##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10468827
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.832308051322272	33.0	32.0	34.0	27.0	34.0
2	31.879621661529033	33.0	32.0	34.0	27.0	34.0
3	31.88749073797857	33.0	32.0	34.0	27.0	34.0
4	31.786219506731747	33.0	32.0	34.0	27.0	34.0
5	31.835737470874243	33.0	32.0	34.0	28.0	34.0
6	35.30968378787805	38.0	36.0	38.0	29.0	38.0
7	35.362569846650445	38.0	36.0	38.0	29.0	38.0
8	35.41149041817197	38.0	37.0	38.0	29.0	38.0
9	35.583587158332065	38.0	37.0	38.0	29.0	38.0
10	35.526523076558625	38.0	37.0	38.0	29.0	38.0
11	35.58444608932787	38.0	37.0	38.0	29.0	38.0
12	35.48409224834836	38.0	37.0	38.0	29.0	38.0
13	35.53796504613172	38.0	37.0	38.0	29.0	38.0
14	35.55130073311938	38.0	37.0	38.0	29.0	38.0
15	35.53368529253564	38.0	37.0	38.0	29.0	38.0
16	35.50232342171668	38.0	37.0	38.0	29.0	38.0
17	35.462448276201336	38.0	37.0	38.0	28.0	38.0
18	35.46155724991921	38.0	37.0	38.0	28.0	38.0
19	35.47370808592023	38.0	37.0	38.0	28.0	38.0
20	35.403427623744285	38.0	37.0	38.0	28.0	38.0
21	35.42748027071228	38.0	37.0	38.0	28.0	38.0
22	35.465124889350065	38.0	37.0	38.0	28.0	38.0
23	35.474010698619814	38.0	37.0	38.0	28.0	38.0
24	35.472073041229926	38.0	37.0	38.0	28.0	38.0
25	35.48747495779613	38.0	37.0	38.0	28.0	38.0
26	35.48777623319212	38.0	37.0	38.0	28.0	38.0
27	35.49192473999236	38.0	37.0	38.0	28.0	38.0
28	35.49863876822112	38.0	37.0	38.0	28.0	38.0
29	35.46424790475571	38.0	37.0	38.0	28.0	38.0
30	35.478064925516485	38.0	37.0	38.0	28.0	38.0
31	35.51538964202962	38.0	37.0	38.0	29.0	38.0
32	35.52372849412833	38.0	37.0	38.0	29.0	38.0
33	35.50233746340445	38.0	37.0	38.0	28.0	38.0
34	35.48352293910292	38.0	37.0	38.0	28.0	38.0
35	35.49218455897685	38.0	37.0	38.0	28.0	38.0
36	35.47237355245244	38.0	37.0	38.0	28.0	38.0
37	35.4666893435148	38.0	37.0	38.0	28.0	38.0
38	35.46798136983255	38.0	37.0	38.0	28.0	38.0
39	35.47822616612157	38.0	37.0	38.0	28.0	38.0
40	35.47694885014338	38.0	37.0	38.0	28.0	38.0
41	35.47800426924621	38.0	37.0	38.0	28.0	38.0
42	35.38915276754502	38.0	37.0	38.0	28.0	38.0
43	35.44025161558215	38.0	37.0	38.0	28.0	38.0
44	35.42581771577656	38.0	37.0	38.0	28.0	38.0
45	35.454873788629804	38.0	37.0	38.0	28.0	38.0
46	35.42549752708685	38.0	37.0	38.0	28.0	38.0
47	35.38167848222155	38.0	37.0	38.0	28.0	38.0
48	35.35180856460805	38.0	37.0	38.0	28.0	38.0
49	35.26978619476662	38.0	37.0	38.0	27.0	38.0
50	34.971029610098626	38.0	36.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	18.0
4	536.0
5	1400.0
6	2137.0
7	1052.0
8	260.0
9	214.0
10	146.0
11	129.0
12	115.0
13	135.0
14	118.0
15	204.0
16	535.0
17	1631.0
18	8218.0
19	26101.0
20	48602.0
21	67868.0
22	82835.0
23	95698.0
24	109364.0
25	123791.0
26	139569.0
27	156436.0
28	176031.0
29	200090.0
30	228804.0
31	264065.0
32	309307.0
33	375976.0
34	486494.0
35	700199.0
36	1402280.0
37	5458469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96721194448848	18.808028166873992	13.449227447431038	24.775532441206487
2	15.461407471916386	21.073354254492887	37.587916965291335	25.877321308299393
3	17.83750939813983	26.325891143296186	29.820370515244925	26.016228943319053
4	12.872460242676379	17.583313421910425	36.28288621082772	33.26134012458548
5	14.3755075368627	36.751123985165115	33.900796964236925	14.97257151373526
6	31.743189385029723	35.75298530745455	18.22032586037876	14.283499447136967
7	27.312024825090138	31.55025667976272	23.011823920340092	18.125894574807052
8	27.633029069965854	32.03309472332624	20.910059962701474	19.42381624400643
9	27.036344325610166	14.637535489070553	20.152019006007812	38.17410117931147
10	17.15508385149543	27.30784587923362	31.229786564087984	24.307283705182968
11	33.79520886342201	22.925840014613314	22.75419475604952	20.524756365915152
12	23.62425702940225	25.444625798217864	29.385285263240803	21.545831909139082
13	29.21196615870568	20.54206063451758	25.5562261752887	24.68974703148805
14	23.77274401378236	20.70548465901938	26.02522451631885	29.49654681087941
