##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558144_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9327140
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38878069804892	33.0	32.0	34.0	25.0	34.0
2	31.583443692278664	33.0	32.0	34.0	27.0	34.0
3	31.61961383661015	33.0	32.0	34.0	27.0	34.0
4	31.515566079205417	33.0	32.0	34.0	27.0	34.0
5	31.5843356055554	33.0	32.0	34.0	27.0	34.0
6	34.96961469432216	38.0	36.0	38.0	28.0	38.0
7	35.00617381105033	38.0	36.0	38.0	28.0	38.0
8	35.037173345741564	38.0	36.0	38.0	28.0	38.0
9	35.220021249815055	38.0	36.0	38.0	28.0	38.0
10	35.159943991405726	38.0	36.0	38.0	28.0	38.0
11	35.20267145127016	38.0	36.0	38.0	28.0	38.0
12	35.09831888445976	38.0	36.0	38.0	28.0	38.0
13	35.14723237777068	38.0	36.0	38.0	28.0	38.0
14	35.185225803408116	38.0	36.0	38.0	28.0	38.0
15	35.15360775114344	38.0	36.0	38.0	28.0	38.0
16	35.1203991791696	38.0	36.0	38.0	28.0	38.0
17	35.078873159403635	38.0	36.0	38.0	27.0	38.0
18	35.09920200618839	38.0	36.0	38.0	27.0	38.0
19	35.1085463496849	38.0	36.0	38.0	27.0	38.0
20	35.04771762834052	38.0	36.0	38.0	27.0	38.0
21	35.063144114916255	38.0	36.0	38.0	27.0	38.0
22	35.10168915659034	38.0	36.0	38.0	27.0	38.0
23	35.101482769637855	38.0	36.0	38.0	27.0	38.0
24	35.09324315921065	38.0	36.0	38.0	27.0	38.0
25	35.104253715501216	38.0	36.0	38.0	27.0	38.0
26	35.108202085526756	38.0	36.0	38.0	27.0	38.0
27	35.11908891685983	38.0	36.0	38.0	27.0	38.0
28	35.13591593993443	38.0	36.0	38.0	27.0	38.0
29	35.09886503258233	38.0	36.0	38.0	27.0	38.0
30	35.107042244460786	38.0	36.0	38.0	27.0	38.0
31	35.13619137270374	38.0	36.0	38.0	27.0	38.0
32	35.14945020660138	38.0	36.0	38.0	27.0	38.0
33	35.11918294353896	38.0	36.0	38.0	27.0	38.0
34	35.10126340979121	38.0	36.0	38.0	27.0	38.0
35	35.10555229148485	38.0	36.0	38.0	27.0	38.0
36	35.0841775721175	38.0	36.0	38.0	27.0	38.0
37	35.074259740928085	38.0	36.0	38.0	27.0	38.0
38	35.062636027764135	38.0	36.0	38.0	27.0	38.0
39	35.08075058378024	38.0	36.0	38.0	27.0	38.0
40	35.07297713983065	38.0	36.0	38.0	27.0	38.0
41	35.07133569347088	38.0	36.0	38.0	27.0	38.0
42	34.970202870333246	38.0	36.0	38.0	25.0	38.0
43	35.022008890184985	38.0	36.0	38.0	27.0	38.0
44	34.99585971691215	38.0	36.0	38.0	26.0	38.0
45	35.00875370156339	38.0	36.0	38.0	26.0	38.0
46	34.97754231200561	38.0	36.0	38.0	25.0	38.0
47	34.91828406135214	38.0	36.0	38.0	25.0	38.0
48	34.87572953767178	38.0	36.0	38.0	25.0	38.0
49	34.793995694285705	38.0	36.0	38.0	25.0	38.0
50	34.43659621277262	38.0	35.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	28.0
4	540.0
5	1271.0
6	1617.0
7	693.0
8	194.0
9	153.0
10	119.0
11	129.0
12	100.0
13	118.0
14	102.0
15	177.0
16	544.0
17	1812.0
18	9743.0
19	31159.0
20	58363.0
21	78441.0
22	91693.0
23	101822.0
24	113195.0
25	126179.0
26	139818.0
27	155551.0
28	175616.0
29	199314.0
30	228226.0
31	264958.0
32	311698.0
33	379609.0
34	493537.0
35	718772.0
36	1413328.0
37	4228521.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.15272243159383	19.060099520161454	13.751928535634361	24.03524951261035
2	15.038468383663158	21.58111704123665	38.45000718333809	24.930407391762106
3	17.8834884005172	27.25744440417963	30.345347019557977	24.513720175745192
4	12.705928272259168	17.823773137375586	36.77819215897369	32.69210643139155
5	13.7216112369206	37.49780314436389	34.20341505296741	14.577170565748096
6	30.558189526392677	36.208227722694744	18.828422168208984	14.405160582703601
7	26.11781484436443	32.28702312940846	23.5132125213071	18.08194950492001
8	27.17310233338783	32.18218517846192	21.301739280075633	19.342973208074614
9	27.058496283688143	14.75491715253286	20.427523631971685	37.759062931807314
10	16.885521587509515	27.77017309810144	31.518938656358774	23.825366658030266
