##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431463_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3797315
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.592925527642556	31.0	30.0	34.0	24.0	34.0
2	29.622032936430085	31.0	30.0	34.0	23.0	34.0
3	29.729776170794363	31.0	30.0	34.0	23.0	34.0
4	32.92448401041262	37.0	35.0	37.0	23.0	37.0
5	33.133903824149435	37.0	35.0	37.0	26.0	37.0
6	33.11479927264396	37.0	35.0	37.0	25.0	37.0
7	32.99139944934776	37.0	35.0	37.0	25.0	37.0
8	32.89867050797735	37.0	35.0	37.0	23.0	37.0
9	34.1510580502276	39.0	35.0	39.0	18.0	39.0
10-11	34.112773762513775	39.0	35.0	39.0	18.0	39.0
12-13	34.076010549559356	39.0	35.0	39.0	17.5	39.0
14-15	35.22940867428696	40.0	36.0	41.0	18.0	41.0
16-17	35.25230037539683	40.0	36.0	41.0	19.0	41.0
18-19	35.24364096736773	40.0	35.5	41.0	20.0	41.0
20-21	35.347367416187495	40.0	35.5	41.0	22.5	41.0
22-23	35.32413284123124	40.0	35.0	41.0	23.5	41.0
24-25	35.37839196906235	40.0	35.0	41.0	25.0	41.0
26-27	35.38186047246541	40.0	35.0	41.0	25.0	41.0
28-29	35.27471634562842	40.0	35.0	41.0	25.0	41.0
30-31	35.248978686255946	40.0	35.0	41.0	25.0	41.0
32-33	35.185382961381926	40.0	35.0	41.0	25.0	41.0
34-35	35.07119925526326	40.0	35.0	41.0	24.5	41.0
36-37	34.94078210525068	40.0	35.0	41.0	24.0	41.0
38-39	34.825357390682626	40.0	34.0	41.0	24.0	41.0
40-41	34.7350700429119	40.0	34.0	41.0	23.5	41.0
42-43	34.51901475121237	39.0	34.0	41.0	22.0	41.0
44-45	34.40636884219508	39.0	34.0	41.0	22.0	41.0
46-47	34.27609850644468	39.0	33.0	41.0	21.5	41.0
48-49	34.149915532422256	39.0	33.0	41.0	20.0	41.0
50-51	33.99689030275339	39.0	33.0	41.0	19.0	41.0
52-53	33.90043807269083	39.0	33.0	41.0	18.0	41.0
54-55	33.83201169773906	38.5	33.0	41.0	18.0	41.0
56-57	33.67795336968358	38.0	33.0	40.5	16.0	41.0
58-59	33.46978035796346	38.0	33.0	40.0	13.5	41.0
60-61	33.247983114384766	38.0	32.5	40.0	10.5	41.0
62-63	32.960174228369254	37.0	32.0	40.0	7.5	41.0
64-65	32.796726107789326	37.0	32.0	40.0	6.0	41.0
66-67	32.52757474689353	36.5	32.0	40.0	2.0	41.0
68-69	32.24073562504032	36.0	31.5	39.5	2.0	41.0
70-71	31.924861513990805	35.5	31.0	39.0	2.0	41.0
72-73	31.601251542208114	35.0	31.0	39.0	2.0	41.0
74-75	31.264856879136968	35.0	31.0	38.0	2.0	40.0
76-77	30.94040499668845	35.0	31.0	37.0	2.0	39.5
78-79	30.60220971923583	35.0	30.5	37.0	2.0	39.0
80-81	30.3073285992866	35.0	30.0	36.0	2.0	39.0
82-83	30.010594064490306	35.0	30.0	36.0	2.0	38.0
84-85	29.721272530722366	35.0	30.0	35.5	2.0	37.0
86-87	29.464967746947515	35.0	30.0	35.0	2.0	37.0
88-89	29.199191534018116	35.0	30.0	35.0	2.0	36.5
90-91	28.975200108497717	34.0	29.0	35.0	2.0	36.0
92-93	28.777861594310718	34.0	29.0	35.0	2.0	36.0
94-95	28.546005532856768	34.0	29.0	35.0	2.0	36.0
96-97	28.435821626596688	34.0	29.0	35.0	2.0	35.0
98-99	28.26983447514889	34.0	29.0	35.0	2.0	35.0
100	28.132760647984167	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34693623677172525
1101	2	0.41398202186553235
1101	3	0.3863027747133465
1101	4	0.3328476215671543
1101	5	0.3135816532990816
1101	6	0.3243527500783827
1101	7	0.4235163453232218
1101	8	0.46038419360755967
