##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11908639
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27912593538187	33.0	33.0	34.0	31.0	34.0
2	32.29198659897239	33.0	33.0	34.0	30.0	34.0
3	32.30732983005027	33.0	33.0	34.0	30.0	34.0
4	32.271210589220146	33.0	33.0	34.0	31.0	34.0
5	32.290762193731794	33.0	33.0	34.0	31.0	34.0
6	35.86321098489928	38.0	37.0	38.0	31.0	38.0
7	35.967536928443295	38.0	37.0	38.0	31.0	38.0
8	35.98254351315881	38.0	37.0	38.0	31.0	38.0
9	36.09782788780481	38.0	38.0	38.0	31.0	38.0
10	36.01035575937771	38.0	38.0	38.0	31.0	38.0
11	36.10907913154475	38.0	38.0	38.0	32.0	38.0
12	36.05261533244899	38.0	38.0	38.0	31.0	38.0
13	36.096123242966726	38.0	38.0	38.0	32.0	38.0
14	36.121153979056714	38.0	38.0	38.0	33.0	38.0
15	36.08675265074372	38.0	38.0	38.0	32.0	38.0
16	36.070865612770696	38.0	38.0	38.0	32.0	38.0
17	36.01109564241556	38.0	38.0	38.0	31.0	38.0
18	36.0220936246367	38.0	38.0	38.0	31.0	38.0
19	35.96185088825012	38.0	38.0	38.0	31.0	38.0
20	35.936263749367164	38.0	37.0	38.0	31.0	38.0
21	35.9511940029419	38.0	37.0	38.0	31.0	38.0
22	36.004939271397845	38.0	38.0	38.0	31.0	38.0
23	35.99673942589073	38.0	38.0	38.0	31.0	38.0
24	35.922789749525535	38.0	38.0	38.0	31.0	38.0
25	35.984141176838094	38.0	38.0	38.0	31.0	38.0
26	35.9226517824581	38.0	37.0	38.0	31.0	38.0
27	35.92443359816348	38.0	37.0	38.0	31.0	38.0
28	35.89484524637954	38.0	37.0	38.0	31.0	38.0
29	35.92497421409785	38.0	37.0	38.0	31.0	38.0
30	35.89121258944872	38.0	37.0	38.0	31.0	38.0
31	35.965881323634044	38.0	37.0	38.0	31.0	38.0
32	35.96396842661869	38.0	37.0	38.0	31.0	38.0
33	35.92831246291033	38.0	37.0	38.0	31.0	38.0
34	35.911835349110845	38.0	37.0	38.0	31.0	38.0
35	35.83689353586082	38.0	37.0	38.0	31.0	38.0
36	35.863719187389925	38.0	37.0	38.0	31.0	38.0
37	35.80355227830821	38.0	37.0	38.0	29.0	38.0
38	35.7865956806651	38.0	37.0	38.0	29.0	38.0
39	35.76982894518844	38.0	37.0	38.0	29.0	38.0
40	35.74311917591926	38.0	37.0	38.0	29.0	38.0
41	35.722297149153654	38.0	37.0	38.0	29.0	38.0
42	35.67327022004782	38.0	37.0	38.0	29.0	38.0
43	35.72165274302127	38.0	37.0	38.0	29.0	38.0
44	35.653346532714615	38.0	37.0	38.0	29.0	38.0
45	35.67137756044163	38.0	37.0	38.0	29.0	38.0
46	35.599462793355315	38.0	37.0	38.0	29.0	38.0
47	35.48232237117944	38.0	37.0	38.0	28.0	38.0
48	35.47947737772553	38.0	37.0	38.0	28.0	38.0
49	35.343890263194645	38.0	37.0	38.0	27.0	38.0
50	35.056743847890594	38.0	36.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	231.0
5	721.0
6	1544.0
7	687.0
8	370.0
9	297.0
10	246.0
11	187.0
12	203.0
13	197.0
14	159.0
15	198.0
16	450.0
17	1716.0
18	8650.0
19	25436.0
20	44918.0
21	59433.0
22	69551.0
23	79271.0
24	91136.0
25	103339.0
26	117417.0
27	135020.0
28	156393.0
29	183753.0
30	215850.0
31	252387.0
32	303722.0
33	379524.0
34	512287.0
35	802570.0
36	1780469.0
37	6580300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34759227211338	18.14849118317535	13.628273580371195	23.87564296434008
2	14.584504576887417	21.52303046553011	38.42772461235914	25.46474034522333
3	18.293181541643726	27.816264899630106	29.41295959113921	24.47759396758696
4	12.541053250695269	17.76017998853574	36.01008505486167	33.68868170590732
5	13.322334916004412	37.854193042301176	33.621370452977814	15.202101588716596
6	30.675160378552775	35.43854641298657	18.7351097459136	15.15118346254706
7	26.391354723685197	32.428634196548465	22.91931928591049	18.260691793855845
8	28.206138129592144	31.56255702637151	20.899117051132933	19.332187792903408
9	28.020272207194445	14.73372101972005	19.708766397107677	37.537240375977824
10	17.20604668010422	28.274567358174906	30.70847616929434	23.810909792426536
11	32.610013467358854	24.01064819509749	21.79440269041349	21.58493564713016
12	23.8352121160692	26.42660480700643	29.165810383113723	20.572372693810646
13	29.72244988344753	21.079782910320294	25.08865346533988	24.10911374089229
14	24.32190558741916	20.95719234600299	26.204574061032677	28.516328005545173
15	26.46034339728259	25.473296564674374	25.330572168877257	22.735787869165776
16	23.15745823538396	26.605497419277985	26.970464724867764	23.266579620470296
