##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2558151_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11593259 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.973883098790427 33.0 32.0 34.0 28.0 34.0 2 32.01458890895131 33.0 32.0 34.0 28.0 34.0 3 32.0118771606845 33.0 32.0 34.0 28.0 34.0 4 31.91540359790116 33.0 33.0 34.0 28.0 34.0 5 31.965180541554364 33.0 33.0 34.0 28.0 34.0 6 35.45759721231105 38.0 36.0 38.0 29.0 38.0 7 35.53337909555889 38.0 37.0 38.0 29.0 38.0 8 35.590167786297194 38.0 37.0 38.0 29.0 38.0 9 35.735851584097276 38.0 37.0 38.0 29.0 38.0 10 35.67079696916976 38.0 37.0 38.0 29.0 38.0 11 35.71954081246697 38.0 37.0 38.0 29.0 38.0 12 35.63739523114251 38.0 37.0 38.0 29.0 38.0 13 35.68597777380804 38.0 37.0 38.0 29.0 38.0 14 35.71533336743361 38.0 37.0 38.0 29.0 38.0 15 35.716492575556195 38.0 37.0 38.0 29.0 38.0 16 35.6887357558388 38.0 37.0 38.0 29.0 38.0 17 35.63734546084065 38.0 37.0 38.0 29.0 38.0 18 35.64012914746406 38.0 37.0 38.0 29.0 38.0 19 35.66276488776797 38.0 37.0 38.0 29.0 38.0 20 35.59300296836291 38.0 37.0 38.0 29.0 38.0 21 35.596689420981626 38.0 37.0 38.0 29.0 38.0 22 35.62989526931124 38.0 37.0 38.0 29.0 38.0 23 35.62935780180534 38.0 37.0 38.0 29.0 38.0 24 35.637417312940215 38.0 37.0 38.0 29.0 38.0 25 35.65323305551959 38.0 37.0 38.0 29.0 38.0 26 35.65350252245723 38.0 37.0 38.0 29.0 38.0 27 35.646156098125644 38.0 37.0 38.0 29.0 38.0 28 35.65959528722683 38.0 37.0 38.0 29.0 38.0 29 35.625838515295825 38.0 37.0 38.0 29.0 38.0 30 35.62691112136803 38.0 37.0 38.0 29.0 38.0 31 35.66361400189541 38.0 37.0 38.0 29.0 38.0 32 35.671345650088554 38.0 37.0 38.0 29.0 38.0 33 35.635748584586956 38.0 37.0 38.0 29.0 38.0 34 35.60017351462604 38.0 37.0 38.0 29.0 38.0 35 35.595299389067385 38.0 37.0 38.0 29.0 38.0 36 35.57329263497003 38.0 37.0 38.0 29.0 38.0 37 35.55693554331875 38.0 37.0 38.0 29.0 38.0 38 35.54027810471585 38.0 37.0 38.0 29.0 38.0 39 35.542399768693166 38.0 37.0 38.0 29.0 38.0 40 35.513067291949575 38.0 37.0 38.0 29.0 38.0 41 35.50453491981849 38.0 37.0 38.0 28.0 38.0 42 35.39758639050503 38.0 37.0 38.0 28.0 38.0 43 35.439363512882785 38.0 37.0 38.0 28.0 38.0 44 35.417311732619794 38.0 37.0 38.0 28.0 38.0 45 35.41479794421914 38.0 37.0 38.0 28.0 38.0 46 35.365356626639674 38.0 37.0 38.0 28.0 38.0 47 35.27954745080741 38.0 37.0 38.0 27.0 38.0 48 35.226382762603684 38.0 37.0 38.0 27.0 38.0 49 35.13896014916944 38.0 37.0 38.0 26.0 38.0 50 34.833262588198885 38.0 36.0 38.0 25.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 23.0 4 581.0 5 1720.0 6 2521.0 7 1231.0 8 337.0 9 201.0 10 179.0 11 185.0 12 147.0 13 148.0 14 144.0 15 262.0 16 709.0 17 1907.0 18 8117.0 19 24624.0 20 47252.0 21 66410.0 22 82063.0 23 97171.0 24 112209.0 25 128023.0 26 145745.0 27 165126.0 28 188155.0 29 217360.0 30 251919.0 31 289899.0 32 340784.0 33 413461.0 34 531617.0 35 764698.0 36 1529234.0 37 6179097.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.088769955182684 18.321674157793023 13.959918554765622 23.629637332258675 2 13.809525000692213 21.881120744391204 38.88017165837493 25.42918259654166 3 18.409379105564707 28.59618680131273 29.40671816268402 23.58771593043854 4 12.409125496279092 18.29721704111546 36.24013265167031 33.05352481093514 5 12.823487179566815 38.08516657077644 34.23079341983383 14.860552829822923 6 30.19056700531262 35.400415310789526 19.373211651317444 15.035806032580412 7 25.41775606006865 