##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10077264
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.218403328522506	33.0	33.0	34.0	30.0	34.0
2	32.24891418940697	33.0	33.0	34.0	30.0	34.0
3	32.250042968011954	33.0	33.0	34.0	30.0	34.0
4	32.216154900774654	33.0	33.0	34.0	30.0	34.0
5	32.23742277665843	33.0	33.0	34.0	31.0	34.0
6	35.81569173934513	38.0	37.0	38.0	31.0	38.0
7	35.92590330073718	38.0	37.0	38.0	31.0	38.0
8	35.926452656197156	38.0	37.0	38.0	31.0	38.0
9	36.062305998929865	38.0	38.0	38.0	31.0	38.0
10	35.996170091405766	38.0	37.0	38.0	31.0	38.0
11	36.075048346455944	38.0	38.0	38.0	31.0	38.0
12	36.02503427517628	38.0	38.0	38.0	31.0	38.0
13	36.06846560733151	38.0	38.0	38.0	31.0	38.0
14	36.085355310727195	38.0	38.0	38.0	32.0	38.0
15	36.049091003272316	38.0	38.0	38.0	31.0	38.0
16	36.039467855560794	38.0	38.0	38.0	31.0	38.0
17	35.98576091685204	38.0	38.0	38.0	31.0	38.0
18	35.999177951475716	38.0	38.0	38.0	31.0	38.0
19	35.92983809891256	38.0	38.0	38.0	31.0	38.0
20	35.919800354540676	38.0	37.0	38.0	31.0	38.0
21	35.93555919543241	38.0	37.0	38.0	31.0	38.0
22	35.971696186583976	38.0	38.0	38.0	31.0	38.0
23	35.963279715605346	38.0	38.0	38.0	31.0	38.0
24	35.87752657864277	38.0	38.0	38.0	31.0	38.0
25	35.93703687826378	38.0	38.0	38.0	31.0	38.0
26	35.89060612086773	38.0	37.0	38.0	31.0	38.0
27	35.89216467882552	38.0	37.0	38.0	31.0	38.0
28	35.86761734137361	38.0	37.0	38.0	31.0	38.0
29	35.90553378377306	38.0	37.0	38.0	31.0	38.0
30	35.87627911703018	38.0	37.0	38.0	31.0	38.0
31	35.941961627679895	38.0	37.0	38.0	31.0	38.0
32	35.95208858277405	38.0	37.0	38.0	31.0	38.0
33	35.91679854770104	38.0	37.0	38.0	31.0	38.0
34	35.91786153463877	38.0	37.0	38.0	31.0	38.0
35	35.845497051580665	38.0	37.0	38.0	31.0	38.0
36	35.864893288495765	38.0	37.0	38.0	31.0	38.0
37	35.81499532015833	38.0	37.0	38.0	30.0	38.0
38	35.79714702323964	38.0	37.0	38.0	29.0	38.0
39	35.78663802000226	38.0	37.0	38.0	29.0	38.0
40	35.77370276297217	38.0	37.0	38.0	29.0	38.0
41	35.76298298823966	38.0	37.0	38.0	29.0	38.0
42	35.7249887469456	38.0	37.0	38.0	29.0	38.0
43	35.77685808370209	38.0	37.0	38.0	29.0	38.0
44	35.72557769648587	38.0	37.0	38.0	29.0	38.0
45	35.76244196837554	38.0	37.0	38.0	29.0	38.0
46	35.727063020280106	38.0	37.0	38.0	29.0	38.0
47	35.62849033229654	38.0	37.0	38.0	29.0	38.0
48	35.640849937046404	38.0	37.0	38.0	29.0	38.0
49	35.529419691694095	38.0	37.0	38.0	29.0	38.0
50	35.225197136841906	38.0	36.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	190.0
5	560.0
6	1282.0
7	605.0
8	308.0
9	248.0
10	207.0
11	169.0
12	154.0
13	150.0
14	182.0
15	178.0
16	327.0
17	1290.0
18	6553.0
19	20123.0
20	35315.0
21	47374.0
22	57498.0
23	67303.0
24	77560.0
25	89436.0
26	103339.0
27	117983.0
28	136650.0
29	159657.0
30	186240.0
31	217743.0
32	261354.0
33	326412.0
34	432974.0
35	661897.0
36	1439914.0
37	5626082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26304093988691	18.50378541408764	13.531193918481549	24.70197972754389
2	14.953572715768884	21.225602504806858	37.925948948047804	25.894875831376453
3	18.176584963691425	26.938771095322387	29.183206423508672	25.701437517477515
4	12.799292409718827	17.591851081341723	35.99708763048751	33.61176887845193
5	14.115555987926912	36.99164503017528	33.70092950466291	15.191869477234905
6	31.579054475279843	35.56572850114557	18.211396963933307	14.64382005964128
7	26.91576643069254	31.721792280673043	23.02995826183179	18.332483026802628
8	27.669321710380977	32.068754793599105	20.877450165185255	19.384473330834666
9	27.431315427294006	14.757401119016592	19.77867879632941	38.03260465736
10	17.190042337056347	27.62875448588214	30.956471540619457	24.22473163644206
11	33.539074670344235	23.486899652199643	22.135105000362394	20.83892067709373
12	23.79374723363848	25.778855496294938	29.002290929590803	21.425106340475782
13	29.46240553583533	20.807113418150383	25.021036158366634	24.709444887647656
14	24.01995875579621	20.721435043357243	25.755449648288575	29.503156552557968