17	23.393369905824436	26.432535515361288	27.094444397772044	23.079650181042233
18	24.297534520352958	24.006578129869	29.927449518675576	21.768437831102467
19	23.555151961932996	26.86318666310535	29.29512203913288	20.286539335828778
20	24.75170827896723	23.682169793645446	29.83668743850994	21.729434488877388
21	24.20525935061517	25.447468868945844	27.54349056422287	22.803781216216116
22	23.731419720135836	25.41564363499137	26.802892228790125	24.05004441608267
23	21.791842932261833	26.290851486865606	27.552166311761788	24.365139269110774
24	22.51743203058017	27.974525822389783	26.93761802040414	22.570424126625905
25	23.79890834595795	24.172883197991172	26.725830973078985	25.302377482971888
26	23.64680109227492	24.830656141979947	27.869374241924934	23.6531685238202
27	24.132208257248834	24.954634932177544	26.58563513447214	24.32752167610148
28	23.00294973801995	24.52763490286144	28.83225472537311	23.637160633745495
29	23.356730385212945	24.233086520646797	29.570166541017283	22.840016553122975
30	23.54544502224601	25.596531835318633	29.05985777977749	21.79816536265787
31	24.928144171862893	24.123849514955833	27.196707414022725	23.75129889915855
32	21.521216583497733	25.408188594466708	28.968947417716933	24.101647404318623
33	22.98310139699419	25.669029039307766	28.137818772462253	23.21005079123579
34	21.69924409567351	25.227088834215156	28.64337585743193	24.430291212679407
35	22.598124987188633	26.415668612202204	28.932736712890637	22.053469687718525
36	23.974005050429103	25.349469481481268	27.629975377918992	23.04655009017063
37	24.06324550812389	25.855829360580156	27.89481337405505	22.186111757240905
38	24.370817206499893	25.13031198339279	27.26736718082942	23.231503629277896
39	24.244009795233644	24.14009422216308	27.58766495515559	24.028231027447692
40	23.744207806731488	25.822021415174433	27.223379769449746	23.210391008644333
41	21.89604083542962	26.23738017185809	27.53113387307086	24.335445119641427
42	25.193727235647877	26.32179316411	27.222197719017693	21.262281881224425
43	24.856681757808595	23.775991894637002	26.688562684246207	24.678763663308196
44	23.403469760045297	25.569963301341893	28.00858220192789	23.017984736684923
45	25.930494660221036	24.12589717431186	27.149080596027808	22.794527569439296
46	22.416440703257525	25.102465529436234	29.831444214573978	22.649649552732264
47	24.70438477478409	24.904894673522307	28.087332229988665	22.30338832170494
48	24.53675856661706	26.13306188893626	27.171635650387927	22.15854389405876
49	23.263352946345268	25.569938580319455	29.452523613487298	21.714184859847975
50	23.74988956844187	26.27251586099497	26.191367560901956	23.786227009661207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1524.0
1	1782.5
2	2041.0
3	6401.5
4	10762.0
5	12457.0
6	14152.0
7	12556.0
8	10960.0
9	14413.5
10	17867.0
11	25047.5
12	32228.0
13	59070.0
14	85912.0
15	106857.0
16	127802.0
17	118497.5
18	109193.0
19	92885.5
20	76578.0
21	80316.0
22	84054.0
23	92090.5
24	100127.0
25	114689.5
26	129252.0
27	143580.0
28	157908.0
29	179920.0
30	201932.0
31	236164.5
32	270397.0
33	294923.0
34	319449.0
35	351987.5
36	384526.0
37	416501.0
38	448476.0
39	475746.0
40	503016.0
41	528502.0
42	553988.0
43	569201.5
44	584415.0
45	684646.0
46	784877.0
47	993264.5
48	1201652.0
49	1228833.0
50	1256014.0
51	1067738.0
52	879462.0
53	747330.0
54	615198.0
55	622136.5
56	629075.0
57	594808.5
58	560542.0
59	535582.0
60	510622.0
61	437113.5
62	363605.0
63	317067.5
64	270530.0
65	228642.5
66	186755.0
67	161974.0
68	137193.0
69	116505.5
70	95818.0
71	78383.0
72	60948.0
73	55404.0
74	49860.0
75	38276.5
76	26693.0
77	22149.5
78	17606.0
79	14554.5
80	11503.0
81	10205.5
82	8908.0
83	8049.0
84	7190.0
85	5663.0
86	4136.0
87	3890.0
88	3644.0
89	1895.5
90	147.0
91	115.0
92	83.0
93	49.0
94	15.0
95	7.5
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033698225296778246
2	0.0
3	3.190960780656799E-4
4	0.0027291111939827884
5	0.03780448798557081
6	0.03745180284665611
7	0.06437343511714479
8	0.052491304841804344
9	0.05235694859840826
10	0.060174802511017415
11	0.0548173473055989