1101	9	0.5221956214799732
1101	10-11	0.5889186061145466
1101	12-13	0.47370351005152145
1101	14-15	0.6370050355045294
1101	16-17	0.5929817511425597
1101	18-19	0.5710885693882517
1101	20-21	0.48806792996944637
1101	22-23	0.3718184170244285
1101	24-25	0.4028326662645654
1101	26-27	0.4979307592266622
1101	28-29	0.5610359077714904
1101	30-31	0.5965057174080997
1101	32-33	0.6211388212831466
1101	34-35	0.5325721073691412
1101	36-37	0.5554073288786867
1101	38-39	0.5354356050086722
1101	40-41	0.5701894883887562
1101	42-43	0.6019126254611891
1101	44-45	0.4963048609972134
1101	46-47	0.4708998804320643
1101	48-49	0.3853114435129754
1101	50-51	0.4145598408462874
1101	52-53	0.40644002466465423
1101	54-55	0.520622265900208
1101	56-57	0.4966210173187733
1101	58-59	0.49631613447049006
1101	60-61	0.4923488623816965
1101	62-63	0.5032180952780294
1101	64-65	0.4458153904136424
1101	66-67	0.5167488366036821
1101	68-69	0.4527828143361248
1101	70-71	0.46480321653602275
1101	72-73	0.4750028557641386
1101	74-75	0.40967726606799815
1101	76-77	0.4446241139879845
1101	78-79	0.35780517031328785
1101	80-81	0.3709810171170922
1101	82-83	0.4416353684368488
1101	84-85	0.3924894646512591
1101	86-87	0.3448544974863026
1101	88-89	0.4432072573483907
1101	90-91	0.37542743410509516
1101	92-93	0.35817992367159235
1101	94-95	0.2910554546847557
1101	96-97	0.36818488761169377
1101	98-99	0.3850032731469035
1101	100	0.3544350225705628
1102	1	0.34252789433045194
1102	2	0.39677738612397917
1102	3	0.4386182467037969
1102	4	0.4169193872490524
1102	5	0.4010717047209411
1102	6	0.36754607305265097
1102	7	0.3824239962271463
1102	8	0.49341765406584415
1102	9	0.496163139598238
1102	10-11	0.5570255958751744
1102	12-13	0.5565251085803098
1102	14-15	0.6597338737069052
1102	16-17	0.5971045956999745
1102	18-19	0.5382986369418532
1102	20-21	0.4930055817669796
1102	22-23	0.6041795197793576
1102	24-25	0.5460537471094185
1102	26-27	0.5969637655812079
1102	28-29	0.6516992464700238
1102	30-31	0.6663804342399118
1102	32-33	0.703065375530791
1102	34-35	0.7523015365158159
1102	36-37	0.6381158975285359
1102	38-39	0.6261570176794891
1102	40-41	0.7077092393857072
1102	42-43	0.6774397220538262
1102	44-45	0.6507027898079514
1102	46-47	0.6064026150110564
1102	48-49	0.6067741451056676
1102	50-51	0.4983997535461171
1102	52-53	0.5253857421893073
1102	54-55	0.5824744656210967
1102	56-57	0.5929596429307153
1102	58-59	0.5405085140173185
1102	60-61	0.6169220505747504
1102	62-63	0.6073346470120953
1102	64-65	0.6886290286626107
1102	66-67	0.719120348737242
1102	68-69	0.7549478219438512
1102	70-71	0.6790090810080294
1102	72-73	0.6125396143917712
1102	74-75	0.5993279031136076
1102	76-77	0.5232324972050577
1102	78-79	0.5054456308430773
1102	80-81	0.5427202450684483
1102	82-83	0.566638265048951
1102	84-85	0.47383510201327894
1102	86-87	0.46930250243098826
1102	88-89	0.4428505045784661
1102	90-91	0.44982935191387696
1102	92-93	0.4790488853337749
1102	94-95	0.4795272148958709
1102	96-97	0.49365211025780553
1102	98-99	0.5040633492716431
1102	100	0.4900948651380155
1103	1	0.2458354199897741