11	32.83687769026416	23.482335109865605	23.106509437534914	20.57427776233532
12	23.177761466960835	25.98444016720566	29.67845758506984	21.15934078076367
13	28.67957077843078	21.012138104464515	26.298790051775228	24.00950106532948
14	23.564858939429115	21.05388508229159	26.613990578939895	28.767265399339404
15	25.40733133648962	26.600116652404264	24.945860261061515	23.0466917500446
16	23.475968259420778	26.739078917287806	26.450282429447885	23.334670393843528
17	22.71752072006504	26.87181142085565	27.213668087162564	23.19699977191675
18	23.486799377991442	24.916457701390186	29.51152285553129	22.085220065087082
19	23.492341249633522	26.460001752876394	29.132033322673756	20.915623674816324
20	24.536613990351555	24.148208858675073	29.207302093714567	22.10787505725881
21	24.375216226436347	25.620884322544136	27.301960721210705	22.70193872980881
22	23.832148071378086	25.490048851764186	27.134627301360243	23.543175775497488
23	22.044949000383053	26.007836292350532	27.75989456070949	24.187320146556925
24	22.899670898603752	27.252264549936488	27.314235890792705	22.53382866066706
25	23.59834291370542	24.68342826811053	27.143450691148136	24.574778127035916
26	23.31827448705492	25.429770305039252	27.76918688880646	23.48276831909937
27	23.839993855522394	25.08159025259295	27.120520581425485	23.957895310459172
28	22.73367236699729	25.148708008007752	28.649417224214435	23.468202400780523
29	23.11493233736858	24.838925443713347	29.130617387652247	22.915524831265827
30	23.046205279300793	25.931470158811106	28.652537943023486	22.369786618864612
31	24.549764354983783	24.811202403702527	27.17954879088121	23.459484450432484
32	22.07694066846129	25.687582433519	28.163527077667926	24.07194982035178
33	23.047918427935812	25.760404320350975	27.700994399016615	23.490682852696594
34	22.1666545984086	25.7314893035006	28.266660230262364	23.83519586782844
35	23.230193230936646	26.184080822574828	28.416191162034238	22.16953478445429
36	23.3875086283047	25.91299665379997	27.360052518016865	23.339442199878466
37	24.00655380300467	25.897894932977884	27.637051299255834	22.458499964761618
38	23.877158110608036	25.48676436069118	27.363059505985614	23.27301802271517
39	24.0350574749019	24.69002236818716	27.581537786852877	23.69338237005806
40	23.645801386527573	25.866869366970256	27.293441407265306	23.19388783923686
41	22.121639600443167	26.371922321435182	27.743541564679514	23.762896513442133
42	24.802943674684514	26.279147054362205	27.230095750311577	21.6878135206417
43	24.285767304324246	24.431001519703372	27.02804457682139	24.255186599150992
44	23.195291916617318	25.68934888391133	27.6986303133287	23.41672888614265
45	24.89070579447478	25.013838035048348	27.11727297741114	22.978183193065735
46	22.67442203329295	25.548566166067594	28.88778997248551	22.889221828153943
47	23.87270609406048	25.52008390440722	27.777615147400493	22.82959485413181
48	24.392616168591907	26.298776224526126	27.04562027358009	22.26298733330188
49	23.28900871448588	25.923135003963083	28.771552105799515	22.01630417575152
50	23.756693231760725	26.357547001795655	26.477239500476013	23.408520265967606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1199.0
1	1363.5
2	1528.0
3	4540.5
4	7553.0
5	8657.0
6	9761.0
7	8710.0
8	7659.0
9	9866.0
10	12073.0
11	17063.5
12	22054.0
13	39652.0
14	57250.0
15	69862.5
16	82475.0
17	77485.0
18	72495.0
19	64291.5
20	56088.0
21	63025.5
22	69963.0
23	79647.5
24	89332.0
25	103861.5
26	118391.0
27	126726.0
28	135061.0
29	153259.0
30	171457.0
31	209349.0
32	247241.0
33	266109.5
34	284978.0
35	311244.0
36	337510.0
37	364250.0
38	390990.0
39	420278.5
40	449567.0
41	477762.0
42	505957.0
43	509381.0
44	512805.0
45	568874.5
