##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558153_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10089206
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.867926970665483	33.0	32.0	34.0	27.0	34.0
2	31.929466798477502	33.0	32.0	34.0	27.0	34.0
3	31.92866376204431	33.0	32.0	34.0	27.0	34.0
4	31.8291707989707	33.0	32.0	34.0	28.0	34.0
5	31.88875041306521	33.0	32.0	34.0	28.0	34.0
6	35.356436770148214	38.0	36.0	38.0	29.0	38.0
7	35.40584838886232	38.0	37.0	38.0	29.0	38.0
8	35.45001341037144	38.0	37.0	38.0	29.0	38.0
9	35.60479228989873	38.0	37.0	38.0	29.0	38.0
10	35.565793978237735	38.0	37.0	38.0	29.0	38.0
11	35.61232172283924	38.0	37.0	38.0	29.0	38.0
12	35.5198818420399	38.0	37.0	38.0	29.0	38.0
13	35.57148768693988	38.0	37.0	38.0	29.0	38.0
14	35.576821506072925	38.0	37.0	38.0	29.0	38.0
15	35.566830432444334	38.0	37.0	38.0	29.0	38.0
16	35.5178789094008	38.0	37.0	38.0	29.0	38.0
17	35.49136730878525	38.0	37.0	38.0	29.0	38.0
18	35.49471167503171	38.0	37.0	38.0	28.0	38.0
19	35.50916791668244	38.0	37.0	38.0	28.0	38.0
20	35.440999717916355	38.0	37.0	38.0	28.0	38.0
21	35.45211436856379	38.0	37.0	38.0	28.0	38.0
22	35.496844647636294	38.0	37.0	38.0	28.0	38.0
23	35.49877433367898	38.0	37.0	38.0	28.0	38.0
24	35.495187431003	38.0	37.0	38.0	28.0	38.0
25	35.50348144343569	38.0	37.0	38.0	28.0	38.0
26	35.50594913018924	38.0	37.0	38.0	28.0	38.0
27	35.5167413570503	38.0	37.0	38.0	28.0	38.0
28	35.52586863624352	38.0	37.0	38.0	29.0	38.0
29	35.49279388288831	38.0	37.0	38.0	28.0	38.0
30	35.49955239292368	38.0	37.0	38.0	28.0	38.0
31	35.53822045064795	38.0	37.0	38.0	29.0	38.0
32	35.54899077291117	38.0	37.0	38.0	29.0	38.0
33	35.526865939698325	38.0	37.0	38.0	29.0	38.0
34	35.504305988003416	38.0	37.0	38.0	28.0	38.0
35	35.516181451741595	38.0	37.0	38.0	29.0	38.0
36	35.4943959911216	38.0	37.0	38.0	28.0	38.0
37	35.49131834556654	38.0	37.0	38.0	28.0	38.0
38	35.490723848834094	38.0	37.0	38.0	28.0	38.0
39	35.5045277101092	38.0	37.0	38.0	28.0	38.0
40	35.51213197549936	38.0	37.0	38.0	28.0	38.0
41	35.51057199149269	38.0	37.0	38.0	28.0	38.0
42	35.425228803931645	38.0	37.0	38.0	28.0	38.0
43	35.46683386185196	38.0	37.0	38.0	28.0	38.0
44	35.45717115895939	38.0	37.0	38.0	28.0	38.0
45	35.479754501989554	38.0	37.0	38.0	28.0	38.0
46	35.44657220796166	38.0	37.0	38.0	28.0	38.0
47	35.403877767982934	38.0	37.0	38.0	28.0	38.0
48	35.37943203855685	38.0	37.0	38.0	28.0	38.0
49	35.29866998453595	38.0	37.0	38.0	27.0	38.0
50	35.014301224496755	38.0	36.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	14.0
4	484.0
5	1408.0
6	2124.0
7	1066.0
8	266.0
9	159.0
10	187.0
11	155.0
12	137.0
13	125.0
14	134.0
15	191.0
16	496.0
17	1654.0
18	8097.0
19	26486.0
20	49032.0
21	66766.0
22	79892.0
23	91856.0
24	104317.0
25	117062.0
26	131334.0
27	147793.0
28	167179.0
29	189685.0
30	216647.0
31	247506.0
32	291158.0
33	354990.0
34	456475.0
35	659643.0
36	1323554.0
37	5351134.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.10641288476937	19.817715318388363	13.948650316938158	24.12722147990411
2	14.773055481273751	21.552558248884996	39.60974728833963	24.064638981501616
3	17.513340494782245	27.301851107014762	30.908874295955503	24.27593410224749
4	12.496309835436008	17.889352757526893	37.54407627721329	32.070261129823805
5	13.220472725848847	38.09002380630206	34.70904317302369	13.980460294825415
6	29.98683826840411	37.04116934100093	18.956196328997667	14.0157960615973
7	25.53483854765293	32.58470351636781	23.96335171176504	17.917106224214223
8	25.999598273070163	33.63454256445609	21.39458759754897	18.971271564924773
9	26.384722024141183	15.420901661374845	20.648211310012027	37.54616500447195
10	16.3705881326034	28.161410131176655	32.26373561845694	23.204266117763005
11	32.51992273415939	23.843226716722498	23.830362301411167	19.806488247706948
12	22.517751071411922	26.27190307156268	29.88225130895245	21.328094548072947