1103	2	0.2916843525302788
1103	3	0.35689578486615403
1103	4	0.32487989390814676
1103	5	0.19810054289672507
1103	6	0.28692733432853856
1103	7	0.38574026672155526
1103	8	0.29539908794812675
1103	9	0.32626879594496216
1103	10-11	0.4011770189409276
1103	12-13	0.33180106538498677
1103	14-15	0.4520032312842517
1103	16-17	0.2966473657514115
1103	18-19	0.29667556431912345
1103	20-21	0.2983247318106521
1103	22-23	0.3174199288294304
1103	24-25	0.34438098508449144
1103	26-27	0.3812618416133944
1103	28-29	0.44340091850330765
1103	30-31	0.36546748386788863
1103	32-33	0.3970080156589688
1103	34-35	0.40138749303941523
1103	36-37	0.42245655257914905
1103	38-39	0.46215825444901526
1103	40-41	0.47896163510615253
1103	42-43	0.44646983655911754
1103	44-45	0.4443182794094014
1103	46-47	0.4294272973080737
1103	48-49	0.3991840967279927
1103	50-51	0.33605485725049533
1103	52-53	0.4001545506118305
1103	54-55	0.5218618795130965
1103	56-57	0.4720163354883411
1103	58-59	0.47674065607508
1103	60-61	0.5889115630189607
1103	62-63	0.43627999908019177
1103	64-65	0.43850680918182405
1103	66-67	0.47125429670755636
1103	68-69	0.4668955569795088
1103	70-71	0.4439420320671381
1103	72-73	0.32678338916229777
1103	74-75	0.4272261652858447
1103	76-77	0.4202968872996564
1103	78-79	0.3699075059647505
1103	80-81	0.23651677357713297
1103	82-83	0.3258188323597153
1103	84-85	0.31950193212507827
1103	86-87	0.3001633377501278
1103	88-89	0.23944111200096074
1103	90-91	0.31837386780917853
1103	92-93	0.30617796638694017
1103	94-95	0.29658703477309345
1103	96-97	0.3606809588441102
1103	98-99	0.24284013423302397
1103	100	0.2893246950197401
1104	1	0.283843879156116
1104	2	0.4121168759822993
1104	3	0.27831238801434566
1104	4	0.33872791786083667
1104	5	0.28967309428026766
1104	6	0.3483625695627808
1104	7	0.3819309848871768
1104	8	0.39096790627200306
1104	9	0.5079656774784382
1104	10-11	0.4452536927980475
1104	12-13	0.31683378777852766
1104	14-15	0.42602575252765007
1104	16-17	0.5045442482812632
1104	18-19	0.4419250466227922
1104	20-21	0.4097527433751651
1104	22-23	0.3908467879280195
1104	24-25	0.44579748859585067
1104	26-27	0.501488864116368
1104	28-29	0.5492869638679565
1104	30-31	0.4924020573475545
1104	32-33	0.4786514254303498
1104	34-35	0.4668187461219233
1104	36-37	0.5334120365069026
1104	38-39	0.5412051285927646
1104	40-41	0.538579826797438
1104	42-43	0.4385932965474595
1104	44-45	0.4616956887523429
1104	46-47	0.4869068676076651
1104	48-49	0.4577078609407721
1104	50-51	0.41637932456712434
1104	52-53	0.3053071859227927
1104	54-55	0.3762224851057141
1104	56-57	0.4429103434958179
1104	58-59	0.4904373981169954
1104	60-61	0.4906938167042725
1104	62-63	0.5315390299128069
1104	64-65	0.5785980931185435
1104	66-67	0.5777335963271142
1104	68-69	0.554957404877257
1104	70-71	0.47519835277815403
1104	72-73	0.4309907201076726
1104	74-75	0.4843221338138939
1104	76-77	0.46572986742044975
1104	78-79	0.531912248677564
1104	80-81	0.5039040635717704
1104	82-83	0.4713490414143777
1104	84-85	0.4811082936041551
1104	86-87	0.49202815715008086
1104	88-89	0.5158898729340251
