##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558137_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9233363
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.651195236231914	33.0	32.0	34.0	27.0	34.0
2	31.71693282285122	33.0	32.0	34.0	27.0	34.0
3	31.73057021585743	33.0	32.0	34.0	27.0	34.0
4	31.624043265709364	33.0	32.0	34.0	27.0	34.0
5	31.67616977692743	33.0	32.0	34.0	27.0	34.0
6	35.13594559208817	38.0	36.0	38.0	28.0	38.0
7	35.184301646106626	38.0	36.0	38.0	28.0	38.0
8	35.225727938996876	38.0	36.0	38.0	28.0	38.0
9	35.415958194213744	38.0	37.0	38.0	29.0	38.0
10	35.34514109322898	38.0	37.0	38.0	29.0	38.0
11	35.41098546650879	38.0	37.0	38.0	29.0	38.0
12	35.30129119801745	38.0	37.0	38.0	28.0	38.0
13	35.34643704574379	38.0	37.0	38.0	28.0	38.0
14	35.369771988819245	38.0	37.0	38.0	28.0	38.0
15	35.333278568166335	38.0	37.0	38.0	28.0	38.0
16	35.31335050945143	38.0	37.0	38.0	28.0	38.0
17	35.26684740976825	38.0	37.0	38.0	28.0	38.0
18	35.28257569858349	38.0	37.0	38.0	28.0	38.0
19	35.28772983364783	38.0	37.0	38.0	28.0	38.0
20	35.2197865501443	38.0	36.0	38.0	28.0	38.0
21	35.26168255271671	38.0	37.0	38.0	28.0	38.0
22	35.302794550587905	38.0	37.0	38.0	28.0	38.0
23	35.30607309601063	38.0	37.0	38.0	28.0	38.0
24	35.31388628390327	38.0	37.0	38.0	28.0	38.0
25	35.313432169838876	38.0	37.0	38.0	28.0	38.0
26	35.30634818537948	38.0	37.0	38.0	28.0	38.0
27	35.321396223672785	38.0	37.0	38.0	28.0	38.0
28	35.329750492859425	38.0	37.0	38.0	28.0	38.0
29	35.29505067655198	38.0	37.0	38.0	28.0	38.0
30	35.32661772314161	38.0	37.0	38.0	28.0	38.0
31	35.35881043559102	38.0	37.0	38.0	28.0	38.0
32	35.376565396594934	38.0	37.0	38.0	28.0	38.0
33	35.34586130752143	38.0	37.0	38.0	28.0	38.0
34	35.32510364858395	38.0	37.0	38.0	28.0	38.0
35	35.34754314327294	38.0	37.0	38.0	28.0	38.0
36	35.3371024186962	38.0	37.0	38.0	28.0	38.0
37	35.33474791362584	38.0	37.0	38.0	28.0	38.0
38	35.33096294383747	38.0	37.0	38.0	28.0	38.0
39	35.344107775249384	38.0	37.0	38.0	28.0	38.0
40	35.34253315936999	38.0	37.0	38.0	28.0	38.0
41	35.350166456143874	38.0	37.0	38.0	28.0	38.0
42	35.261156200617265	38.0	37.0	38.0	27.0	38.0
43	35.31330794641129	38.0	37.0	38.0	28.0	38.0
44	35.29952401957986	38.0	37.0	38.0	27.0	38.0
45	35.33517040324311	38.0	37.0	38.0	28.0	38.0
46	35.3194456884236	38.0	37.0	38.0	27.0	38.0
47	35.284290350114034	38.0	37.0	38.0	27.0	38.0
48	35.260966129025796	38.0	37.0	38.0	27.0	38.0
49	35.18880044031628	38.0	37.0	38.0	27.0	38.0
50	34.87284308003487	38.0	36.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	21.0
4	464.0
5	1272.0
6	1778.0
7	790.0
8	227.0
9	150.0
10	147.0
11	131.0
12	107.0
13	130.0
14	114.0
15	189.0
16	415.0
17	1444.0
18	7273.0
19	24237.0
20	47247.0
21	67459.0
22	82148.0
23	94735.0
24	107242.0
25	120152.0
26	132637.0
27	147513.0
28	164405.0
29	185074.0
30	211184.0
31	241749.0
32	284243.0
33	342699.0
34	441301.0
35	633277.0
36	1262787.0
37	4628622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5274623664349	17.852320531553858	12.853379105930465	25.76683799608078
2	16.223796248452487	20.498370961912794	37.667510743377036	25.610322046257682
3	18.295489953118924	24.72885556432689	29.965549930182533	27.010104552371654
4	13.505486970678746	16.310476642327068	35.94491690199277	34.23911948500141
5	14.954691615072942	36.07058268430923	33.48655653025011	15.488169170367716
6	32.88002762186054	35.17968189344126	17.491905151940824	14.448385332757372
7	28.99656853230438	29.964105220116032	22.306230666633287	18.733095580946298
8	27.23808289301872	31.93792474391811	20.920128201348774	19.90386416171439
9	27.623915678213695	13.773520008045178	19.904532868192593	38.69803144554854