13	27.7158397921616	21.676260472090018	26.82751553585469	23.78038419989369
14	22.867023074106914	21.38891977480054	26.840795099577885	28.903262051514663
15	24.645484188221868	27.421883337479137	25.091118418834093	22.841514055464902
16	23.32555025806543	27.658482584984746	25.870478003017816	23.14548915393201
17	22.4546560045706	27.32862717247481	26.980565017030656	23.236151805923928
18	22.467895099389885	26.25966400759312	29.22429822361613	22.048142669400868
19	23.249525864180843	26.95662230026241	29.0656910937447	20.728160741812047
20	24.329519054072495	24.966684731034	29.082732157536096	21.621064057357408
21	23.736992092429972	26.38608607269323	27.211595957676465	22.665325877200335
22	23.320045487492578	26.444985055512138	27.096544693980384	23.138424763014903
23	21.838114384030945	26.941276961374243	27.564456342485062	23.65615231210975
24	23.136035402548277	27.601987128690286	27.114616940182824	22.14736052857861
25	23.40868942177143	25.50073162165703	27.20222185260112	23.888357103970414
26	23.079785332367397	25.987675900072343	27.537307253090322	23.395231514469934
27	23.393584682721638	25.582960243868257	27.328169007519378	23.695286065890723
28	22.220721500652814	26.233568338397774	28.271293701987236	23.274416458962175
29	22.80062893936357	25.985137821252565	28.5919155325667	22.622317706817178
30	22.399500242133442	27.08722942716177	28.108662625059715	22.404607705645077
31	24.05849877101922	25.85425668481443	27.18084583074816	22.906398713418184
32	21.97447930865585	26.550485979249004	27.824457803569558	23.650576908525583
33	22.881136985501158	26.41306213870296	27.38036900535885	23.325431870437033
34	21.953759937081063	26.684591555036963	28.015678161307132	23.34597034657484
35	23.233196018310654	26.728495619457377	27.831270632586357	22.207037729645617
36	23.03495855476105	26.767241610143216	27.059800904970192	23.137998930125534
37	23.928632457313846	26.281873845775756	27.20500530046848	22.58448839644192
38	23.60555900779755	26.31500746626542	27.222474872334544	22.85695865360248
39	23.687544086410554	25.59303908803317	27.585328280604198	23.13408854495207
40	23.397086365311537	26.237876219518725	27.23973319252974	23.125304222639993
41	22.08256953624254	26.983947341718313	27.725375646328317	23.208107475710833
42	24.252624053869287	26.550423558906438	27.102979665225163	22.09397272199911
43	23.59390265719464	25.258412743998633	27.120981750256945	24.026702848549782
44	22.74482500066947	26.172132017404227	27.706476795194895	23.376566186731413
45	24.272086616631988	25.70097951261292	27.171497481579	22.85543638917609
46	22.588041734000807	26.505384567118828	28.36412902819078	22.542444670689584
47	23.425114522030924	26.085398030908035	27.454596875809457	23.03489057125158
48	23.941576055033476	26.735719900383632	27.246530889908165	22.07617315467473
49	23.016438463277257	26.496387173827056	28.735398710778025	21.751775652117654
50	23.613962754054498	26.62298147377357	26.792049633241245	22.97100613893069
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	747.0
1	1020.5
2	1294.0
3	3943.5
4	6593.0
5	7514.0
6	8435.0
7	7415.0
8	6395.0
9	8206.0
10	10017.0
11	13598.0
12	17179.0
13	31654.5
14	46130.0
15	59371.5
16	72613.0
17	69347.0
18	66081.0
19	63924.5
20	61768.0
21	71346.0
22	80924.0
23	95889.0
24	110854.0
25	124716.0
26	138578.0
27	145374.0
28	152170.0
29	176598.0
30	201026.0
31	261899.5
32	322773.0
33	341332.0
34	359891.0
35	387883.0
36	415875.0
37	441011.5
38	466148.0
39	501094.5
40	536041.0
41	571514.0
42	606987.0
43	600909.0
44	594831.0
45	641476.5
46	688122.0
47	753872.5
48	819623.0
49	833525.0
50	847427.0
51	796585.0
52	745743.0
53	627574.0
54	509405.0
55	492058.0
56	474711.0
57	448218.5
58	421726.0
59	390951.5
60	360177.0
61	310677.5
62	261178.0
63	230183.0
64	199188.0
65	168604.0
66	138020.0
67	123563.5
68	109107.0
69	92944.5
70	76782.0
71	62495.5
72	48209.0
73	44401.5
74	40594.0
75	31342.5
76	22091.0
77	18549.5
78	15008.0
79	12234.5