1104	90-91	0.5207316071824941
1104	92-93	0.49879786684975613
1104	94-95	0.5629981329867704
1104	96-97	0.6226404498327902
1104	98-99	0.6403624985484235
1104	100	0.5714377263538601
1105	1	0.15129572101590583
1105	2	0.25279283558826293
1105	3	0.26501775826628915
1105	4	0.2725561494977171
1105	5	0.14267460490489725
1105	6	0.20638107613075363
1105	7	0.28490087646954976
1105	8	0.2733267143401221
1105	9	0.38030920449317307
1105	10-11	0.40832135657731783
1105	12-13	0.3483602793888352
1105	14-15	0.4832077424118211
1105	16-17	0.48021231486139726
1105	18-19	0.4194769995336429
1105	20-21	0.378268124537982
1105	22-23	0.35562230082093294
1105	24-25	0.41742459787408137
1105	26-27	0.4091553529006262
1105	28-29	0.497948888406647
1105	30-31	0.5149265016107236
1105	32-33	0.4790571235588885
1105	34-35	0.5408482066202964
1105	36-37	0.5178933102161238
1105	38-39	0.49252654882270264
1105	40-41	0.43290743374173957
1105	42-43	0.41731148792852935
1105	44-45	0.3697628418683081
1105	46-47	0.39522239246839064
1105	48-49	0.32033947813783925
1105	50-51	0.41957086506920405
1105	52-53	0.35283506563090583
1105	54-55	0.5256049709564579
1105	56-57	0.4420187949493837
1105	58-59	0.47348970476943464
1105	60-61	0.4266464886549741
1105	62-63	0.48328084501578417
1105	64-65	0.48107378238857734
1105	66-67	0.5055894141418662
1105	68-69	0.4935182310285242
1105	70-71	0.46423204020915065
1105	72-73	0.5025788215067877
1105	74-75	0.5430561193395427
1105	76-77	0.4583527329327737
1105	78-79	0.5015599266000237
1105	80-81	0.433508049432465
1105	82-83	0.4622818298675284
1105	84-85	0.4349181413617451
1105	86-87	0.44838206317411533
1105	88-89	0.4632298026453867
1105	90-91	0.45340760110244105
1105	92-93	0.45854137952383667
1105	94-95	0.49702358910784383
1105	96-97	0.4734344544026996
1105	98-99	0.46019768135699124
1105	100	0.4470977323847052
1106	1	0.21825517806325578
1106	2	0.31459257333534296
1106	3	0.22985317890693224
1106	4	0.19397352470975449
1106	5	0.227767868964726
1106	6	0.3461130654892983
1106	7	0.25129890077185024
1106	8	0.36920284792088154
1106	9	0.38758271511144216
1106	10-11	0.4202068839907511
1106	12-13	0.3828317061252946
1106	14-15	0.36839378370923725
1106	16-17	0.36552465364371756
1106	18-19	0.39276001858640086
1106	20-21	0.4131169366367402
1106	22-23	0.4224964815552852
1106	24-25	0.40892874110663513
1106	26-27	0.45364183266460145
1106	28-29	0.5877904826316254
1106	30-31	0.5449255216866646
1106	32-33	0.5405433860817723
1106	34-35	0.48041461675785513
1106	36-37	0.45775892529648843
1106	38-39	0.44111632947387136
1106	40-41	0.5098493529429433
1106	42-43	0.49283446391360286
1106	44-45	0.436419137855232
1106	46-47	0.42491117128928835
1106	48-49	0.4122292423648446
1106	50-51	0.5192249025839359
1106	52-53	0.48299441208279603
1106	54-55	0.5968605990439713
1106	56-57	0.5735309452383959
1106	58-59	0.5669737529967662
1106	60-61	0.5815906136743152
1106	62-63	0.6138201357473179
1106	64-65	0.6199661799770126
1106	66-67	0.5955924713416394
1106	68-69	0.6338331354787101
1106	70-71	0.573030376705649
1106	72-73	0.6076674186037465
1106	74-75	0.5716837795028873
1106	76-77	0.5887445012944283