10	16.970347508742694	26.976138219263852	30.469325003438335	25.584189268555118
11	34.46727566539944	22.586923531178265	22.77198354400762	20.173817259414665
12	24.7135245333581	24.262149367672713	29.065120981353832	21.959205117615358
13	29.689760366717778	20.04770383048233	25.35747526913605	24.905060533663846
14	24.71335155291458	19.829471308819734	25.386176260305408	30.07100087796027
15	25.362053883379833	26.787500845287287	23.282300377821745	24.56814489351114
16	25.322656751738158	24.997296066519148	24.916579046216995	24.7634681355257
17	23.387223418772166	26.216087129596204	26.145923411001487	24.250766040630147
18	24.998997392352997	23.915975192560282	27.83096837157658	23.254059043510136
19	25.14817304895552	24.7790571109409	26.88268526453544	23.190084575568147
20	25.638028213278254	23.750532372011264	26.764478622215815	23.846960792494663
21	25.752396875378953	24.177049577561924	26.674886806015298	23.395666741043826
22	25.198761422578293	23.747444372157496	25.919050799618404	25.13474340564581
23	23.68050117971936	24.242425555883283	26.145396212111528	25.931677052285828
24	23.77896096665132	25.412893676309046	26.286985202938755	24.52116015410088
25	25.579889742975638	23.869865500301817	25.551366400325982	24.998878356396563
26	25.426395934685896	24.26827856971958	25.927105041419463	24.37822045417506
27	25.293490983066246	23.839958162736423	25.917463080576287	24.949087773621045
28	23.77850438165543	23.93568399897193	26.813232560387267	25.472579058985374
29	24.244848824546132	23.710031918869955	26.62392249565617	25.42119676092774
30	24.2881426882058	24.401088869236755	27.679117981675695	23.63165046088175
31	25.834311668085856	23.692229937888655	26.075342865062623	24.39811552896286
32	24.416396032917934	24.020541689372234	26.505738056727058	25.057324220982775
33	24.507775996005208	24.192854497186502	26.124039256984222	25.175330249824068
34	24.423131106468198	23.424975509107142	25.98756816270622	26.16432522171844
35	24.432723059357368	24.34237618457681	27.09730023554992	24.127600520515905
36	24.836779570526904	23.620565096861657	26.90223548407965	24.64041984853179
37	25.548698487555953	24.18443213182331	26.521216153686943	23.745653226933793
38	25.210343132818945	23.438296728633922	27.045529053552087	24.305831084995045
39	25.829995254460712	23.388237022944068	26.243894276883957	24.53787344571126
40	25.47583367134201	24.75164083659124	25.484958009082508	24.287567482984247
41	25.177256347777384	24.07384025670185	26.259170071479115	24.48973332404165
42	26.131080964219848	24.321537813667184	25.457034755859816	24.090346466253152
43	25.28557927337355	23.699270955031356	25.485042127967862	25.530107643627233
44	25.061092978582618	24.486802724568037	25.45002903203364	25.00207526481571
45	25.322257068435817	23.912279829501603	25.370199045861497	25.395264056201082
46	24.18726368402771	24.365458206983142	26.84525616654104	24.602021942448108
47	25.363898866758127	24.106514033971475	25.92459482124215	24.604992278028245
48	25.455556418541576	24.67480682743988	25.174139927008714	24.69549682700983
49	24.0517607578498	24.89955393686334	26.86109549255271	24.18758981273415
50	25.458411403689386	24.622069796008507	25.354042001860584	24.565476798441523
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	714.0
1	1018.0
2	1322.0
3	3571.0
4	5820.0
5	6470.5
6	7121.0
7	6192.0
8	5263.0
9	6595.0
10	7927.0
11	10808.0
12	13689.0
13	25110.5
14	36532.0
15	44450.0
16	52368.0
17	47220.0
18	42072.0
19	35826.0
20	29580.0
21	32962.5
22	36345.0
23	41153.5
24	45962.0
25	55965.0
26	65968.0
27	75624.0
28	85280.0
29	102541.0
30	119802.0
31	137118.5
32	154435.0
33	172826.5
34	191218.0
35	223982.0
36	256746.0
37	284810.0
