FastQCFastQC Report
Wed 25 May 2016
SRR2558122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682274
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA855020.8830776736952497No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT743860.768269933282202No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA585130.6043311726150282No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC579190.5981962501784188No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG357660.3693966933800882No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG343040.3542969347903189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338720.3498351730182393No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG309610.3197699218179531No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA264850.2735411123461286No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC245460.2535148251330215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224570.23193931508238663No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC215480.2225510246869692No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT214150.22117738043769467No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC210320.21722169812587416No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC205620.2123674665682876No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT200110.20667665467843607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197510.20399133509338818No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG188060.19423123121696412No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC168930.17447347596236173No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTT164630.17003237049478254No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG140640.14525513324659062No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA133810.13820100525971482No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG125110.12921551280205457No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGCAGAAATG124820.1289159963867992No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT118500.12238860416468281No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTA117120.12096331915415737No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA115000.11877375087711833No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG108650.11221537419825137No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTC106830.11033565048871782No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTCATCTCAT105890.10936480417720051No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC105050.10849723938818505No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC103760.10716490774791128No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATC98530.10176328412106495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG11450.017.87043219
GTACTAG15450.017.0930791
CGACGAG12150.016.8376424
CGACGAA9150.016.82925638
AATGCGA12000.016.68329420
CGTCGTA16800.016.50217810
GTCGACG9600.016.49859636
TCGACGA9700.016.32859437
AGTCGAC9750.016.2449435
CAATTCG10300.016.02020815
TGCGACG12300.015.91841422
CCGTCGT17700.015.7873959
ATCGTCA13550.015.101624510
GACGAGT13800.014.98454325
CGAACAA11000.014.59808141
AGGTCGC29950.014.54616110
TCTCGCC33400.014.22824812
CGGCCTT34450.014.04778924
CATTGCG10200.014.0179129
AAGTCGA11750.013.85392734
Produced by FastQC (version 0.11.5)