FastQCFastQC Report
Wed 25 May 2016
SRR2558128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9135358
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA569940.6238835960232757No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT445260.487402901999024No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC359570.39360252767324494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326000.3568551993255218No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA304900.3337581296759251No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG257790.28218926942983513No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG216960.2374947976860896No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG207390.2270190177549692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199940.21886389126731542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169750.18581647265493043No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA156790.1716298365099649No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT143100.15664410743399437No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC137780.15082058086831407No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC137460.1504702935560927No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA122830.1344555955004719No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC121210.13268226598235122No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT117780.1289276238544784No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC115820.1267821140671225No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG106490.11656904962016813No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA101830.11146799063594443No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG100960.11051564700584257No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT96770.10592907251144398No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG93340.10217443038357119No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA93230.10205401911999508No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT93100.10191171489940515No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG91390.10003986707472219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA31550.019.03944810
CCGTCGT31900.018.6236239
TACCGTC33300.018.2375077
ATACCGT34650.017.9714786
ACCGTCG34500.017.4105788
GGCGTTA31050.017.35555842
GTCGTAG34750.017.34844811
TCTAGAC17650.017.3276543
CTAGACA20250.015.9740244
CGGCGTT34600.015.829441
GTTCTAG19750.015.8251551
GCGGCGT35050.015.43824140
GGTATAG29900.015.3851521
GTACTAG6900.014.6735581
CCTTACC46750.014.49187528
GTAGACC33000.014.46997454
ATGCGGC37550.014.46968835
GAATCTA36400.014.44558813
GCGTTAT38350.014.22894343
TCTACCA50650.014.2048932
Produced by FastQC (version 0.11.5)