32.72838892235704 23.805912759388136 18.047942258186172 8 27.95349500912764 31.84867948135915 21.480765746202515 18.717059763310697 9 27.925934397434766 14.921884137091709 20.05422213207258 37.09795933340095 10 16.730539028638844 28.76028514797742 31.50500837296959 23.004167450414144 11 31.85804575972433 24.522437042830038 21.82541696662713 21.7941002308185 12 23.507160418209754 26.71099990876359 29.8624798850473 19.919359787979356 13 29.64754363557197 21.338139124868054 25.348823879376635 23.665493360183337 14 24.071261434673982 21.351877935305726 26.47722051262662 28.09964011739367 15 26.724614903633764 25.196002997596434 25.864368256206454 22.21501384256335 16 22.35003048799436 26.849357542343093 28.19208092473574 22.608531044926806 17 23.092843467638787 26.647254626695887 27.88330470471782 22.3765972009475 18 24.47544858783635 24.03580259415741 30.52617747643286 20.962571341573383 19 22.926020217696504 27.66595355618 29.8841256599365 19.523900566186995 20 24.30540090480615 23.863916892902733 30.644455999372994 21.186226202918128 21 23.567179709385606 26.072864639325775 27.978219241281415 22.381736410007203 22 23.147324324065405 25.923585750890616 26.879780119570256 24.049309805473726 23 21.048128569323886 26.928495409662517 27.753212105603435 24.27016391541016 24 21.762987554961573 29.065093525517653 27.158211130811434 22.01370778870934 25 23.473144302729324 24.41246229892689 26.60055308430211 25.513840314041676 26 23.127041453288182 25.017928323817163 28.319044329069865 23.535985893824794 27 24.03276260780995 25.207265001342567 26.64174466229237 24.118227728555112 28 22.667891601072405 24.697344398017954 29.223661141372475 23.411102859537163 29 23.02407153442135 24.189366443346024 30.365349185369944 22.421212836862683 30 23.23705010618636 25.846085967191755 29.852737308775225 21.06412661784666 31 24.990231852283337 24.11390795215692 27.25746921296478 23.63839098259496 32 20.626371086496885 25.78869029381664 29.56630815525571 24.018630464430764 33 22.493709777945302 26.365177351420126 28.291045646327383 22.850067224307193 34 20.748875358599612 25.73040732220334 29.27532258968022 24.24539472951683 35 21.891128662289315 27.04129550378588 29.755729271997254 21.311846561927545 36 23.700045328990775 25.758630546630346 28.090432544903493 22.450891579475392 37 23.522601297500113 26.380229470604625 28.53152070452095 21.56564852737431 38 24.07612609614737 25.554488350619376 27.44612564469292 22.923259908540334 39 23.97533494606699 24.187198292180316 27.978581129289896 23.858885632462798 40 23.151349673638123 26.30128728752644 27.51478692398147 23.03257611485397 41 21.18769367610872 26.566064864026835 27.920176913505713 24.32606454635874 42 25.19604665605295 26.63374946647292 27.66231630808324 20.50788756939089 43 24.701609963296317 23.649813996006507 26.958703523166953 24.68987251753022 44 23.18090998607904 25.750012429428466 28.701440639811736 22.367636944680758 45 26.230982439382604 24.018150710458244 27.627415240550917 22.12345160960823 46 21.755466823117327 25.141904182941683 31.085571420598058 22.01705757334293 47 24.59860380932991 24.989725265150273 28.855794829677507 21.555876095842315 48 24.193799143832457 26.58572088951836 27.753111497282053 21.467368469367138 49 22.749851421046934 25.814766217645325 30.694373654866077 20.74100870644167 50 23.357045519616577 26.670977607291658 26.582124842275554 23.38985203081621 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 