##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7979085
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88123851293726	33.0	32.0	34.0	18.0	34.0
2	31.06814666092666	33.0	32.0	34.0	25.0	34.0
3	31.103135259243384	33.0	32.0	34.0	25.0	34.0
4	31.019518403425955	33.0	32.0	34.0	25.0	34.0
5	31.049812729153782	33.0	32.0	34.0	25.0	34.0
6	34.091242416893664	38.0	34.0	38.0	16.0	38.0
7	34.13729368718343	38.0	34.0	38.0	16.0	38.0
8	34.09166890689847	38.0	34.0	38.0	16.0	38.0
9	34.32641261498029	38.0	34.0	38.0	26.0	38.0
10	34.25804161755389	38.0	34.0	38.0	16.0	38.0
11	34.31557039434973	38.0	34.0	38.0	26.0	38.0
12	34.23961469266213	38.0	34.0	38.0	16.0	38.0
13	34.29403622094513	38.0	34.0	38.0	26.0	38.0
14	34.313715169095204	38.0	34.0	38.0	25.0	38.0
15	34.23496942318574	38.0	34.0	38.0	16.0	38.0
16	34.22933105738315	38.0	34.0	38.0	16.0	38.0
17	34.173313857415984	38.0	34.0	38.0	16.0	38.0
18	34.20448434876931	38.0	34.0	38.0	16.0	38.0
19	34.10352277234796	38.0	34.0	38.0	16.0	38.0
20	34.102411993355126	38.0	34.0	38.0	16.0	38.0
21	34.14083519601558	38.0	34.0	38.0	16.0	38.0
22	34.170253481445556	38.0	34.0	38.0	16.0	38.0
23	34.15951340285258	38.0	34.0	38.0	16.0	38.0
24	34.0442557009983	38.0	34.0	38.0	16.0	38.0
25	34.122264896288236	38.0	34.0	38.0	16.0	38.0
26	34.07520599166446	38.0	34.0	38.0	16.0	38.0
27	34.097383973225	38.0	34.0	38.0	16.0	38.0
28	34.06716822793591	38.0	34.0	38.0	16.0	38.0
29	34.10676763062431	38.0	34.0	38.0	16.0	38.0
30	34.08398331387622	38.0	34.0	38.0	16.0	38.0
31	34.139957150475276	38.0	34.0	38.0	16.0	38.0
32	34.16231886237583	38.0	34.0	38.0	16.0	38.0
33	34.092691455223246	38.0	34.0	38.0	16.0	38.0
34	34.11119207277526	38.0	34.0	38.0	16.0	38.0
35	34.03224893581156	38.0	34.0	38.0	16.0	38.0
36	34.05308039706307	38.0	34.0	38.0	16.0	38.0
37	34.00122908829772	38.0	34.0	38.0	16.0	38.0
38	33.98548041536091	38.0	34.0	38.0	16.0	38.0
39	34.00495708467825	38.0	34.0	38.0	16.0	38.0
40	33.97348229778226	38.0	34.0	38.0	16.0	38.0
41	33.976198524016226	38.0	34.0	38.0	16.0	38.0
42	33.91075543123053	38.0	34.0	38.0	16.0	38.0
43	33.97513160970212	38.0	34.0	38.0	16.0	38.0
44	33.920228697902076	38.0	34.0	38.0	16.0	38.0
45	33.95182567926022	38.0	34.0	38.0	16.0	38.0
46	33.92695039594139	38.0	34.0	38.0	16.0	38.0
47	33.83010808883475	38.0	34.0	38.0	16.0	38.0
48	33.84104844101799	38.0	34.0	38.0	16.0	38.0
49	33.72658456452087	38.0	34.0	38.0	16.0	38.0
50	33.29237274700044	38.0	33.0	38.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	16.0
4	270.0
5	503.0
6	632.0
7	255.0
8	182.0
9	150.0
10	125.0
11	121.0
12	98.0
13	120.0
14	96.0
15	127.0
16	301.0
17	3010.0
18	19521.0
19	57587.0
20	94729.0
21	112349.0
22	117422.0
23	121803.0
24	128408.0
25	138220.0
26	149415.0
27	164749.0
28	185140.0
29	208618.0
30	238401.0
31	275211.0
32	327263.0
33	405373.0
34	537824.0
35	790878.0
36	1450837.0
37	2449331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30072651998532	18.144031784747895	13.115395724724745	25.43984597054204
2	16.543525980735886	21.02630815438111	36.196957420556366	26.23320844432664
3	18.696477158365575	24.920360392877868	29.609641223851902	26.773521224904655
4	13.762830302989288	16.759310296251567	34.96196623069517	34.515893170063976
5	15.40141684645732	35.64074630776231	33.18581097598432	15.772025869796046
6	33.17602878216967	34.498099069523775	17.253493626520537	15.072378521786014
7	28.75813182531947	30.148766274779177	21.926639935608723	19.166461964292633
8	27.96730905677396	30.96984661200205	20.671753144862066	20.391091186361923
9	27.713155770236447	13.599320650828142	19.533282204108417	39.154241374826995
10	17.542078748280822	26.89105322161234	29.67205791061911	25.894810119487733
11	34.8428758315473	22.32626619995451	21.838264629201806	20.992593339296377
12	24.844517784146312	24.708955522362945	28.19824187833224	22.2482848151585
13	30.0661025047041	19.583326176940385	25.099833248731183	25.25073806962433