80	9461.0
81	8120.0
82	6779.0
83	6391.0
84	6003.0
85	4679.5
86	3356.0
87	3140.5
88	2925.0
89	1529.0
90	133.0
91	103.5
92	74.0
93	41.0
94	8.0
95	5.0
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04391822309902286
2	0.0
3	0.0
4	0.015660300721384815
5	0.015273748994717722
6	0.06884585367768285
7	0.07035241425341103
8	0.0766264461247
9	0.06295837353306097
10	0.07467386432589443
11	0.0706794964836678
12	0.06656618964862052
13	0.07460448324674905
14	0.07234464238315681
15	0.06662565914503084
16	0.07010462468503467
17	0.07320695008110649
18	0.0842682764134264
19	0.06593184835357707
20	0.0668238907997319
21	0.0653074186412687
22	0.0550588420932232
23	0.057021335474763825
24	0.06260155655459904
25	0.0674879668429805
26	0.05660504899989157
27	0.060034456626220145
28	0.051223059574757424
29	0.0677456679940919
30	0.05870630453972295
31	0.060004721878014984
32	0.05495972626587266
33	0.0620762426696412
34	0.06334492525972807
35	0.06493077849733665
36	0.057288948208610274
37	0.06080756007955433
38	0.07127419144777102
39	0.06674459813785148
40	0.06075800216587906
41	0.07383137979341486
42	0.06372156540366011
43	0.052908028639716546
44	0.06537679972041407
45	0.015224191081042454
46	0.008295994749239931
47	0.013965420073690636
48	0.020903527988228213
49	0.11398320145311733
50	0.009584500504796908
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0089206E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.532277058946555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.50170352405502	21.220452680652098
2	14.783961436185322	9.027760131967835
3	6.055022753709598	5.546208969434609
4	3.061664297099811	3.7391833032214516
5	1.7594504245053182	2.686001391623875
6	1.078131689891715	1.9750689277082456
7	0.6970500588987928	1.4897767865577072
8	0.4962780722051342	1.2121999679077633
9	0.3468540710169598	0.9531220135782007
>10	1.8470416615349594	10.33798154901052
>50	0.16971119121212086	3.6330980120554828
>100	0.15130708093793332	9.849200694029477
>500	0.02542305201805929	5.422833269156744
>1k	0.02375735469657501	14.584103878568419
>5k	0.0018274888127534736	3.6524576052568993
>10k+	8.158432199792293E-4	4.670550819270912
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	47210	0.46792582092188423	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	38828	0.3848469344366643	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37753	0.3741919829964816	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	28318	0.2806761998912501	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	25486	0.2526065975855781	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23108	0.22903685384162045	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	20728	0.2054472869321927	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTT	19384	0.19212611973628055	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18824	0.18657563340465047	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	17897	0.1773875962092557	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	16679	0.16531528843796034	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTA	15267	0.15132013361606453	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTCATCTCAT	15112	0.1497838382921312	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGCAGAAATG	14751	0.14620575692477683	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	13436	0.13317202562818123	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATC	12764	0.12651144203022516	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTC	12470	0.12359743670611939	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	12380	0.12270539425996456	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	12112	0.12004909008697018	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	11317	0.1121693818126025	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCTGGATTAT	11310	0.11210000073345712	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTATAAAGTCC	11177	0.11078176022969498	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	11008	0.10910670274747092	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGCAATGAAT	10721	0.1062620785025105	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	10711	0.10616296267515997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.973474820516104E-5	0.0	0.0	0.0	0.0
