##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10678772
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92017836882368	33.0	32.0	34.0	27.0	34.0
2	31.959906157749224	33.0	32.0	34.0	27.0	34.0
3	31.966184220432837	33.0	32.0	34.0	28.0	34.0
4	31.869204530258724	33.0	32.0	34.0	28.0	34.0
5	31.921983258000076	33.0	33.0	34.0	28.0	34.0
6	35.429209182479035	38.0	36.0	38.0	29.0	38.0
7	35.49511385766079	38.0	37.0	38.0	29.0	38.0
8	35.55829911903728	38.0	37.0	38.0	29.0	38.0
9	35.71320897196794	38.0	37.0	38.0	29.0	38.0
10	35.65641021270985	38.0	37.0	38.0	29.0	38.0
11	35.714955895677896	38.0	37.0	38.0	29.0	38.0
12	35.62154159673041	38.0	37.0	38.0	29.0	38.0
13	35.676075676117065	38.0	37.0	38.0	29.0	38.0
14	35.68635560343455	38.0	37.0	38.0	29.0	38.0
15	35.68330094508994	38.0	37.0	38.0	29.0	38.0
16	35.6494126852788	38.0	37.0	38.0	29.0	38.0
17	35.60904381140453	38.0	37.0	38.0	29.0	38.0
18	35.614053563462164	38.0	37.0	38.0	29.0	38.0
19	35.626060000157324	38.0	37.0	38.0	29.0	38.0
20	35.56252732055708	38.0	37.0	38.0	29.0	38.0
21	35.57478219405752	38.0	37.0	38.0	29.0	38.0
22	35.61673711172033	38.0	37.0	38.0	29.0	38.0
23	35.61876178272183	38.0	37.0	38.0	29.0	38.0
24	35.620147241649136	38.0	37.0	38.0	29.0	38.0
25	35.633399795407186	38.0	37.0	38.0	29.0	38.0
26	35.639115902090616	38.0	37.0	38.0	29.0	38.0
27	35.64209330436121	38.0	37.0	38.0	29.0	38.0
28	35.65010658528902	38.0	37.0	38.0	29.0	38.0
29	35.615080554206045	38.0	37.0	38.0	29.0	38.0
30	35.623441815219955	38.0	37.0	38.0	29.0	38.0
31	35.65986454247736	38.0	37.0	38.0	29.0	38.0
32	35.666159367387934	38.0	37.0	38.0	29.0	38.0
33	35.64372738738125	38.0	37.0	38.0	29.0	38.0
34	35.61949548131564	38.0	37.0	38.0	29.0	38.0
35	35.6254903653716	38.0	37.0	38.0	29.0	38.0
36	35.6038207389389	38.0	37.0	38.0	29.0	38.0
37	35.59649648854756	38.0	37.0	38.0	29.0	38.0
38	35.59335989194263	38.0	37.0	38.0	29.0	38.0
39	35.60155802558571	38.0	37.0	38.0	29.0	38.0
40	35.59563131416234	38.0	37.0	38.0	29.0	38.0
41	35.589337051114114	38.0	37.0	38.0	29.0	38.0
42	35.4987323448801	38.0	37.0	38.0	28.0	38.0
43	35.54402781518324	38.0	37.0	38.0	29.0	38.0
44	35.526962276186815	38.0	37.0	38.0	29.0	38.0
45	35.544269977858875	38.0	37.0	38.0	29.0	38.0
46	35.502933857937975	38.0	37.0	38.0	28.0	38.0
47	35.45004790813026	38.0	37.0	38.0	28.0	38.0
48	35.41324873309403	38.0	37.0	38.0	28.0	38.0
49	35.32510423483149	38.0	37.0	38.0	27.0	38.0
50	35.03367774871493	38.0	36.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	14.0
4	463.0
5	1432.0
6	2243.0
7	1131.0
8	262.0
9	192.0
10	184.0
11	138.0
12	129.0
13	123.0
14	146.0
15	210.0
16	540.0
17	1517.0
18	6354.0
19	20117.0
20	39406.0
21	58101.0
22	73872.0
23	88574.0
24	104004.0
25	119284.0
26	135474.0
27	153431.0
28	174673.0
29	200248.0
30	230397.0
31	265400.0
32	312785.0
33	379486.0
34	490286.0
35	707103.0
36	1413800.0
37	5697253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.374240066088824	18.73312448549618	13.606752932021838	24.285882516393155
2	15.210063479209031	21.531998248487746	38.10923203529394	25.14870623700927
3	17.957195827385398	26.96367147833103	30.216629777281508	24.862502917002065
4	12.761998849302561	17.748819589167482	36.37796111886454	33.11122044266542
5	13.83633202259214	37.32926296446669	34.01613630584523	14.818268707095939
