##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10127224
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69494749992693	33.0	32.0	34.0	27.0	34.0
2	31.78213654600708	33.0	32.0	34.0	27.0	34.0
3	31.780496116211115	33.0	32.0	34.0	27.0	34.0
4	31.68318089932641	33.0	32.0	34.0	27.0	34.0
5	31.73691023324852	33.0	32.0	34.0	27.0	34.0
6	35.15393122537825	38.0	36.0	38.0	28.0	38.0
7	35.194966952444226	38.0	36.0	38.0	28.0	38.0
8	35.23315352756096	38.0	36.0	38.0	28.0	38.0
9	35.39986762413866	38.0	37.0	38.0	29.0	38.0
10	35.33724068905754	38.0	37.0	38.0	29.0	38.0
11	35.38903820039924	38.0	37.0	38.0	29.0	38.0
12	35.286450462634185	38.0	37.0	38.0	28.0	38.0
13	35.33807843096983	38.0	37.0	38.0	28.0	38.0
14	35.36642430344189	38.0	37.0	38.0	28.0	38.0
15	35.330811385232515	38.0	37.0	38.0	28.0	38.0
16	35.29957834447031	38.0	37.0	38.0	28.0	38.0
17	35.256299357059746	38.0	37.0	38.0	28.0	38.0
18	35.269561036667106	38.0	37.0	38.0	28.0	38.0
19	35.28191832233591	38.0	37.0	38.0	28.0	38.0
20	35.20596888150198	38.0	37.0	38.0	28.0	38.0
21	35.231992498635364	38.0	37.0	38.0	28.0	38.0
22	35.27068029698958	38.0	37.0	38.0	28.0	38.0
23	35.27476937411476	38.0	37.0	38.0	28.0	38.0
24	35.27334884663359	38.0	37.0	38.0	28.0	38.0
25	35.28238044305132	38.0	37.0	38.0	27.0	38.0
26	35.29215034643255	38.0	37.0	38.0	28.0	38.0
27	35.29185243656109	38.0	37.0	38.0	27.0	38.0
28	35.30182802315817	38.0	37.0	38.0	27.0	38.0
29	35.27226167802746	38.0	37.0	38.0	27.0	38.0
30	35.280338126222944	38.0	37.0	38.0	27.0	38.0
31	35.31732437240452	38.0	37.0	38.0	28.0	38.0
32	35.32699177978092	38.0	37.0	38.0	28.0	38.0
33	35.29769056159911	38.0	37.0	38.0	27.0	38.0
34	35.279930512053454	38.0	37.0	38.0	27.0	38.0
35	35.28985840542285	38.0	37.0	38.0	27.0	38.0
36	35.26962107286261	38.0	37.0	38.0	27.0	38.0
37	35.261004990113776	38.0	37.0	38.0	27.0	38.0
38	35.263363089431024	38.0	37.0	38.0	27.0	38.0
39	35.275255785790854	38.0	37.0	38.0	27.0	38.0
40	35.28402284772214	38.0	37.0	38.0	27.0	38.0
41	35.283280887240174	38.0	37.0	38.0	27.0	38.0
42	35.19346772620019	38.0	37.0	38.0	27.0	38.0
43	35.24884726554878	38.0	37.0	38.0	27.0	38.0
44	35.2289451679947	38.0	37.0	38.0	27.0	38.0
45	35.249766964767446	38.0	37.0	38.0	27.0	38.0
46	35.22567240538967	38.0	37.0	38.0	27.0	38.0
47	35.18037400969901	38.0	37.0	38.0	27.0	38.0
48	35.14509721518947	38.0	37.0	38.0	27.0	38.0
49	35.0667461290478	38.0	36.0	38.0	26.0	38.0
50	34.76036601935535	38.0	36.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	19.0
4	566.0
5	1508.0
6	2034.0
7	1058.0
8	228.0
9	186.0
10	163.0
11	116.0
12	115.0
13	133.0
14	118.0
15	233.0
16	555.0
17	1803.0
18	9723.0
19	32047.0
20	60693.0
21	81778.0
22	95882.0
23	107016.0
24	118217.0
25	130220.0
26	143609.0
27	158995.0
28	177681.0
29	200915.0
30	228107.0
31	261967.0
32	306853.0
33	370480.0
34	480019.0
35	690097.0
36	1367153.0
37	5096937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.42053224547084	18.988085171287764	13.677395926521866	24.91398665671953
2	14.968159092758293	20.80459561277602	39.38749651434589	24.839748780119802
3	17.79459010682493	26.89399385260956	29.854351004776824	25.457065035788684
4	12.456265774911659	17.312309104866443	37.627121456190814	32.60430366403108
5	13.64167146139596	37.431745473054605	34.455378994957584	14.471204070591847
6	30.90809399900913	36.66685045455884	18.563110687147358	13.861944859284678
7	26.612764727958382	31.653117072093913	23.75155951923824	17.98255868070947
8	26.59502266914901	33.00406740267287	21.315492495661832	19.085417432516294
9	27.027008868052643	14.547866853528923	20.56059862319511	37.86452565522333
10	16.663796027925336	27.70232592886409	31.91792562209607	23.7159524211145
11	33.3051487142962	23.444056363836324	23.327615712650086	19.923179209217388
