##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558136_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9205575
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2564009309576	33.0	32.0	34.0	25.0	34.0
2	31.509133758619097	33.0	32.0	34.0	27.0	34.0
3	31.563390445463753	33.0	32.0	34.0	27.0	34.0
4	31.4630310436882	33.0	32.0	34.0	27.0	34.0
5	31.53003185569614	33.0	32.0	34.0	27.0	34.0
6	34.87369881837908	38.0	36.0	38.0	27.0	38.0
7	34.89980452063016	38.0	36.0	38.0	27.0	38.0
8	34.924336502608476	38.0	36.0	38.0	27.0	38.0
9	35.10532975941209	38.0	36.0	38.0	28.0	38.0
10	35.048768056313705	38.0	36.0	38.0	28.0	38.0
11	35.078095936429825	38.0	36.0	38.0	28.0	38.0
12	34.98027065120864	38.0	36.0	38.0	27.0	38.0
13	35.02208476928383	38.0	36.0	38.0	27.0	38.0
14	35.07699595082328	38.0	36.0	38.0	28.0	38.0
15	35.04938854987331	38.0	36.0	38.0	27.0	38.0
16	35.02512933738522	38.0	36.0	38.0	27.0	38.0
17	34.98279998805072	38.0	36.0	38.0	27.0	38.0
18	34.990412657547196	38.0	36.0	38.0	27.0	38.0
19	34.99643172751295	38.0	36.0	38.0	27.0	38.0
20	34.94626951602697	38.0	36.0	38.0	27.0	38.0
21	34.95637719534086	38.0	36.0	38.0	27.0	38.0
22	34.99061590394951	38.0	36.0	38.0	27.0	38.0
23	34.99122488274768	38.0	36.0	38.0	27.0	38.0
24	34.98278260727874	38.0	36.0	38.0	27.0	38.0
25	34.99575626726196	38.0	36.0	38.0	27.0	38.0
26	35.002998291796004	38.0	36.0	38.0	27.0	38.0
27	35.00381942464213	38.0	36.0	38.0	27.0	38.0
28	35.028004334330014	38.0	36.0	38.0	27.0	38.0
29	34.996985413730265	38.0	36.0	38.0	27.0	38.0
30	35.003513088536025	38.0	36.0	38.0	27.0	38.0
31	35.033912058725285	38.0	36.0	38.0	27.0	38.0
32	35.048280308400074	38.0	36.0	38.0	27.0	38.0
33	35.01749515918343	38.0	36.0	38.0	27.0	38.0
34	34.99978491294677	38.0	36.0	38.0	27.0	38.0
35	34.99450832783395	38.0	36.0	38.0	27.0	38.0
36	34.96725875352708	38.0	36.0	38.0	27.0	38.0
37	34.95812570100184	38.0	36.0	38.0	27.0	38.0
38	34.93947472048188	38.0	36.0	38.0	26.0	38.0
39	34.96038031301684	38.0	36.0	38.0	27.0	38.0
40	34.93832280981905	38.0	36.0	38.0	26.0	38.0
41	34.937733927538474	38.0	36.0	38.0	25.0	38.0
42	34.83196954019711	38.0	36.0	38.0	25.0	38.0
43	34.882046803160044	38.0	36.0	38.0	25.0	38.0
44	34.86460997819256	38.0	36.0	38.0	25.0	38.0
45	34.87779144703074	38.0	36.0	38.0	25.0	38.0
46	34.85450827351904	38.0	36.0	38.0	25.0	38.0
47	34.78127167504474	38.0	36.0	38.0	25.0	38.0
48	34.733255228489256	38.0	36.0	38.0	25.0	38.0
49	34.66124679881485	38.0	36.0	38.0	25.0	38.0
50	34.26884176164987	38.0	34.0	38.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	31.0
4	637.0
5	1263.0
6	1513.0
7	662.0
8	202.0
9	149.0
10	136.0
11	118.0
12	110.0
13	90.0
14	105.0
15	200.0
16	508.0
17	2024.0
18	10889.0
19	34745.0
20	62827.0
21	82936.0
22	94184.0
23	103272.0
24	113438.0
25	125949.0
26	139082.0
27	156626.0
28	176208.0
29	202233.0
30	233680.0
31	270093.0
32	319517.0
33	392916.0
34	513758.0
35	751760.0
36	1471250.0
37	3942464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15506026605135	18.116309327620662	13.485167469938702	24.24346293638929
2	14.674466288091729	21.634878864166552	37.76359434364502	25.927060504096705
3	18.614817651260243	27.408282480996572	29.339307973700716	24.63759189404247
4	12.912367793453743	17.815362094023275	35.85079636554971	33.42147374697328
5	13.87720202666035	37.19725715303871	33.920801190312574	15.004739629988359
6	31.365772657199294	34.92846645287888	18.752764239563334	14.952996650358491
7	26.32866895329442	32.03810055908429	23.311146413561502	18.322084074059784
8	27.899352096021495	31.54406335138423	21.374787390031717	19.181797162562557
9	27.583877546672785	14.801428975757236	19.819962880020032	37.79473059754994
10	17.00550236844574	27.829544519464278	31.20199124016943	23.96296187192055
11	33.03470748326235	23.621819237954078	21.931108516401995	21.41236476238158
12	23.621765925229646	25.936379405788813	29.446608588924782	20.995246080056766
13	29.55099458615118	20.54898307581182	25.39391307514331	24.506109262893695
14	24.154929278925813	20.82078903811665	25.86820726210206	29.156074420855475
