##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9135358
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04325205427089	33.0	32.0	34.0	28.0	34.0
2	32.09085697572005	33.0	33.0	34.0	28.0	34.0
3	32.08450779925647	33.0	33.0	34.0	28.0	34.0
4	32.04632297935122	33.0	33.0	34.0	28.0	34.0
5	32.0682302762519	33.0	33.0	34.0	30.0	34.0
6	35.59440735655899	38.0	36.0	38.0	29.0	38.0
7	35.701665003166816	38.0	37.0	38.0	30.0	38.0
8	35.68558780071892	38.0	37.0	38.0	29.0	38.0
9	35.84006352022548	38.0	37.0	38.0	30.0	38.0
10	35.779457137859296	38.0	37.0	38.0	29.0	38.0
11	35.85144271302778	38.0	37.0	38.0	31.0	38.0
12	35.79269832665562	38.0	37.0	38.0	29.0	38.0
13	35.83777526835839	38.0	37.0	38.0	31.0	38.0
14	35.846710988228374	38.0	37.0	38.0	31.0	38.0
15	35.807390909037174	38.0	37.0	38.0	29.0	38.0
16	35.80498224590651	38.0	37.0	38.0	29.0	38.0
17	35.76189044808096	38.0	37.0	38.0	29.0	38.0
18	35.77582597200898	38.0	37.0	38.0	29.0	38.0
19	35.693997761226214	38.0	37.0	38.0	29.0	38.0
20	35.693060852130806	38.0	37.0	38.0	29.0	38.0
21	35.71352047724895	38.0	37.0	38.0	29.0	38.0
22	35.73770672150999	38.0	37.0	38.0	29.0	38.0
23	35.73090928675154	38.0	37.0	38.0	29.0	38.0
24	35.631002966714604	38.0	37.0	38.0	29.0	38.0
25	35.69697334247875	38.0	37.0	38.0	29.0	38.0
26	35.65310226484829	38.0	37.0	38.0	29.0	38.0
27	35.657070144377485	38.0	37.0	38.0	29.0	38.0
28	35.64047604921449	38.0	37.0	38.0	29.0	38.0
29	35.685114365523496	38.0	37.0	38.0	29.0	38.0
30	35.661836241119396	38.0	37.0	38.0	29.0	38.0
31	35.71665182689064	38.0	37.0	38.0	29.0	38.0
32	35.73359018880267	38.0	37.0	38.0	29.0	38.0
33	35.68833668040158	38.0	37.0	38.0	29.0	38.0
34	35.69986113297366	38.0	37.0	38.0	29.0	38.0
35	35.62514692910776	38.0	37.0	38.0	29.0	38.0
36	35.63757851635371	38.0	37.0	38.0	29.0	38.0
37	35.58936748838962	38.0	37.0	38.0	29.0	38.0
38	35.56846628232851	38.0	37.0	38.0	29.0	38.0
39	35.56313359585908	38.0	37.0	38.0	29.0	38.0
40	35.54689372874057	38.0	37.0	38.0	29.0	38.0
41	35.53854627262555	38.0	37.0	38.0	29.0	38.0
42	35.50229427243026	38.0	37.0	38.0	28.0	38.0
43	35.54827944345476	38.0	37.0	38.0	29.0	38.0
44	35.50030956641218	38.0	37.0	38.0	28.0	38.0
45	35.535333590648555	38.0	37.0	38.0	29.0	38.0
46	35.51289615579378	38.0	37.0	38.0	28.0	38.0
47	35.418571007288385	38.0	37.0	38.0	28.0	38.0
48	35.43422885014468	38.0	37.0	38.0	28.0	38.0
49	35.330224825343464	38.0	37.0	38.0	27.0	38.0
50	35.013427388395726	38.0	36.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	180.0
5	503.0
6	1051.0
7	482.0
8	272.0
9	215.0
10	176.0
11	154.0
12	139.0
13	148.0
14	125.0
15	132.0
16	313.0
17	1401.0
18	7941.0
19	24426.0
20	42268.0
21	54681.0
22	62805.0
23	70542.0
24	80044.0
25	91667.0
26	103197.0
27	117599.0
28	135470.0
29	157098.0
30	182890.0
31	212398.0
32	254869.0
33	316370.0
34	417942.0
35	630587.0
36	1331760.0
37	4835504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44155578229192	18.123422253261598	13.212306046787262	25.222715917659222
2	15.794969392551447	20.882476636383597	37.46377536600099	25.85877860506397
3	18.25647639581195	25.868091435797382	29.348360562423153	26.52707160596751
4	13.156377754818902	16.98664962484568	35.78068682654539	34.07628579379002
5	14.770343984420043	36.44693062877748	33.24849475676572	15.534230630036754
6	32.567538371965824	35.19105307310255	17.721519541782296	14.519889013149328
7	28.188129335495322	30.79812325873715	22.427750324539193	18.585997081228335
8	27.773446308564232	31.66117813115119	20.71703940848449	19.84833615180009
9	27.775201671857452	14.16359706062542	19.60439482453141	38.456806442985716
10	17.38117800342946	27.089976053350295	30.057853546040565	25.470992397179675
11	34.481912293559816	22.836591852657595	22.149648023838616	20.531847829943974
12	24.53568126931182	24.866081544513957	28.707335208098616	21.890901978075608
13	29.64161940354732	20.132479092968612	25.10452420625117	25.121377297232893
14	24.69982809775016	20.20485474380345	25.193294213471102	29.902022944975293
