05544329
GTCATCC55000.027.87986813
CGACGAG55250.027.7501624
TTGGACT15150.027.737064
GAGTCCT55400.027.67502628
CAATGCG56850.027.12313319
ACGTTTA14150.026.58294326
CCAATGC59400.025.92551818
CGTCATC59850.025.58374612
TCACGTT14550.025.39868724
TACCCCG5500.025.200255
TTTGGAC16850.024.9378413
CGGCGCT5650.024.5299610
CGCCGAA450.001399585424.4425438
CTACCCC5550.024.1811264
CATCCAA65300.023.98737115
TCATCCA65200.023.85545214
GCATATA8950.023.8533271
GCTTGTA5550.023.78341115
Produced by FastQC (version 0.11.5)