##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558111_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10196119
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.351533657070892	33.0	32.0	34.0	25.0	34.0
2	31.483971303198796	33.0	32.0	34.0	25.0	34.0
3	31.50356346370614	33.0	32.0	34.0	25.0	34.0
4	31.42988376263557	33.0	32.0	34.0	27.0	34.0
5	31.47292160870229	33.0	32.0	34.0	27.0	34.0
6	34.76415065379288	38.0	35.0	38.0	26.0	38.0
7	34.88225873001286	38.0	36.0	38.0	26.0	38.0
8	34.874597677802704	38.0	36.0	38.0	26.0	38.0
9	35.0487723809422	38.0	36.0	38.0	28.0	38.0
10	34.96947956374381	38.0	36.0	38.0	27.0	38.0
11	35.02592986605982	38.0	36.0	38.0	27.0	38.0
12	34.967367093302855	38.0	36.0	38.0	27.0	38.0
13	35.01911040857801	38.0	36.0	38.0	27.0	38.0
14	35.03220029111076	38.0	36.0	38.0	27.0	38.0
15	34.98371694171086	38.0	36.0	38.0	27.0	38.0
16	34.98534716983982	38.0	36.0	38.0	27.0	38.0
17	34.934080898820426	38.0	36.0	38.0	26.0	38.0
18	34.958478613284136	38.0	36.0	38.0	27.0	38.0
19	34.87229709657174	38.0	36.0	38.0	26.0	38.0
20	34.87691963971782	38.0	36.0	38.0	26.0	38.0
21	34.888800140524054	38.0	36.0	38.0	26.0	38.0
22	34.90165346245959	38.0	36.0	38.0	26.0	38.0
23	34.8899310610243	38.0	36.0	38.0	26.0	38.0
24	34.77493652241603	38.0	36.0	38.0	25.0	38.0
25	34.850200747951256	38.0	36.0	38.0	26.0	38.0
26	34.80783619728251	38.0	36.0	38.0	25.0	38.0
27	34.81035539110518	38.0	36.0	38.0	25.0	38.0
28	34.7904358511312	38.0	36.0	38.0	25.0	38.0
29	34.83026100421151	38.0	36.0	38.0	25.0	38.0
30	34.788260513632686	38.0	36.0	38.0	25.0	38.0
31	34.84114740128082	38.0	36.0	38.0	25.0	38.0
32	34.84688919381973	38.0	36.0	38.0	25.0	38.0
33	34.79095398945422	38.0	36.0	38.0	25.0	38.0
34	34.792349716593144	38.0	36.0	38.0	25.0	38.0
35	34.697722535407834	38.0	36.0	38.0	25.0	38.0
36	34.706848360636045	38.0	36.0	38.0	25.0	38.0
37	34.646228530679174	38.0	35.0	38.0	25.0	38.0
38	34.6177893765265	38.0	35.0	38.0	25.0	38.0
39	34.61289869213962	38.0	35.0	38.0	25.0	38.0
40	34.562581801958174	38.0	35.0	38.0	25.0	38.0
41	34.541436109170554	38.0	35.0	38.0	25.0	38.0
42	34.47715282648231	38.0	35.0	38.0	24.0	38.0
43	34.5126386814434	38.0	35.0	38.0	24.0	38.0
44	34.4436690077862	38.0	35.0	38.0	24.0	38.0
45	34.447172203462905	38.0	35.0	38.0	24.0	38.0
46	34.394719010243016	38.0	35.0	38.0	24.0	38.0
47	34.256301049448325	38.0	35.0	38.0	16.0	38.0
48	34.24063871753557	38.0	35.0	38.0	16.0	38.0
49	34.11300603690483	38.0	34.0	38.0	16.0	38.0
50	33.72766147590078	38.0	34.0	38.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	22.0
4	326.0
5	701.0
6	969.0
7	430.0
8	259.0
9	215.0
10	178.0
11	138.0
12	149.0
13	168.0
14	131.0
15	156.0
16	399.0
17	2858.0
18	18001.0
19	53359.0
20	89632.0
21	109348.0
22	117739.0
23	125570.0
24	135467.0
25	147902.0
26	162207.0
27	178816.0
28	202427.0
29	230687.0
30	264411.0
31	303952.0
32	358701.0
33	439772.0
34	577184.0
35	850835.0
36	1665196.0
37	4157814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.806006990732	18.628579983525825	13.83787419586372	23.72753882987845
2	15.077795777001032	21.80584593020148	37.343081225317206	25.773277067480283
3	18.43020474311045	27.648009129770674	29.74202670159769	24.179759425521187
4	12.681669178614971	17.984879663340408	35.52467026816662	33.808780889878
5	13.687171610423073	37.57654820147781	33.557073514920745	15.179206673178374
6	30.931077355776686	35.219007051090806	18.498883192217008	15.351032400915502
7	26.117153927294062	32.516108152262284	22.79962870707809	18.567109213365566
8	28.759277300965415	30.764329204004902	20.825289383497296	19.65110411153239
9	27.807884465675137	14.105109201421492	19.99421284371911	38.09279348918426
10	17.79578972902067	28.006509344426117	30.199193632586287	23.998507293966924
11	32.95944366834911	23.51862578106824	21.559028943324478	21.96290160725817
12	23.785601822423956	26.759414094152422	28.772167764895062	20.68281631852856
13	30.069913946782595	20.555612787011665	25.220283874563037	24.154189391642703
