##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9804226
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.841528642852584	33.0	32.0	34.0	27.0	34.0
2	31.889364443455303	33.0	32.0	34.0	27.0	34.0
3	31.8927458424561	33.0	32.0	34.0	27.0	34.0
4	31.793355538723812	33.0	32.0	34.0	28.0	34.0
5	31.841759257691532	33.0	32.0	34.0	28.0	34.0
6	35.31378550433252	38.0	36.0	38.0	29.0	38.0
7	35.37519483944985	38.0	36.0	38.0	29.0	38.0
8	35.427408446112935	38.0	37.0	38.0	29.0	38.0
9	35.59462603167246	38.0	37.0	38.0	29.0	38.0
10	35.52550543000539	38.0	37.0	38.0	29.0	38.0
11	35.58397083053777	38.0	37.0	38.0	29.0	38.0
12	35.48642677147589	38.0	37.0	38.0	29.0	38.0
13	35.54084626364182	38.0	37.0	38.0	29.0	38.0
14	35.559293308824174	38.0	37.0	38.0	29.0	38.0
15	35.54919807030152	38.0	37.0	38.0	29.0	38.0
16	35.51943223259031	38.0	37.0	38.0	29.0	38.0
17	35.46865525131714	38.0	37.0	38.0	28.0	38.0
18	35.472441475747296	38.0	37.0	38.0	28.0	38.0
19	35.48818142299045	38.0	37.0	38.0	28.0	38.0
20	35.414051858861676	38.0	37.0	38.0	28.0	38.0
21	35.43083798761881	38.0	37.0	38.0	28.0	38.0
22	35.467536142067715	38.0	37.0	38.0	28.0	38.0
23	35.473648914253914	38.0	37.0	38.0	28.0	38.0
24	35.47529657109087	38.0	37.0	38.0	28.0	38.0
25	35.489127647608285	38.0	37.0	38.0	28.0	38.0
26	35.489142539145874	38.0	37.0	38.0	28.0	38.0
27	35.48962610613015	38.0	37.0	38.0	28.0	38.0
28	35.50076538423329	38.0	37.0	38.0	28.0	38.0
29	35.466750970448864	38.0	37.0	38.0	28.0	38.0
30	35.474335352938624	38.0	37.0	38.0	28.0	38.0
31	35.51206275742726	38.0	37.0	38.0	28.0	38.0
32	35.51854537012917	38.0	37.0	38.0	29.0	38.0
33	35.490992659695934	38.0	37.0	38.0	28.0	38.0
34	35.46639377754042	38.0	37.0	38.0	28.0	38.0
35	35.46621048923189	38.0	37.0	38.0	28.0	38.0
36	35.44055165598998	38.0	37.0	38.0	28.0	38.0
37	35.430888374054206	38.0	37.0	38.0	28.0	38.0
38	35.42472562341994	38.0	37.0	38.0	28.0	38.0
39	35.42925622073584	38.0	37.0	38.0	28.0	38.0
40	35.418167635058595	38.0	37.0	38.0	28.0	38.0
41	35.411816496274156	38.0	37.0	38.0	28.0	38.0
42	35.31438463372835	38.0	37.0	38.0	27.0	38.0
43	35.361866607318106	38.0	37.0	38.0	28.0	38.0
44	35.34273087952073	38.0	37.0	38.0	28.0	38.0
45	35.35732907421759	38.0	37.0	38.0	28.0	38.0
46	35.317846916217555	38.0	37.0	38.0	27.0	38.0
47	35.25277609879658	38.0	37.0	38.0	27.0	38.0
48	35.20829018017333	38.0	37.0	38.0	27.0	38.0
49	35.12122711165573	38.0	37.0	38.0	27.0	38.0
50	34.81016186285383	38.0	36.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	16.0
4	514.0
5	1380.0
6	1968.0
7	968.0
8	250.0
9	188.0
10	160.0
11	117.0
12	132.0
13	116.0
14	125.0
15	215.0
16	527.0
17	1730.0
18	8760.0
19	25997.0
20	46433.0
21	62947.0
22	75689.0
23	88333.0
24	101471.0
25	114779.0
26	130600.0
27	147645.0
28	167981.0
29	191620.0
30	219566.0
31	251335.0
32	295804.0
33	358202.0
34	463355.0
35	664715.0
36	1324121.0
37	5056467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71683205718567	18.436700154296908	13.554005500239441	24.292462288277974
2	15.0823940614996	21.418172122919238	37.616860321253306	25.882573494327854
3	18.166441695652466	27.251421988844406	29.41577438137391	25.16636193412922
4	12.700644846431228	17.715893793552027	35.93691661147748	33.64654474853927
5	13.959924147900779	37.180586163428856	33.69849576053114	15.160993928139224
