FastQCFastQC Report
Wed 25 May 2016
SRR2558117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7979085
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA429690.5385203942557324No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT370630.46450188210803617No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC260220.3261276198962663No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA249730.3129807490457866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236910.29691374386912783No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG203680.2552673646163689No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG174800.21907273829016735No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG166180.2082694945598399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155070.19434559225775888No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG147280.18458256805134926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138890.17406757792403513No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT123050.1542156776121573No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA120250.1507065033146031No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC117720.14753571368145596No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC112800.14136959313003936No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCAGCTATGG108630.13614342997975332No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA100730.1262425453545112No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC100150.12551564496430356No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCACAGCCAT98420.12334747655902902No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC96010.12032708011006274No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG92530.11596567776881685No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT91550.11473746676467289No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT83240.10432273876014606No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTTGCAGACA81020.10154046485279954No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA80610.10102662147351482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC33500.018.9804943
CTAGACA34400.018.6128794
GGTATAG38300.018.2167221
TAGACAT36400.017.892885
GTTCTAG35400.017.7194941
CTCTAGT33550.017.57230427
CGTACGA2055.456968E-1217.17166712
TATAGAG40950.016.6556763
CTCTACC58050.016.6066341
CCTTACC57300.016.4312328
ATCCTTA57200.016.38304126
TCTACCA59000.016.370692
GTATAGA42150.016.3380892
TAGAGAC50000.016.2825185
AATCTAC45100.016.24478714
TAGACCT45500.016.1035925
GAATCTA45050.016.0181313
CGTCGTA28750.015.99511710
GTAGACC44350.015.9750164
ATACCGT30400.015.8504426
Produced by FastQC (version 0.11.5)