728.0 1 1343.0 2 1958.0 3 6604.0 4 11250.0 5 13576.5 6 15903.0 7 14236.0 8 12569.0 9 16407.5 10 20246.0 11 27379.0 12 34512.0 13 58991.5 14 83471.0 15 103149.5 16 122828.0 17 112110.0 18 101392.0 19 87014.0 20 72636.0 21 80547.5 22 88459.0 23 100283.0 24 112107.0 25 131717.0 26 151327.0 27 167947.5 28 184568.0 29 207252.0 30 229936.0 31 264355.5 32 298775.0 33 321134.5 34 343494.0 35 372660.5 36 401827.0 37 428508.5 38 455190.0 39 476823.0 40 498456.0 41 521326.0 42 544196.0 43 555299.0 44 566402.0 45 671512.0 46 776622.0 47 1018832.0 48 1261042.0 49 1295530.5 50 1330019.0 51 1093333.5 52 856648.0 53 688819.0 54 520990.0 55 534590.5 56 548191.0 57 516095.5 58 484000.0 59 461613.5 60 439227.0 61 366268.0 62 293309.0 63 254823.0 64 216337.0 65 183596.0 66 150855.0 67 134315.5 68 117776.0 69 99674.5 70 81573.0 71 66463.5 72 51354.0 73 47287.0 74 43220.0 75 33747.5 76 24275.0 77 20042.0 78 15809.0 79 12653.5 80 9498.0 81 8495.0 82 7492.0 83 6828.0 84 6164.0 85 4775.5 86 3387.0 87 3201.5 88 3016.0 89 1578.0 90 140.0 91 107.0 92 74.0 93 41.0 94 8.0 95 5.5 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044025584177839895 2 0.0 3 0.0 4 0.017242778756172014 5 0.01621632019089714 6 0.07073075827944497 7 0.07286993243228672 8 0.07945996893539599 9 0.0654173257062574 10 0.07726904056917905 11 0.0724472730230559 12 0.06876409817118724 13 0.076794626946573 14 0.07459507287812685 15 0.06880722668233324 16 0.07220575336063828 17 0.07500910658512848 18 0.08691257566142531 19 0.06783252233043358 20 0.06913500336704285 21 0.06731498019668154 22 0.056912383308265604 23 0.05913781448339936 24 0.06414934747856492 25 0.06939377443391889 26 0.05903430605664896 27 0.0625018383527876 28 0.053858884719128586 29 0.07006657920779653 30 0.06059555816013426 31 0.06151850829865873 32 0.05731779131303803 33 0.0640027105406685 34 0.06526206306613179 35 0.06652141559159508 36 0.05912918878117016 37 0.06285549214418482 38 0.07314595490362115 39 0.06866921544666603 40 0.06337303427793686 41 0.07625983340836257 42 0.06576235379542543 43 0.055299376991405094 44 0.06772038820145397 45 0.016104186061917533 46 0.00954002666549587 47 0.014827582131995842 48 0.021883406555481943 49 0.11693864512127264 50 0.009039735936202237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1.1593259E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.02451906488596 #Duplication Level Percentage of deduplicated Percentage of total 1 69.6748205605197 22.313026193828716 2 15.330798010605292 9.819228663410899 3 6.0614010791267505 5.8234036325524485 4 2.8914990648965686 3.7039546771952034 5 1.6212569274848143 2.5959986691657937 6 0.9581263560425857 1.84101214533933 7 0.6608293553329403 1.4813919601947234 8 0.4639094556945861 1.1885181766617714 9 0.33649091277667975 0.9698363686239893 >10 1.6901157464933838 9.757106527508403 >50 0.14424705478785213 3.2252003023786773 >100 0.13254608153441394 8.98034304242333 >500 0.019012851146367624 4.187365041055639 >1k 0.012536684372066703 7.473335404467147 >5k 0.0011913910582097573 2.5097260598381412 >10k+ 0.0012184681277145246 14.130553135355678 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 180513 1.557051386499689 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 146653 1.2649851090189566 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 104032 0.8973490543081976 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 93939 0.8102898417088759 