15	25.957878544700204	26.06625729980314	24.12384985962891	23.852014295867747
16	23.79350891175451	26.34077399212371	26.193177959892637	23.672539136229144
17	23.59325046538638	26.444906700839176	26.640363521093857	23.32147931268058
18	24.778167643338307	24.484952690755772	28.428864074769468	22.308015591136453
19	24.12364974107833	26.63259255670204	27.62552607425982	21.61823162795981
20	24.723317171700323	24.210916558594807	28.144950725784206	22.92081554392066
21	24.95116499664208	25.483617180430656	26.475456421268106	23.089761401659153
22	24.364895088011068	25.178529874045253	25.98118462111519	24.47539041682849
23	22.50530201608175	25.817837035601325	26.854588578545354	24.82227236977157
24	23.080884877861564	27.45891317277201	26.105866514631664	23.354335434734764
25	24.18767007671441	24.310482230808812	25.948143089165498	25.553704603311285
26	23.822538336116978	25.243254109080805	26.89295171349228	24.04125584130994
27	24.490149260902943	25.057652221073596	25.795840899427265	24.656357618596196
28	23.566376862928305	24.729089527574704	27.53073634398577	24.173797265511222
29	23.839732861629255	24.45208380199627	28.184910883390835	23.523272452983633
30	24.00315726388597	25.364064670501037	27.877089476028612	22.755688589584384
31	25.283168308181793	24.271166491012238	26.229289786919395	24.216375413886574
32	22.58348100159433	25.2844129917363	27.394645466629886	24.737460540039482
33	23.325615915187086	25.504993225461543	27.09282313863488	24.076567720716486
34	22.68253972350838	25.18422086610163	27.460181192129134	24.673058218260852
35	23.44698727314928	25.935959314740725	27.819860431325854	22.797192980784143
36	24.225650945015538	25.408127799746826	26.586663428971423	23.779557826266213
37	24.362782469891716	25.666045242831785	26.96133671642028	23.009835570856215
38	24.43655139630572	25.227581490713863	26.43450899067514	23.90135812230527
39	24.485055817058406	24.371698203870558	26.504316484766317	24.63892949430472
40	24.2861617316313	25.784179461126204	26.333695521066367	23.59596328617613
41	22.811265234156277	25.997958851397236	26.630158963382428	24.560616951064056
42	25.565930637576944	26.18310103154602	26.336584515938803	21.914383814938233
43	25.072140352075493	23.930175406339853	26.058784881073905	24.93889936051075
44	23.869347207588973	25.435055460853768	26.93011047894594	23.765486852611314
45	25.568874646927974	24.41300535542187	26.431936287468506	23.58618371018165
46	23.114259981677566	24.940172253103622	28.478761695634848	23.466806069583967
47	24.8755912319058	24.99061253133787	27.122520557167107	23.01127567958922
48	24.92090114935959	26.095972081310958	26.223655547775664	22.759471221553788
49	23.79464856972988	25.673227272831056	27.90287241320055	22.62925174423851
50	24.213508234985888	26.20043427691306	25.563359175588396	24.022698312512652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1016.0
1	1210.5
2	1405.0
3	3917.0
4	6429.0
5	7291.0
6	8153.0
7	7261.0
8	6369.0
9	8242.5
10	10116.0
11	14492.5
12	18869.0
13	34510.0
14	50151.0
15	62011.0
16	73871.0
17	69957.5
18	66044.0
19	59073.0
20	52102.0
21	56744.5
22	61387.0
23	71322.5
24	81258.0
25	95760.0
26	110262.0
27	124339.0
28	138416.0
29	160625.5
30	182835.0
31	204810.0
32	226785.0
33	248272.5
34	269760.0
35	298544.0
36	327328.0
37	359008.5
38	390689.0
39	417770.5
40	444852.0
41	466134.0
42	487416.0
43	500028.5
44	512641.0
45	578833.0
46	645025.0
47	786564.5
48	928104.0
49	948634.0
50	969164.0
51	827155.5
52	685147.0
53	596414.0
54	507681.0
55	527401.0
56	547121.0
57	533095.0
58	519069.0
59	488634.0
60	458199.0
61	404713.5
62	351228.0
63	309835.5
64	268443.0
65	231344.5
66	194246.0
67	174183.0
68	154120.0
69	131190.5
70	108261.0
71	86135.5
72	64010.0
73	60395.0
74	56780.0
75	44274.5
76	31769.0
77	26119.0
78	20469.0
79	16697.0
80	12925.0
81	11314.5
82	9704.0
83	9098.0
84	8492.0
85	6605.0
86	4718.0
87	4436.5
88	4155.0
89	2170.0
90	185.0