15	25.912350597609564	26.259607617639407	23.449930860749443	24.37811092400159
16	24.80139998214979	25.56292500338114	25.2357733548923	24.399901659576777
17	23.66761535648802	26.201316951870446	26.127736819010067	24.003330872631466
18	25.167260579991463	23.939350530645072	27.962858677979384	22.930530211384077
19	24.942047045335716	25.603268189605117	26.887936739008104	22.566748026051066
20	25.57997899657328	23.66505086962488	27.102711005968636	23.652259127833197
21	26.00011432506163	24.520557485794782	26.118053109904494	23.361275079239093
22	25.169088918398185	24.23553805439712	25.66788507494755	24.927487952257145
23	23.229630609703843	24.766694063310783	26.334874467583607	25.668800859401763
24	23.74899869412741	26.22768176131277	26.05318214048123	23.970137404078592
25	25.08282638721878	23.733948896677614	25.499750962310546	25.683473753793063
26	24.780314997359948	24.716114963940452	26.25230580283768	24.25126423586192
27	25.20994875724857	24.23857813821471	25.60971296152616	24.941760143010566
28	23.988726585308882	24.213202755562982	26.88136906920084	24.916701589927296
29	24.18991836599708	23.880491919716455	27.42537178590616	24.504217928380307
30	24.364893241442626	24.63803141652964	27.565470814305026	23.431604527722712
31	25.782229429435745	23.797865695269785	25.833162497842654	24.586742377451817
32	23.790800405499247	24.321096451049367	26.637003930819848	25.251099212631534
33	24.033485173667383	24.62396522272349	26.331884280145417	25.01066532346371
34	23.823695018439153	24.206133068950102	26.530272339693028	25.439899572917714
35	24.563609237423297	24.69002567313686	27.180683516058963	23.565681573380886
36	24.704096345802725	24.42836675856923	26.34833345068915	24.519203444938896
37	25.303916902989904	24.83172303566278	26.540291314335345	23.32406874701197
38	24.932632325488537	24.08357950305512	26.49259672898537	24.491191442470974
39	25.493024518523622	23.570382488179995	26.05401550549914	24.882577487797246
40	25.198050323212257	24.976680314988222	25.601389434906924	24.223879926892597
41	23.991247835093095	25.055579861040766	26.261915543579857	24.691256760286283
42	26.23423690195283	25.112596099691036	25.792591452059582	22.860575546296552
43	25.503998952200384	23.60723853440781	25.484933116808524	25.403829396583284
44	24.661816402635427	24.818169852102645	25.857222734347847	24.662791010914077
45	25.802896832286155	24.001117416525986	25.539940525592975	24.65604522559488
46	23.82068661129646	24.564598344148088	27.367093878532184	24.24762116602327
47	25.24717695792546	24.275939705920667	26.475284274573585	24.00159906158029
48	25.67023645926082	25.168581242245786	25.409600806011106	23.751581492482288
49	24.200794037361103	25.184208082641558	26.992528742680733	23.6224691373166
50	25.047190409511934	25.410033562854846	25.109753348759323	24.433022678873904
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	882.0
1	1051.0
2	1220.0
3	3317.5
4	5415.0
5	6092.5
6	6770.0
7	5988.5
8	5207.0
9	6733.0
10	8259.0
11	11353.0
12	14447.0
13	27116.5
14	39786.0
15	49317.0
16	58848.0
17	54979.5
18	51111.0
19	44331.0
20	37551.0
21	41832.5
22	46114.0
23	51221.0
24	56328.0
25	66204.0
26	76080.0
27	86566.0
28	97052.0
29	114092.5
30	131133.0
31	147270.0
32	163407.0
33	179777.5
34	196148.0
35	222962.0
36	249776.0
37	283564.5
38	317353.0
39	353106.5
40	388860.0
41	412860.0
42	436860.0
43	446544.5
44	456229.0
45	516196.5
46	576164.0
47	697214.0
48	818264.0
49	841314.0
50	864364.0
51	751435.0
52	638506.0
53	572738.0
54	506970.0
55	532279.0
56	557588.0
57	541673.5
58	525759.0
59	495781.5
60	465804.0
61	414483.5
62	363163.0
63	322236.5
64	281310.0
65	241602.5
66	201895.0
67	180763.0
68	159631.0
69	137334.0
70	115037.0
71	93146.5
72	71256.0
73	62911.5
74	54567.0
75	41757.0
76	28947.0
77	23693.5
78	18440.0
79	15356.0
80	12272.0
81	11270.5
82	10269.0
83	9668.0
84	9067.0
85	7393.5
86	5720.0
87	5463.5
88	5207.0
89	2700.0
90	193.0
91	152.5
92	112.0
93	61.5
94	11.0