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 82449 0.7111805230953608 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71309 0.6150902002620662 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 66934 0.5773527530093134 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 64731 0.5583503309983845 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 45673 0.39396169791427926 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 44654 0.3851721073427239 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 43307 0.3735532864399907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42428 0.3659712941805233 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 41772 0.3603128335181678 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 38718 0.3339699389101891 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35148 0.30317618195194296 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 33347 0.2876412922371526 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 29516 0.2545962269970851 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 27191 0.23454146931419373 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 26696 0.2302717467107394 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 26644 0.22982321019482096 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 23794 0.20523995884159923 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 22422 0.193405495383136 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 21670 0.18691896730677715 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 20357 0.17559342027983676 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 20356 0.17558479457760756 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 19573 0.16883086973214348 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 16624 0.14339367385823087 No Hit GTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCACTTTTTC 15881 0.13698477710193485 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 15874 0.13692439718633043 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 15813 0.1363982293503492 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 15487 0.13358625042362982 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 15067 0.12996345548736554 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 14866 0.12822968933929624 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 13932 0.12017328345722286 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 13644 0.11768908121521308 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 13521 0.11662811984102142 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12915 0.11140094429012584 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 12728 0.10978793797326533 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 12418 0.10711397028221314 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 12338 0.1064239141038771 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 12120 0.10454351101791136 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.312851114600304E-5 1.7251404458401214E-5 0.0 0.0 0.0 2 6.037991560440425E-5 2.587710668760182E-5 0.0 0.0 0.0 3 7.763132006280546E-5 2.587710668760182E-5 0.0 8.625702229200607E-6 0.0 4 7.763132006280546E-5 2.587710668760182E-5 0.0 3.450280891680243E-5 0.0 5 1.207598312088085E-4 5.175421337520364E-5 0.0 3.450280891680243E-5 0.0 6 1.380112356672097E-4 5.175421337520364E-5 0.0 3.450280891680243E-5 0.0 7 1.380112356672097E-4 5.175421337520364E-5 