##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10414213
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.887634620110035	33.0	32.0	34.0	27.0	34.0
2	31.94778626094934	33.0	32.0	34.0	27.0	34.0
3	31.942311627388456	33.0	32.0	34.0	27.0	34.0
4	31.849917511769732	33.0	32.0	34.0	28.0	34.0
5	31.89922599048051	33.0	32.0	34.0	28.0	34.0
6	35.378973908061994	38.0	36.0	38.0	29.0	38.0
7	35.43498927859455	38.0	37.0	38.0	29.0	38.0
8	35.485415364559955	38.0	37.0	38.0	29.0	38.0
9	35.64118709690305	38.0	37.0	38.0	29.0	38.0
10	35.58408503839897	38.0	37.0	38.0	29.0	38.0
11	35.633440280124866	38.0	37.0	38.0	29.0	38.0
12	35.54125318927124	38.0	37.0	38.0	29.0	38.0
13	35.591977425466524	38.0	37.0	38.0	29.0	38.0
14	35.61132886373651	38.0	37.0	38.0	29.0	38.0
15	35.59940573521974	38.0	37.0	38.0	29.0	38.0
16	35.559918353888094	38.0	37.0	38.0	29.0	38.0
17	35.52449320942446	38.0	37.0	38.0	29.0	38.0
18	35.52940428623843	38.0	37.0	38.0	29.0	38.0
19	35.54187992890101	38.0	37.0	38.0	29.0	38.0
20	35.46599968715831	38.0	37.0	38.0	28.0	38.0
21	35.48238402652222	38.0	37.0	38.0	28.0	38.0
22	35.518767092626206	38.0	37.0	38.0	28.0	38.0
23	35.5228371073263	38.0	37.0	38.0	29.0	38.0
24	35.52372608472671	38.0	37.0	38.0	29.0	38.0
25	35.5339715060562	38.0	37.0	38.0	29.0	38.0
26	35.53813706326152	38.0	37.0	38.0	29.0	38.0
27	35.53672370634248	38.0	37.0	38.0	29.0	38.0
28	35.546984779358745	38.0	37.0	38.0	29.0	38.0
29	35.51363958083054	38.0	37.0	38.0	29.0	38.0
30	35.51957339455223	38.0	37.0	38.0	29.0	38.0
31	35.560096187777226	38.0	37.0	38.0	29.0	38.0
32	35.56870874448218	38.0	37.0	38.0	29.0	38.0
33	35.53565631891723	38.0	37.0	38.0	29.0	38.0
34	35.512437761739655	38.0	37.0	38.0	29.0	38.0
35	35.51600644234951	38.0	37.0	38.0	29.0	38.0
36	35.49502588433711	38.0	37.0	38.0	28.0	38.0
37	35.4803733128946	38.0	37.0	38.0	28.0	38.0
38	35.48201606784881	38.0	37.0	38.0	28.0	38.0
39	35.48465707394308	38.0	37.0	38.0	28.0	38.0
40	35.48574654657054	38.0	37.0	38.0	28.0	38.0
41	35.47834838791947	38.0	37.0	38.0	28.0	38.0
42	35.38698219442986	38.0	37.0	38.0	28.0	38.0
43	35.435590284162615	38.0	37.0	38.0	28.0	38.0
44	35.41231152080335	38.0	37.0	38.0	28.0	38.0
45	35.42350449333041	38.0	37.0	38.0	28.0	38.0
46	35.38553859038604	38.0	37.0	38.0	28.0	38.0
47	35.32678782352541	38.0	37.0	38.0	27.0	38.0
48	35.28676194735022	38.0	37.0	38.0	27.0	38.0
49	35.19923761881959	38.0	37.0	38.0	27.0	38.0
50	34.90399476177412	38.0	36.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	20.0
4	525.0
5	1503.0
6	2183.0
7	1070.0
8	272.0
9	184.0
10	152.0
11	140.0
12	108.0
13	131.0
14	128.0
15	210.0
16	511.0
17	1684.0
18	8251.0
19	25405.0
20	46844.0
21	64630.0
22	78690.0
23	92129.0
24	105793.0
25	120712.0
26	135326.0
27	153312.0
28	172943.0
29	198091.0
30	227943.0
31	262068.0
32	307508.0
33	374284.0
34	483679.0
35	696330.0
36	1391517.0
37	5459937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73744575804913	19.047545397718586	13.753974813078953	24.461034031153332
2	14.654232633805359	21.149365775407126	39.40524358393668	24.79115800685083
3	17.760842802043708	27.554516121381422	29.8921195485439	24.79252152803097
4	12.3278138428878	17.625468247523767	37.5617947475605	32.484923162027926
5	13.331751276913526	37.712895750997255	34.40776796744869	14.547585004640528
6	30.474890264808668	36.672650082410094	18.783659115763193	14.068800537018051
7	26.205220742254838	32.07215858769235	23.78749083181359	17.935129838239224
8	26.919155126342726	32.74590280902876	21.28983522251874	19.045106842109767
9	27.2679624393117	14.593392001491203	20.518923987935842	37.61972157126125
10	16.635110980465903	28.06221244454532	31.816741717725446	23.48593485726333
11	32.82589795162983	23.808551012462107	23.0740388980089	20.291512137899172
12	23.45888870952864	25.804113627279158	29.921392168619132	20.815605494573074
13	28.723595420105536	21.270062406981463	25.994702555934126	24.01163961697888