38	312874.0
39	359039.0
40	405204.0
41	423815.0
42	442426.0
43	461590.0
44	480754.0
45	536716.0
46	592678.0
47	691447.0
48	790216.0
49	812427.5
50	834639.0
51	748915.0
52	663191.0
53	604834.0
54	546477.0
55	565507.0
56	584537.0
57	566152.0
58	547767.0
59	519560.5
60	491354.0
61	441836.0
62	392318.0
63	347404.0
64	302490.0
65	258647.5
66	214805.0
67	187395.5
68	159986.0
69	136812.5
70	113639.0
71	94682.0
72	75725.0
73	62153.5
74	48582.0
75	37228.5
76	25875.0
77	20206.0
78	14537.0
79	12284.0
80	10031.0
81	9199.0
82	8367.0
83	7942.0
84	7517.0
85	6136.0
86	4755.0
87	4501.5
88	4248.0
89	2183.5
90	119.0
91	95.5
92	72.0
93	43.5
94	15.0
95	7.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042985421454783054
2	0.0
3	0.0
4	0.01616962313731194
5	0.014046886275347347
6	0.06377957846994643
7	0.06623805432538502
8	0.07177233257264985
9	0.05929583836355183
10	0.07057017037021072
11	0.06591314562202309
12	0.06264239800817968
13	0.06964959571068527
14	0.06816584596533246
15	0.06206839263224028
16	0.06568570952966975
17	0.06901060859407347
18	0.08037158292162888
19	0.06107200594193037
20	0.06273987061918827
21	0.06086623042980114
22	0.05102149671793473
23	0.05303593067877869
24	0.058288621383129846
25	0.06317308222367084
26	0.05253773733362373
27	0.056599096125647834
28	0.047989015486556735
29	0.06396369340185153
30	0.05495289202861406
31	0.055201988701191536
32	0.05107564816849505
33	0.057931221809431735
34	0.058949269079965776
35	0.060595473176999544
36	0.05298177922821837
37	0.05689151395867356
38	0.066909532312333
39	0.0622741681443695
40	0.05735721643349232
41	0.0700936376052799
42	0.05966406822736201
43	0.04957023784291812
44	0.06092038188036147
45	0.014837497453528038
46	0.008187699324720582
47	0.01370031699176129
48	0.020036036707318884
49	0.10835705257120293
50	0.00836098396651361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9233363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.755959708270375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53828563650875	23.072261032483137
2	11.666971309864465	6.943238564277468
3	3.891391969768848	3.473763079845862
4	1.7913327731542992	2.1321130328833426
5	1.0441921445326994	1.5535469690203718
6	0.6852467218516517	1.2234104307385263
7	0.4582508551978496	0.9544985788483356
8	0.3625670556289582	0.8630824559073201
9	0.26049442114634674	0.6976135349873916
>10	1.7902546494123306	10.525281877396564
>50	0.22061587252884415	4.591411990475459
>100	0.22359136183726339	14.143077190726224
>500	0.03598345545251112	7.440840807348062
>1k	0.028808474396524685	16.385718631759136
>5k	0.001500822681170014	2.984870710667084
>10k+	5.124760374726876E-4	3.015271112635708
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	36200	0.3920565020567262	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30133	0.32634913194683235	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	27999	0.30323729284768725	No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG	22879	0.24778620747391822	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	21716	0.23519058007358748	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18619	0.20164917159652446	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	18399	0.19926650777187033	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	15717	0.170219669691314	No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCAGCTATGG	15214	0.16477203376494567	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15179	0.1643929736110234	No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCACAGCCAT	14938	0.16178287369401592	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTTGCAGACA	13871	0.15022695414444337	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	13500	0.14620891651286752	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	12462	0.13496707537654482	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	9565	0.10359172492189467	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	9351	0.10127404283791291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1660580224128522E-5	0.0	0.0	0.0	0.0
