FastQCFastQC Report
Wed 25 May 2016
SRR2558144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9327140
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA638300.6843469702395375No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT504690.5410983431148241No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444610.47668417113927747No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC393350.4217262740775844No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA344790.36966315505074443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293580.31475886498969674No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG286500.3071681136983041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245910.2636499505743454No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG240710.25807482250722086No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG239580.2568633042926342No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA174490.18707771085241565No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT165000.17690310212991336No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC160130.17168178026704864No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC159060.17053459045323646No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC136560.14641144016279373No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC132060.1415868101047052No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT110590.11856796402755829No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC103230.11067701353255124No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG101590.1089187039113812No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG99010.1061525826780771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13700.017.9917561
CAATGCG10900.017.55707619
CGACGAG10850.017.43599524
TGCGACG11350.016.08550322
AATGCGA11350.015.89204320
CATTGCG9500.015.05132629
AGGTCGT10450.014.9449522
GACGAGT12750.014.83753125
ACGCGAA5350.014.80237534
TCGACGA12400.014.36972237
GGCGTTA25150.014.25889642
GTCGACG12400.014.19231836
CGTCATT11700.014.10073726
CGACGAA12200.014.0644338
CGAACAA12800.013.92275941
TAACTCG2551.891749E-1013.8041325
CGTCGTA26300.013.71817810
CCGTCGT26300.013.634539
CGCGAAT5650.013.62757635
AATTCGT12850.013.525352516
Produced by FastQC (version 0.11.5)