15	26.465065668545503	25.329810083868654	25.0071438516844	23.197980395901446
16	23.549579500909847	26.285391549322107	26.95368134939713	23.211347600370917
17	23.564077368076646	26.2483722224517	27.298487118002186	22.889063291469466
18	24.86175694835353	23.789806639101766	29.46685460720666	21.88158180533804
19	23.784279302075237	26.97325001434735	28.482634921628673	20.759835761948736
20	24.694700961722404	23.655566859706788	29.392369263143248	22.25736291542756
21	24.505627695087867	25.664926470356463	26.919472108690336	22.90997372586533
22	23.923834878771356	25.443989876501533	26.185102389729476	24.447072854997632
23	21.83986211038538	26.118904903965152	27.10374532808071	24.937487657568763
24	22.673177147018382	28.09462477786654	26.43337626554934	22.798821809565734
25	24.07744438949365	24.119465443498203	25.942848757900688	25.86024140910746
26	23.63434725540248	24.890155997123355	27.397382654912732	24.07811409256143
27	24.642680914795985	24.842556142223344	26.052187106689246	24.46257583629143
28	23.33206106538385	24.412351316362614	28.234621714744772	24.020965903508763
29	23.503172154604275	24.22486341387487	29.200876603359806	23.071087828161048
30	23.78001243586027	25.444724383535323	28.68008048420949	22.095182696394915
31	25.282112380643092	24.139446650420194	26.381129571168316	24.197311397768402
32	21.864989684967092	25.259723421047887	28.187982046560784	24.68730484742424
33	23.007428430195514	25.67799397386996	27.483757034053657	23.83082056188087
34	21.895854197267404	25.355747511598842	28.176348510285216	24.572049780848534
35	23.106609125596375	26.191697412662624	28.465145752052766	22.23654770968824
36	24.005249429409847	25.53159439191392	27.094641886751443	23.36851429192479
37	24.299407837311175	25.850369119416268	27.44202304155529	22.408200001717265
38	24.483876781308542	25.082093836869735	26.68017632484398	23.753853056977743
39	24.559990304553327	24.022208224934364	27.00498195962151	24.412819510890802
40	24.184853669612195	25.806125197242185	26.476036718403044	23.532984414742575
41	22.148081727476303	26.281708105689646	26.951146589363923	24.619063577470126
42	25.873352727343867	26.214499640506126	26.730725232013487	21.181422400136515
43	25.18602540489672	23.61459476854187	26.131935530393164	25.067444296168244
44	23.769356233830628	25.311365997461376	27.536508183567737	23.382769585140256
45	26.260359401142956	24.089893744160275	26.577334260446317	23.07241259425045
46	22.59895187327746	24.92267352163865	29.580404925558973	22.89796967952492
47	24.979066846915774	24.75367216992842	27.760462801132384	22.50679818202342
48	24.89126881140016	26.3314565801587	26.637573895866	22.139700712575134
49	23.43432623317569	25.567657565637553	29.18348241456904	21.814533786617716
50	24.05892848044396	26.35502163912884	25.711882634084432	23.874167246342772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	492.0
1	812.0
2	1132.0
3	3766.0
4	6400.0
5	7822.0
6	9244.0
7	8598.0
8	7952.0
9	10130.0
10	12308.0
11	16974.0
12	21640.0
13	37250.5
14	52861.0
15	64746.5
16	76632.0
17	71026.0
18	65420.0
19	57113.5
20	48807.0
21	53543.0
22	58279.0
23	66658.5
24	75038.0
25	86336.5
26	97635.0
27	108153.0
28	118671.0
29	136215.0
30	153759.0
31	179536.5
32	205314.0
33	224484.5
34	243655.0
35	266820.0
36	289985.0
37	317861.0
38	345737.0
39	371120.0
40	396503.0
41	419544.5
42	442586.0
43	451717.0
44	460848.0
45	541090.0
46	621332.0
47	793079.5
48	964827.0
49	976407.0
50	987987.0
51	828955.0
52	669923.0
53	557996.5
54	446070.0
55	462761.0
56	479452.0
57	458609.5
58	437767.0
59	411576.5
60	385386.0
61	332094.0
62	278802.0
63	245117.5
64	211433.0
65	179354.5
66	147276.0
67	133165.5
68	119055.0
69	101712.0
70	84369.0
71	68085.5
72	51802.0
73	49960.5
74	48119.0
75	37019.0
76	25919.0
77	21841.0
78	17763.0
79	14555.0
80	11347.0
81	10139.0
82	8931.0
83	8500.0
84	8069.0
85	6365.5
86	4662.0
87	4407.5
88	4153.0
89	2145.0
90	137.0
91	106.5
92	76.0
93	44.5
94	13.0
95	9.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04391903819153068
2	0.0
3	0.0
4	0.014360862846698874
5	0.01379598775741874
6	0.05959432191905449
