FastQCFastQC Report
Wed 25 May 2016
SRR2558138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10127224
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA511550.5051236153164973No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT415280.4100630143067834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414940.4097272855819126No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC312600.3086729393958305No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA268850.26547255200437947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253890.25070048811006845No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTT232030.2291151059757343No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG220770.21799656055795746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209030.20640404517565722No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG190850.1884524327693354No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG172630.17046132286597No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA135840.13413349996010754No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC129150.12752754357956336No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT120830.119312064194492No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC117640.11616213880526391No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC108610.10724557884766843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACT67700.016.8640938
CGACGAA9500.016.2090938
GTCGACG9650.015.72909836
CGACGAG10000.015.61846224
TGCGACG9650.015.49983822
GTACTAG12450.015.3798071
AATGCGA10350.015.08933320
GCAGATC78350.014.90884635
ACTGTCC76000.014.87961642
TCGACGA10450.014.73546337
CAATGCG10750.014.73255919
AGATCAC81900.014.47727937
ATCAAGC79200.014.30301730
CCAGGAC92600.014.1362133
ATCACTG81400.014.08199139
CAAGCAG82700.014.01768932
AGTCGAC11000.013.99889835
GACAGGC85200.013.9437357
CGAACAA11500.013.77500741
TCAAGCA82950.013.73575431
Produced by FastQC (version 0.11.5)