14	23.37997260048355	20.754437046790674	27.100162750091215	28.765427602634556
15	25.15608934116793	26.612215358370594	25.012911559254185	23.21878374120729
16	22.92238957532831	26.765042081275265	26.738041034672417	23.574527308724008
17	22.299942690590303	26.976136838419368	27.49053779749676	23.233382673493573
18	23.42268195794626	25.1904882948865	29.481951603455673	21.904878143711567
19	23.272016415235115	26.41200183907516	29.100991073182986	21.21499067250674
20	24.16011308991559	24.861861306118573	28.951291277060108	22.026734326905725
21	24.625225487338025	25.57625502788481	27.448537385401895	22.349982099375275
22	23.867288544451657	25.539464182844828	27.247610505130627	23.345636767572888
23	22.239174563596773	26.21827232668195	27.750602790244365	23.791950319476907
24	22.867520342638755	27.20995117459234	27.33683804753713	22.585690435231776
25	23.372606573721256	25.06336924930068	27.15713921133486	24.4068849656432
26	23.395143138758034	25.433309310900782	27.97553343587303	23.196014114468156
27	23.934257442713637	25.21787471496496	27.019001182847962	23.828866659473434
28	22.857454130214172	24.973419297220342	28.758057913507784	23.411068659057705
29	22.975905714487226	24.590691942249972	29.371392761048654	23.062009582214156
30	23.17443169600831	25.684521459013453	28.827677435806088	22.31336940917215
31	24.266057214274515	24.70322919837295	27.626692538992963	23.404021048359574
32	22.016625874587774	25.533529167337193	28.38038233266641	24.069462625408622
33	23.102908031074392	26.146116594499624	27.619108287843403	23.13186708658258
34	22.189353120643514	25.8012956477148	28.410011017896224	23.599340213745457
35	23.116027314561656	26.259773130675416	28.71721507464481	21.906984480118115
36	23.655587933779326	25.74857588658206	27.38439677063247	23.211439409006143
37	23.739666765373528	26.14962909983376	27.75332097992279	22.357383154869915
38	23.96057413969131	25.48638279983184	27.49461293615999	23.05843012431686
39	23.65250600493001	25.072502119189238	27.699009212972225	23.57598266290853
40	23.561419085651465	25.686388808207756	27.760723979917973	22.99146812622281
41	22.116236053680936	25.920725877878066	27.942577589469913	24.020460478971085
42	24.273076955967085	26.424446901836458	27.26612021348628	22.03635592871018
43	24.2227075658036	24.31570702212186	27.370700732649333	24.090884679425205
44	23.206399878241044	25.774637548032906	28.191656590549385	22.827305983176664
45	24.84130667111259	24.81147724243162	27.284765209935408	23.06245087652038
46	22.690574364193537	25.42749535664634	29.184086345203152	22.697843933956968
47	23.820354940799984	25.621997286765946	27.855498294096826	22.702149478337248
48	24.335498092861517	26.176539344179478	26.98570697816563	22.502255584793375
49	23.137537386724667	25.816009247374183	28.795093831161587	22.251359534739564
50	23.50225303412725	26.277744470984242	26.892574115138046	23.327428379750454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1413.0
1	1547.5
2	1682.0
3	4847.0
4	8012.0
5	9439.5
6	10867.0
7	9728.5
8	8590.0
9	10856.5
10	13123.0
11	18247.0
12	23371.0
13	41548.0
14	59725.0
15	74100.5
16	88476.0
17	81634.5
18	74793.0
19	64909.5
20	55026.0
21	63328.5
22	71631.0
23	85388.0
24	99145.0
25	122674.0
26	146203.0
27	166286.5
28	186370.0
29	214587.0
30	242804.0
31	271857.5
32	300911.0
33	327227.0
34	353543.0
35	386602.5
36	419662.0
37	444115.5
38	468569.0
39	488457.0
40	508345.0
41	528363.5
42	548382.0
43	558474.0
44	568566.0
45	620587.5
46	672609.0
47	764944.0
48	857279.0
49	866427.5
50	875576.0
51	758345.5
52	641115.0
53	579572.0
54	518029.0
55	525742.5
56	533456.0
57	499091.5
58	464727.0
59	441649.0
60	418571.0
61	364160.0
62	309749.0
63	270814.0
64	231879.0
65	196448.0
66	161017.0
67	143959.0
68	126901.0
69	116369.5
70	105838.0
71	87309.5
72	68781.0
73	65464.5
74	62148.0
75	56101.0
76	50054.0
77	37936.5
78	25819.0
