##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2558115_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10634744 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.907312014280738 33.0 32.0 34.0 27.0 34.0 2 31.961353089458477 33.0 32.0 34.0 27.0 34.0 3 31.973594568896065 33.0 32.0 34.0 27.0 34.0 4 31.916067373130936 33.0 32.0 34.0 28.0 34.0 5 31.952284417941794 33.0 33.0 34.0 28.0 34.0 6 35.420792169515316 38.0 36.0 38.0 29.0 38.0 7 35.56095031530613 38.0 37.0 38.0 29.0 38.0 8 35.572781065533874 38.0 37.0 38.0 29.0 38.0 9 35.71467813423624 38.0 37.0 38.0 29.0 38.0 10 35.63020586109078 38.0 37.0 38.0 29.0 38.0 11 35.71730828687555 38.0 37.0 38.0 29.0 38.0 12 35.657477979723815 38.0 37.0 38.0 29.0 38.0 13 35.70617336910038 38.0 37.0 38.0 29.0 38.0 14 35.71541618679302 38.0 37.0 38.0 29.0 38.0 15 35.69027566625017 38.0 37.0 38.0 29.0 38.0 16 35.68401317417702 38.0 37.0 38.0 29.0 38.0 17 35.63528233495794 38.0 37.0 38.0 29.0 38.0 18 35.646095007082444 38.0 37.0 38.0 29.0 38.0 19 35.57694957208185 38.0 37.0 38.0 29.0 38.0 20 35.57435957085568 38.0 37.0 38.0 29.0 38.0 21 35.57916730294589 38.0 37.0 38.0 29.0 38.0 22 35.59913120616726 38.0 37.0 38.0 29.0 38.0 23 35.59005576438887 38.0 37.0 38.0 29.0 38.0 24 35.49188076365543 38.0 37.0 38.0 28.0 38.0 25 35.56060023635736 38.0 37.0 38.0 29.0 38.0 26 35.51545688358836 38.0 37.0 38.0 28.0 38.0 27 35.5111005963096 38.0 37.0 38.0 28.0 38.0 28 35.4954298852892 38.0 37.0 38.0 28.0 38.0 29 35.53873370153527 38.0 37.0 38.0 29.0 38.0 30 35.49466155461758 38.0 37.0 38.0 28.0 38.0 31 35.55551172646939 38.0 37.0 38.0 29.0 38.0 32 35.56135380409721 38.0 37.0 38.0 29.0 38.0 33 35.5144409682076 38.0 37.0 38.0 28.0 38.0 34 35.504737866750716 38.0 37.0 38.0 28.0 38.0 35 35.41322997525845 38.0 37.0 38.0 28.0 38.0 36 35.42720755666521 38.0 37.0 38.0 28.0 38.0 37 35.364443939600235 38.0 37.0 38.0 28.0 38.0 38 35.32928267948904 38.0 37.0 38.0 27.0 38.0 39 35.31238711528928 38.0 37.0 38.0 27.0 38.0 40 35.26778876858719 38.0 37.0 38.0 27.0 38.0 41 35.250300994551445 38.0 37.0 38.0 27.0 38.0 42 35.190414550646445 38.0 37.0 38.0 27.0 38.0 43 35.228593090722256 38.0 37.0 38.0 27.0 38.0 44 35.1647584558688 38.0 37.0 38.0 27.0 38.0 45 35.17176896782847 38.0 37.0 38.0 27.0 38.0 46 35.1210455089469 38.0 37.0 38.0 27.0 38.0 47 34.981796176757996 38.0 36.0 38.0 25.0 38.0 48 34.96766090467246 38.0 36.0 38.0 25.0 38.0 49 34.849163458941746 38.0 36.0 38.0 25.0 38.0 50 34.51455766119053 38.0 35.0 38.0 24.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 22.0 4 266.0 5 631.0 6 1207.0 7 559.0 8 313.0 9 275.0 10 214.0 11 168.0 12 175.0 13 154.0 14 153.0 15 194.0 16 415.0 17 1746.0 18 9974.0 19 30731.0 20 56094.0 21 74623.0 22 86912.0 23 97889.0 24 109380.0 25 122578.0 26 135953.0 27 153147.0 28 173855.0 29 200712.0 30 230641.0 31 264226.0 32 310373.0 33 379653.0 34 496496.0 35 732333.0 36 1504325.0 37 5458357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.74177708235451 17.940885573557235 13.691131425120739 23.626205918967514 2 14.333132983737078 21.973608391513704 37.4390206289874 26.254237995761816 3 18.612958103958512 28.110439181005887 29.35439481907536 23.922207895960245 4 12.717797049019422 18.18837916106814 34.76467485680331 34.329148933109124 5 13.393649963503377 37.88469135895372 33.3991244572538 15.322534220289114 6 31.171929689900125 34.284178609612184 18.914666424355573 