2	2.1660580224128522E-5	0.0	0.0	0.0	0.0
3	2.1660580224128522E-5	1.0830290112064261E-5	0.0	0.0	0.0
4	2.1660580224128522E-5	1.0830290112064261E-5	0.0	0.0	0.0
5	2.1660580224128522E-5	3.249087033619278E-5	0.0	0.0	0.0
6	2.1660580224128522E-5	3.249087033619278E-5	0.0	0.0	0.0
7	2.1660580224128522E-5	3.249087033619278E-5	0.0	0.0	0.0
8	2.1660580224128522E-5	3.249087033619278E-5	0.0	0.0	0.0
9	2.1660580224128522E-5	3.249087033619278E-5	0.0	0.0	0.0
10	3.249087033619278E-5	3.249087033619278E-5	0.0	1.0830290112064261E-5	0.0
11	4.3321160448257044E-5	3.249087033619278E-5	0.0	2.1660580224128522E-5	0.0
12	4.3321160448257044E-5	3.249087033619278E-5	0.0	3.249087033619278E-5	0.0
13	4.3321160448257044E-5	3.249087033619278E-5	0.0	3.249087033619278E-5	0.0
14	4.3321160448257044E-5	3.249087033619278E-5	0.0	3.249087033619278E-5	0.0
15	4.3321160448257044E-5	3.249087033619278E-5	0.0	4.3321160448257044E-5	0.0
16	5.41514505603213E-5	3.249087033619278E-5	0.0	6.498174067238556E-5	0.0
17	5.41514505603213E-5	3.249087033619278E-5	0.0	7.581203078444983E-5	0.0
18	6.498174067238556E-5	3.249087033619278E-5	0.0	8.664232089651409E-5	0.0
19	6.498174067238556E-5	3.249087033619278E-5	0.0	8.664232089651409E-5	0.0
20	8.664232089651409E-5	3.249087033619278E-5	0.0	1.2996348134477112E-4	0.0
21	8.664232089651409E-5	3.249087033619278E-5	0.0	1.5162406156889966E-4	0.0
22	8.664232089651409E-5	3.249087033619278E-5	0.0	2.0577551212922095E-4	0.0
23	9.747261100857835E-5	5.41514505603213E-5	0.0	3.032481231377993E-4	0.0
24	1.1913319123270687E-4	5.41514505603213E-5	0.0	4.8736305504289173E-4	0.0
25	1.1913319123270687E-4	5.41514505603213E-5	0.0	5.41514505603213E-4	0.0
26	1.7328464179302818E-4	5.41514505603213E-5	0.0	6.281568264997271E-4	0.0
27	1.7328464179302818E-4	5.41514505603213E-5	0.0	8.014414682927553E-4	0.0
28	1.8411493190509243E-4	5.41514505603213E-5	0.0	0.001418768004680418	0.0
29	2.0577551212922095E-4	5.41514505603213E-5	0.0	0.002101076281740467	0.0
30	2.0577551212922095E-4	5.41514505603213E-5	0.0	0.0033898808050761135	0.0
31	2.274360923533495E-4	5.41514505603213E-5	0.0	0.0051768786735667165	0.0
32	2.274360923533495E-4	5.41514505603213E-5	0.0	0.007288785245419247	0.0
33	2.3826638246541375E-4	5.41514505603213E-5	0.0	0.0099097154525388	0.0
34	2.49096672577478E-4	5.41514505603213E-5	0.0	0.01266060914100312	0.0
35	2.49096672577478E-4	5.41514505603213E-5	0.0	0.016202114007648136	0.0
36	2.5992696268954224E-4	5.41514505603213E-5	0.0	0.020804987305275444	0.0
37	2.707572528016065E-4	5.41514505603213E-5	0.0	0.02724900992195368	0.0
38	2.707572528016065E-4	5.41514505603213E-5	0.0	0.034711079809165955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAC	4355	0.0	19.095457	3
CTAGACA	4520	0.0	18.593483	4
TAGACAT	4655	0.0	18.337032	5
GTTCTAG	4675	0.0	18.26789	1
CTCTAGT	4365	0.0	18.242916	27
CTCTACC	8735	0.0	17.96656	1
TCTACCA	9075	0.0	17.357725	2
ACTTGCA	4935	0.0	16.495821	40
GTAGACC	7115	0.0	16.388409	4
CCTTACC	9375	0.0	16.354015	28
GGTATAG	5750	0.0	16.345505	1
AATCTAC	6525	0.0	16.318472	14
CTTACCT	9515	0.0	16.13712	29
GAATCTA	6640	0.0	16.135332	13
ATCCTTA	9610	0.0	16.114325	26
TAGACCT	7420	0.0	16.09952	5
GACATGT	3770	0.0	15.813945	7
CCTCTAG	6355	0.0	15.783821	26
TATTGGT	4050	0.0	15.753564	4
TTACCTT	9880	0.0	15.696473	30
>>END_MODULE