79	18306.5
80	10794.0
81	9259.5
82	7725.0
83	6957.0
84	6189.0
85	4820.5
86	3452.0
87	3269.5
88	3087.0
89	1609.5
90	132.0
91	100.5
92	69.0
93	37.0
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04420881347443153
2	0.0
3	0.0
4	0.016496685827340003
5	0.015411630240326369
6	0.06831049067269893
7	0.07011571589711099
8	0.07562741418866696
9	0.06263555392999932
10	0.07434071110318179
11	0.07014452268260693
12	0.0658043003345524
13	0.07379338217875897
14	0.07163287326656369
15	0.06598674330936001
16	0.06922270554673694
17	0.0724490655222819
18	0.08371251865119332
19	0.06537219855211335
20	0.06643804961546301
21	0.06486327867501845
22	0.05508817613006379
23	0.05712385563844335
24	0.06257794035900745
25	0.06680293556507823
26	0.05651891314302867
27	0.060321408828492365
28	0.05133369175375998
29	0.067359866751333
30	0.05894828538651936
31	0.05935158038346248
32	0.055097778391895766
33	0.06159850965214558
34	0.06337492809106171
35	0.06456560855822711
36	0.057450332540730634
37	0.06040782918498018
38	0.07046139732306224
39	0.06655327675744677
40	0.06049424954146799
41	0.07311162158868846
42	0.06349015523304546
43	0.0532925531674837
44	0.06495930129333825
45	0.016102993092228863
46	0.009045330645724263
47	0.014854699054071585
48	0.021883554715080248
49	0.11642742471274595
50	0.00885328540908468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0414213E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.22186047629004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.33622024057176	24.773725357430262
2	15.128103499834195	10.6567990148407
3	5.997232208981065	6.337010283259311
4	2.8135228649905577	3.96390039190197
5	1.5136295773398616	2.6656424892925235
6	0.9259846389649065	1.9568941054085847
7	0.6171549228153923	1.5216141208561509
8	0.4327194093801743	1.2192946130056903
9	0.3053181342546829	0.9678485453039669
>10	1.61205969240859	10.304417301439955
>50	0.14696433415065857	3.6310131806700507
>100	0.13640048913289538	10.135635552622967
>500	0.01951136340374693	4.756533091767184
>1k	0.013671720778812586	9.238280380166065
>5k	9.31540031826599E-4	2.188532772219085
>10k+	5.753629608340759E-4	5.6828587998156
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	75088	0.7210146364396426	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	61769	0.5931221110995137	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51485	0.4943724504194412	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	44214	0.4245544046391216	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	38993	0.374420995614359	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	33100	0.31783486663850646	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31231	0.2998882392745376	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	28209	0.2708702040182969	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	26583	0.255256926279499	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25505	0.2449056880246256	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTT	21241	0.20396164357306693	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	19132	0.18371047336942312	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	19082	0.18323036027782416	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	17982	0.1726678722626472	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	17220	0.1653509487466792	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	16419	0.15765953701926397	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	14705	0.14120126023925186	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	12965	0.12449332465160833	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	11476	0.11019555678379153	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	11327	0.10876481977082665	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	11161	0.10717084430671815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9204523663958094E-5	0.0	0.0	0.0	0.0