95	7.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03143828627186805
2	0.0
3	3.283943552075354E-4
4	0.0020141520452728835
5	0.034076387592035254
6	0.03398881576397991
7	0.06009616700297898
8	0.04933577863067873
9	0.04855857865668757
10	0.05541107420201813
11	0.050638409573001954
12	0.05051799830942586
13	0.038334567731226295
14	0.03748074240768671
15	0.057282922026701086
16	0.04173892254687775
17	0.04178270846090542
18	0.04812071951641085
19	0.051185733498347846
20	0.048897919490402016
21	0.038334567731226295
22	0.04129011692809412
23	0.05786308538756773
24	0.03867390856494075
25	0.04608467451412413
26	0.032445362294504496
27	0.040775632438268976
28	0.03755736775723513
29	0.03967003810907027
30	0.036670702998174785
31	0.04122443805705261
32	0.031876145412144764
33	0.032379683423462986
34	0.034590872081860395
35	0.03977950289413945
36	0.03924312544730048
37	0.033846511543389975
38	0.0451432773625292
39	0.04397200416228899
40	0.03927596488282123
41	0.042724105612500354
42	0.04187028028896076
43	0.04204542394507145
44	0.038137531118101774
45	0.0
46	0.0
47	0.0
48	0.0
49	0.036057700201787383
50	0.00609718852835324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9135358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.440257091956575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49293642616004	24.910629456255688
2	13.326140328156772	8.912591172341061
3	4.648098204388935	4.663007968302832
4	2.19433826055448	2.935169423186344
5	1.1812092018201765	1.9749969694125766
6	0.764882137036395	1.5346713184525322
7	0.5003325444202125	1.171187424181928
8	0.3955682173391378	1.058232230818218
9	0.29287910769955916	0.8814557392502486
>10	1.8079809381540837	11.55307617418016
>50	0.19518671309927513	4.571339145773274
>100	0.15959268783643596	10.843270592568164
>500	0.02153790687875348	4.979753187808548
>1k	0.01716359366331239	11.808093343193693
>5k	0.0014579114289072815	3.305570739729874
>10k+	6.958213637966571E-4	4.896955114544974
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	56994	0.6238835960232757	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	44526	0.487402901999024	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	35957	0.39360252767324494	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32600	0.3568551993255218	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	30490	0.3337581296759251	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	25779	0.28218926942983513	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	21696	0.2374947976860896	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	20739	0.2270190177549692	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19994	0.21886389126731542	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16975	0.18581647265493043	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	15679	0.1716298365099649	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	14310	0.15664410743399437	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	13778	0.15082058086831407	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13746	0.1504702935560927	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	12283	0.1344555955004719	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	12121	0.13268226598235122	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	11778	0.1289276238544784	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	11582	0.1267821140671225	No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG	10649	0.11656904962016813	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	10183	0.11146799063594443	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG	10096	0.11051564700584257	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT	9677	0.10592907251144398	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	9334	0.10217443038357119	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	9323	0.10205401911999508	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	9310	0.10191171489940515	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	9139	0.10003986707472219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.473239253458923E-5	0.0	0.0	0.0	0.0
