FastQCFastQC Report
Wed 25 May 2016
SRR2558120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163129
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA584770.7163552113411414No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT485940.5952864397953285No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC381750.4676515586118999No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA354030.4336939916054249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268350.32873423903995636No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG259210.3175375520832759No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG229550.28120344539452946No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG224630.2751763447570166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186390.22833156256626594No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA179520.2199156720419339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158760.19448424740072098No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT156970.1922914607866665No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC152050.18626436014915357No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC151980.18617860871731906No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC135510.1660025218271082No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC134060.1642262421676786No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC106450.1304034274112292No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG104030.1274388779106639No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT102360.1253930937511829No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG97570.11952524577279128No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA88420.10831630861156305No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA87210.10683403386128039No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT86800.10633177547482099No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG86420.10586626770200495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA27950.016.29520210
CCGTCGT28350.016.1429969
TACCGTC30200.015.809527
ATACCGT32100.015.0108396
GGCGTTA26400.014.99809142
GTACTAG7500.014.9654311
GTCGTAG30500.014.93217511
AATGCGA5600.014.92877520
CTAACGC3600.014.66803553
ACCGTCG32050.014.5533528
TGCGACG5850.014.2905322
TCTAACG3750.014.0813142
TACGACG17050.013.8089255
ACGACGG16450.013.64269356
GCATTCG36650.013.44604822
CGGCGTT29700.013.25765341
CGACGGT16950.013.2402547
CGTTATT31050.013.10959644
ATTCGTA37700.013.07075524
GCGTTAT31250.013.02601443
Produced by FastQC (version 0.11.5)