12	23.429487645031084	25.421483497411145	29.924994834742897	21.224034022814877
13	28.37015525822384	20.98572044902926	26.172341984306787	24.47178230844012
14	23.206570228142365	20.565263494800814	27.08032339675886	29.147842880297965
15	24.930924985171217	26.870390737866305	24.61304603054448	23.585638246417993
16	23.247964779999997	26.633978774444156	26.26236405969511	23.855692385860735
17	22.37163320104266	26.854133845565663	27.318615316554425	23.45561763683725
18	23.37482050719503	25.409984790671615	28.989553098740068	22.225641603393292
19	23.537429780533568	26.108722306829712	28.515429583114265	21.838418329522455
20	24.311524703019423	25.034744032449964	28.184438171333014	22.4692930931976
21	24.932350995818243	25.42150077337144	27.123925782427204	22.522222448383115
22	24.173536510990154	25.433034590898263	27.000723498567574	23.392705399544006
23	22.600975333887206	25.969788736937776	27.53073989248781	23.89849603668721
24	23.209826408547073	26.740108824421565	27.102596708392245	22.94746805863911
25	23.548082762360472	25.054725849038668	27.017297459025023	24.37989392957583
26	23.519430058340415	25.54739895649411	27.677477659589915	23.255693325575557
27	24.045202596555708	25.216458685320763	26.82880319332879	23.909535524794737
28	23.051751713568944	25.02317970069758	28.349369191058333	23.575699394675144
29	23.189213752976794	24.732121921641394	28.755453481982723	23.32321084339909
30	23.30117768573199	25.6548497617547	28.317148474438685	22.726824078074625
31	24.21758274808023	24.887845013793736	27.3689486548219	23.525623583304135
32	22.579770953299498	25.411456524084524	27.816045757174457	24.192726765441524
33	23.246826644429053	25.988999099890425	27.31082631244572	23.4533479432348
34	22.743905855068476	25.761803316153898	27.844282593279736	23.65000823549789
35	23.640709356646564	25.90520239666743	28.12308808068991	22.331000165996095
36	23.70810251120642	25.79048612696032	26.942764016547073	23.55864734528619
37	23.970149023680882	25.961804851579025	27.3362733369456	22.731772787794498
38	23.98497681196282	25.44685130733534	27.218426636366537	23.349745244335303
39	23.749317844765923	25.234215276186532	27.3182631248248	23.698203754222742
40	23.833229786416698	25.52007624530976	27.47625337219109	23.170440596082447
41	22.45586467062933	25.88894189836463	27.646864794742655	24.008328636263382
42	24.291707469669095	26.25411492371687	27.01100300243568	22.443174604178353
43	24.224890217068282	24.50317098000038	27.193771616725577	24.07816718620576
44	23.381446623810955	25.747624134354524	27.63424637623736	23.23668286559716
45	24.623115329750878	25.009826535477647	26.917527418502964	23.449530716268516
46	23.026364061268833	25.47813258573719	28.4386445109444	23.05685884204958
47	23.76048859128062	25.6687199109124	27.43002898349201	23.140762514314968
48	24.42605184528291	26.06266988125633	26.642279715719972	22.868998557740788
49	23.338019637002912	25.800513720635145	28.11023008689993	22.751236555462018
50	23.68797169753094	26.115584874078206	26.766433034689285	23.430010393701565
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1669.0
1	1752.0
2	1835.0
3	4578.0
4	7321.0
5	8198.0
6	9075.0
7	7983.0
8	6891.0
9	8950.5
10	11010.0
11	15187.5
12	19365.0
13	34336.5
14	49308.0
15	60917.0
16	72526.0
17	67608.0
18	62690.0
19	55722.5
20	48755.0
21	56903.0
22	65051.0
23	80095.5
24	95140.0
25	115859.0
26	136578.0
27	156463.5
28	176349.0
29	205483.0
30	234617.0
31	262075.0
32	289533.0
33	315984.0
34	342435.0
35	375306.0
36	408177.0
37	436976.5
38	465776.0
39	487017.5
40	508259.0
41	527681.0
42	547103.0
43	556859.5
44	566616.0
45	608161.5
46	649707.0
47	713723.5
48	777740.0
49	782897.5
50	788055.0
51	697730.5
52	607406.0
53	569651.0