15	25.540690657939784	26.686772288728	23.879682839419534	23.892854213912678
16	24.134648453951375	26.29531982463274	25.919205115955318	23.650826605460566
17	23.321874099071714	26.666655195961702	26.742658175286138	23.26881252968045
18	24.446499218513463	24.710691284568075	28.42993432339155	22.412875173526913
19	24.1611500350167	26.36507540371	27.614273584848853	21.859500976424446
20	24.71083055375858	24.310525909307103	27.85681596079997	23.12182757613435
21	25.11422560668464	25.28215411878393	26.564390438330786	23.039229836200644
22	24.512082859134043	24.990786803185088	26.314763325971697	24.18236701170917
23	22.842132999918093	25.458392593578782	27.041834635075563	24.65763977142757
24	23.297513307061703	26.84400176248675	26.432235538576382	23.426249391875164
25	24.083971760385953	24.531943623619902	26.27067701225793	25.11340760373621
26	23.780448759302956	25.339417589674923	27.075953807056628	23.80417984396549
27	24.433094254872127	25.038608676902292	26.148124274922452	24.380172793303124
28	23.589437277263347	24.802013458096123	27.596866849714104	24.011682414926426
29	23.800078647327148	24.594992073137043	28.101775835235955	23.50315344429986
30	23.882229658912813	25.283751884526932	27.877904637346006	22.956113819214252
31	24.989758930088712	24.528409005107058	26.35141502626359	24.13041703854064
32	23.005317763639592	25.25129661166869	27.09152132997516	24.651864294716557
33	23.370566254863196	25.374004242765718	27.109065076609205	24.146364425761874
34	23.201148033880152	25.13655315902867	27.334431295889882	24.327867511201294
35	23.863795810707636	25.6352623306633	27.68486102280456	22.8160808358245
36	24.051986078222832	25.460468820127858	26.588851109315158	23.898693992334152
37	24.345302337104062	25.607855033867	26.947940357988177	23.09890227104076
38	24.217878809139776	25.159655097196282	26.657683986428864	23.964782107235077
39	24.449275856858034	24.504370016351416	26.648062351601098	24.39829177518945
40	24.387378415935515	25.612741057041465	26.480401605066877	23.519478921956143
41	23.036174964641	25.87050632867832	26.739119961366058	24.35419874531462
42	25.31458473438699	26.084630586356965	26.530103521536812	22.07068115771923
43	24.97534026421398	24.11866950105095	26.255639531766544	24.650350702968524
44	23.83604380920429	25.39401664183916	26.79948786988569	23.970451679070866
45	25.075165949774597	24.817407718397316	26.417715652545343	23.689710679282744
46	23.399907068204374	25.038355382344413	27.972709309572547	23.58902823987867
47	24.559577938470984	25.11232851218687	27.101258023925773	23.22683552541637
48	24.912831997492972	25.976743097308862	26.17297739374026	22.937447511457908
49	23.802531397172377	25.690478633608215	27.532829239374944	22.97416072984446
50	24.198497631753842	26.119336755422996	25.712463267370556	23.969702345452607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	937.0
1	1212.5
2	1488.0
3	4291.5
4	7095.0
5	7920.5
6	8746.0
7	7808.5
8	6871.0
9	8937.5
10	11004.0
11	15153.5
12	19303.0
13	34995.5
14	50688.0
15	59361.0
16	68034.0
17	64374.0
18	60714.0
19	53253.5
20	45793.0
21	51836.5
22	57880.0
23	68837.5
24	79795.0
25	95283.5
26	110772.0
27	127864.0
28	144956.0
29	169606.0
30	194256.0
31	219705.0
32	245154.0
33	269339.5
34	293525.0
35	325165.0
36	356805.0
37	392239.5
38	427674.0
39	460063.5
40	492453.0
41	516802.5
42	541152.0
43	552925.0
44	564698.0
45	626654.5
46	688611.0
47	797074.5
48	905538.0
49	916068.0
50	926598.0
51	802497.0
52	678396.0
53	611430.5
54	544465.0
55	563225.0
56	581985.0
57	563297.0
58	544609.0
59	508946.5
60	473284.0
61	418683.0
62	364082.0
63	319326.0
64	274570.0
65	235859.0
66	197148.0
67	177288.0
68	157428.0
69	135103.5
70	112779.0
71	89873.5
72	66968.0
73	63817.5
74	60667.0
75	46654.0
76	32641.0
77	27482.0
78	22323.0
79	18495.5
80	14668.0
81	12875.5
82	11083.0
83	10480.0
84	9877.0
85	7796.0
86	5715.0
87	5491.5
88	5268.0
89	2744.5
90	221.0
91	155.5