15.629225276132114 7 26.101548041214613 32.65117465024881 22.69638426573657 18.550893042800006 8 29.14712025137825 30.762968747060043 20.89641936515702 19.193491636404687 9 28.30501374059935 14.398704956390496 19.476757449986355 37.8195238530238 10 17.385698519761135 28.79247583662709 30.341142699617734 23.48068294399404 11 32.34117876723122 24.083533055956046 21.11579992144374 22.459488255368992 12 24.07010354824725 26.950645991136906 28.887650377524356 20.091600083091485 13 30.376104136429642 20.764344942939683 24.638851285154082 24.220699635476592 14 24.408969369157504 21.41648303839144 25.775916279616673 28.398631312834382 15 27.461253190482033 24.846412772917464 25.130874683209882 22.561459353390624 16 22.731207058079146 26.26556167658542 27.96819175855659 23.035039506778844 17 23.657173830914815 26.574797520120054 27.217702182613447 22.550326466351688 18 25.092214818945862 23.226798029037603 30.247881185316977 21.433105966699557 19 23.579039607628633 27.234452467063576 29.26767931927346 19.91882860603433 20 24.594099030616853 23.50550922720294 30.219635277117142 21.68075646506307 21 24.086742634969664 25.547096516142965 27.852221338189263 22.513939510698105 22 23.703435217479484 25.34253211873594 26.335764259033002 24.618268404751575 23 21.46129483450029 26.51614840807875 27.537790453158017 24.48476630426294 24 22.21698993101685 28.940883397846985 26.460027955235127 22.38209871590104 25 23.775328418412787 24.040272031228408 26.1437999497451 26.040599600613707 26 23.618799185490534 24.70884710489443 27.881993200381068 23.790360509233967 27 24.2852151748412 24.767824749029472 26.14843073574748 24.79852934038184 28 23.067349909577707 24.323911698927866 28.908117311459524 23.700621080034896 29 23.700276582731085 23.556181137701174 29.888234312476214 22.85530796709153 30 23.699307726012492 25.413709760410402 29.595902908935408 21.2910796046417 31 25.793261591992493 23.62613927493169 26.76267654140185 23.81792259167397 32 20.92432413162367 25.245236942526695 29.356870486242965 24.473568439606673 33 22.81216011173097 25.97361307714398 28.1132860093323 23.100940801792753 34 21.031310010539986 25.097702979368254 28.920975510643693 24.950011499448074 35 21.97615380903236 26.839339752484793 29.462351147094783 21.722155291388066 36 24.105804134961822 25.273861285382125 27.665222422370107 22.955112157285946 37 23.851566357356226 26.161898895002732 28.06053239999334 21.9260023476477 38 24.650412772669196 25.09956422600491 27.043049262725187 23.206973738600702 39 24.502703333755722 23.684737088527466 27.41828498395535 24.394274593761462 40 23.700432099659675 26.21033340363474 26.967481429234486 23.1217530674711 41 21.65368808102724 26.354877700830357 27.246102449783884 24.74533176835852 42 25.707540513803874 26.60752324226327 27.152244679405964 20.53269156452689 43 25.179134332146365 23.233837412093205 26.47517424728962 25.111854008470814 44 23.534357542398688 25.465099401471814 28.19178112345172 22.808761932677786 45 26.644317907417424 23.527044938740417 27.15283038312911 22.675806770713052 46 21.94324564841429 24.63593858018585 30.79767599483354 22.62313977656632 47 25.164893484977163 24.734840819863646 28.19562934472141 21.904636350437773 48 24.732997804178456 26.124361808803297 27.241934549623387 21.900705837394863 49 23.237266468437433 25.49557464685127 30.09180879313767 21.175350091573627 50 23.85165619809717 