14	24.997937730751634	19.976067647445984	24.836817460964188	30.1891771608382
15	26.225770226920275	26.184806312279697	23.019738914289498	24.56968454651053
16	25.34193747686901	24.763411962821916	24.985494549360656	24.90915601094842
17	23.687070306339187	25.935963969358305	25.924605350686697	24.452360373615807
18	25.571885642555692	23.099662622795886	27.87561439474399	23.45283733990443
19	25.314529774147903	24.83099176947251	26.6118675317006	23.24261092467898
20	25.899282199137925	22.98553803845565	26.900361828004378	24.214817934402046
21	26.224798710624707	24.026599884461707	26.158680115377823	23.589921289535763
22	25.533612467170098	23.515255930006003	25.531807128105484	25.41932447471842
23	23.49884511355955	24.104594681315668	26.13097807803474	26.26558212709005
24	23.897031261008877	25.656006793941916	25.883775351600786	24.563186593448417
25	25.4518129312794	23.464771342147632	25.31570423087927	25.767711495693696
26	25.242784291765126	23.99026092345972	26.007200508164974	24.75975427661018
27	25.51696565046277	23.475894744865826	25.612109395372723	25.395030209298685
28	23.955897566535768	23.479417075821164	26.96431768274551	25.600367674897562
29	24.313566001632047	23.150859709117196	27.013692887223268	25.521881402027486
30	24.32624038789177	24.005128463574597	27.875446082808114	23.79318506572552
31	26.219631908301285	23.29267830953934	25.72893324316714	24.75875653899224
32	24.14901382746365	23.727449247212377	26.438597905390164	25.684939019933807
33	24.396939391568935	23.975488957218875	26.165165847308554	25.462405803903636
34	24.16017600932699	23.42296771281897	26.239656004563205	26.177200273290836
35	24.589559700093837	24.159634024718876	27.182666070749118	24.068140204438166
36	24.98839397281425	23.53351280311226	26.530870402518207	24.94722282155528
37	25.572492336953655	24.236751056391572	26.322481148423343	23.86827545823143
38	25.36854209758633	23.25824037086527	26.543807181352225	24.82941035019617
39	25.981736602470455	22.997698645415372	25.924252887224597	25.096311864889575
40	25.48614153890129	24.67624099938131	25.334120642609854	24.503496819107546
41	24.79382899293617	24.183493355977177	26.071795239552536	24.950882411534117
42	26.611199086896185	24.432472605443795	25.351318422764606	23.605009884895413
43	25.79882379357601	23.112597900711368	25.244420328620176	25.844157977092447
44	25.10982106187439	24.23972734288693	25.341222564166337	25.309229031072338
45	25.8773405722586	23.587942727768908	25.272070669757245	25.262646030215247
46	24.127215087945547	24.001624246389152	27.132810341035345	24.738350324629955
47	25.39627037435997	23.932443381665944	25.985498337215358	24.685787906758733
48	25.80089070363331	24.689560269128602	24.992878256090766	24.516670771147318
49	24.315705297562033	24.860370597551036	26.740661819486476	24.083262285400455
50	25.79752587439267	24.546475896313428	24.87142703520281	24.784571194091097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	667.0
1	785.5
2	904.0
3	2137.0
4	3370.0
5	3929.5
6	4489.0
7	4297.5
8	4106.0
9	5121.0
10	6136.0
11	8707.0
12	11278.0
13	21706.5
14	32135.0
15	39696.5
16	47258.0
17	45258.5
18	43259.0
19	37384.5
20	31510.0
21	32379.0
22	33248.0
23	36311.0
24	39374.0
25	45419.5
26	51465.0
27	58367.5
28	65270.0
29	77413.0
30	89556.0
31	102708.0
32	115860.0
33	130591.5
34	145323.0
35	169050.5
36	192778.0
37	220347.5
38	247917.0
39	283724.0
40	319531.0
41	336758.5
42	353986.0
43	371945.0
44	389904.0
45	453573.5
46	517243.0
47	627683.5
48	738124.0
49	760737.0
50	783350.0
51	679958.0
52	576566.0
53	512763.5
54	448961.0
55	480969.5
56	512978.0
57	498328.0
58	483678.0
59	462664.0
60	441650.0
61	395779.5
62	349909.0
63	306858.5
64	263808.0
65	224039.0
66	184270.0
67	164920.0
68	145570.0
69	126241.0
70	106912.0
71	87286.0
72	67660.0
73	58163.0
74	48666.0
75	37532.5
76	26399.0
77	20984.0
78	15569.0
79	12711.5
80	9854.0
81	9396.0
82	8938.0
83	8824.0
84	8710.0
85	7034.0
86	5358.0
87	5317.5
88	5277.0