7	0.06202762999595354
8	0.06754602509892103
9	0.05582486699635818
10	0.06606865948080375
11	0.06145189192418725
12	0.05842111981054958
13	0.06539515456666205
14	0.0634615436841262
15	0.05818213419585414
16	0.06163656262645191
17	0.06425454140561562
18	0.07518270178668904
19	0.05725878068453084
20	0.058823050162537374
21	0.057269643667017
22	0.0478840267989778
23	0.04988281557643059
24	0.05458648699293634
25	0.05893167998739894
26	0.049665555926707454
27	0.05252252032056661
28	0.04517914415992483
29	0.059952800341097644
30	0.05175124856404951
31	0.052240082775926545
32	0.048242505221020957
33	0.05406506383360084
34	0.055281717872050364
35	0.05637887910315216
36	0.04969814487416593
37	0.05316343628724984
38	0.06232093052307977
39	0.0584428457755219
40	0.053847804183877704
41	0.0659817556209145
42	0.05583572997884434
43	0.04647183907577745
44	0.057671574019004794
45	0.01493660091846517
46	0.008386222479312807
47	0.013861165652335677
48	0.020791748478503513
49	0.10408909818235144
50	0.007843073355004984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9205575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.12053259364059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50730796600793	26.551231132569665
2	13.789485500834111	9.961671209648259
3	5.159504603516073	5.590921625950229
4	2.347014328161213	3.391016301523543
5	1.336748931518802	2.414204167521956
6	0.8175201381748858	1.7717557678142113
7	0.5529054246959778	1.3979866889752233
8	0.3822697432884804	1.1046229377611345
9	0.2894516268809729	0.940963223073283
>10	1.5463882717451798	10.288568420276158
>50	0.13377546382546598	3.3658246249794623
>100	0.10899112595238773	8.081245727412265
>500	0.014077117269947632	3.536895519309751
>1k	0.01256609830798195	9.052329886737128
>5k	0.0011478647452537666	2.8940880953147445
>10k+	8.45795075450144E-4	9.656674671133052
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	108227	1.175668005529258	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	86482	0.9394524513677853	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	70542	0.7662965105384509	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	61916	0.672592423612865	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	50154	0.5448220236106924	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41868	0.4548113507303998	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	41591	0.4518023045817344	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	39158	0.42537266819291575	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	32147	0.3492122979824726	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	27740	0.3013391341659809	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	27217	0.2956577943257211	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	26273	0.2854031388587894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25829	0.2805799746349359	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	24501	0.26615393389332004	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	23387	0.2540525714037417	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21644	0.235118392930371	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	18830	0.20454996021432664	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	18508	0.20105207985378426	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	18251	0.19826029335484205	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	18152	0.19718485808871253	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	15269	0.1658668795811234	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	14261	0.15491699323507765	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	14012	0.15221211059602469	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	13165	0.14301116443025014	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	12408	0.1347878866882297	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	11682	0.12690136140328007	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	11013	0.11963402612004138	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	10820	0.11753747050021318	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	9860	0.10710900731350295	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	9844	0.10693519959372447	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	9792	0.10637032450444431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.517789491693892E-5	0.0	0.0	0.0	0.0