6	31.438770338220678	35.42742643518071	18.467146222077336	14.666657004521271
7	26.78124412478033	31.88174623215337	23.096469652583014	18.240539990483292
8	28.090937504401907	31.45489554737926	20.998691216246147	19.455475731972683
9	27.513251705190346	14.453357784150167	19.880828803492243	38.152561707167244
10	17.2031022317271	27.68497457812923	30.82218612982078	24.289737060322896
11	33.44325914412805	23.390497816946887	22.05022966533255	21.116013373592512
12	23.834900652531978	25.93271125026205	29.15955710211053	21.072830995095444
13	29.67264263933837	20.607153815171436	25.253344808812223	24.466858736677978
14	24.15957607730653	20.817426671830017	25.94731097211077	29.075686278752688
15	26.260828509237164	25.867242683430046	24.433215692034068	23.438713115298714
16	23.717994648023545	26.178478963311257	26.6500583516084	23.453468037056794
17	23.433828032499083	26.46898093332789	26.866002531335482	23.231188502837547
18	24.72460973746894	24.028904161636603	29.172258178715648	22.07422792217881
19	23.91395293747583	26.53985966709284	28.353089262110675	21.19309813332065
20	24.763794009351482	23.722428005295882	28.837696692034253	22.676081293318383
21	24.904219838460218	25.267468997111436	26.976457747624554	22.851853416803788
22	24.323750353353464	24.89303467609351	26.46661843030441	24.316596540248618
23	22.299194672296778	25.66397285203083	27.310336916649725	24.726495559022666
24	22.875252532726556	27.46982492254983	26.526236613022654	23.128685931700964
25	23.99062725306417	24.143304883316283	26.31862685460607	25.547441009013482
26	23.69861102649207	25.041517758415583	27.46991244557125	23.789958769521103
27	24.422357264071724	24.781465302041834	26.245769226215916	24.550408207670525
28	23.372188248620436	24.43299121618691	28.241548481903	23.953272053289655
29	23.685337001139658	24.024039563643726	28.959870304016803	23.330753131199806
30	23.830324220779154	25.090559132608124	28.596785041082523	22.482331605530195
31	25.248665053770353	24.051168125151936	26.62457664533488	24.075590175742832
32	22.271205958519612	25.043494482670788	27.95762482161343	24.72767473719617
33	23.184849874080303	25.403743176883193	27.503268931738013	23.908138017298498
34	22.434028160504262	24.94608149318441	28.03364318869334	24.58624715761799
35	23.311860695560778	25.843180444763963	28.460915339183273	22.384043520491982
36	24.13373592795751	25.24433416306307	26.97297916790194	23.648950741077478
37	24.22925018571885	25.632171250589252	27.49475858998799	22.643819973703906
38	24.335341899987835	24.90573999057706	26.93413169741098	23.824786412024128
39	24.503121866332577	23.991743065425233	26.957707450378017	24.547427617864166
40	24.17219090510693	25.662811657573627	26.755099387304003	23.40989805001544
41	22.433622808521758	25.93655838906445	27.067338706384785	24.562480096029006
42	25.55499331709623	26.12087646058393	26.885164688467277	21.43896553385256
43	25.16648649784986	23.561958428405422	26.388083121146412	24.883471952598306
44	23.677196188273985	25.22165802406538	27.34654585804252	23.754599929618117
45	25.647050422461216	24.22859066530309	26.7650411749854	23.35931773725029
46	22.882834324455498	24.758516615619573	29.122599319458235	23.236049740466694
47	24.80034553354135	24.77969839467475	27.66612556337157	22.75383050841233
