##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10568606
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973674295361185	33.0	32.0	34.0	27.0	34.0
2	32.00097221904194	33.0	32.0	34.0	28.0	34.0
3	31.999787483798716	33.0	32.0	34.0	28.0	34.0
4	31.901017693345747	33.0	33.0	34.0	28.0	34.0
5	31.947417095499635	33.0	33.0	34.0	28.0	34.0
6	35.43493020744647	38.0	36.0	38.0	29.0	38.0
7	35.499059573230376	38.0	37.0	38.0	29.0	38.0
8	35.555668647312615	38.0	37.0	38.0	29.0	38.0
9	35.701807882704685	38.0	37.0	38.0	29.0	38.0
10	35.628454121574784	38.0	37.0	38.0	29.0	38.0
11	35.68701198625438	38.0	37.0	38.0	29.0	38.0
12	35.59411307413674	38.0	37.0	38.0	29.0	38.0
13	35.64915864968379	38.0	37.0	38.0	29.0	38.0
14	35.67508562624059	38.0	37.0	38.0	29.0	38.0
15	35.67629940978025	38.0	37.0	38.0	29.0	38.0
16	35.650647398531085	38.0	37.0	38.0	29.0	38.0
17	35.596726285377656	38.0	37.0	38.0	29.0	38.0
18	35.605602479645846	38.0	37.0	38.0	29.0	38.0
19	35.62562375775954	38.0	37.0	38.0	29.0	38.0
20	35.55123655853951	38.0	37.0	38.0	29.0	38.0
21	35.55977808236961	38.0	37.0	38.0	29.0	38.0
22	35.588178043537624	38.0	37.0	38.0	29.0	38.0
23	35.59227196093789	38.0	37.0	38.0	29.0	38.0
24	35.597250384771655	38.0	37.0	38.0	29.0	38.0
25	35.610506343031425	38.0	37.0	38.0	29.0	38.0
26	35.61465334217209	38.0	37.0	38.0	29.0	38.0
27	35.60744529600214	38.0	37.0	38.0	29.0	38.0
28	35.619609624959054	38.0	37.0	38.0	29.0	38.0
29	35.58407447491183	38.0	37.0	38.0	29.0	38.0
30	35.586015695920544	38.0	37.0	38.0	29.0	38.0
31	35.62412583078601	38.0	37.0	38.0	29.0	38.0
32	35.62617700006983	38.0	37.0	38.0	29.0	38.0
33	35.59157120626883	38.0	37.0	38.0	29.0	38.0
34	35.55616729396478	38.0	37.0	38.0	29.0	38.0
35	35.55185139837742	38.0	37.0	38.0	29.0	38.0
36	35.529192118619996	38.0	37.0	38.0	29.0	38.0
37	35.50918862903963	38.0	37.0	38.0	29.0	38.0
38	35.49271569022442	38.0	37.0	38.0	28.0	38.0
39	35.489728919783744	38.0	37.0	38.0	28.0	38.0
40	35.46541918584154	38.0	37.0	38.0	28.0	38.0
41	35.45613991097785	38.0	37.0	38.0	28.0	38.0
42	35.35153765785194	38.0	37.0	38.0	28.0	38.0
43	35.38811703265312	38.0	37.0	38.0	28.0	38.0
44	35.361526013932206	38.0	37.0	38.0	28.0	38.0
45	35.356382194586494	38.0	37.0	38.0	28.0	38.0
46	35.30221469132258	38.0	37.0	38.0	27.0	38.0
47	35.218082119817886	38.0	37.0	38.0	27.0	38.0
48	35.16110932700112	38.0	37.0	38.0	27.0	38.0
49	35.0678466015291	38.0	37.0	38.0	25.0	38.0
50	34.758393491062115	38.0	36.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	25.0
4	541.0
5	1489.0
6	2258.0
7	1114.0
8	269.0
9	207.0
10	181.0
11	137.0
12	117.0
13	142.0
14	111.0
15	220.0
16	581.0
17	1935.0
18	8456.0
19	25366.0
20	46406.0
21	63587.0
22	76847.0
23	89175.0
24	103019.0
25	117838.0
26	133122.0
27	151511.0
28	172773.0
29	201369.0
30	232507.0
31	266688.0
32	314546.0
33	380944.0
34	493171.0
35	710911.0
36	1415437.0
37	5555606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.540374979150684	18.1366927763569	13.747572628047951	23.575359616444466
2	14.422375098475618	21.80954612178749	37.85070613853899	25.9173726411979
3	18.41040341554979	28.1205865749939	29.630378878728187	23.83863113072812
4	12.52770221123692	17.994309749569553	35.916431449838484	33.56155658935505
5	13.34227692771256	37.828716603354586	33.7799578630273	15.049048605905554
6	30.76270475497198	35.157550001978926	18.947898560042237	15.131846683006856
7	26.046672551779825	32.46082727470623	23.365534175334393	18.126965998179546
8	28.764406117773078	30.791811404899267	21.321518299798758	19.1222641775289
9	28.138536294951976	14.413605454027984	19.90056317614607	37.547295074873965
10	17.207513145277126	28.660278731292436	30.72983192207061	23.40237620135982
11	32.49018568141576	24.072123168988078	21.690751910312372	21.746939239283787
12	24.045767014190133	26.619238397450058	29.201392265279164	20.13360232308065
13	30.169547345054266	20.78179096758163	25.035374246412317	24.013287440951782
14	24.33349124301941	21.185328242726538	26.36937098761288	28.111809526641174
15	27.020511008901295	25.38302564815847	25.312694722595726	22.283768620344517
16	22.76874850809292	26.272600883218463	28.01042348903959	22.94822711964903
17	23.531019251526175	26.46818062552949	27.386456636129747	22.61434348681459