26.459222418844213 25.960550886801965 23.728570496256648 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1320.0 1 1498.0 2 1676.0 3 5128.0 4 8580.0 5 10170.0 6 11760.0 7 10988.0 8 10216.0 9 13145.0 10 16074.0 11 22975.0 12 29876.0 13 55015.0 14 80154.0 15 96560.0 16 112966.0 17 108284.0 18 103602.0 19 87257.5 20 70913.0 21 72712.0 22 74511.0 23 80804.5 24 87098.0 25 99995.0 26 112892.0 27 123544.0 28 134196.0 29 156011.5 30 177827.0 31 197898.0 32 217969.0 33 238713.0 34 259457.0 35 286789.5 36 314122.0 37 342604.5 38 371087.0 39 395884.5 40 420682.0 41 444763.0 42 468844.0 43 488896.0 44 508948.0 45 609566.0 46 710184.0 47 953284.0 48 1196384.0 49 1237631.5 50 1278879.0 51 1050239.5 52 821600.0 53 655302.5 54 489005.0 55 511052.0 56 533099.0 57 508803.0 58 484507.0 59 465232.5 60 445958.0 61 374148.0 62 302338.0 63 262077.5 64 221817.0 65 189615.5 66 157414.0 67 141349.0 68 125284.0 69 106790.0 70 88296.0 71 71041.0 72 53786.0 73 51280.5 74 48775.0 75 38135.5 76 27496.0 77 22743.0 78 17990.0 79 14909.5 80 11829.0 81 10209.0 82 8589.0 83 8292.5 84 7996.0 85 6256.0 86 4516.0 87 4237.5 88 3959.0 89 2070.0 90 181.0 91 132.5 92 84.0 93 44.5 94 5.0 95 3.5 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03130305722450865 2 0.0 3 3.761256500391547E-4 4 0.0023037696064898226 5 0.034076983893547415 6 0.035562680211202076 7 0.06087593645883718 8 0.05025038684523107 9 0.049789632923933104 10 0.05692661713342606 11 0.05095562243905448 12 0.05167966431537985 13 0.03903243933281328 14 0.038731538812781956 15 0.05882605166612379 16 0.04271847070319699 17 0.042803098974455805 18 0.04904678476510577 19 0.05293968524301102 20 0.04987426119519191 21 0.039211099016581874 22 0.042314135629404906 23 0.05892008307863358 24 0.03927692100533873 25 0.046404502073580704 26 0.03347518285348477 27 0.04148665919931876 28 0.03826138175023301 29 0.04033947596669934 30 0.03744330846139785 31 0.04248339217192252 32 0.03286397867217114 33 0.032845172389669185 34 0.035722533612468715 35 0.04106351784302471 36 0.04016081628293074 37 0.034575350379849296 38 0.04628226123731798 39 0.04439222984587123 40 0.04067798905173458 41 0.04269966442069503 42 0.04269026127944406 43 0.04318862776574593 44 0.03918288959282894 45 0.0 46 0.0 47 0.0 48 0.0 49 0.03718002050637044 50 0.006488167463175418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1.0634744E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.898951575191465 #Duplication Level Percentage of deduplicated Percentage of total 1 69.42086776303925 22.144528990809594 2 15.718279054931328 10.027932448372013 3 6.135288048993001 5.871277691240359 4 2.7829494053378645 3.550926732683217 5 1.515158754234055 2.4165987865019773 6 0.9340399161727436 1.7876936433174144 7 0.6141300937327728 1.3713074284594662 8 0.4312988247938669 1.1006384261229238 9 0.3286734346816058 0.9435904179264358 >10 1.7982548162675305 10.586092034869948 >50 0.1614159617126667 3.6093657538457116 >100 0.1288298243052489 8.252280404350234 >500 0.01576688205510293 3.4504110999879005 >1k 0.012713853702249853 7.929806185291016 >5k 0.0010171082742158738 2.192736040274979 >10k+ 0.001316257766632307 14.764813915946922 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 180442 1.6967216136091288 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 131614 1.2375850326063327 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 93253 