FastQCFastQC Report
Wed 25 May 2016
SRR2431463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3797315
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA507561.3366286441867479No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53410.14065201333047164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.043.9630241
TACCTGG39400.043.7889142
GTACCTG48750.036.3590471
GTATCAA93800.031.6280441
ACCTGGG54100.030.760993
ATCAACG105800.027.415353
TCAACGC106350.027.273574
GTCTCGC9250.026.9389061
TATCAAC113200.026.0799942
GTACATG170600.026.0158941
TCTGTCG10750.025.795648
ATAACGC4100.025.2251133
TACATGG170900.025.114412
GTACACG4500.025.0752071
CAACGCA117900.024.6014065
CATGGGT73300.024.2427644
TATAACG3550.023.8362792
TACACGG4400.023.505222
AACGCAG126750.022.9174446
ACATGGG182550.022.533083
Produced by FastQC (version 0.11.5)