18	24.702432248457615	23.45502846127301	30.559759203273757	21.28278008699562
19	23.639285192855866	26.87154562870954	29.69099052815582	19.798178650278775
20	24.81595859368979	23.511340548608615	30.12831911279118	21.54438174491042
21	24.323447617395637	25.362538530962436	27.96533605210405	22.348677799537874
22	23.810211763636637	25.345867352348638	26.850981565354378	23.992939318660344
23	21.65574896187051	26.2031447647798	27.680218342103817	24.460887931245868
24	22.03817075257142	28.41151373310316	27.207163518214088	22.34315199611133
25	23.655558352946368	24.195343262734617	26.505977552848197	25.643120831470817
26	23.725219632200385	24.6093815458301	28.208873903022425	23.456524918947093
27	24.28298188644618	24.644594005801473	26.729179846595567	24.343244261156777
28	23.22464103918362	24.24278341287625	29.105105044835017	23.427470503105113
29	23.50896325891859	23.870913619065213	30.054378243476027	22.56574487854017
30	23.789969148864255	25.289192710626267	29.413707235540933	21.50713090496854
31	25.124961691353832	23.80017604225317	27.255154376861647	23.819707889531355
32	21.190347931544082	25.373024536316912	29.315895257940433	24.120732274198573
33	22.69214801209054	25.977120756297943	28.2152438346048	23.115487397006714
34	21.585423758822444	25.11126607141082	28.941610106946275	24.361700062820464
35	22.323243417252904	26.39908348087903	29.47926943579159	21.798403666076485
36	23.845262522379862	25.330913327505804	28.088667190721445	22.735156959392885
37	23.933980840635986	25.89417908065274	28.500789517584778	21.6710505611265
38	24.510026252186695	24.96503890484115	27.389850890420202	23.135083952551955
39	24.574119146842662	23.725141130227254	27.755753384786775	23.94498633814331
40	23.68570594859449	25.785959516601782	27.470924700470306	23.057409834333424
41	21.538631253559785	26.205184606869725	27.606873985920423	24.64931015365007
42	25.370484968895546	26.234757379212244	27.653867245758125	20.74089040613409
43	25.016683368180093	23.511918821651033	26.88929145032408	24.582106359844797
44	23.78352046485183	25.232385674769414	28.274212965877947	22.70988089450081
45	26.28335117513635	23.845550804471	27.30010682369472	22.57099119669793
46	22.066343138843173	24.770233042988497	30.654059402342842	22.509364415825484
47	24.767769336467595	24.654994568035825	28.572538487453485	22.00469760804309
48	24.72647509812788	26.07446646011088	27.47173706933184	21.7273213724294
49	23.18520307874482	25.575881586737715	30.06600828892836	21.172907045589106
50	23.675003347510376	26.36622189333294	26.520874506922365	23.437900252234314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1738.0
1	2153.5
2	2569.0
3	7759.5
4	12950.0
5	14584.0
6	16218.0
7	14181.0
8	12144.0
9	15930.0
10	19716.0
11	26858.5
12	34001.0
13	60783.5
14	87566.0
15	103078.0
16	118590.0
17	108482.5
18	98375.0
19	82156.0
20	65937.0
21	70578.5
22	75220.0
23	84791.0
24	94362.0
25	110448.5
26	126535.0
27	141518.5
28	156502.0
29	175806.0
30	195110.0
31	217175.0
32	239240.0
33	260173.5
34	281107.0
35	309417.0
36	337727.0
37	362167.5
38	386608.0
39	405720.0
40	424832.0
41	446806.5
42	468781.0
43	483989.0
44	499197.0
45	589281.0
46	679365.0
47	898494.5
48	1117624.0
49	1151488.5
50	1185353.0
51	968125.5
52	750898.0
53	615956.0
54	481014.0
55	507158.5
56	533303.0
57	505987.0
58	478671.0
59	467157.5
60	455644.0
61	384038.0
62	312432.0
63	281135.5
64	249839.0
65	208149.0
66	166459.0
67	149099.5
68	131740.0
69	112608.0
70	93476.0
71	74807.0
72	56138.0
73	50493.5
74	44849.0
75	34179.0
76	23509.0
77	20114.0
78	16719.0
79	14014.5
80	11310.0
81	9978.0
82	8646.0
83	8192.0
84	7738.0
85	6154.0
86	4570.0
87	4301.5
88	4033.0
89	2094.5
90	156.0
91	119.0
92	82.0
93	46.5
94	11.0
95	5.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044584877135168066
2	0.0
3	0.0
4	0.01665309502502033
5	0.015508194742050181
6	0.06929958407002779
7	0.07091758364348145
8	0.07722872817853177
9	0.0636223925842254
10	0.07540256491726534
11	0.07102166548738784
12	0.06730310506418727
13	0.0752890210875493
14	0.07301814449322834
15	0.06724633314932925
16	0.07057695215433331
17	0.0731316883229444
18	0.08510110037217775
19	0.06612035683797844