6	30.963222564511028	35.88370093589308	18.678795403699063	14.47428109589683
7	26.464161469892694	32.21030601149019	23.26413005872539	18.061402459891728
8	27.363433703302885	32.10149990797306	21.186626517179423	19.34843987154463
9	27.140100562896226	14.823689785550714	20.149263591034853	37.886946060518206
10	16.85352625111799	27.797992603460465	31.34744693436452	24.001034211057025
11	33.154875459006135	23.283139479203623	22.857966685989297	20.70401837580095
12	23.39201227609098	26.04235217654452	29.481303109006625	21.084332438357876
13	28.887759552415403	20.800675402770473	26.0402760162482	24.271289028565924
14	23.75426557194391	21.205703766073057	26.22028741860682	28.819743243376212
15	25.700554769061117	26.609546180841747	24.613674433283595	23.07622461681354
16	23.679316757352435	26.760299546500875	26.328643724174704	23.231739971971983
17	23.091537616916952	26.92935517779117	26.974064540227786	23.00504266506409
18	23.90120765938112	24.74953832727142	29.265318049960378	22.083935963387084
19	23.719172393217043	26.658463213020177	28.612974150500264	21.00939024326251
20	24.723016634557	24.098161877703397	28.830624002039023	22.348197485700577
21	24.648372866534896	25.562169566259534	26.988590097524746	22.80086746968082
22	23.94680864719269	25.41161525803445	26.74019042232966	23.901385672443197
23	22.07940527651996	26.105155989173216	27.423199102915152	24.392239631391675
24	23.000140176652568	27.386751132469932	26.85846505815832	22.75464363271918
25	23.73372926890291	24.547118238117953	26.703986311549066	25.01516618143007
26	23.498680009984252	25.34669414084841	27.695954054453146	23.458671794714192
27	24.14168321007336	25.04056011991272	26.63406802706301	24.183688642950916
28	22.972046946199704	25.027667107266677	28.49446901452631	23.505816932007313
29	23.368741824124502	24.694048899700515	28.94176527693963	22.995443999235356
30	23.223120408927	25.864834987942036	28.591833265964645	22.32021133716632
31	24.724546557797062	24.738629370356794	26.837361973445233	23.69946209840091
32	22.25315278943361	25.475336898900768	27.86798924277118	24.40352106889444
33	23.02560835058984	25.70369086326284	27.600770218228497	23.66993056791882
34	22.407472599509585	25.549319289819692	27.93317530851056	24.110032802160163
35	23.36400941112444	26.04920085183251	28.302732061531565	22.284057675511484
36	23.59109579324934	25.83135181608422	27.148803903156416	23.428748487510024
37	24.182259453681514	25.775463492189616	27.49671678196659	22.545560272162284
38	24.098812823248153	25.318956295012974	27.188707202300588	23.39352367943829
39	24.230842320567355	24.53093216479613	27.201099270563816	24.0371262440727
40	23.88029409813541	25.861824699251034	27.04737360710231	23.210507595511245
41	22.2821697266883	26.341394524957025	27.36395069891743	24.012485049437245
42	25.191541101198055	26.16956590263867	27.0388213328146	21.60007166334868
43	24.560992904080283	24.1346062315472	26.822494072991958	24.48190679138056
44	23.38865482712486	25.5399823949238	27.446340031345805	23.62502274660553
45	25.219611650794597	24.802496323220545	26.930884038923146	23.047007987061708
46	22.6897525183365	25.481514030591462	28.872476770205928	22.95625668086611
47	24.16998905809419	25.240025590741627	27.774664734967146	22.815320616197035