46	624944.0
47	720801.5
48	816659.0
49	829494.0
50	842329.0
51	744768.5
52	647208.0
53	556971.0
54	466734.0
55	473993.5
56	481253.0
57	456008.5
58	430764.0
59	403802.0
60	376840.0
61	324660.5
62	272481.0
63	239054.5
64	205628.0
65	174208.5
66	142789.0
67	129049.5
68	115310.0
69	98730.5
70	82151.0
71	66780.5
72	51410.0
73	48992.5
74	46575.0
75	36043.5
76	25512.0
77	21610.5
78	17709.0
79	14632.5
80	11556.0
81	10202.0
82	8848.0
83	8325.0
84	7802.0
85	6305.0
86	4808.0
87	4508.5
88	4209.0
89	2181.0
90	153.0
91	106.0
92	59.0
93	38.0
94	17.0
95	8.5
96	0.0
97	2.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04292848611685897
2	0.0
3	0.0
4	0.014152248170393067
5	0.014055755569231297
6	0.059578820517329004
7	0.06129424453798271
8	0.06724462160962524
9	0.054990061262080336
10	0.06566857579064965
11	0.06215195654830955
12	0.058388745103000496
13	0.0651861127848408
14	0.06321337516108903
15	0.05820648130080604
16	0.061079816535400996
17	0.06413541557219041
18	0.07516773630501955
19	0.05702712728660661
20	0.05821720270093512
21	0.056984241686090266
22	0.04767806637404392
23	0.04952214719624665
24	0.054561205256916906
25	0.05872110850700215
26	0.049339883394052195
27	0.05304948783871583
28	0.044729681338545366
29	0.059235735713198255
30	0.05153777042051476
31	0.052052397626710864
32	0.047988986977787404
33	0.053896478448913605
34	0.05537603166672742
35	0.056705485282734044
36	0.04958647559702116
37	0.052738567234972354
38	0.062098349547664126
39	0.05809926729951518
40	0.053639164845815546
41	0.06553991898910062
42	0.05560118106943822
43	0.04575893575093759
44	0.05682342068415398
45	0.014945631779945406
46	0.008373413500815899
47	0.013819884766391413
48	0.02037066024526275
49	0.10608825427730258
50	0.007944557495652474
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9327140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.287662842520795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.46866681905198	23.457372220345345
2	14.761840994250278	9.827743719030103
3	5.917757453949974	5.909649447327032
4	2.8452039840538936	3.788407637575317
5	1.5821147084931935	2.6332450497257245
6	0.9985681193161909	1.9943999328652464
7	0.6311255902340287	1.4706087101298098
8	0.46121079816453237	1.2282103638904691
9	0.3172837036006948	0.9505469655797606
>10	1.684654262857254	10.281239096677986
>50	0.15010053609842794	3.486936542812696
>100	0.13814566944658382	9.80338366332405
>500	0.023075887122782364	5.41182573123439
>1k	0.018531396301172776	12.073164995620044
>5k	0.0011034456605632562	2.4169621804562014
>10k+	6.16631398550055E-4	5.266303743406082
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	63830	0.6843469702395375	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	50469	0.5410983431148241	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44461	0.47668417113927747	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	39335	0.4217262740775844	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	34479	0.36966315505074443	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29358	0.31475886498969674	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	28650	0.3071681136983041	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24591	0.2636499505743454	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	24071	0.25807482250722086	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	23958	0.2568633042926342	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	17449	0.18707771085241565	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	16500	0.17690310212991336	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	16013	0.17168178026704864	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	15906	