14	24.22396714137132	20.998236646840677	26.28543127685979	28.492364934928215
15	26.63361376779987	25.64762993052022	24.81070305527521	22.908053246404698
16	23.227806787551096	26.046169413173402	27.293245719577175	23.432778079698323
17	22.968738272252978	26.63037084822425	27.26941992263673	23.131470956886044
18	24.388521531866193	23.487865312985665	30.048484193160053	22.075128961988092
19	23.572483112553474	26.432266037162293	29.399680001507168	20.595570848777065
20	24.74084620226492	23.25203220123367	29.728789627862735	22.27833196863868
21	24.750191488708325	24.983617867517776	27.619544435927295	22.646646207846608
22	24.287490018075637	24.829121326727872	27.069173570739036	23.814215084457455
23	22.011254056051648	25.546852468594455	27.89861909515568	24.543274380198216
24	22.51885827534529	27.521431251977564	27.29014297312478	22.669567499552365
25	23.71319395412017	24.067287121205283	26.939760989074607	25.279757935599946
26	23.51013443811582	24.797633517142177	28.026687289935847	23.66554475480616
27	24.1677132951298	24.51146464112785	26.99426260204934	24.326559461693005
28	22.995484621487897	24.21154533121949	29.177863215318794	23.61510683197382
29	23.22027600812803	23.679540629417207	29.941365025502876	23.158818336951885
30	23.468372917284146	25.09099457147564	29.43227373553135	22.008358775708867
31	25.10186196849677	23.841293126995637	27.16613174906674	23.890713155440853
32	21.671596334246278	25.09825570312783	28.827974598763255	24.40217336386264
33	23.04203281860725	25.33862503886223	28.116603661373883	23.502738481156637
34	21.70708697323428	25.057986930792612	28.90585465407296	24.329071441900147
35	22.741504420958446	26.078503898998857	29.237271838906786	21.94271984113591
36	23.584983502802153	25.261605851533364	27.668620062307593	23.484790583356887
37	23.76284482142067	25.849449828954818	28.090877973056006	22.296827376568505
38	24.089747823897653	24.906096501065655	27.399913617219095	23.604242057817594
39	24.231188680069092	23.854312257682178	27.749421478845775	24.165077583402955
40	23.705479971512126	25.72023468058134	27.329962392592837	23.244322955313695
41	21.87208874155928	26.012672740186453	27.669493067826075	24.445745450428188
42	25.11274136630674	26.348145182171383	27.370119679560695	21.16899377196118
43	24.856930956474457	23.533671693203377	26.90095384595158	24.70844350437059
44	23.48287116802533	25.383770192434767	27.945685123355002	23.1876735161849
45	25.69345257739734	24.249059862875278	27.095319307277606	22.96216825244978
46	22.46919636775522	24.854525530743608	29.680587290124798	22.995690811376367
47	24.4606697901427	25.159955469331024	27.878833112873636	22.50054162765264
48	24.52164397061274	26.187620995792614	26.964387135928874	22.32634789766577
49	23.4169617422692	25.692650979096364	29.04754579378444	21.842841484849995
50	23.88935325288121	26.218183901087787	26.009739939381372	23.88272290664963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1745.0
1	1720.5
2	1696.0
3	4416.0
4	7136.0
5	8601.5
6	10067.0
7	9644.0
8	9221.0
9	11978.5
10	14736.0
11	21217.5
12	27699.0
13	53418.5
14	79138.0
15	94646.0
16	110154.0
17	106350.0
18	102546.0
19	86428.5
20	70311.0
21	72175.0
22	74039.0
23	79946.5
24	85854.0
25	101124.5
26	116395.0
27	125721.0
28	135047.0
29	153101.5
30	171156.0
31	192194.5
32	213233.0
33	233518.0
34	253803.0
35	282699.0
36	311595.0
37	341047.0
38	370499.0
39	395141.5
40	419784.0
41	444379.5
42	468975.0
43	492078.5
44	515182.0
45	604465.0
46	693748.0
47	860712.5
48	1027677.0
49	1055358.5
50	1083040.0
51	904553.0
52	726066.0
53	611902.0
54	497738.0
55	517344.0
56	536950.0
57	512881.0
58	488812.0
59	467203.5
60	445595.0
61	376901.5
62	308208.0
63	268742.5
64	229277.0
65	195345.5
66	161414.0
67	146471.0
68	131528.0
69	112985.5
70	94443.0
71	76399.5
72	58356.0
73	55167.0
74	51978.0
75	40564.0
76	29150.0
77	24482.5
78	19815.0
79	16284.5
80	12754.0
81	11316.0
82	9878.0
83	9472.5
84	9067.0
85	7142.0
86	5217.0
87	5150.0
88	5083.0
89	2639.5
90	196.0
91	151.5
92	107.0
93	56.5