0.8768711310775322 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 81571 0.7670236349835972 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 78442 0.7376012060092842 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 68017 0.6395734584678296 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 67083 0.6307909245394153 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61155 0.5750491032036126 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 48635 0.4573217747413572 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 46489 0.43714263361675654 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 42220 0.3970006236163278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41705 0.39215800587207367 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 41075 0.38623402688395697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36383 0.3421144881343641 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 36339 0.34170074991932103 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 31525 0.29643402793710877 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 31047 0.2919393264191409 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 24129 0.2268883952448691 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 23380 0.2198454424478859 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 23173 0.2178989922089333 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 22067 0.20749911798535067 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 22010 0.20696313893404486 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 20168 0.1896425527497418 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 19667 0.18493157898300136 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 19508 0.18343647952409572 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 18351 0.17255704509671319 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 16274 0.15302672071843007 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 15645 0.14711214487156438 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 15503 0.14577689881392536 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 15284 0.143717610879961 No Hit GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 15196 0.14289013444987486 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 14862 0.13974948527204792 No Hit GTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCACTTTTTC 14773 0.13891260570071082 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 14748 0.13867752716943632 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 14304 0.1345025324540017 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 12401 0.11660835465338894 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 12383 0.11643909811087132 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 11832 0.11125796728158197 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 11668 0.10971585211642142 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA 10980 0.10324649093574796 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10750 0.10108376844802283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3164397751370415E-4 0.0 0.0 9.403141250978868E-6 0.0 2 1.3164397751370415E-4 0.0 0.0 9.403141250978868E-6 9.403141250978868E-6 3 1.3164397751370415E-4 0.0 0.0 1.8806282501957736E-5 9.403141250978868E-6 4 1.3164397751370415E-4 0.0 0.0 2.82094237529366E-5 