FastQCFastQC Report
Wed 25 May 2016
SRR2558152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10414213
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA750880.7210146364396426No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT617690.5931221110995137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514850.4943724504194412No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC442140.4245544046391216No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA389930.374420995614359No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG331000.31783486663850646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312310.2998882392745376No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG282090.2708702040182969No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG265830.255256926279499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255050.2449056880246256No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTT212410.20396164357306693No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA191320.18371047336942312No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC190820.18323036027782416No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT179820.1726678722626472No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC172200.1653509487466792No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC164190.15765953701926397No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC147050.14120126023925186No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT129650.12449332465160833No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG114760.11019555678379153No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG113270.10876481977082665No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC111610.10717084430671815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGA12300.019.85052120
CAATGCG12350.019.41412419
GTACTAG15500.019.1679341
CGACGAG13600.017.46841624
TGCGACG13800.017.21442222
CGACGAA11150.016.77018238
GATCACT65000.015.805099538
CGAACAA12100.015.45581541
CGTCGTA21650.015.44521710
ATCGTCA15550.015.420711510
CCGTCGT21900.015.2687559
CATTGCG12850.015.23567929
GTCGTCA13600.014.71875824
AATTCGT12150.014.66674416
CAATTCG12250.014.54701715
ACTGTCC70400.014.50028642
GACGAGT15950.014.48088725
GCAGATC73400.014.41600235
GTATTAG21500.014.3305461
AGTCGAC12950.014.26938635
Produced by FastQC (version 0.11.5)