FastQCFastQC Report
Wed 25 May 2016
SRR2558153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10089206
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA472100.46792582092188423No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT388280.3848469344366643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377530.3741919829964816No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC283180.2806761998912501No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA254860.2526065975855781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231080.22903685384162045No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG207280.2054472869321927No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTT193840.19212611973628055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188240.18657563340465047No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG178970.1773875962092557No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG166790.16531528843796034No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTA152670.15132013361606453No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTCATCTCAT151120.1497838382921312No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGCAGAAATG147510.14620575692477683No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG134360.13317202562818123No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATC127640.12651144203022516No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTC124700.12359743670611939No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC123800.12270539425996456No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA121120.12004909008697018No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT113170.1121693818126025No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCTGGATTAT113100.11210000073345712No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTATAAAGTCC111770.11078176022969498No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC110080.10910670274747092No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGCAATGAAT107210.1062620785025105No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC107110.10616296267515997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG13700.017.66208624
AGGTCGT14600.017.47628822
CGTCATT14800.017.24106226
CATTGCG15250.017.02105129
GTACTAG17500.016.6030581
GTCGTCA15300.016.5339424
TCGTCAG16050.016.31278241
GCTCGTC16100.016.12554439
CAATGCG14250.016.05339219
GTCGACG13750.015.83886136
AATGCGA14750.015.6582620
TAGGTCG16350.015.47158821
ATCGTCA14700.015.41446910
TGCGACG15000.015.39721222
CGACGAA13900.015.3514938
AGGTCGC28750.015.15081710
TCGACGA14150.015.08018837
GGTCGCC29050.015.06963411
GTGATCG16450.014.9786128
GAGGTCG30600.014.7380889
Produced by FastQC (version 0.11.5)