9.403141250978868E-6 5 1.5985340126664074E-4 0.0 0.0 4.701570625489433E-5 9.403141250978868E-6 6 1.5985340126664074E-4 0.0 0.0 4.701570625489433E-5 9.403141250978868E-6 7 1.5985340126664074E-4 0.0 0.0 4.701570625489433E-5 9.403141250978868E-6 8 1.5985340126664074E-4 0.0 0.0 5.64188475058732E-5 9.403141250978868E-6 9 1.5985340126664074E-4 0.0 0.0 6.582198875685207E-5 9.403141250978868E-6 10 2.3507853127447168E-4 0.0 0.0 1.128376950117464E-4 9.403141250978868E-6 11 3.103036612823026E-4 0.0 0.0 1.2224083626272527E-4 9.403141250978868E-6 12 3.2910994378426033E-4 0.0 0.0 1.41047118764683E-4 9.403141250978868E-6 13 3.385130850352392E-4 0.0 0.0 1.692565425176196E-4 9.403141250978868E-6 14 3.385130850352392E-4 0.0 0.0 1.8806282501957733E-4 1.8806282501957736E-5 15 3.479162262862181E-4 0.0 0.0 2.1627224877251395E-4 1.8806282501957736E-5 16 3.949319325411124E-4 0.0 0.0 2.538848137764294E-4 1.8806282501957736E-5 17 4.1373821504307013E-4 0.0 0.0 3.385130850352392E-4 1.8806282501957736E-5 18 4.1373821504307013E-4 0.0 0.0 3.7612565003915466E-4 2.82094237529366E-5 19 4.325444975450279E-4 0.0 0.0 4.7015706254894337E-4 2.82094237529366E-5 20 4.4194763879600675E-4 0.0 0.0 6.112041813136264E-4 2.82094237529366E-5 21 5.171727688038376E-4 0.0 0.0 7.80460723831246E-4 2.82094237529366E-5 22 5.171727688038376E-4 0.0 0.0 9.87329831352781E-4 2.82094237529366E-5 23 5.547853338077532E-4 0.0 0.0 0.0013070366338860625 2.82094237529366E-5 24 5.735916163097109E-4 0.0 0.0 0.0018242094026899001 2.82094237529366E-5 25 5.735916163097109E-4 0.0 0.0 0.0019934659452075197 2.82094237529366E-5 26 7.05235593823415E-4 0.0 0.0 0.002341382171493738 2.82094237529366E-5 27 7.992670063332037E-4 9.403141250978868E-6 0.0 0.002811539234042681 2.82094237529366E-5 28 8.368795713371191E-4 9.403141250978868E-6 0.0 0.004861424026756074 2.82094237529366E-5 29 8.838952775920135E-4 9.403141250978868E-6 0.0 0.006948921384473383 2.82094237529366E-5 30 8.932984188429924E-4 9.403141250978868E-6 0.0 0.01062554961360612 2.82094237529366E-5 31 9.309109838469079E-4 9.403141250978868E-6 0.0 0.014640690927774097 2.82094237529366E-5 32 9.685235488508233E-4 9.403141250978868E-6 0.0 0.019229423858251785 2.82094237529366E-5 33 9.685235488508233E-4 9.403141250978868E-6 0.0 0.025435497083897834 2.82094237529366E-5 34 9.87329831352781E-4 9.403141250978868E-6 0.0 0.03254427186963786 4.701570625489433E-5 35 9.87329831352781E-4 9.403141250978868E-6 0.0 0.04253040787817742 4.701570625489433E-5 36 0.0010249423963566964 9.403141250978868E-6 0.0 0.05413388418188534 4.701570625489433E-5 37 0.0010343455376076753 9.403141250978868E-6 0.0 0.0716801457562119 4.701570625489433E-5 38 0.0010437486788586543 9.403141250978868E-6 0.0 0.090768522495699 9.403141250978866E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGAAT 375 0.0 15.838156 35 AATGCGA 790 0.0 15.316762 20 TGCGACG 715 0.0 15.076374 22 CGACGAA 830 0.0 14.841366 38 GTCGACG 760 0.0 14.761023 36 CGTCGTA 2085 0.0 14.669375 10 GTACTAG 1145 0.0 14.415176 1 ACGCGAA 415 0.0 14.3113165 34 TCGACGA 850 0.0 14.233369 37 CCGTCGT 2180 0.0 14.030114 9 TAGGTCG 790 0.0 13.923478 21 CATTGCG 810 0.0 13.849717 29 TACCGTC 2320 0.0 13.65776 7 ATACCGT 2565 0.0 13.211079 6 CAATTCG 850 0.0 13.20101 15 GACGAAC 880 0.0 12.998181 39 CGAGCCG 2580 0.0 12.962231 15 CAATGCG 860 0.0 12.791857 19 AGTCGAC 845 0.0 12.755911 35 ATTCGTA 2935 0.0 12.741035 24 >>END_MODULE