12	0.053986018049585686
13	0.04187716161351436
14	0.04156646280066093
15	0.06197181726643994
16	0.04518568410714272
17	0.044832998968228024
18	0.051794331829187204
19	0.05607693708743712
20	0.05297834622411512
21	0.04072673627943545
22	0.044446724768464306
23	0.061535159475402695
24	0.04179318896139181
25	0.050106481521524
26	0.0361922130648179
27	0.0437245599602104
28	0.04089468158368055
29	0.043203929517050606
30	0.039517530088870774
31	0.04442153297282754
32	0.03488223969170617
33	0.03546165099135174
34	0.03767852900738699
35	0.043220724047475116
36	0.04279246352165012
37	0.03716629582943945
38	0.04919117961338823
39	0.047923192566337765
40	0.042968806091107474
41	0.04573150634593928
42	0.04563073916339222
43	0.045420807533085854
44	0.041432106557264856
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0389381187892252
50	0.0068101820871385894
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.1908639E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.12428128712538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.96000485309386	18.550777301885667
2	16.16034914529746	9.08996410121004
3	6.911468480146345	5.831402509282102
4	3.3674257451303653	3.7882571547821673
5	1.9736533588435	2.7753791113697197
6	1.206042151463761	2.0351441227138016
7	0.8215155173570953	1.6173173444322497
8	0.5664535352656425	1.2744878849518022
9	0.41284755163522235	1.0449936603801013
>10	2.1865949185607394	11.27043819193297
>50	0.20511163630433232	4.031541502533206
>100	0.18117305122635957	10.633658413305985
>500	0.02673337429114211	5.182820318330331
>1k	0.01812007173484418	9.560332599570966
>5k	0.0013590052315050822	2.5274473160493924
>10k+	0.0011476044177154028	10.786038467269552
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	143827	1.2077534636829617	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	107294	0.9009761736836593	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	87132	0.7316705124741795	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	72448	0.6083650700974309	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71976	0.6044015609172467	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	65553	0.5504659264589346	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	54276	0.4557699666603379	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	52689	0.44244350676848965	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45067	0.3784395513206841	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	38508	0.3233618887935053	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37492	0.31483026733785446	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	35848	0.30102516332890766	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	34391	0.2887903479146526	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	33555	0.2817702341972076	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	29179	0.2450238016283809	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	28402	0.23849912655845895	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	23335	0.19595018372796422	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	22054	0.1851932869910659	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	21581	0.18122138054566939	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	20784	0.17452876017150237	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	18762	0.15754948991232332	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	17895	0.15026906097329848	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	17118	0.14374438590337654	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	16431	0.13797546470255753	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	15978	0.13417150356140614	No Hit
GTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCACTTTTTC	14636	0.12290237364656027	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	14522	0.12194508541236325	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	13255	0.1113057503884365	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	12694	0.10659488460436159	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	12607	0.10586432253089542	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	12348	0.10368943084092146	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12099	0.10159851180307003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5191795636764202E-5	0.0	0.0	0.0	0.0