0.0 4.312851114600304E-5 0.0 8 1.5526264012561092E-4 5.175421337520364E-5 0.0 5.175421337520364E-5 0.0 9 1.6388834235481155E-4 5.175421337520364E-5 0.0 1.207598312088085E-4 0.0 10 2.501453646468176E-4 6.037991560440425E-5 0.0 2.1564255573001517E-4 0.0 11 3.1915098248042246E-4 6.037991560440425E-5 0.0 2.587710668760182E-4 0.0 12 3.277766847096231E-4 6.037991560440425E-5 0.0 3.0189957802202126E-4 0.0 13 3.277766847096231E-4 6.900561783360485E-5 0.0 3.277766847096231E-4 8.625702229200607E-6 14 3.277766847096231E-4 6.900561783360485E-5 0.0 3.3640238693882367E-4 8.625702229200607E-6 15 3.450280891680243E-4 6.900561783360485E-5 0.0 4.1403370700162913E-4 8.625702229200607E-6 16 4.1403370700162913E-4 6.900561783360485E-5 0.0 4.5716221814763217E-4 8.625702229200607E-6 17 4.4853651591843154E-4 6.900561783360485E-5 0.0 5.26167835981237E-4 8.625702229200607E-6 18 4.657879203768328E-4 6.900561783360485E-5 0.0 5.520449426688388E-4 8.625702229200607E-6 19 4.916650270644346E-4 6.900561783360485E-5 0.0 6.296762627316443E-4 8.625702229200607E-6 20 5.26167835981237E-4 6.900561783360485E-5 0.0 6.641790716484467E-4 8.625702229200607E-6 21 5.865477515856413E-4 6.900561783360485E-5 0.0 8.453188184616595E-4 8.625702229200607E-6 22 5.951734538148419E-4 6.900561783360485E-5 0.0 0.001164469800942082 8.625702229200607E-6 23 6.555533694192462E-4 8.625702229200608E-5 0.0 0.0015957549124021122 8.625702229200607E-6 24 6.81430476106848E-4 8.625702229200608E-5 0.0 0.0020270400238621426 8.625702229200607E-6 25 6.900561783360486E-4 8.625702229200608E-5 0.0 0.002173676961758553 8.625702229200607E-6 26 8.625702229200607E-4 8.625702229200608E-5 0.0 0.0025273307531557777 8.625702229200607E-6 27 9.315758407536656E-4 8.625702229200608E-5 0.0 0.0029068616512406046 8.625702229200607E-6 28 9.747043518996685E-4 1.0350842675040728E-4 0.0 0.004744136226060334 8.625702229200607E-6 29 9.919557563580698E-4 1.293855334380091E-4 0.0 0.006728047738776473 8.625702229200607E-6 30 0.001009207160816471 1.293855334380091E-4 0.0 0.010687245061979551 8.625702229200607E-6 31 0.0010695870764208754 1.293855334380091E-4 0.0 0.01592304631510432 8.625702229200607E-6 32 0.0010868384808792764 1.293855334380091E-4 0.0 0.02160738408414752 8.625702229200607E-6 33 0.001121341289796079 1.293855334380091E-4 0.0 0.02799040373375597 8.625702229200607E-6 34 0.0011472183964836807 1.293855334380091E-4 0.0 0.03571040722889051 8.625702229200607E-6 35 0.0011730955031712825 1.293855334380091E-4 0.0 0.04632002097080726 8.625702229200607E-6 36 0.0011730955031712825 1.293855334380091E-4 0.0 0.05791296476685288 8.625702229200607E-6 37 0.0012162240143172855 1.293855334380091E-4 0.0 0.0752247491408585 8.625702229200607E-6 38 0.0012593525254632886 1.6388834235481155E-4 0.0 0.09639222241131679 8.625702229200607E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGCG 1125 0.0 16.815182 19 AATGCGA 1190 0.0 16.45117 20 CGACGAA 1045 0.0 15.998635 38 TCGACGA 1040 0.0 15.863963 37 GGCGTTA 2405 0.0 15.826063 42 GTCGACG 1050 0.0 15.712877 36 GTACTAG 1540 0.0 15.5779 1 CATTGCG 1090 0.0 15.338206 29 CGACGAG 1210 0.0 15.270831 24 TGCGACG 1195 0.0 15.093708 22 AGTCGAC 1065 0.0 14.872548 35 CGGCGTT 2660 0.0 14.309396 41 CGAACAA 1240 0.0 14.194666 41 CGTCGTA 2485 0.0 13.89881 10 CTAACGC 825 0.0 13.86674 3 CAATTCG 1285 0.0 13.696393 15 GTATTAG 1885 0.0 13.660847 1 TCTAACG 855 0.0 13.637499 2 CGCGAAT 565 0.0 13.627686 35 CCGTCGT 2585 0.0 13.6164465 9 >>END_MODULE