FastQCFastQC Report
Wed 25 May 2016
SRR2558137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9233363
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA362000.3920565020567262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301330.32634913194683235No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT279990.30323729284768725No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG228790.24778620747391822No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC217160.23519058007358748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186190.20164917159652446No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA183990.19926650777187033No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG157170.170219669691314No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCAGCTATGG152140.16477203376494567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151790.1643929736110234No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCACAGCCAT149380.16178287369401592No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTTGCAGACA138710.15022695414444337No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG135000.14620891651286752No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG124620.13496707537654482No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA95650.10359172492189467No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA93510.10127404283791291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC43550.019.0954573
CTAGACA45200.018.5934834
TAGACAT46550.018.3370325
GTTCTAG46750.018.267891
CTCTAGT43650.018.24291627
CTCTACC87350.017.966561
TCTACCA90750.017.3577252
ACTTGCA49350.016.49582140
GTAGACC71150.016.3884094
CCTTACC93750.016.35401528
GGTATAG57500.016.3455051
AATCTAC65250.016.31847214
CTTACCT95150.016.1371229
GAATCTA66400.016.13533213
ATCCTTA96100.016.11432526
TAGACCT74200.016.099525
GACATGT37700.015.8139457
CCTCTAG63550.015.78382126
TATTGGT40500.015.7535644
TTACCTT98800.015.69647330
Produced by FastQC (version 0.11.5)