48	24.9725416262417	26.009175575462834	26.55390523060851	22.46437756768696
49	23.5935886801928	25.544628177740137	28.58570518176771	22.276077960299354
50	24.125220997552248	26.10309043629905	25.875411444092773	23.896277122055935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1109.0
1	1442.5
2	1776.0
3	5088.0
4	8400.0
5	9568.5
6	10737.0
7	9581.0
8	8425.0
9	11116.5
10	13808.0
11	18846.0
12	23884.0
13	42905.0
14	61926.0
15	73806.0
16	85686.0
17	79387.0
18	73088.0
19	61277.5
20	49467.0
21	53980.5
22	58494.0
23	65552.5
24	72611.0
25	86169.5
26	99728.0
27	112879.5
28	126031.0
29	146697.5
30	167364.0
31	189313.0
32	211262.0
33	233221.5
34	255181.0
35	284557.5
36	313934.0
37	346677.0
38	379420.0
39	404537.5
40	429655.0
41	452314.5
42	474974.0
43	489999.0
44	505024.0
45	577883.5
46	650743.0
47	802229.5
48	953716.0
49	970366.5
50	987017.0
51	826414.0
52	665811.0
53	571705.5
54	477600.0
55	503342.0
56	529084.0
57	511758.5
58	494433.0
59	466401.0
60	438369.0
61	378644.5
62	318920.0
63	278627.5
64	238335.0
65	205060.0
66	171785.0
67	156115.0
68	140445.0
69	120630.0
70	100815.0
71	79448.5
72	58082.0
73	56422.0
74	54762.0
75	41895.0
76	29028.0
77	24422.0
78	19816.0
79	16534.5
80	13253.0
81	11790.5
82	10328.0
83	9719.5
84	9111.0
85	7265.5
86	5420.0
87	5238.5
88	5057.0
89	2627.0
90	197.0
91	149.0
92	101.0
93	56.5
94	12.0
95	6.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.043746441585495885
2	0.0
3	0.0
4	0.016135898948065865
5	0.014942536004371992
6	0.06670592864750365
7	0.0685418716378019
8	0.07490647400416922
9	0.06173868289041888
10	0.07292773544795887
11	0.06842967512172812
12	0.06519637552214728
13	0.07245855001710487
14	0.07107139309110173
15	0.06490058470704368
16	0.06858267037091964
17	0.07166297472130896
18	0.0829030256952461
19	0.06363582398039376
20	0.06550236602053033
21	0.06393161479549737
22	0.053609535316709346
23	0.05591466373786161
24	0.06110630252709393
25	0.06586955461858998
26	0.055149687491904006
27	0.05897456872169206
28	0.05049863191648173
29	0.06674672738062137
30	0.05768940862848327
31	0.05817899342589614
32	0.053874727081974653
33	0.06036172564769519
34	0.06227926610422893
35	0.06298304425050993
36	0.055618872922758
37	0.05929075890335453
38	0.0699494279303639
39	0.0652881726716622
40	0.06009653388242989
41	0.07329492404601853
42	0.06211607117175798
43	0.05184499010936713
44	0.06374802049646754
45	0.015819708766403387
46	0.008771727620313934
47	0.014432551840400253
48	0.02158252981928405
49	0.11204352082459135
50	0.009689699115463066
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9804226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.19161916063072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.63728158996567	23.113741299157418
2	15.479089691792014	10.275521000064106
3	5.984155499587888	5.9587143102094515
4	2.7653576242643667	3.671467884301177
5	1.5628908774697636	2.593743939730019
6	0.9648662621999485	1.9215284109529176
7	0.6180876476471712	1.4360730866016516
8	0.4826672241718728	1.2816405348825264
9	0.3356890226930406	1.0027855977868558
>10	1.8406068337225825	11.368312803367164
>50	0.1660387196064475	3.858710216638767
>100	0.13077222785399162	8.643030578943979
>500	0.015986771749473255	3.621141446157303
>1k	0.014164660466889429	9.61322603297114
>5k	0.00151213149512216	3.406688392546369