91	136.0
92	87.0
93	44.5
94	2.0
95	2.5
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03176457419394788
2	0.0
3	2.9769985186455373E-4
4	0.0024411387852893403
5	0.03591252546326066
6	0.0367560083768769
7	0.063370375133568
8	0.05198831746394656
9	0.05166084762689556
10	0.05899418731115906
11	0.053526433365246755
12	0.053288273483755116
13	0.04100319293014453
14	0.040596336465929646
15	0.06055214986925023
16	0.04465497777968305
17	0.04416873468830429
18	0.050916597997234164
19	0.0544989195480043
20	0.05221655401704271
21	0.040278789957274116
22	0.04405957807595395
23	0.06048268657048183
24	0.04113219619928584
25	0.04932886545395655
26	0.035118659191621854
27	0.043315328446292566
28	0.03979254686589535
29	0.0424916921894673
30	0.039544463656008215
31	0.04421835133028171
32	0.03418586632244625
33	0.034880499310130215
34	0.0372918681102331
35	0.04197567911290207
36	0.0422237623227892
37	0.035962142105238085
38	0.047909829493402174
39	0.04679841671310784
40	0.04186652250055174
41	0.044694671093265
42	0.04484352101919727
43	0.04503206425871149
44	0.04091388297458516
45	0.0
46	0.0
47	0.0
48	0.0
49	0.03926661046093464
50	0.007382956326240931
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0077264E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.935293294820404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.90612750443852	24.42191067472671
2	15.349077988713136	10.724490826815309
3	5.842740714380503	6.123535815074141
4	2.7368062161488735	3.824445114089942
5	1.5401158587954002	2.690219961751076
6	0.9566127219186489	2.0051727605870684
7	0.6578478748764309	1.60874759165277
8	0.4689700112646402	1.3106883912004348
9	0.3368932391816737	1.0592517707868465
>10	1.8725831185534711	12.090820825451148
>50	0.17596298457527557	4.320053284876225
>100	0.12917196848996534	8.983045454316272
>500	0.013847037708823937	3.3128547504736074
>1k	0.011551585915396524	8.214298608890882
>5k	9.74575446333462E-4	2.3221509854461724
>10k+	7.165995928922515E-4	6.988313183861562
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	89635	0.8894775407293091	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	70036	0.6949902275061961	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	55538	0.5511218124284528	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	46749	0.46390567916053405	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	40656	0.40344283924684315	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40551	0.40240088976531724	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	33853	0.3359344361723579	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	32987	0.3273408337818678	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25381	0.25186399800580794	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	24130	0.23944991418305603	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	22951	0.2277503100047791	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	21965	0.21796590820683076	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	21820	0.2165270255894854	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21457	0.2129248573819243	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	18602	0.18459375481281426	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18214	0.18074350339536605	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	15021	0.14905831582858203	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	14557	0.14445389145307694	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	14417	0.14306462547770904	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	13728	0.13622745221321977	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	11720	0.11630140879508565	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	11480	0.11391980998016922	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	11136	0.11050618501212235	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	10790	0.1070727133872845	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	10491	0.10410563819703443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9846656790970246E-5	0.0	0.0	0.0	0.0