1106	78-79	0.5448765572675924
1106	80-81	0.5455860545268756
1106	82-83	0.45072693655350093
1106	84-85	0.4843714482945849
1106	86-87	0.500247832605865
1106	88-89	0.5406506318620821
1106	90-91	0.5322716126050757
1106	92-93	0.4922031679521126
1106	94-95	0.5818447601896146
1106	96-97	0.5905366346724925
1106	98-99	0.5538318422693109
1106	100	0.5591392218855944
1107	1	0.17934444118072435
1107	2	0.17385993358093543
1107	3	0.250918624287646
1107	4	0.21667424126165002
1107	5	0.30370653329624986
1107	6	0.2127789039911434
1107	7	0.3447937076165317
1107	8	0.3477370891293958
1107	9	0.4891103663654448
1107	10-11	0.45208908323487407
1107	12-13	0.41606104504050023
1107	14-15	0.5039440960432913
1107	16-17	0.43330201183823647
1107	18-19	0.44246983236686077
1107	20-21	0.47798859047753695
1107	22-23	0.4806298553532784
1107	24-25	0.5003676591572841
1107	26-27	0.4916550383297462
1107	28-29	0.5334459021417004
1107	30-31	0.5033648801124357
1107	32-33	0.5233418155532448
1107	34-35	0.4726122107284638
1107	36-37	0.48761527443879515
1107	38-39	0.4891173547378713
1107	40-41	0.47806589564861923
1107	42-43	0.4760126899742332
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2216	94-95	-1.4995546577385106
2216	96-97	-1.67253594790974
2216	98-99	-1.9231035847718836
2216	100	-1.8478579352488396
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	142482.0
3	28649.0
4	20437.0
5	18129.0
6	12183.0
7	11264.0
8	12188.0
9	12371.0
10	12286.0
11	12769.0
12	14206.0
13	15674.0
14	16587.0
15	17317.0
16	17092.0
17	16680.0
18	15503.0
19	14250.0
20	13709.0
21	14846.0
22	18379.0
23	28635.0
24	44796.0
25	53676.0
26	56644.0
27	44970.0
28	46973.0
29	56491.0
30	64342.0
31	73451.0
32	91697.0
33	119853.0
34	162077.0
35	249778.0
36	382588.0
37	662395.0
38	849510.0
39	351437.0
40	1001.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62566926047902	21.855871687410843	13.002037543906228	21.516421508203905
2	16.126389233096187	23.608673128487485	37.90223201716948	22.362705621246842
3	17.378684169426165	29.626112128172366	29.523027680307578	23.472176022093887
4	12.269532124468324	20.936936225128065	37.52169504470694	29.271836605696677
5	11.760727359497606	40.29769885987278	32.863629048973934	15.077944731655682
6	26.716652514427906	40.97544569016922	18.504596492632757	13.803305302770116
7	24.45536275130798	36.27837029565025	21.86458109739958	17.401685855642192
8	23.451011975573213	37.558569589139154	20.865061408029913	18.12535702725772
9	24.002557783872003	21.115885886811217	21.20448128369568	33.677075045621095
10-11	21.772419360934258	30.814247397031146	27.745872232413593	19.667461009621
12-13	22.769439029031226	29.17825960141242	27.19089183461032	20.86140953494604
14-15	20.965378855755592	30.937169639287582	25.129102398586667	22.96834910637016
16-17	19.856782921084836	33.61083436504092	25.60653477137702	20.925847942497214
18-19	19.811612563744898	32.123900319417466	27.668673873015457	20.395813243822175
20-21	21.972337749348974	31.384616316503593	26.67947121583698	19.963574718310454
22-23	21.36477869405626	31.74115516929205	26.752206475400392	20.141859661251296