2	3.964633094021472E-5	0.0	0.0	9.91158273505368E-6	0.0
3	3.964633094021472E-5	0.0	0.0	9.91158273505368E-6	0.0
4	3.964633094021472E-5	0.0	0.0	9.91158273505368E-6	0.0
5	4.95579136752684E-5	9.91158273505368E-6	0.0	9.91158273505368E-6	0.0
6	6.938107914537576E-5	9.91158273505368E-6	0.0	9.91158273505368E-6	0.0
7	6.938107914537576E-5	9.91158273505368E-6	0.0	9.91158273505368E-6	0.0
8	6.938107914537576E-5	9.91158273505368E-6	0.0	9.91158273505368E-6	0.0
9	6.938107914537576E-5	9.91158273505368E-6	0.0	2.973474820516104E-5	0.0
10	1.486737410258052E-4	9.91158273505368E-6	0.0	7.929266188042944E-5	0.0
11	2.1805482017118097E-4	9.91158273505368E-6	0.0	7.929266188042944E-5	0.0
12	2.2796640290623465E-4	9.91158273505368E-6	0.0	9.91158273505368E-5	0.0
13	2.2796640290623465E-4	9.91158273505368E-6	0.0	1.0902741008559048E-4	0.0
14	2.378779856412883E-4	9.91158273505368E-6	0.0	1.1893899282064416E-4	0.0
15	2.378779856412883E-4	9.91158273505368E-6	0.0	1.1893899282064416E-4	0.0
16	2.47789568376342E-4	9.91158273505368E-6	0.0	1.7840848923096625E-4	0.0
17	2.47789568376342E-4	9.91158273505368E-6	0.0	2.0814323743612728E-4	0.0
18	2.47789568376342E-4	9.91158273505368E-6	0.0	2.1805482017118097E-4	0.0
19	2.577011511113957E-4	9.91158273505368E-6	0.0	2.378779856412883E-4	0.0
20	2.7752431658150306E-4	9.91158273505368E-6	0.0	2.6761273384644935E-4	0.0
21	3.072590647866641E-4	9.91158273505368E-6	0.0	3.6672856119698616E-4	0.0
22	3.6672856119698616E-4	9.91158273505368E-6	0.0	5.451370504279524E-4	0.0
23	3.8655172666709353E-4	9.91158273505368E-6	0.0	0.0010109814389754753	0.0
24	4.261980576073082E-4	9.91158273505368E-6	0.0	0.0014470910793178372	0.0
25	4.261980576073082E-4	9.91158273505368E-6	0.0	0.001516472158463213	0.0
26	4.757559712825766E-4	9.91158273505368E-6	0.0	0.0017246153958993403	0.0
27	5.054907194877377E-4	9.91158273505368E-6	0.0	0.0019624933815406286	0.0
28	5.253138849578451E-4	1.982316547010736E-5	0.0	0.003032944316926426	0.0
29	5.550486331630061E-4	1.982316547010736E-5	0.0	0.004440389065304049	0.0
30	5.550486331630061E-4	1.982316547010736E-5	0.0	0.006769611008041664	0.0
31	5.748717986331134E-4	1.982316547010736E-5	0.0	0.010526100864627009	0.0
32	5.748717986331134E-4	1.982316547010736E-5	9.91158273505368E-6	0.013806834749929777	0.0
33	5.847833813681672E-4	1.982316547010736E-5	9.91158273505368E-6	0.017355181369078994	0.0
34	6.046065468382745E-4	1.982316547010736E-5	9.91158273505368E-6	0.02214247583010992	0.0
35	6.046065468382745E-4	1.982316547010736E-5	9.91158273505368E-6	0.02870394360071546	0.0
36	6.145181295733282E-4	1.982316547010736E-5	9.91158273505368E-6	0.036276392810296466	0.0
37	6.442528777784892E-4	1.982316547010736E-5	9.91158273505368E-6	0.04706019482603487	9.91158273505368E-6
38	6.541644605135429E-4	2.973474820516104E-5	9.91158273505368E-6	0.059806490223313906	9.91158273505368E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAG	1370	0.0	17.662086	24
AGGTCGT	1460	0.0	17.476288	22
CGTCATT	1480	0.0	17.241062	26
CATTGCG	1525	0.0	17.021051	29
GTACTAG	1750	0.0	16.603058	1
GTCGTCA	1530	0.0	16.53394	24
TCGTCAG	1605	0.0	16.312782	41
GCTCGTC	1610	0.0	16.125544	39
CAATGCG	1425	0.0	16.053392	19
GTCGACG	1375	0.0	15.838861	36
AATGCGA	1475	0.0	15.65826	20
TAGGTCG	1635	0.0	15.471588	21
ATCGTCA	1470	0.0	15.414469	10
TGCGACG	1500	0.0	15.397212	22
CGACGAA	1390	0.0	15.35149	38
AGGTCGC	2875	0.0	15.150817	10
TCGACGA	1415	0.0	15.080188	37
GGTCGCC	2905	0.0	15.069634	11
GTGATCG	1645	0.0	14.978612	8
GAGGTCG	3060	0.0	14.738088	9
>>END_MODULE