2	1.9846656790970246E-5	0.0	0.0	0.0	0.0
3	1.9846656790970246E-5	0.0	0.0	0.0	1.9846656790970246E-5
4	1.9846656790970246E-5	0.0	0.0	9.923328395485123E-6	1.9846656790970246E-5
5	1.9846656790970246E-5	0.0	0.0	9.923328395485123E-6	1.9846656790970246E-5
6	2.976998518645537E-5	0.0	0.0	9.923328395485123E-6	2.976998518645537E-5
7	2.976998518645537E-5	0.0	0.0	9.923328395485123E-6	2.976998518645537E-5
8	3.969331358194049E-5	0.0	0.0	9.923328395485123E-6	4.961664197742562E-5
9	3.969331358194049E-5	0.0	0.0	1.9846656790970246E-5	4.961664197742562E-5
10	5.953997037291074E-5	0.0	0.0	6.946329876839587E-5	6.946329876839587E-5
11	8.930995555936611E-5	0.0	0.0	6.946329876839587E-5	6.946329876839587E-5
12	9.923328395485124E-5	0.0	0.0	8.930995555936611E-5	6.946329876839587E-5
13	9.923328395485124E-5	0.0	0.0	8.930995555936611E-5	6.946329876839587E-5
14	9.923328395485124E-5	0.0	0.0	9.923328395485124E-5	6.946329876839587E-5
15	9.923328395485124E-5	0.0	0.0	9.923328395485124E-5	1.0915661235033635E-4
16	1.3892659753679174E-4	0.0	0.0	1.290032691413066E-4	1.4884992593227687E-4
17	1.4884992593227687E-4	0.0	0.0	1.686965827232471E-4	1.4884992593227687E-4
18	1.5877325432776197E-4	9.923328395485123E-6	0.0	1.8854323951421735E-4	1.686965827232471E-4
19	1.5877325432776197E-4	9.923328395485123E-6	0.0	2.480832098871281E-4	1.686965827232471E-4
20	1.686965827232471E-4	9.923328395485123E-6	0.0	2.778531950735835E-4	1.7861991111873222E-4
21	1.9846656790970248E-4	9.923328395485123E-6	0.0	3.1754650865552394E-4	2.183132247006727E-4
22	1.9846656790970248E-4	9.923328395485123E-6	0.0	5.060897481697413E-4	2.183132247006727E-4
23	2.083898963051876E-4	1.9846656790970246E-5	0.0	6.251696889155628E-4	2.183132247006727E-4
24	2.2823655309615784E-4	1.9846656790970246E-5	0.0	8.83176227198176E-4	2.183132247006727E-4
25	2.2823655309615784E-4	1.9846656790970246E-5	0.0	0.0010221028247349678	2.2823655309615784E-4
26	3.373931654464942E-4	1.9846656790970246E-5	0.0	0.001190799407458215	2.3815988149164297E-4
27	3.5723982223746445E-4	1.9846656790970246E-5	0.0	0.0015480392296956793	2.480832098871281E-4
28	4.167797926103752E-4	1.9846656790970246E-5	0.0	0.0025899887112216173	2.480832098871281E-4
29	4.366264494013454E-4	1.9846656790970246E-5	0.0	0.0038006347754708024	2.480832098871281E-4
30	4.4654977779683055E-4	1.9846656790970246E-5	0.0	0.005715837155799431	2.480832098871281E-4
31	4.564731061923157E-4	1.9846656790970246E-5	0.0	0.008960765541123067	2.480832098871281E-4
32	4.564731061923157E-4	1.9846656790970246E-5	0.0	0.012096537314096366	2.480832098871281E-4
33	4.564731061923157E-4	1.9846656790970246E-5	0.0	0.015926942074753623	2.480832098871281E-4
34	4.564731061923157E-4	1.9846656790970246E-5	0.0	0.020660369719400027	2.480832098871281E-4
35	4.663964345878008E-4	1.9846656790970246E-5	0.0	0.027031146549301478	2.480832098871281E-4
36	4.961664197742562E-4	1.9846656790970246E-5	0.0	0.034701879399011475	2.480832098871281E-4
37	5.259364049607115E-4	1.9846656790970246E-5	0.0	0.04581600720195481	2.480832098871281E-4
38	5.457830617516818E-4	1.9846656790970246E-5	0.0	0.05867664080250354	2.9769985186455373E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTTA	2450	0.0	16.51931	42
GTACTAG	1100	0.0	16.406088	1
AATGCGA	775	0.0	16.180704	20
TCGACGA	640	0.0	15.810595	37
CGTCGTA	2535	0.0	15.797282	10
CGACGAA	630	0.0	15.712392	38
CCGTCGT	2625	0.0	15.33956	9
GTCGACG	625	0.0	15.134176	36
TACCGTC	2740	0.0	15.016665	7
CGGCGTT	2730	0.0	14.825242	41
TGCGACG	840	0.0	14.666356	22
ATACCGT	2985	0.0	14.5949745	6
GCGTTAT	2875	0.0	14.3887825	43
GTCGTAG	2825	0.0	14.174769	11
TAGGTCG	685	0.0	14.130946	21
CGTTATT	2935	0.0	13.943721	44
ACCGTCG	2975	0.0	13.903883	8
GTCCTAT	2535	0.0	13.543487	1
CGAACAA	750	0.0	13.490972	41
GCGGCGT	2995	0.0	13.440316	40
>>END_MODULE