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03133545224413328
2	0.0
3	2.451913321137189E-4
4	0.0017849928977878742
5	0.03133545224413328
6	0.031659104802523395
7	0.05670785129126092
8	0.04621366227679374
9	0.04496809030965606
10	0.05130383433147455
11	0.046959043926419454
12	0.04763577200305332
13	0.03519966763824549
14	0.035366397744082824
15	0.05280440528401051
16	0.03883830700681308
17	0.038681384554260304
18	0.04462482244469685
19	0.04728269648480957
20	0.04495828265637151
21	0.035425243663790115
22	0.0387009998608294
23	0.053765555305896294
24	0.035739088568895676
25	0.043653864769526526
26	0.03042334048867025
27	0.0379360029046346
28	0.03548408958349741
29	0.03666100797764326
30	0.03411101812366058
31	0.038200809543317414
32	0.030129110890133783
33	0.030011419050719197
34	0.03241429410543365
35	0.03686696869661878
36	0.037092544722163404
37	0.031364875203986925
38	0.04190810248487685
39	0.041035221342552004
40	0.03640600899224499
41	0.03928945905790233
42	0.03909330599221135
43	0.03934830497760962
44	0.035562550809773795
45	0.0
46	0.0
47	0.0
48	0.0
49	0.031884680828068016
50	0.0056197853320464385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0196119E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.508137396716762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.83495775857305	20.016832538404515
2	14.691274690449022	8.670243041973544
3	6.697891978639782	5.929269503222093
4	3.4011867565406058	4.014507444955745
5	1.946152954391423	2.871367438660418
6	1.216454163639107	2.153717795848257
7	0.8007862350081912	1.6540797173614858
8	0.5675064196333809	1.3396845923248482
9	0.39911618328748316	1.0599457656330218
>10	2.063970319310202	11.190517509444037
>50	0.18419942563947064	3.7892228761858786
>100	0.1528785213232531	9.341438951845028
>500	0.023483078412955327	4.797483996921421
>1k	0.01767864982890385	10.64808863607693
>5k	0.0014169910078919395	2.8850808092043385
>10k+	0.0010458743153488124	9.638519381938393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	110096	1.0797833960156802	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	86574	0.8490877754565243	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	63165	0.6195004197185223	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	57938	0.5682358160001859	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56546	0.554583562628094	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	50731	0.49755205877844305	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	45507	0.4463168780199604	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	43052	0.4222390892063932	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40790	0.4000541774767438	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36471	0.35769492294077776	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	33087	0.32450582422586477	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	31563	0.3095589606202125	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	29003	0.28445136821176764	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	28197	0.27654639966442135	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	24727	0.24251384276703716	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	22635	0.22199623209576114	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	20158	0.19770267490993387	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	17332	0.16998624672779908	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	17259	0.16927028803802702	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	15772	0.15468630760390303	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	14832	0.1454671135164272	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	14474	0.14195597364055873	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	14354	0.14077905524641288	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	13689	0.13425696581218796	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	13172	0.12918640906407625	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	12821	0.12574392276119964	No Hit
GTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCACTTTTTC	11565	0.1134255102358064	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	10961	0.10750168765193895	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	10755	0.10548131107532191	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	10670	0.10464766054613525	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	10272	0.10074421453888484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.198056240810841E-4	0.0	0.0	0.0	0.0
2	5.198056240810841E-4	9.807653284548758E-6	0.0	0.0	0.0
3	5.492285839347305E-4	9.807653284548758E-6	0.0	9.807653284548758E-6	0.0
4	5.492285839347305E-4	9.807653284548758E-6	0.0	9.807653284548758E-6	0.0
5	5.786515437883768E-4	9.807653284548758E-6	0.0	1.9615306569097515E-5	0.0
6	5.786515437883768E-4	9.807653284548758E-6	0.0	1.9615306569097515E-5	0.0
7	5.786515437883768E-4	9.807653284548758E-6	0.0	1.9615306569097515E-5	0.0
8	5.884591970729255E-4	9.807653284548758E-6	0.0	2.9422959853646276E-5	0.0
9	5.884591970729255E-4	9.807653284548758E-6	0.0	6.86535729918413E-5	0.0
10	6.865357299184131E-4	9.807653284548758E-6	0.0	1.863454124064264E-4	0.0
11	7.846122627639007E-4	1.9615306569097515E-5	0.0	2.1576837226007267E-4	0.0
12	7.944199160484494E-4	1.9615306569097515E-5	0.0	2.2557602554462144E-4	0.0
13	7.944199160484494E-4	1.9615306569097515E-5	0.0	2.353836788291702E-4	0.0
14	7.944199160484494E-4	1.9615306569097515E-5	0.0	2.353836788291702E-4	0.0
15	7.944199160484494E-4	1.9615306569097515E-5	0.0	2.9422959853646276E-4	0.0
16	8.630734890402907E-4	1.9615306569097515E-5	0.0	3.4326786495920655E-4	0.0
17	8.728811423248395E-4	1.9615306569097515E-5	0.0	3.726908248128528E-4	0.0
18	9.023041021784858E-4	1.9615306569097515E-5	0.0	4.3153674452014535E-4	0.0
19	9.70957675170327E-4	1.9615306569097515E-5	0.0	4.8057501094288913E-4	0.0
20	0.001010188288308522	1.9615306569097515E-5	0.0	5.099979707965354E-4	0.0
21	0.001137687781007656	1.9615306569097515E-5	0.0	6.276898102111205E-4	0.0
22	0.0011474954342922047	1.9615306569097515E-5	0.0	8.238428759020957E-4	0.0
23	0.0012357643138531434	2.9422959853646276E-5	0.0	0.0010886495145849121	0.0
24	0.001255379620422241	3.923061313819503E-5	0.0	0.0015299939123896062	0.0
25	0.0012848025802758873	3.923061313819503E-5	0.0	0.0016084551386659964	0.0
26	0.0015103786058205087	3.923061313819503E-5	0.0	0.0018438388174951666	0.0
27	0.0015594168722432526	3.923061313819503E-5	0.0	0.0022459526021616654	0.0
28	0.0016084551386659964	3.923061313819503E-5	0.0	0.0036974852882748818	0.0
29	0.0016182627919505452	3.923061313819503E-5	0.0	0.005482478186062756	0.0
30	0.0016476857518041915	3.923061313819503E-5	0.0	0.008493427744419224	0.0
31	0.0017359546313651302	3.923061313819503E-5	0.0	0.012563603857506959	0.0
32	0.001745762284649679	3.923061313819503E-5	0.0	0.016722048850155633	0.0
33	0.001784992897787874	3.923061313819503E-5	0.0	0.021155108134771672	0.0
34	0.001824223510926069	3.923061313819503E-5	0.0	0.026843547039809953	0.0
35	0.0018340311642106178	3.923061313819503E-5	0.0	0.03569985795575748	0.0
36	0.001873261777348813	3.923061313819503E-5	0.0	0.04612539339723281	0.0
37	0.001941915350340654	4.903826642274379E-5	0.0	0.06089571924376324	0.0
38	0.0019811459634788493	4.903826642274379E-5	0.0	0.07584258284941554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGCG	1220	0.0	19.657305	19
CGACGAG	1320	0.0	18.66513	24
TGCGACG	1285	0.0	18.31918	22
GTACTAG	1640	0.0	17.712553	1
AATGCGA	1390	0.0	17.568624	20
CGTCGTA	2995	0.0	16.67677	10
GTCGACG	1300	0.0	16.583252	36
CCGTCGT	3055	0.0	16.493284	9
AATTCGT	1390	0.0	16.144062	16
ATCGTCA	1560	0.0	15.797061	10
TCGACGA	1395	0.0	15.45408	37
TACCGTC	3565	0.0	15.18303	7
AGTCGAC	1460	0.0	15.067329	35
AAGTCGA	1510	0.0	15.004947	34
CGACGAA	1435	0.0	14.870004	38
CAATTCG	1540	0.0	14.857817	15
CATTGCG	1220	0.0	14.784365	29
CGAACAA	1445	0.0	14.765938	41
GACGAGT	1665	0.0	14.666037	25
GGCGTTA	2990	0.0	14.566078	42
>>END_MODULE