FastQCFastQC Report
Wed 25 May 2016
SRR2558146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10554676
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA742890.7038491754744532No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT580850.5503248039068181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT544550.5159324644356682No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC419180.39715098786547315No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA367500.34818690786908096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353950.3353489960279217No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG302710.2868017928736041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287980.27284589313779034No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG278290.2636651281384668No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG251380.2381693194561349No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTT227370.21542110814202162No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC189110.17917177182890312No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA183620.17397028577665483No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC175800.1665612473561481No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT174660.1654811573562277No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC161510.1530222244624089No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC136150.12899495920102141No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT130990.12410613078032902No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC106370.10077997657152148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG13500.019.71641524
CAATGCG13050.019.55263519
TGCGACG13600.018.92382222
AATGCGA14100.018.56486720
GTCGACG13750.016.95835136
GACGAGT15900.016.87870225
GTACTAG18400.016.745521
GATCACT71050.016.6261938
AATTCGT14050.016.44116416
AGTCGAC15000.015.98549335
TCGACGA15350.015.47732437
CGACGAA14950.015.45007638
CATTGCG15050.015.347054529
CGTCGTA22100.015.32984410
TAGGTCG15550.015.27796821
CAATTCG14950.015.00992615
ACTGTCC77450.014.99859742
ACGCGAA6900.014.98390934
ATCGTCA17550.014.91691910
CGAACAA15550.014.85646941
Produced by FastQC (version 0.11.5)