92	90.0
93	51.5
94	13.0
95	8.0
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044131018690059545
2	0.0
3	0.0
4	0.015627347743925848
5	0.015350334856044522
6	0.0661296628552559
7	0.06832666162121125
8	0.07473616671667227
9	0.061621039300773624
10	0.07311229806357485
11	0.06814517041880623
12	0.06467773323601583
13	0.07188006832093032
14	0.07009381280252315
15	0.0644293768537774
16	0.06805920090187755
17	0.07063828640973817
18	0.08247342324025414
19	0.06377027722399081
20	0.06490698528115901
21	0.06339774265063317
22	0.05325333965304804
23	0.05538347323916997
24	0.060532092086343574
25	0.06482101576423031
26	0.05499183432871706
27	0.058774493073579306
28	0.05002470668394845
29	0.06622518454073222
30	0.057207937431767664
31	0.05781927621881611
32	0.053434830855453044
33	0.06029328787265278
34	0.06130581773870177
35	0.062423421458774705
36	0.055354816733527074
37	0.05860255403972193
38	0.06889979173406915
39	0.06436251167394398
40	0.059318966680794324
41	0.07220484205154981
42	0.06173566532334521
43	0.051715440516879305
44	0.06344550349337132
45	0.015799286777783222
46	0.008931277592035861
47	0.014691235226257917
48	0.02155924441200528
49	0.11380453607648688
50	0.009112768794440868
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0468827E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.51980100674814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.54806874901462	25.39881631117653
2	15.394695260254432	11.244224149280411
3	6.03249144436402	6.60916161369254
4	2.815401856214484	4.112716621719291
5	1.5764397790134985	2.8785633514347495
6	0.9575612983169631	2.098196883977933
7	0.6711600869845574	1.7157442974243466
8	0.48325902889181943	1.4118818855874877
9	0.35545398458365834	1.1682997905645829
>10	1.8862124132948541	12.519025099825399
>50	0.14898277469881754	3.788766169826912
>100	0.10471602162502941	7.487084759176509
>500	0.012320885140545183	3.1413358786330066
>1k	0.011529340107417274	8.725875058581796
>5k	0.0011292974199889766	2.6552088817076074
>10k+	5.777800753431972E-4	5.045099247390936
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	65542	0.6260682309488923	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	51799	0.4947927785987866	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	43066	0.41137369067231694	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38330	0.36613462043073214	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	37206	0.3553979829831938	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	28120	0.26860697955941004	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26938	0.2573163163361091	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	25098	0.23974032620846633	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	24145	0.23063710958257308	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22292	0.2129369412638111	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	18692	0.17854913449233614	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	17821	0.17022919568734873	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	16525	0.15784958524961776	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	15484	0.14790577779153288	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	14458	0.13810525286166253	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	14212	0.1357554193989451	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	12236	0.11688033434882436	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	11756	0.11229529344596104	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	11267	0.10762428302616901	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	10535	0.10063209564930245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.552168547631936E-6	0.0	0.0	0.0	1.9104337095263872E-5
2	9.552168547631936E-6	9.552168547631936E-6	0.0	9.552168547631936E-6	1.9104337095263872E-5
3	9.552168547631936E-6	9.552168547631936E-6	0.0	9.552168547631936E-6	1.9104337095263872E-5