48	24.588335194425603	26.170909900842275	26.931737908004905	22.309016996727213
49	23.29172960850761	25.94830420547592	28.624528938152977	22.135437247863493
50	23.907829671654703	26.30108600564182	26.296927827418372	23.494156495285104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1211.0
1	1435.0
2	1659.0
3	5004.5
4	8350.0
5	9506.0
6	10662.0
7	9601.0
8	8540.0
9	11045.5
10	13551.0
11	18705.0
12	23859.0
13	42650.0
14	61441.0
15	74306.0
16	87171.0
17	82451.0
18	77731.0
19	68653.5
20	59576.0
21	67671.5
22	75767.0
23	88338.0
24	100909.0
25	114592.5
26	128276.0
27	141411.0
28	154546.0
29	174650.0
30	194754.0
31	234997.5
32	275241.0
33	297449.5
34	319658.0
35	348376.0
36	377094.0
37	409169.0
38	441244.0
39	472317.5
40	503391.0
41	531812.5
42	560234.0
43	558284.0
44	556334.0
45	619783.0
46	683232.0
47	821992.0
48	960752.0
49	978310.0
50	995868.0
51	870296.5
52	744725.0
53	641376.5
54	538028.0
55	553247.0
56	568466.0
57	538502.0
58	508538.0
59	476532.0
60	444526.0
61	383402.0
62	322278.0
63	283122.0
64	243966.0
65	207328.0
66	170690.0
67	155882.5
68	141075.0
69	121143.5
70	101212.0
71	81046.5
72	60881.0
73	58836.0
74	56791.0
75	43391.0
76	29991.0
77	25382.5
78	20774.0
79	17025.5
80	13277.0
81	11865.0
82	10453.0
83	10236.5
84	10020.0
85	7935.0
86	5850.0
87	5838.0
88	5826.0
89	3028.0
90	230.0
91	167.5
92	105.0
93	57.5
94	10.0
95	8.5
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04357242574333453
2	0.0
3	0.0
4	0.01656557514290969
5	0.01504854678047251
6	0.06692717102678097
7	0.06937127227737422
8	0.07476515089937308
9	0.06222625597774726
10	0.07337922375344282
11	0.06879068117570072
12	0.06527904144783689
13	0.0730982925752137
14	0.07095385124806486
15	0.06502620338743069
16	0.0684254706440029
17	0.07144079862366197
18	0.0831181712653852
19	0.064089766126667
20	0.06566298072475
21	0.06380883494843789
22	0.053929421847380955
23	0.055998948193668706
24	0.06109316689222318
25	0.06556933699867362
26	0.05584911823194652
27	0.05939821545024091
28	0.05101710196640587
29	0.06678670543766642
30	0.05766580651782808
31	0.05794673769605719
32	0.0539668793378115
33	0.06060621951662607
34	0.06188913856387232
35	0.06293794829592766
36	0.055942761958022884
37	0.05914537738983471
38	0.06933381478694367
39	0.06525094833001398
40	0.060081814650598406
41	0.07275181078873114
42	0.06227307784078544
43	0.05240302911233614
44	0.06414595236231282
45	0.015254562977840523
46	0.008334291620796849
47	0.013793720851049164
48	0.021032380876752497
49	0.11404869398840989
50	0.00954229568718201
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0678772E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.040184393438246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21019252743747	23.19757707405334
2	15.570842055902723	10.28926985376258
3	5.816220049692775	5.765069487439854
4	2.7209090636114444	3.5959734871799807
5	1.475102840946146	2.436883493207264
6	0.910269528559561	1.8045283842801598
7	0.597633316322429	1.3822140479668543
8	0.4084944224825274	1.0797384834011017
9	0.3080991520071854	0.9161687514401428
>10	1.6552064444095422	10.05153492355017
>50	0.1532249548085033	3.5368432804731924
>100	0.13256159260307726	9.051955145684977
>500	0.02079390884108464	4.786812530365355
>1k	0.018092695485478275	11.982211835350439