54	531896.0
55	530847.0
56	529798.0
57	499961.5
58	470125.0
59	441637.0
60	413149.0
61	365156.5
62	317164.0
63	279063.5
64	240963.0
65	206203.0
66	171443.0
67	153308.5
68	135174.0
69	123910.5
70	112647.0
71	92842.0
72	73037.0
73	70165.0
74	67293.0
75	61105.5
76	54918.0
77	40971.5
78	27025.0
79	18930.0
80	10835.0
81	9571.0
82	8307.0
83	7572.5
84	6838.0
85	5348.5
86	3859.0
87	3685.5
88	3512.0
89	1837.5
90	163.0
91	121.0
92	79.0
93	44.5
94	10.0
95	5.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0432102617657119
2	0.0
3	0.0
4	0.015532390712400554
5	0.015028797625094497
6	0.06775795617831698
7	0.07028579598910817
8	0.07646715427643351
9	0.06319599526977976
10	0.07430466631329571
11	0.06958471541658405
12	0.06636566940752965
13	0.07344559575259715
14	0.07179657525102634
15	0.0662076794193552
16	0.0697624541532803
17	0.07244828395224595
18	0.08328047251645664
19	0.06549672447257017
20	0.06695813186318383
21	0.06519061887048218
22	0.055029887756012905
23	0.0569455163626281
24	0.06254428656856015
25	0.06692850874040113
26	0.0569455163626281
27	0.0604706679737705
28	0.05155410801617502
29	0.06768883555849066
30	0.05858466248993801
31	0.05887101934350421
32	0.05549398334627535
33	0.06155684914246984
34	0.06322561839256247
35	0.06422293019291368
36	0.05721212446767248
37	0.06070765295603217
38	0.07045366035154352
39	0.06642491565309507
40	0.06130011541168637
41	0.07346534450111897
42	0.06360084461447678
43	0.05363760098522557
44	0.06514124699917766
45	0.015601511332226876
46	0.008896811209073681
47	0.014416586420918507
48	0.021101537795549897
49	0.11079047920733262
50	0.008877062460551874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0127224E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.18434007160526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88973788553167	27.450621990797057
2	14.649360835529501	11.187523519510277
3	5.62294508638615	6.441253421475918
4	2.6036770775051883	3.9767876385640584
5	1.4122642577819047	2.6963189345058725
6	0.827040346745881	1.8947993911849832
7	0.5796402883943258	1.54932273238766
8	0.36902399006073633	1.1272750024847868
9	0.2826992552978987	0.9715216052056066
>10	1.4664706783364627	10.227087766578896
>50	0.14141858127764875	3.792196772194355
>100	0.1261353809495399	9.966857093250068
>500	0.016351984218774138	4.322354899170689
>1k	0.012196363681686619	9.029157283663627
>5k	6.227929815756537E-4	1.5872488650102097
>10k+	4.151953210504358E-4	3.779673084016054
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	51155	0.5051236153164973	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	41528	0.4100630143067834	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41494	0.4097272855819126	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	31260	0.3086729393958305	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	26885	0.26547255200437947	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25389	0.25070048811006845	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTT	23203	0.2291151059757343	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	22077	0.21799656055795746	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20903	0.20640404517565722	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	19085	0.1884524327693354	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	17263	0.17046132286597	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	13584	0.13413349996010754	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	12915	0.12752754357956336	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	12083	0.119312064194492	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	11764	0.11616213880526391	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	10861	0.10724557884766843	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.962312278270926E-5	0.0	0.0	0.0	9.874374260903087E-6
