FastQCFastQC Report
Wed 25 May 2016
SRR2558127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15797325
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5353923.3891307547322094No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3996142.5296308077475143No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC3480582.203271756452437No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2947391.8657525878590204No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2588781.6387458003174589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2094631.3259396764958624No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2015431.2758046061595871No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1968451.246065393982842No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA1549810.9810585019932172No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1377320.8718691297419026No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1291850.8177650330040054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1257670.7961284584573655No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1250070.7913175173644905No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1153750.7303451691979496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1096730.6942504506300908No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1054910.6677776142479819No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG905720.5733375745577178No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG869720.5505489062230472No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC841360.5325964997238457No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT837180.5299504821227645No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA718660.45492512181651No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT711470.4503737183352245No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA677330.4287624645311785No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC609170.3856159191508689No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG602050.3811088269691229No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC552960.3500339456205402No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG530580.3358669901391533No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG512960.3247132030264617No Hit
GTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCACTTTTTC509290.32239002489345503No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG492170.3115527470631895No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA468400.29650589577665837No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC435400.2756162831365437No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA434830.27525546255457806No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG428050.2709635966848818No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA416260.26350030780527717No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA392050.2481749283502112No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT391870.24806098500853782No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369840.23411558602484916No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG360520.22821585300042885No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG351490.22249969535981565No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC346410.21928396105036768No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC343750.21760013166786152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA343390.2173722449845148No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC309500.19591924582168185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG293750.18594920342526344No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT286800.18154972439954234No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA282800.1790176501401345No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA280890.17780858468126726No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG265390.16799679692606184No Hit
GTATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCT261320.16542041136711438No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA260060.1646228079754009No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC258130.16340108214523663No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA244180.15457047316555175No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG241500.15287398341174852No Hit
GTACATGGGACTGCTGCTAGCTCCTCGCCCTTGCTCACTTTTTCGAACTG225700.14287229008708752No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA222680.1409605740212346No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC219160.1387323486729557No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC210130.1330161910323425No Hit
TATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTT206890.13096521088222216No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT206730.13086392791184584No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT199200.1260972981185106No Hit
GTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAG194530.12314110142065192No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT190660.12069131957467483No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG187270.1185453866398267No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA185620.11750090600782094No Hit
GTATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGT185200.11723503821058313No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC184590.11684889688602343No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA182310.11540561455816095No Hit
ACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCA176990.11203795579314851No Hit
GTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAAAAAA174460.11043641882407305No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG172960.10948689097679512No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171930.10883488185499761No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171150.10834112737441307No Hit
CTCCTCGCCCTTGCTCACTTTTTCGAACTGCGGGTGGCTCCAGCTAGCAG170050.10764480695307592No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG168610.1067332602196891No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG166390.10532795900571774No Hit
GCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGA163640.10358715795237484No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161560.10227047933748278No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT160030.10130196093325926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAT6150.029.33349816
CAATGCG26450.027.8657319
CGACGAG26450.027.36230724
GTCATCC27150.026.82151213
ATCGTCA27950.026.29303410
GTCGTCA28150.025.24100124
GTCAGCG28950.024.8549243
CGCCAGC29250.024.44187234
CGTCATT30350.023.5560826
AATGCGA32050.023.5446720
GTACTAG35250.023.163031
CATTGCG31300.023.12114329
TGCGACG31550.023.08073422
GACGAGT32400.022.81364325
CGCGGTA22250.022.64741543
TCGTCAG33400.021.6014941
TAGGCAT45500.021.5206265
TCAGCGC33700.021.48079144
CGTCATC34650.021.33358812
GGCCGCG23700.021.25474440
Produced by FastQC (version 0.11.5)