>10k+	8.332153136387412E-4	8.233674465689221
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	99758	1.0175000045898575	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	77902	0.7945757268345304	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	59790	0.6098390632774071	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	51295	0.5231927538186084	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49396	0.5038235552709617	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	45116	0.46016891083498074	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	37342	0.3808765730206546	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	37101	0.37841844935031077	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33031	0.3369057384030111	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27738	0.28291881480496267	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	26435	0.2696286274918591	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	26095	0.2661607351768513	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	24715	0.2520851722512313	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	23667	0.24139590417438356	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	21253	0.21677386873782795	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	19963	0.20361627730735707	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	16409	0.16736660293224576	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	15393	0.15700372472034	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	15267	0.1557185646271312	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	15080	0.15381122385387688	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	12809	0.13064774312628044	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	12451	0.12699625651224278	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	12233	0.12477272555732599	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	12077	0.12318157496573416	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	11315	0.11540941630680485	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	10161	0.10363898180233708	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	10052	0.10252721632487867	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	9981	0.10180303881203881	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.0998416397174033E-5	0.0	0.0	0.0	0.0
2	5.0998416397174033E-5	0.0	0.0	0.0	0.0
3	6.119809967660884E-5	0.0	0.0	0.0	0.0
4	6.119809967660884E-5	0.0	0.0	1.0199683279434807E-5	0.0
5	1.0199683279434807E-4	0.0	0.0	2.0399366558869615E-5	0.0
6	1.3259588263265249E-4	0.0	0.0	2.0399366558869615E-5	0.0
7	1.3259588263265249E-4	0.0	0.0	2.0399366558869615E-5	0.0
8	1.3259588263265249E-4	0.0	0.0	2.0399366558869615E-5	0.0
9	1.3259588263265249E-4	0.0	0.0	5.0998416397174033E-5	0.0
10	3.8758796461852265E-4	0.0	0.0	8.159746623547846E-5	0.0
11	4.3858638101569673E-4	1.0199683279434807E-5	0.0	9.179714951491326E-5	0.0
12	4.997844806923056E-4	2.0399366558869615E-5	0.0	1.0199683279434807E-4	0.0
13	4.997844806923056E-4	2.0399366558869615E-5	2.0399366558869615E-5	1.1219651607378288E-4	1.0199683279434807E-5
14	5.201838472511752E-4	2.0399366558869615E-5	2.0399366558869615E-5	1.2239619935321767E-4	1.0199683279434807E-5