2	2.962312278270926E-5	0.0	0.0	0.0	9.874374260903087E-6
3	2.962312278270926E-5	0.0	0.0	0.0	1.9748748521806174E-5
4	2.962312278270926E-5	0.0	0.0	0.0	1.9748748521806174E-5
5	1.2836686539174013E-4	0.0	0.0	0.0	1.9748748521806174E-5
6	1.481156139135463E-4	0.0	0.0	0.0	3.949749704361235E-5
7	1.579899881744494E-4	0.0	0.0	0.0	3.949749704361235E-5
8	1.6786436243535247E-4	0.0	0.0	0.0	6.91206198263216E-5
9	1.6786436243535247E-4	0.0	0.0	0.0	6.91206198263216E-5
10	2.468593565225772E-4	0.0	0.0	1.9748748521806174E-5	8.886936834812778E-5
11	3.752262219143173E-4	0.0	0.0	2.962312278270926E-5	8.886936834812778E-5
12	3.752262219143173E-4	0.0	0.0	3.949749704361235E-5	8.886936834812778E-5
13	3.851005961752204E-4	0.0	0.0	3.949749704361235E-5	8.886936834812778E-5
14	3.9497497043612347E-4	0.0	0.0	5.924624556541852E-5	8.886936834812778E-5
15	4.0484934469702655E-4	0.0	0.0	7.89949940872247E-5	9.874374260903087E-5
16	4.1472371895792963E-4	0.0	0.0	9.874374260903087E-5	2.27110608000771E-4
17	6.220855784368945E-4	0.0	0.0	1.1849249113083704E-4	2.27110608000771E-4
18	6.615830754805068E-4	0.0	0.0	1.2836686539174013E-4	2.468593565225772E-4
19	7.010805725241191E-4	0.0	0.0	1.481156139135463E-4	2.468593565225772E-4
20	7.307036953068284E-4	0.0	0.0	1.6786436243535247E-4	2.764824793052864E-4
21	7.800755666113439E-4	0.0	9.874374260903087E-6	1.9748748521806174E-4	3.554774733925111E-4
22	7.899499408722469E-4	0.0	9.874374260903087E-6	2.3698498226167408E-4	3.554774733925111E-4
23	8.294474379158593E-4	0.0	9.874374260903087E-6	3.159799763488988E-4	3.653518476534142E-4
24	8.491961864376654E-4	0.0	9.874374260903087E-6	5.233418358278636E-4	3.653518476534142E-4
25	8.491961864376654E-4	0.0	9.874374260903087E-6	5.628393328714759E-4	3.653518476534142E-4
26	8.886936834812778E-4	0.0	9.874374260903087E-6	6.91206198263216E-4	3.851005961752204E-4
27	9.183168062639871E-4	0.0	9.874374260903087E-6	8.985680577421809E-4	4.1472371895792963E-4
28	9.676886775685025E-4	0.0	9.874374260903087E-6	0.0016490205015708154	4.443468417406389E-4
29	0.0010664324201775334	0.0	9.874374260903087E-6	0.002468593565225772	4.54221216001542E-4
30	0.0012145480340910795	0.0	9.874374260903087E-6	0.004216357809405618	4.54221216001542E-4
31	0.001244171156873789	0.0	9.874374260903087E-6	0.0068133182400231295	4.640955902624451E-4
32	0.0012737942796564982	0.0	9.874374260903087E-6	0.009232539933944387	4.640955902624451E-4
33	0.0012737942796564982	0.0	9.874374260903087E-6	0.012461460317259695	4.7396996452334815E-4
34	0.0013034174024392075	0.0	9.874374260903087E-6	0.016164350665098352	5.035930873060574E-4
35	0.0013034174024392075	0.0	9.874374260903087E-6	0.021782869619552208	5.035930873060574E-4
36	0.0013231661509610136	0.0	9.874374260903087E-6	0.02856656473679263	5.035930873060574E-4
37	0.0013527892737437229	0.0	9.874374260903087E-6	0.03813483339560772	5.035930873060574E-4
38	0.001362663648004626	0.0	9.874374260903087E-6	0.04931262505895002	5.430905843496698E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCACT	6770	0.0	16.86409	38
CGACGAA	950	0.0	16.20909	38
GTCGACG	965	0.0	15.729098	36
CGACGAG	1000	0.0	15.618462	24
TGCGACG	965	0.0	15.499838	22
GTACTAG	1245	0.0	15.379807	1
AATGCGA	1035	0.0	15.089333	20
GCAGATC	7835	0.0	14.908846	35
ACTGTCC	7600	0.0	14.879616	42
TCGACGA	1045	0.0	14.735463	37
CAATGCG	1075	0.0	14.732559	19
AGATCAC	8190	0.0	14.477279	37
ATCAAGC	7920	0.0	14.303017	30
CCAGGAC	9260	0.0	14.136213	3
ATCACTG	8140	0.0	14.081991	39
CAAGCAG	8270	0.0	14.017689	32
AGTCGAC	1100	0.0	13.998898	35
GACAGGC	8520	0.0	13.943735	7
CGAACAA	1150	0.0	13.775007	41
TCAAGCA	8295	0.0	13.735754	31
>>END_MODULE