2	5.473239253458923E-5	0.0	0.0	0.0	0.0
3	5.473239253458923E-5	0.0	0.0	1.0946478506917846E-5	0.0
4	5.473239253458923E-5	0.0	0.0	1.0946478506917846E-5	0.0
5	5.473239253458923E-5	0.0	0.0	1.0946478506917846E-5	0.0
6	8.757182805534277E-5	0.0	0.0	1.0946478506917846E-5	1.0946478506917846E-5
7	8.757182805534277E-5	0.0	0.0	1.0946478506917846E-5	1.0946478506917846E-5
8	8.757182805534277E-5	0.0	0.0	1.0946478506917846E-5	1.0946478506917846E-5
9	8.757182805534277E-5	0.0	0.0	4.378591402767138E-5	1.0946478506917846E-5
10	9.851830656226061E-5	0.0	0.0	6.567887104150707E-5	2.189295701383569E-5
11	1.641971776037677E-4	0.0	0.0	7.662534954842492E-5	2.189295701383569E-5
12	1.641971776037677E-4	0.0	0.0	7.662534954842492E-5	2.189295701383569E-5
13	1.641971776037677E-4	1.0946478506917846E-5	0.0	7.662534954842492E-5	2.189295701383569E-5
14	1.641971776037677E-4	1.0946478506917846E-5	0.0	9.851830656226061E-5	2.189295701383569E-5
15	1.641971776037677E-4	1.0946478506917846E-5	0.0	1.5325069909684984E-4	2.189295701383569E-5
16	2.0798309163143908E-4	1.0946478506917846E-5	0.0	1.641971776037677E-4	2.189295701383569E-5
17	2.189295701383569E-4	1.0946478506917846E-5	0.0	1.9703661312452122E-4	2.189295701383569E-5
18	2.189295701383569E-4	1.0946478506917846E-5	0.0	2.0798309163143908E-4	2.189295701383569E-5
19	2.189295701383569E-4	1.0946478506917846E-5	0.0	2.7366196267294615E-4	2.189295701383569E-5
20	2.189295701383569E-4	1.0946478506917846E-5	0.0	2.955549196867818E-4	2.189295701383569E-5
21	2.5176900565911044E-4	1.0946478506917846E-5	0.0	3.831267477421246E-4	6.567887104150707E-5
22	2.5176900565911044E-4	1.0946478506917846E-5	0.0	4.925915328113031E-4	6.567887104150707E-5
23	2.7366196267294615E-4	1.0946478506917846E-5	0.0	6.348957534012351E-4	6.567887104150707E-5
24	2.84608441179864E-4	1.0946478506917846E-5	0.0	8.319323665257563E-4	6.567887104150707E-5
25	2.84608441179864E-4	1.0946478506917846E-5	0.0	8.757182805534277E-4	6.567887104150707E-5
26	3.393408337144532E-4	1.0946478506917846E-5	0.0	0.0011055943291987025	6.567887104150707E-5
27	3.612337907282889E-4	2.189295701383569E-5	0.0	0.0013902027703785664	7.662534954842492E-5
28	3.612337907282889E-4	3.283943552075354E-5	0.0	0.002298760486452748	7.662534954842492E-5
29	3.831267477421246E-4	3.283943552075354E-5	0.0	0.003426247772665286	7.662534954842492E-5
30	3.9407322624904244E-4	3.283943552075354E-5	0.0	0.0053637744683897445	7.662534954842492E-5
31	4.1596618326287816E-4	3.283943552075354E-5	0.0	0.008461627885847495	7.662534954842492E-5
32	4.26912661769796E-4	3.283943552075354E-5	0.0	0.011471909475249903	7.662534954842492E-5
33	4.488056187836317E-4	3.283943552075354E-5	0.0	0.014952889640449777	9.851830656226061E-5
34	4.488056187836317E-4	3.283943552075354E-5	0.0	0.019232962736654657	9.851830656226061E-5
35	4.816450543043852E-4	3.283943552075354E-5	0.0	0.024968917474279605	9.851830656226061E-5
36	5.035380113182209E-4	3.283943552075354E-5	0.0	0.03116462430919511	9.851830656226061E-5
37	5.254309683320566E-4	3.283943552075354E-5	0.0	0.04006411133531931	9.851830656226061E-5
38	5.254309683320566E-4	4.378591402767138E-5	0.0	0.051251412369389356	9.851830656226061E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	3155	0.0	19.039448	10
CCGTCGT	3190	0.0	18.623623	9
TACCGTC	3330	0.0	18.237507	7
ATACCGT	3465	0.0	17.971478	6
ACCGTCG	3450	0.0	17.410578	8
GGCGTTA	3105	0.0	17.355558	42
GTCGTAG	3475	0.0	17.348448	11
TCTAGAC	1765	0.0	17.327654	3
CTAGACA	2025	0.0	15.974024	4
CGGCGTT	3460	0.0	15.8294	41
GTTCTAG	1975	0.0	15.825155	1
GCGGCGT	3505	0.0	15.438241	40
GGTATAG	2990	0.0	15.385152	1
GTACTAG	690	0.0	14.673558	1
CCTTACC	4675	0.0	14.491875	28
GTAGACC	3300	0.0	14.4699745	4
ATGCGGC	3755	0.0	14.469688	35
GAATCTA	3640	0.0	14.445588	13
GCGTTAT	3835	0.0	14.228943	43
TCTACCA	5065	0.0	14.204893	2
>>END_MODULE