>5k	0.0015905665764331094	3.4780163862678593
>10k+	7.66880313637392E-4	6.6452028355766375
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	85288	0.7986686109601366	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	67774	0.6346609891099838	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	51053	0.47807931473768706	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48844	0.45739341564741715	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	44119	0.4131467550763327	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	38049	0.3563050133479767	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32459	0.30395817047128637	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	31971	0.29938835663875957	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	31335	0.2934326156603025	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27687	0.2592713843876431	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	22934	0.21476252138354487	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	21641	0.20265438760187032	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	21377	0.2001821932334542	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	20525	0.19220374777174754	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18271	0.17109645191413395	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	16423	0.15379109133522093	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	14078	0.13183163757031238	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	13185	0.12346925283169263	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	12849	0.12032282363552663	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	12798	0.11984524063253715	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	11406	0.10681003396270658	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	11273	0.10556457240589086	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	10779	0.10093857233771823	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	10744	0.10061081929645094	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG	10690	0.10010514317563854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6186235645821446E-5	9.364372607636908E-6	0.0	0.0	0.0
2	6.555060825345835E-5	1.8728745215273815E-5	0.0	1.8728745215273815E-5	0.0
3	7.491498086109526E-5	2.8093117822910723E-5	0.0	5.6186235645821446E-5	0.0
4	7.491498086109526E-5	3.745749043054763E-5	0.0	1.03008098684006E-4	0.0
5	9.364372607636908E-5	3.745749043054763E-5	0.0	1.1237247129164289E-4	0.0
6	9.364372607636908E-5	3.745749043054763E-5	0.0	1.1237247129164289E-4	0.0
7	9.364372607636908E-5	3.745749043054763E-5	0.0	1.217368438992798E-4	0.0
8	9.364372607636908E-5	3.745749043054763E-5	0.0	1.311012165069167E-4	0.0
9	1.03008098684006E-4	3.745749043054763E-5	0.0	1.9665182476037507E-4	0.0
10	2.2474494258328578E-4	3.745749043054763E-5	0.0	3.371174138749287E-4	9.364372607636908E-6
11	2.9029555083674415E-4	6.555060825345835E-5	0.0	3.558461590902025E-4	9.364372607636908E-6
12	2.9965992344438105E-4	6.555060825345835E-5	0.0	3.558461590902025E-4	9.364372607636908E-6
13	3.0902429605201794E-4	6.555060825345835E-5	0.0	3.6521053169783944E-4	9.364372607636908E-6
14	3.0902429605201794E-4	7.491498086109526E-5	0.0	3.7457490430547634E-4	9.364372607636908E-6