0.17053459045323646	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	13656	0.14641144016279373	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	13206	0.1415868101047052	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	11059	0.11856796402755829	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	10323	0.11067701353255124	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	10159	0.1089187039113812	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	9901	0.1061525826780771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.432840077451394E-5	0.0	0.0	0.0	0.0
2	6.432840077451394E-5	0.0	0.0	0.0	0.0
3	7.50498009035996E-5	0.0	0.0	0.0	0.0
4	7.50498009035996E-5	0.0	0.0	0.0	0.0
5	8.577120103268527E-5	0.0	0.0	0.0	0.0
6	1.1793540141994224E-4	0.0	0.0	0.0	0.0
7	1.1793540141994224E-4	0.0	0.0	0.0	0.0
8	1.3937820167811356E-4	0.0	0.0	1.0721400129085658E-5	0.0
9	1.500996018071992E-4	0.0	0.0	3.216420038725697E-5	0.0
10	4.39577405292512E-4	0.0	0.0	6.432840077451394E-5	0.0
11	5.575128067124542E-4	0.0	0.0	6.432840077451394E-5	0.0
12	6.003984072287968E-4	0.0	0.0	6.432840077451394E-5	0.0
13	6.111198073578825E-4	0.0	0.0	6.432840077451394E-5	0.0
14	6.111198073578825E-4	0.0	0.0	7.50498009035996E-5	0.0
15	6.218412074869682E-4	0.0	0.0	8.577120103268527E-5	1.0721400129085658E-5
16	6.968910083905677E-4	0.0	0.0	8.577120103268527E-5	2.1442800258171316E-5
17	7.397766089069104E-4	0.0	0.0	1.0721400129085658E-4	2.1442800258171316E-5
18	7.933836095523387E-4	0.0	0.0	1.0721400129085658E-4	2.1442800258171316E-5
19	8.1482640981051E-4	0.0	0.0	1.1793540141994224E-4	2.1442800258171316E-5
20	8.362692100686813E-4	0.0	0.0	1.3937820167811356E-4	3.216420038725697E-5
21	9.220404111013665E-4	0.0	0.0	1.8226380219445618E-4	3.216420038725697E-5
22	9.863688118758806E-4	0.0	0.0	2.4659220296897015E-4	3.216420038725697E-5
23	0.0010935828131667371	0.0	0.0	4.610202055506833E-4	3.216420038725697E-5
24	0.0011793540141994223	0.0	0.0	8.255478099395956E-4	3.216420038725697E-5
25	0.0012007968144575937	0.0	0.0	8.898762107141096E-4	3.216420038725697E-5
26	0.0013187322158775359	0.0	0.0	0.0010935828131667371	3.216420038725697E-5
27	0.0013616178163938786	0.0	0.0	0.0013616178163938786	3.216420038725697E-5
28	0.0013723392165229643	0.0	0.0	0.0026160216314969004	3.216420038725697E-5
29	0.0013723392165229643	0.0	0.0	0.004299281451763348	3.216420038725697E-5
30	0.00138306061665205	0.0	0.0	0.007247666487261904	3.216420038725697E-5
31	0.0013937820167811354	0.0	0.0	0.011193141734765426	3.216420038725697E-5
32	0.0013937820167811354	0.0	0.0	0.01510645278188169	3.216420038725697E-5
33	0.0014259462171683924	0.0	0.0	0.019051928029385212	3.216420038725697E-5
34	0.0014902746179429065	0.0	0.0	0.02393016508811919	5.360700064542829E-5
35	0.0015009960180719921	0.0	0.0	0.03111350317460658	5.360700064542829E-5
36	0.0015224388183301633	0.0	0.0	0.03967990187774602	5.360700064542829E-5
37	0.0015438816185883346	0.0	0.0	0.05070150121044607	5.360700064542829E-5
38	0.0015546030187174203	0.0	0.0	0.06397459457025412	5.360700064542829E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1370	0.0	17.991756	1
CAATGCG	1090	0.0	17.557076	19
CGACGAG	1085	0.0	17.435995	24
TGCGACG	1135	0.0	16.085503	22
AATGCGA	1135	0.0	15.892043	20
CATTGCG	950	0.0	15.051326	29
AGGTCGT	1045	0.0	14.94495	22
GACGAGT	1275	0.0	14.837531	25
ACGCGAA	535	0.0	14.802375	34
TCGACGA	1240	0.0	14.369722	37
GGCGTTA	2515	0.0	14.258896	42
GTCGACG	1240	0.0	14.192318	36
CGTCATT	1170	0.0	14.100737	26
CGACGAA	1220	0.0	14.06443	38
CGAACAA	1280	0.0	13.922759	41
TAACTCG	255	1.891749E-10	13.804132	5
CGTCGTA	2630	0.0	13.718178	10
CCGTCGT	2630	0.0	13.63453	9
CGCGAAT	565	0.0	13.627576	35
AATTCGT	1285	0.0	13.5253525	16
>>END_MODULE