2	3.358906084901893E-5	8.397265212254733E-6	0.0	0.0	0.0
3	3.358906084901893E-5	8.397265212254733E-6	0.0	1.6794530424509466E-5	0.0
4	3.358906084901893E-5	8.397265212254733E-6	0.0	2.5191795636764202E-5	0.0
5	4.198632606127367E-5	8.397265212254733E-6	0.0	2.5191795636764202E-5	0.0
6	4.198632606127367E-5	8.397265212254733E-6	0.0	2.5191795636764202E-5	0.0
7	4.198632606127367E-5	8.397265212254733E-6	0.0	2.5191795636764202E-5	0.0
8	7.55753869102926E-5	8.397265212254733E-6	0.0	2.5191795636764202E-5	0.0
9	9.236991733480207E-5	8.397265212254733E-6	0.0	6.717812169803786E-5	0.0
10	1.4275350860833047E-4	8.397265212254733E-6	0.0	1.3435624339607573E-4	0.0
11	2.6871248679215145E-4	8.397265212254733E-6	0.0	1.511507738205852E-4	0.0
12	2.939042824289157E-4	8.397265212254733E-6	0.0	1.763425694573494E-4	0.0
13	2.939042824289157E-4	1.6794530424509466E-5	0.0	1.8473983466960414E-4	0.0
14	3.106988128534251E-4	1.6794530424509466E-5	0.0	2.267261607308778E-4	0.0
15	3.106988128534251E-4	1.6794530424509466E-5	0.0	2.6031522157989674E-4	0.0
16	4.1986326061273666E-4	1.6794530424509466E-5	0.0	3.106988128534251E-4	8.397265212254733E-6
17	4.450550562495009E-4	1.6794530424509466E-5	0.0	3.6108240412695356E-4	8.397265212254733E-6
18	4.534523214617556E-4	1.6794530424509466E-5	0.0	4.1146599540048195E-4	8.397265212254733E-6
19	4.6184958667401033E-4	1.6794530424509466E-5	0.0	5.206304431597935E-4	8.397265212254733E-6
20	4.6184958667401033E-4	1.6794530424509466E-5	0.0	6.549866865558692E-4	8.397265212254733E-6
21	4.954386475230293E-4	1.6794530424509466E-5	0.0	8.565210516499829E-4	8.397265212254733E-6
22	5.038359127352841E-4	1.6794530424509466E-5	0.0	0.0011588225992911533	8.397265212254733E-6
23	5.542195040088124E-4	1.6794530424509466E-5	0.0	0.0015870831251161446	8.397265212254733E-6
24	5.794112996455766E-4	2.5191795636764202E-5	0.0	0.002116110833488193	8.397265212254733E-6
25	5.794112996455766E-4	5.0383591273528404E-5	0.0	0.002334439729006816	8.397265212254733E-6
26	7.473566038906713E-4	5.0383591273528404E-5	0.0	0.002779494785256317	8.397265212254733E-6
27	7.725483995274355E-4	5.0383591273528404E-5	0.0	0.0032413443719303274	8.397265212254733E-6
28	7.809456647396903E-4	5.878085648578314E-5	0.0	0.00535745520541852	8.397265212254733E-6
29	8.229319908009639E-4	5.878085648578314E-5	0.0	0.00832168982534444	8.397265212254733E-6
30	8.481237864377281E-4	6.717812169803786E-5	0.0	0.012990569283358074	8.397265212254733E-6
31	8.649183168622376E-4	7.55753869102926E-5	0.0	0.018658723301630018	8.397265212254733E-6
32	8.81712847286747E-4	7.55753869102926E-5	0.0	0.02471315151966568	8.397265212254733E-6
33	8.985073777112565E-4	7.55753869102926E-5	0.0	0.03154012813722878	8.397265212254733E-6
34	9.236991733480207E-4	7.55753869102926E-5	0.0	0.03928240666292764	8.397265212254733E-6
35	9.572882341970396E-4	7.55753869102926E-5	0.0	0.04995533074770341	8.397265212254733E-6
36	9.656854994092944E-4	7.55753869102926E-5	0.0	0.0628871191745757	8.397265212254733E-6
37	0.0010328636211073322	7.55753869102926E-5	0.0	0.08035343081606555	8.397265212254733E-6
38	0.0010580554167440965	9.236991733480207E-5	0.0	0.1008427579339671	8.397265212254733E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACG	1095	0.0	17.478899	22
AATGCGA	1140	0.0	17.175398	20
AGTCGAC	1265	0.0	16.520174	35
GTACTAG	1485	0.0	16.451803	1
CGACGAA	1265	0.0	16.346071	38
TCGACGA	1285	0.0	16.262846	37
CAATGCG	1240	0.0	16.146278	19
CGACGAG	1390	0.0	16.14161	24
GTCGACG	1295	0.0	15.967397	36
GTATTAG	2255	0.0	15.9095545	1
GCGTCGG	1715	0.0	15.39588	9
CAATTCG	1365	0.0	15.151294	15
TTAACGG	885	0.0	14.913841	35
GACCGTC	1730	0.0	14.87814	22
AGACCGT	1720	0.0	14.83674	21
GTATTAA	2170	0.0	14.808467	1
GGCGTCG	1790	0.0	14.626954	8
GTCGGCT	1760	0.0	14.623122	26
CGAACAA	1435	0.0	14.561673	41
CGTCGTA	2300	0.0	14.349904	10
>>END_MODULE