89	2740.0
90	203.0
91	148.5
92	94.0
93	51.0
94	8.0
95	5.5
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03044208703128241
2	0.0
3	1.8799148022611615E-4
4	0.0015415301378541525
5	0.02776007524672315
6	0.02759714929719385
7	0.05128407580568448
8	0.041333060119048734
9	0.040305373360479305
10	0.045393676091932844
11	0.04124533076160988
12	0.042786860899464034
13	0.03175802739286522
14	0.030517283623372856
15	0.04728612365954242
16	0.03475335831113467
17	0.0346280306576506
18	0.03942807978609076
19	0.04123279799626148
20	0.04005471805351115
21	0.031444708259155026
22	0.03438990811603085
23	0.04815088446858255
24	0.03217160864936268
25	0.038939301937502856
26	0.026920379968379832
27	0.03425204769719836
28	0.030993528706612348
29	0.032685452028647394
30	0.030417021500585593
31	0.033876064736746134
32	0.026744921253502123
33	0.02643160211979193
34	0.02801073055369131
35	0.03245986225237606
36	0.03291104180491874
37	0.027145969744651173
38	0.036683404174789463
39	0.036507945459911754
40	0.03195855163843975
41	0.03531733275181302
42	0.03476589107648308
43	0.034640563422999
44	0.030993528706612348
45	0.0
46	0.0
47	0.0
48	0.0
49	0.026018020863294473
50	0.004348869575897487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7979085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.08379127146361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27430883904285	26.557784780918997
2	10.433550702929372	6.903628273518954
3	3.453984918918641	3.4281274833686246
4	1.5646905262364605	2.0706357909777444
5	0.8676521083206086	1.4352610623962174
6	0.5727183823323545	1.1368617251048345
7	0.38151173798059484	0.8835298294874318
8	0.2818721196660935	0.7460318697822439
9	0.23049970849899026	0.686322381970242
>10	1.5131891093709968	9.749382476899221
>50	0.1867555575372627	4.3474249536010054
>100	0.18384583606038007	12.878947788280962
>500	0.02948803073858094	6.7885991379378074
>1k	0.02388410882692588	15.200036640817563
>5k	0.0013526598850116631	2.947052864510667
>10k+	6.956536551488554E-4	4.240372940427706
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	42969	0.5385203942557324	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	37063	0.46450188210803617	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	26022	0.3261276198962663	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	24973	0.3129807490457866	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23691	0.29691374386912783	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	20368	0.2552673646163689	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	17480	0.21907273829016735	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	16618	0.2082694945598399	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15507	0.19434559225775888	No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCAGCTGCTG	14728	0.18458256805134926	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13889	0.17406757792403513	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	12305	0.1542156776121573	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	12025	0.1507065033146031	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	11772	0.14753571368145596	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	11280	0.14136959313003936	No Hit
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCAGCTATGG	10863	0.13614342997975332	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	10073	0.1262425453545112	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	10015	0.12551564496430356	No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCACAGCCAT	9842	0.12334747655902902	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	9601	0.12032708011006274	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG	9253	0.11596567776881685	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	9155	0.11473746676467289	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT	8324	0.10432273876014606	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTTGCAGACA	8102	0.10154046485279954	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	8061	0.10102662147351482	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.77293574388542E-5	0.0	0.0	0.0	0.0