15	5.303835305306099E-4	2.0399366558869615E-5	2.0399366558869615E-5	1.427955659120873E-4	1.0199683279434807E-5
16	5.81381946927784E-4	2.0399366558869615E-5	2.0399366558869615E-5	1.529952491915221E-4	1.0199683279434807E-5
17	6.527797298838277E-4	2.0399366558869615E-5	2.0399366558869615E-5	2.2439303214756576E-4	1.0199683279434807E-5
18	7.139778295604365E-4	2.0399366558869615E-5	2.0399366558869615E-5	2.3459271542700057E-4	1.0199683279434807E-5
19	7.547765626781757E-4	3.059904983830442E-5	2.0399366558869615E-5	2.6519176526530497E-4	1.0199683279434807E-5
20	7.751759292370453E-4	3.059904983830442E-5	2.0399366558869615E-5	3.059904983830442E-4	1.0199683279434807E-5
21	8.669730787519586E-4	3.059904983830442E-5	2.0399366558869615E-5	3.8758796461852265E-4	1.0199683279434807E-5
22	9.077718118696978E-4	3.059904983830442E-5	2.0399366558869615E-5	6.425800466043929E-4	1.0199683279434807E-5
23	9.995689613846112E-4	3.059904983830442E-5	2.0399366558869615E-5	0.0011831632604144375	1.0199683279434807E-5
24	0.0010709667443406547	3.059904983830442E-5	2.0399366558869615E-5	0.0014891537587974819	1.0199683279434807E-5
25	0.0010913661108995245	3.059904983830442E-5	2.0399366558869615E-5	0.0016013502748712647	1.0199683279434807E-5
26	0.0011423645272966984	3.059904983830442E-5	2.0399366558869615E-5	0.0018767417234160045	1.0199683279434807E-5
27	0.0012035626269733073	3.059904983830442E-5	2.0399366558869615E-5	0.002213331271637353	1.0199683279434807E-5
28	0.0012239619935321769	3.059904983830442E-5	2.0399366558869615E-5	0.003661686297317096	1.0199683279434807E-5
29	0.0012749604099293508	4.079873311773923E-5	2.0399366558869615E-5	0.0054364311879387525	1.0199683279434807E-5
30	0.0012851600932087858	4.079873311773923E-5	2.0399366558869615E-5	0.008057749790753497	1.0199683279434807E-5
31	0.001366757559444264	4.079873311773923E-5	2.0399366558869615E-5	0.011872431337262115	1.0199683279434807E-5
32	0.001366757559444264	4.079873311773923E-5	2.0399366558869615E-5	0.015748310983447342	1.0199683279434807E-5
33	0.001376957242723699	4.079873311773923E-5	2.0399366558869615E-5	0.020052577327368832	1.0199683279434807E-5
34	0.0014075562925620034	4.079873311773923E-5	2.0399366558869615E-5	0.0256114047146608	1.0199683279434807E-5
35	0.0014075562925620034	4.079873311773923E-5	2.0399366558869615E-5	0.03348556020638447	1.0199683279434807E-5
36	0.001427955659120873	4.079873311773923E-5	2.0399366558869615E-5	0.04323645742152415	1.0199683279434807E-5
37	0.001427955659120873	8.159746623547846E-5	2.0399366558869615E-5	0.05695503143236396	1.0199683279434807E-5
38	0.001427955659120873	8.159746623547846E-5	2.0399366558869615E-5	0.07135698422292591	1.0199683279434807E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	2140	0.0	15.8389435	1
TTAACGG	645	0.0	15.688934	35
AATGCGA	645	0.0	15.34646	20
CTAACGC	555	0.0	15.063344	3
CGTCGTA	2925	0.0	14.966886	10
GGCGTTA	3040	0.0	14.764036	42
TGCGACG	645	0.0	14.664395	22
GACCGTC	935	0.0	14.350719	22
ATACCGT	3375	0.0	14.340569	6
CCGTCGT	3145	0.0	14.33954	9
GGCGTCG	985	0.0	14.070827	8
TACCGTC	3235	0.0	14.009107	7
TAGGTCG	650	0.0	13.874987	21
GCGTCGG	1010	0.0	13.722118	9
GTCGTAG	3235	0.0	13.600658	11
GTCCTAT	2725	0.0	13.407902	1
GTACTAG	975	0.0	13.318847	1
GTCGACG	745	0.0	13.287481	36
GCATTCG	3710	0.0	13.280963	22
CGGGCGT	1035	0.0	13.178586	6
>>END_MODULE