FastQCFastQC Report
Wed 25 May 2016
SRR2558131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9299648
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256410.27572011327740575No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA246320.26487024024995354No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTT220340.23693369899591898No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT198030.21294354366960982No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTA174560.18770602930347471No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGCAGAAATG167300.17989928220939116No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTCATCTCAT166510.17904978769088895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156180.1679418403793348No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG147110.1581887830593158No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATC142150.15285524785454246No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTC141340.15198424714569841No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC135070.14524205647353533No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTATAAAGTCC128490.13816651985107392No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCTGGATTAT126200.13570406105693464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125100.1345212205881341No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA120320.1293812410964372No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGCAATGAAT120130.12917693228818986No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG105520.1134666602434845No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGAAAATTTA100430.10799333480148926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG10600.019.09217524
AATGCGA12100.017.81836720
GTACTAG14800.017.5473331
TGCGACG11500.017.40859422
CATTGCG12850.017.117329
CAATGCG12350.017.10249519
TCGACGA11500.016.45004537
CGTCATT13550.016.07189226
AGGTCGC28950.016.03807610
AGGTCGT14500.015.930943522
CGACGAA11600.015.92897138
GTCGACG11450.015.75341936
TAGGTCG14450.015.52932421
ATTAGGT15500.015.04628919
GCTCGTC14800.014.8625439
AGTCGAC12450.014.84153135
CGCGAAT6250.014.78216535
AAGTCGA12750.014.6645334
GTCGCCA31900.014.62162812
GGTCGCC31550.014.50629811
Produced by FastQC (version 0.11.5)