2	2.880678549593714E-5	0.0	0.0	0.0	0.0
3	2.880678549593714E-5	0.0	0.0	9.602261831979047E-6	0.0
4	2.880678549593714E-5	0.0	0.0	3.840904732791619E-5	0.0
5	1.248294038157276E-4	0.0	0.0	5.761357099187428E-5	0.0
6	1.440339274796857E-4	0.0	0.0	5.761357099187428E-5	0.0
7	1.7284071297562283E-4	0.0	0.0	5.761357099187428E-5	0.0
8	1.9204523663958093E-4	0.0	0.0	5.761357099187428E-5	2.880678549593714E-5
9	2.0164749847155998E-4	0.0	0.0	8.642035648781141E-5	2.880678549593714E-5
10	3.8409047327916186E-4	0.0	0.0	1.440339274796857E-4	2.880678549593714E-5
11	5.089198770948895E-4	1.9204523663958094E-5	0.0	1.7284071297562283E-4	2.880678549593714E-5
12	5.185221389268685E-4	1.9204523663958094E-5	0.0	1.9204523663958093E-4	2.880678549593714E-5
13	5.185221389268685E-4	1.9204523663958094E-5	0.0	2.0164749847155998E-4	2.880678549593714E-5
14	5.185221389268685E-4	1.9204523663958094E-5	0.0	2.11249760303539E-4	2.880678549593714E-5
15	6.049424954146799E-4	1.9204523663958094E-5	0.0	2.2085202213551806E-4	3.840904732791619E-5
16	6.913628519024913E-4	1.9204523663958094E-5	0.0	2.496588076314552E-4	5.761357099187428E-5
17	8.642035648781141E-4	1.9204523663958094E-5	0.0	3.1687464045530855E-4	5.761357099187428E-5
18	8.834080885420722E-4	1.9204523663958094E-5	0.0	3.360791641192666E-4	5.761357099187428E-5
19	9.602261831979047E-4	1.9204523663958094E-5	0.0	3.6488594961520376E-4	5.761357099187428E-5
20	9.698284450298837E-4	1.9204523663958094E-5	0.0	4.705108297669733E-4	5.761357099187428E-5
21	0.0010274420160217579	1.9204523663958094E-5	0.0	5.665334480867637E-4	9.602261831979047E-5
22	0.001065851063349674	1.9204523663958094E-5	0.0	8.546013030461351E-4	9.602261831979047E-5
23	0.0011618736816694647	1.9204523663958094E-5	0.0	0.0013059076091491502	1.056248801517695E-4
24	0.0011810782053334227	1.9204523663958094E-5	0.0	0.001670793558764354	1.056248801517695E-4
25	0.0012002827289973807	2.880678549593714E-5	0.0	0.001843634271739977	1.056248801517695E-4
26	0.0013251121328131084	2.880678549593714E-5	0.0	0.002035679508379558	1.056248801517695E-4
27	0.0013827257038049826	2.880678549593714E-5	0.0	0.0022373270068511177	1.056248801517695E-4
28	0.0014691460602927941	2.880678549593714E-5	0.0	0.003504825568672352	1.248294038157276E-4
29	0.0015459641549486266	4.801130915989523E-5	0.0	0.004964369367133167	1.248294038157276E-4
30	0.0016515890351003959	4.801130915989523E-5	0.0	0.007393741610623865	1.248294038157276E-4
31	0.0017188048679242494	4.801130915989523E-5	0.0	0.010466465396857161	1.248294038157276E-4
32	0.0017380093915882074	4.801130915989523E-5	0.0	0.014038506798353365	1.248294038157276E-4
33	0.0017764184389161236	4.801130915989523E-5	0.0	0.01861878569220737	1.248294038157276E-4
34	0.001843634271739977	4.801130915989523E-5	0.0	0.023544746012012622	1.440339274796857E-4
35	0.001853236533571956	4.801130915989523E-5	0.0	0.030928885360804508	1.440339274796857E-4
36	0.0018916455808998722	6.721583282385332E-5	0.0	0.039494102914929816	1.440339274796857E-4
37	0.0019588614137237255	6.721583282385332E-5	0.0	0.05260119031558121	1.440339274796857E-4
38	0.0019780659373876837	7.681809465583238E-5	0.0	0.06601555009485595	1.440339274796857E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGA	1230	0.0	19.850521	20
CAATGCG	1235	0.0	19.414124	19
GTACTAG	1550	0.0	19.167934	1
CGACGAG	1360	0.0	17.468416	24
TGCGACG	1380	0.0	17.214422	22
CGACGAA	1115	0.0	16.770182	38
GATCACT	6500	0.0	15.8050995	38
CGAACAA	1210	0.0	15.455815	41
CGTCGTA	2165	0.0	15.445217	10
ATCGTCA	1555	0.0	15.4207115	10
CCGTCGT	2190	0.0	15.268755	9
CATTGCG	1285	0.0	15.235679	29
GTCGTCA	1360	0.0	14.718758	24
AATTCGT	1215	0.0	14.666744	16
CAATTCG	1225	0.0	14.547017	15
ACTGTCC	7040	0.0	14.500286	42
GACGAGT	1595	0.0	14.480887	25
GCAGATC	7340	0.0	14.416002	35
GTATTAG	2150	0.0	14.330546	1
AGTCGAC	1295	0.0	14.269386	35
>>END_MODULE