2	8.77293574388542E-5	0.0	0.0	0.0	0.0
3	8.77293574388542E-5	0.0	0.0	1.2532765348407744E-5	0.0
4	8.77293574388542E-5	0.0	0.0	1.2532765348407744E-5	0.0
5	1.127948881356697E-4	0.0	0.0	1.2532765348407744E-5	0.0
6	1.127948881356697E-4	0.0	0.0	1.2532765348407744E-5	0.0
7	1.127948881356697E-4	0.0	0.0	2.5065530696815487E-5	0.0
8	1.127948881356697E-4	0.0	0.0	2.5065530696815487E-5	0.0
9	1.127948881356697E-4	0.0	0.0	6.266382674203871E-5	0.0
10	1.6292594952930066E-4	0.0	0.0	8.77293574388542E-5	0.0
11	1.754587148777084E-4	0.0	0.0	1.3786041883248517E-4	0.0
12	1.754587148777084E-4	0.0	0.0	1.3786041883248517E-4	0.0
13	1.8799148022611615E-4	0.0	0.0	1.5039318418089292E-4	0.0
14	1.8799148022611615E-4	0.0	1.2532765348407744E-5	1.6292594952930066E-4	0.0
15	1.8799148022611615E-4	0.0	1.2532765348407744E-5	1.754587148777084E-4	0.0
16	2.005242455745239E-4	0.0	1.2532765348407744E-5	2.3812254161974713E-4	0.0
17	2.1305701092293164E-4	0.0	1.2532765348407744E-5	2.631880723165626E-4	0.0
18	2.1305701092293164E-4	0.0	1.2532765348407744E-5	3.0078636836178583E-4	0.0
19	2.1305701092293164E-4	1.2532765348407744E-5	1.2532765348407744E-5	3.3838466440700907E-4	0.0
20	2.1305701092293164E-4	1.2532765348407744E-5	1.2532765348407744E-5	4.261140218458633E-4	0.0
21	2.1305701092293164E-4	1.2532765348407744E-5	1.2532765348407744E-5	5.38908909981533E-4	0.0
22	2.1305701092293164E-4	1.2532765348407744E-5	1.2532765348407744E-5	5.514416753299407E-4	0.0
23	2.1305701092293164E-4	1.2532765348407744E-5	1.2532765348407744E-5	7.269003902076491E-4	0.0
24	2.1305701092293164E-4	2.5065530696815487E-5	1.2532765348407744E-5	9.023591050853576E-4	0.0
25	2.255897762713394E-4	2.5065530696815487E-5	1.2532765348407744E-5	9.900884625242117E-4	0.0
26	2.631880723165626E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.0010903505853114737	0.0
27	2.7572083766497034E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.0011906127080987356	0.0
28	2.882536030133781E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.0019676441597000155	0.0
29	2.882536030133781E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.0031081258064051204	0.0
30	3.0078636836178583E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.005075769966105136	0.0
31	3.133191337101936E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.007519659209044646	0.0
32	3.133191337101936E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.009825688033151671	0.0
33	3.258518990586013E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.0131092725544345	0.0
34	3.509174297554168E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.016192332830142805	0.0
35	3.759829604522323E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.021205438969505903	0.0
36	3.759829604522323E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.02676998678419894	0.0
37	3.8851572580064005E-4	2.5065530696815487E-5	1.2532765348407744E-5	0.035029079148799644	0.0
38	4.1358125649745554E-4	3.759829604522323E-5	1.2532765348407744E-5	0.04432839103731819	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAC	3350	0.0	18.980494	3
CTAGACA	3440	0.0	18.612879	4
GGTATAG	3830	0.0	18.216722	1
TAGACAT	3640	0.0	17.89288	5
GTTCTAG	3540	0.0	17.719494	1
CTCTAGT	3355	0.0	17.572304	27
CGTACGA	205	5.456968E-12	17.171667	12
TATAGAG	4095	0.0	16.655676	3
CTCTACC	5805	0.0	16.606634	1
CCTTACC	5730	0.0	16.43123	28
ATCCTTA	5720	0.0	16.383041	26
TCTACCA	5900	0.0	16.37069	2
GTATAGA	4215	0.0	16.338089	2
TAGAGAC	5000	0.0	16.282518	5
AATCTAC	4510	0.0	16.244787	14
TAGACCT	4550	0.0	16.103592	5
GAATCTA	4505	0.0	16.01813	13
CGTCGTA	2875	0.0	15.995117	10
GTAGACC	4435	0.0	15.975016	4
ATACCGT	3040	0.0	15.850442	6
>>END_MODULE