4	9.552168547631936E-6	9.552168547631936E-6	0.0	9.552168547631936E-6	1.9104337095263872E-5
5	9.552168547631936E-6	9.552168547631936E-6	0.0	9.552168547631936E-6	1.9104337095263872E-5
6	1.9104337095263872E-5	9.552168547631936E-6	0.0	9.552168547631936E-6	1.9104337095263872E-5
7	2.8656505642895808E-5	9.552168547631936E-6	0.0	1.9104337095263872E-5	1.9104337095263872E-5
8	3.8208674190527743E-5	9.552168547631936E-6	0.0	3.8208674190527743E-5	2.8656505642895808E-5
9	3.8208674190527743E-5	9.552168547631936E-6	0.0	3.8208674190527743E-5	2.8656505642895808E-5
10	6.686517983342355E-5	9.552168547631936E-6	0.0	6.686517983342355E-5	2.8656505642895808E-5
11	6.686517983342355E-5	1.9104337095263872E-5	0.0	9.552168547631937E-5	2.8656505642895808E-5
12	6.686517983342355E-5	2.8656505642895808E-5	0.0	1.050738540239513E-4	2.8656505642895808E-5
13	6.686517983342355E-5	2.8656505642895808E-5	0.0	1.2417819111921517E-4	3.8208674190527743E-5
14	6.686517983342355E-5	2.8656505642895808E-5	0.0	1.4328252821447905E-4	3.8208674190527743E-5
15	6.686517983342355E-5	2.8656505642895808E-5	0.0	1.6238686530974293E-4	3.8208674190527743E-5
16	9.552168547631937E-5	2.8656505642895808E-5	0.0	1.6238686530974293E-4	5.7313011285791615E-5
17	1.050738540239513E-4	2.8656505642895808E-5	0.0	1.7193903385737485E-4	5.7313011285791615E-5
18	1.050738540239513E-4	2.8656505642895808E-5	0.0	1.7193903385737485E-4	5.7313011285791615E-5
19	1.050738540239513E-4	2.8656505642895808E-5	0.0	1.9104337095263873E-4	5.7313011285791615E-5
20	1.1462602257158323E-4	2.8656505642895808E-5	0.0	2.1969987659553453E-4	5.7313011285791615E-5
21	1.2417819111921517E-4	2.8656505642895808E-5	0.0	2.9611722497659E-4	6.686517983342355E-5
22	1.337303596668471E-4	2.8656505642895808E-5	0.0	4.680562588339649E-4	6.686517983342355E-5
23	1.6238686530974293E-4	2.8656505642895808E-5	0.0	8.883516749297701E-4	6.686517983342355E-5
24	2.101477080479026E-4	2.8656505642895808E-5	0.0	0.0010698428773347768	6.686517983342355E-5
25	2.2925204514316646E-4	2.8656505642895808E-5	0.0	0.0011271558886205685	6.686517983342355E-5
26	2.7701288788132617E-4	2.8656505642895808E-5	0.0	0.0012608862482874155	6.686517983342355E-5
27	2.9611722497659E-4	2.8656505642895808E-5	0.0	0.0014805861248829502	7.641734838105549E-5
28	3.152215620718539E-4	3.8208674190527743E-5	0.0	0.002378489968360352	7.641734838105549E-5
29	3.343258991671178E-4	3.8208674190527743E-5	0.0	0.0033337068231235455	7.641734838105549E-5
30	3.343258991671178E-4	3.8208674190527743E-5	0.0	0.005244140532649933	7.641734838105549E-5
31	3.7253457335764554E-4	3.8208674190527743E-5	0.0	0.008014269411463194	7.641734838105549E-5
32	3.7253457335764554E-4	3.8208674190527743E-5	0.0	0.010593354919323818	7.641734838105549E-5
33	4.011910790005413E-4	3.8208674190527743E-5	0.0	0.01361184018037551	7.641734838105549E-5
34	4.1074324754817324E-4	3.8208674190527743E-5	0.0	0.017528229284904603	7.641734838105549E-5
35	4.1074324754817324E-4	3.8208674190527743E-5	0.0	0.023230873907840868	7.641734838105549E-5
36	4.202954160958052E-4	3.8208674190527743E-5	0.0	0.02904814455334872	8.596951692868743E-5
37	4.48951921738701E-4	3.8208674190527743E-5	0.0	0.038523895752599596	8.596951692868743E-5
38	4.585040902863329E-4	4.776084273815968E-5	0.0	0.04866829875018471	1.050738540239513E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACG	520	0.0	16.922857	2
CGTCGTA	2915	0.0	16.527613	10
CTAACGC	495	0.0	16.444311	3
TAGGTCG	630	0.0	16.41076	21
CCGTCGT	2975	0.0	16.194052	9
TACCGTC	3185	0.0	16.094522	7
GGCGTTA	2840	0.0	15.493942	42
ATACCGT	3465	0.0	15.17477	6
ACCGTCG	3300	0.0	14.799879	8
CTAGTAC	1270	0.0	14.551063	3
CGGCGTT	3165	0.0	14.181742	41
GTCGTAG	3465	0.0	13.840628	11
GTAAACG	1635	0.0	13.588415	27
CCTAGTA	1250	0.0	13.375828	2
CGTTATT	3440	0.0	13.250806	44
GCGGCGT	3460	0.0	13.099537	40
TGCGACG	625	0.0	13.021902	22
CATTGCG	815	0.0	12.955463	29
TAACGCC	2400	0.0	12.741867	4
ATACCGC	3405	0.0	12.662053	27
>>END_MODULE