15	3.0902429605201794E-4	7.491498086109526E-5	0.0	4.12032394736024E-4	9.364372607636908E-6
16	3.277530412672918E-4	7.491498086109526E-5	0.0	4.4948988516657157E-4	9.364372607636908E-6
17	3.558461590902025E-4	7.491498086109526E-5	9.364372607636908E-6	4.775830029894823E-4	9.364372607636908E-6
18	3.558461590902025E-4	1.03008098684006E-4	9.364372607636908E-6	5.244048660276668E-4	9.364372607636908E-6
19	3.558461590902025E-4	1.03008098684006E-4	9.364372607636908E-6	5.524979838505776E-4	9.364372607636908E-6
20	3.6521053169783944E-4	1.03008098684006E-4	9.364372607636908E-6	6.648704551422205E-4	9.364372607636908E-6
21	3.8393927691311323E-4	1.03008098684006E-4	9.364372607636908E-6	7.678785538262265E-4	1.8728745215273815E-5
22	4.307611399512978E-4	1.03008098684006E-4	9.364372607636908E-6	9.270728881560539E-4	1.8728745215273815E-5
23	4.869473755971192E-4	1.311012165069167E-4	9.364372607636908E-6	0.0015544858528677267	1.8728745215273815E-5
24	5.150404934200299E-4	1.4046558911455363E-4	9.364372607636908E-6	0.001966518247603751	1.8728745215273815E-5
25	5.431336112429407E-4	1.4046558911455363E-4	9.364372607636908E-6	0.0021257125819335782	1.8728745215273815E-5
26	6.742348277498574E-4	1.4046558911455363E-4	9.364372607636908E-6	0.0024721943684161436	1.8728745215273815E-5
27	7.304210633956789E-4	1.4046558911455363E-4	9.364372607636908E-6	0.0028374049001139833	1.8728745215273815E-5
28	7.397854360033158E-4	1.5919433432982745E-4	9.364372607636908E-6	0.003961129613030412	1.8728745215273815E-5
29	7.491498086109527E-4	1.7792307954510124E-4	9.364372607636908E-6	0.005515615465898139	1.8728745215273815E-5
30	7.491498086109527E-4	1.7792307954510124E-4	9.364372607636908E-6	0.00805336044256774	1.8728745215273815E-5
31	8.053360442567741E-4	1.7792307954510124E-4	9.364372607636908E-6	0.01133089085524066	1.8728745215273815E-5
32	8.14700416864411E-4	1.7792307954510124E-4	9.364372607636908E-6	0.015039182407864874	1.8728745215273815E-5
33	8.427935346873218E-4	1.7792307954510124E-4	9.364372607636908E-6	0.01926251445390912	1.8728745215273815E-5
34	8.802510251178693E-4	1.7792307954510124E-4	9.364372607636908E-6	0.02465639307590798	1.8728745215273815E-5
35	8.802510251178693E-4	1.7792307954510124E-4	9.364372607636908E-6	0.032634838537614626	1.8728745215273815E-5
36	8.896153977255062E-4	1.8728745215273817E-4	9.364372607636908E-6	0.04056646213628309	1.8728745215273815E-5
37	9.270728881560539E-4	1.9665182476037507E-4	9.364372607636908E-6	0.05274014652621107	1.8728745215273815E-5
38	9.364372607636908E-4	2.153805699756489E-4	9.364372607636908E-6	0.06577535319604164	1.8728745215273815E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTTA	3320	0.0	15.837746	42
CCGTCGT	3390	0.0	15.250119	9
CGTCGTA	3405	0.0	15.247618	10
ATACCGT	3695	0.0	15.182775	6
GTACTAG	1035	0.0	15.098603	1
TACCGTC	3550	0.0	15.05932	7
CGGCGTT	3600	0.0	14.851207	41
GTATTAG	2015	0.0	14.41842	1
AATGCGA	780	0.0	14.38228	20
ACCGTCG	3675	0.0	14.307238	8
CAATGCG	870	0.0	14.158619	19
ACGCTTC	2095	0.0	13.859453	31
CGACGAG	860	0.0	13.812228	24
TAGACCG	160	6.352315E-6	13.750215	5
GTCGTAG	3865	0.0	13.71749	11
GTAAACG	2040	0.0	13.69456	27
TGCGACG	820	0.0	13.680897	22
GCGTTAT	3900	0.0	13.606435	43
GTCCTAT	3075	0.0	13.599637	1
GCGGCGT	3940	0.0	13.45763	40
>>END_MODULE