20	0.06740718690809365
21	0.06595004109340437
22	0.055693248475721394
23	0.05770865145318124
24	0.06287489570526142
25	0.0679086721560062
26	0.05734909599241375
27	0.061124428330472345
28	0.05246671131462371
29	0.06824930364515433
30	0.05934557499825426
31	0.06026338762179232
32	0.0557878683338181
33	0.06274242790392602
34	0.06397248605918321
35	0.06534447400158544
36	0.0576897274815619
37	0.061124428330472345
38	0.07173131442311313
39	0.06702870747570683
40	0.06195708308172337
41	0.07494838959840115
42	0.06409549187470892
43	0.053848161242835625
44	0.06586488322111733
45	0.01572582041567261
46	0.008913190632709745
47	0.014438990345557401
48	0.021686871475765108
49	0.11691229666429045
50	0.009944547085963843
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0568606E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.576399375963284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.75348548699795	21.709875162269427
2	16.15720817068981	10.203729159965572
3	6.316948447331937	5.983994610309732
4	3.074616459200544	3.8834126897450605
5	1.5968795957590793	2.5211853935507746
6	0.9223225183744935	1.7474174516182346
7	0.5903352337385035	1.3048462774360736
8	0.4192764465671518	1.059139242059129
9	0.2975298575121669	0.8455429446369869
>10	1.5586237219167653	8.97694603016888
>50	0.14454603370052854	3.1907143511162555
>100	0.13097092789510717	8.596419432485153
>500	0.019155602568613275	4.179268206421318
>1k	0.015575707388570281	9.812321617950772
>5k	0.001262895179433516	2.670608400760018
>10k+	0.001262895179433516	13.314579029506419
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	159535	1.5095179061458057	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	122457	1.158686396294838	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	86798	0.8212814443077924	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	74252	0.7025713703396645	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	70166	0.6639096963213502	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69201	0.654778880015018	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	59167	0.5598373144007829	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	58113	0.54986438135739	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47668	0.4510339395753802	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	41630	0.39390246925658884	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	39151	0.3704462064344153	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38265	0.3620628870070471	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	37728	0.35698180062725393	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	37073	0.35078419992191967	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	32446	0.30700359158057366	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	28788	0.2723916474887984	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	24597	0.23273646496046874	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	21645	0.20480468285032105	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	21480	0.20324345519172538	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	21448	0.20294067164581595	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	20092	0.19011021888790253	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	18946	0.17926678315001998	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	17895	0.16932223606405614	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	17843	0.16883021280195326	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	17648	0.16698512556906747	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAA	16687	0.157892157205974	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	15414	0.14584704927026326	No Hit
GTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCACTTTTTC	14260	0.13492791764590334	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	13790	0.13048078431535814	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	13618	0.1288533227560948	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	13572	0.12841807140884995	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13474	0.12749079679950223	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	13358	0.12639320644558044	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	13202	0.1249171366592718	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	12521	0.1184735243228861	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	12276	0.1161553377995168	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	12173	0.11518075326112072	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	11759	0.11126349113591708	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	10938	0.10349520078617748	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	10703	0.10127163412090487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.623390066769449E-5	0.0	0.0	0.0	9.461985809670642E-6
2	6.623390066769449E-5	0.0	0.0	0.0	9.461985809670642E-6
3	7.569588647736513E-5	0.0	0.0	0.0	1.8923971619341283E-5
4	7.569588647736513E-5	0.0	0.0	9.461985809670642E-6	1.8923971619341283E-5
5	1.0408184390637705E-4	0.0	0.0	9.461985809670642E-6	1.8923971619341283E-5
6	1.3246780133538899E-4	0.0	0.0	9.461985809670642E-6	1.8923971619341283E-5
7	1.3246780133538899E-4	0.0	0.0	1.8923971619341283E-5	1.8923971619341283E-5
8	1.3246780133538899E-4	0.0	0.0	1.8923971619341283E-5	1.8923971619341283E-5
9	1.419297871450596E-4	0.0	0.0	2.8385957429011923E-5	1.8923971619341283E-5
10	2.6493560267077797E-4	0.0	0.0	5.6771914858023846E-5	1.8923971619341283E-5
11	3.69017446577155E-4	0.0	0.0	7.569588647736513E-5	1.8923971619341283E-5
12	3.69017446577155E-4	0.0	0.0	1.0408184390637705E-4	1.8923971619341283E-5
13	3.879414181964963E-4	0.0	0.0	1.2300581552571833E-4	1.8923971619341283E-5
14	3.879414181964963E-4	0.0	0.0	1.3246780133538899E-4	1.8923971619341283E-5
15	3.879414181964963E-4	0.0	0.0	2.081636878127541E-4	1.8923971619341283E-5
16	4.920232621028733E-4	0.0	0.0	2.554736168611073E-4	8.515787228703577E-5
17	5.487951769608972E-4	0.0	0.0	3.5009347495781374E-4	8.515787228703577E-5
18	5.487951769608972E-4	0.0	0.0	3.9740340400616696E-4	1.5139177295473027E-4
19	5.582571627705679E-4	0.0	0.0	4.730992904835321E-4	1.7031574457407155E-4
20	5.677191485802384E-4	0.0	0.0	5.677191485802384E-4	1.7031574457407155E-4
21	6.33953049247933E-4	0.0	0.0	7.3803489315431E-4	1.797777303837422E-4
22	6.623390066769449E-4	0.0	0.0	0.0010408184390637706	1.797777303837422E-4
23	7.474968789639806E-4	0.0	0.0	0.0015896136160246678	1.8923971619341283E-4
24	7.569588647736513E-4	0.0	0.0	0.002185718722033918	1.8923971619341283E-4
25	7.758828363929926E-4	0.0	0.0	0.002327648509178978	1.8923971619341283E-4
26	8.799646802993697E-4	0.0	0.0	0.0026493560267077795	1.9870170200308348E-4
27	9.178126235380522E-4	0.0	0.0	0.0031224553171913115	1.9870170200308348E-4
28	9.556605667767348E-4	0.0	0.0	0.005090548365602805	1.9870170200308348E-4
29	0.001002970495825088	0.0	0.0	0.007541202690307502	2.081636878127541E-4
30	0.001002970495825088	0.0	0.0	0.011590932616846536	2.081636878127541E-4
31	0.0010786663823024532	0.0	0.0	0.0161799957345368	2.081636878127541E-4
32	0.0010786663823024532	0.0	0.0	0.021185386227852567	2.081636878127541E-4
33	0.0011165143255411358	0.0	0.0	0.027203209202803093	2.081636878127541E-4
34	0.001135438297160477	0.0	0.0	0.03484849373701697	2.2708765943209538E-4
35	0.0011543622687798182	0.0	0.0	0.045464841815467436	2.2708765943209538E-4
36	0.0012016721978281716	0.0	0.0	0.0573680199640331	2.2708765943209538E-4
37	0.0012489821268765247	9.461985809670642E-6	0.0	0.07543095087469436	2.3654964524176604E-4
38	0.0012584441126861953	9.461985809670642E-6	0.0	0.09636086348568582	2.4601163105143666E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTTAT	4690	0.0	19.757795	1
CGACGAG	910	0.0	18.12993	24
TGCGACG	945	0.0	17.457708	22
AATGCGA	990	0.0	17.330824	20
GTCGACG	1035	0.0	17.003174	36
CAATGCG	1085	0.0	16.624557	19
TCGACGA	1085	0.0	16.219618	37
GACGAGT	1045	0.0	16.208717	25
CGACGAA	1080	0.0	16.091103	38
TTATTGA	5250	0.0	16.00803	4
GTACTAG	1360	0.0	15.860484	1
ATTGAGC	4760	0.0	15.852673	6
AGTCGAC	1160	0.0	15.550357	35
CGTCGTA	2395	0.0	15.431743	10
CAATTCG	1180	0.0	15.287878	15
CCGTCGT	2415	0.0	15.121682	9
CATTGCG	990	0.0	15.109282	29
TATTGAG	5275	0.0	14.88906	5
TTTATTG	6480	0.0	14.870542	3
TTGAGCC	5035	0.0	14.855827	7
>>END_MODULE