2	6.517789491693892E-5	0.0	0.0	0.0	0.0
3	6.517789491693892E-5	0.0	0.0	0.0	0.0
4	6.517789491693892E-5	0.0	0.0	1.0862982486156486E-5	0.0
5	7.60408774030954E-5	0.0	0.0	2.1725964972312972E-5	0.0
6	7.60408774030954E-5	0.0	0.0	2.1725964972312972E-5	0.0
7	7.60408774030954E-5	0.0	0.0	2.1725964972312972E-5	0.0
8	1.0862982486156487E-4	0.0	0.0	2.1725964972312972E-5	0.0
9	1.0862982486156487E-4	0.0	0.0	2.1725964972312972E-5	0.0
10	1.1949280734772135E-4	0.0	0.0	7.60408774030954E-5	0.0
11	1.8467070226466027E-4	0.0	0.0	1.1949280734772135E-4	0.0
12	1.8467070226466027E-4	0.0	0.0	1.520817548061908E-4	0.0
13	1.8467070226466027E-4	1.0862982486156486E-5	0.0	1.629447372923473E-4	1.0862982486156486E-5
14	1.8467070226466027E-4	1.0862982486156486E-5	0.0	1.8467070226466027E-4	1.0862982486156486E-5
15	1.8467070226466027E-4	1.0862982486156486E-5	0.0	2.498485971815992E-4	1.0862982486156486E-5
16	2.2812263220928623E-4	1.0862982486156486E-5	0.0	3.041635096123816E-4	1.0862982486156486E-5
17	2.2812263220928623E-4	1.0862982486156486E-5	0.0	3.3675245707085106E-4	1.0862982486156486E-5
18	2.2812263220928623E-4	1.0862982486156486E-5	0.0	3.6934140452932055E-4	1.0862982486156486E-5
19	2.389856146954427E-4	1.0862982486156486E-5	0.0	4.0193035198779E-4	1.0862982486156486E-5
20	2.498485971815992E-4	1.0862982486156486E-5	0.0	4.671082469047289E-4	1.0862982486156486E-5
21	3.150264920985381E-4	1.0862982486156486E-5	0.0	5.757380717662938E-4	1.0862982486156486E-5
22	3.150264920985381E-4	1.0862982486156486E-5	0.0	6.952308791140151E-4	1.0862982486156486E-5
23	3.4761543955700756E-4	2.1725964972312972E-5	0.0	0.0010537093011571792	1.0862982486156486E-5
24	3.4761543955700756E-4	2.1725964972312972E-5	0.0	0.0014230507056864998	1.0862982486156486E-5
25	3.5847842204316405E-4	2.1725964972312972E-5	0.0	0.0015642694780065342	1.0862982486156486E-5
26	4.671082469047289E-4	2.1725964972312972E-5	0.0	0.0019444738650220112	1.0862982486156486E-5
27	4.779712293908854E-4	2.1725964972312972E-5	0.0	0.0024658970243575224	1.0862982486156486E-5
28	4.779712293908854E-4	2.1725964972312972E-5	0.0	0.004334330011976438	1.0862982486156486E-5
29	4.888342118770419E-4	2.1725964972312972E-5	0.0	0.006257077912026137	1.0862982486156486E-5
30	4.888342118770419E-4	2.1725964972312972E-5	0.0	0.009320438973122265	1.0862982486156486E-5
31	5.322861418216679E-4	2.1725964972312972E-5	0.0	0.01344837231786173	1.0862982486156486E-5
32	5.322861418216679E-4	2.1725964972312972E-5	0.0	0.017511127767684258	1.0862982486156486E-5
33	5.322861418216679E-4	3.258894745846946E-5	0.0	0.022497236728830083	1.0862982486156486E-5
34	5.431491243078244E-4	3.258894745846946E-5	0.0	0.02766801639224057	1.0862982486156486E-5
35	5.431491243078244E-4	3.258894745846946E-5	0.0	0.035206926237633177	1.0862982486156486E-5
36	5.540121067939808E-4	3.258894745846946E-5	0.0	0.04454909117572775	1.0862982486156486E-5
37	6.083270192247633E-4	3.258894745846946E-5	0.0	0.05747604033425397	1.0862982486156486E-5
38	6.191900017109197E-4	3.258894745846946E-5	0.0	0.0730209682719439	1.0862982486156486E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTCG	670	0.0	14.776165	15
GTCGACG	715	0.0	14.768153	36
CCGTCGT	2505	0.0	14.753736	9
GTATTAG	1485	0.0	14.672295	1
GTACTAG	1065	0.0	14.672295	1
CGACGAA	775	0.0	14.476284	38
CGTCGTA	2530	0.0	14.434042	10
TACCGTC	2735	0.0	14.318143	7
AGTCGAC	740	0.0	14.269539	35
ATTCGTA	3645	0.0	13.820364	24
TAGGTCG	590	0.0	13.795379	21
GACGGCC	3740	0.0	13.76385	11
GCATTCG	3635	0.0	13.676313	22
CGCTAGA	3680	0.0	13.629439	37
GGCGTTA	2330	0.0	13.597052	42
TCGACGA	810	0.0	13.579256	37
ATACCGT	3015	0.0	13.426245	6
GTCGTAG	2695	0.0	13.305296	11
CGTATTG	3795	0.0	13.273961	27
CGCAATA	1520	0.0	13.170091	36
>>END_MODULE