FastQCFastQC Report
Wed 25 May 2016
SRR2558150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10468827
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA655420.6260682309488923No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT517990.4947927785987866No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC430660.41137369067231694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383300.36613462043073214No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA372060.3553979829831938No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG281200.26860697955941004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269380.2573163163361091No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG250980.23974032620846633No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG241450.23063710958257308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222920.2129369412638111No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA186920.17854913449233614No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT178210.17022919568734873No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC165250.15784958524961776No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC154840.14790577779153288No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC144580.13810525286166253No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC142120.1357554193989451No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT122360.11688033434882436No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG117560.11229529344596104No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC112670.10762428302616901No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG105350.10063209564930245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG5200.016.9228572
CGTCGTA29150.016.52761310
CTAACGC4950.016.4443113
TAGGTCG6300.016.4107621
CCGTCGT29750.016.1940529
TACCGTC31850.016.0945227
GGCGTTA28400.015.49394242
ATACCGT34650.015.174776
ACCGTCG33000.014.7998798
CTAGTAC12700.014.5510633
CGGCGTT31650.014.18174241
GTCGTAG34650.013.84062811
GTAAACG16350.013.58841527
CCTAGTA12500.013.3758282
CGTTATT34400.013.25080644
GCGGCGT34600.013.09953740
TGCGACG6250.013.02190222
CATTGCG8150.012.95546329
TAACGCC24000.012.7418674
ATACCGC34050.012.66205327
Produced by FastQC (version 0.11.5)