24-25	20.854548645992622	31.475089874971047	27.096647016042986	20.573714462993344
26-27	20.614719888207848	31.226243871387933	27.252444762001204	20.906591478403016
28-29	20.72303192560442	31.246461494009086	27.29146042529632	20.739046155090175
30-31	21.137889568884738	31.96893035230738	26.56994239260569	20.323237686202194
32-33	20.3397097438581	31.48864217100733	27.385162523608514	20.786485561526057
34-35	20.59536417815206	30.722054133616812	27.4504164949898	21.232165193241322
36-37	21.279177838813997	31.375247990725352	26.7354741893439	20.610099981116754
38-39	20.992913890462834	31.27374441889701	27.148570749101314	20.58477094153884
40-41	20.606825786033582	31.009301473643013	27.3888150823006	20.9950576580228
42-43	20.471916092686133	31.08514944782122	27.40814061909705	21.034793840395597
44-45	20.988417728661275	31.22902121452214	27.105430285086975	20.677130771729612
46-47	20.767750674009555	31.74027331196062	26.822814956757252	20.669161057272575
48-49	20.463176024665852	31.07878864491221	27.36093691259437	21.09709841782757
50-51	20.53026271902744	30.815948938619975	27.39313678357391	21.26065155877867
52-53	20.581728536427065	30.951914754712273	27.166214116331222	21.300142592529436
54-55	20.828893553254975	31.525080916802516	26.931087063582567	20.714938466359943
56-57	20.421973730765163	31.177022313535613	26.891723185590777	21.509280770108443
58-59	20.37322188293758	30.600086115972143	27.444093747901416	21.58259825318886
60-61	20.92736903605496	31.25704555889972	26.764439700550003	21.05114570449532
62-63	20.655933148116475	31.191751858390347	26.949632627768494	21.20268236572469
64-65	20.614538182510575	30.843576755647025	27.263829360818086	21.278055701024318
66-67	20.521397732737313	30.921621267162084	26.862053872639102	21.6949271274615
68-69	21.133506533180764	31.28621296734002	26.759731957215337	20.82054854226388
70-71	21.171555059269075	30.911658664924985	26.914966461158414	21.001819814647533
72-73	20.753596486654036	30.976306615070452	27.417453171802848	20.852643726472657
74-75	20.73721426314126	30.99633940798483	27.13908932897925	21.12735699989466
76-77	20.907621644269895	31.0388789210152	26.69154289780441	21.361956536910494
78-79	21.131492764956928	31.099314925542743	26.936236998076335	20.832955311423994
80-81	20.776974555971556	30.894501214532998	27.14341338879264	21.18511084070281
82-83	20.624403185259197	30.97650873976428	27.227498273747102	21.171589801229416
84-85	20.81400882280055	30.741489731794974	27.04206012471798	21.402441320686492
86-87	21.225570072783654	30.86964450896546	26.830484203873233	21.074301214377652
88-89	20.839999357428198	30.779307923832068	27.0335751668119	21.347117551927834
90-91	20.700080256346276	30.573243627060148	26.986147056751577	21.740529059842004
92-93	20.950233763552372	30.89796384874907	26.72122949120842	21.430572896490137
94-95	20.878630499686746	30.587092358421476	26.98959691502168	21.544680226870103
96-97	20.892095712962764	30.715719364766525	26.935733351205922	21.456451571064793
98-99	20.759173316812486	30.738691240768745	26.931355747523355	21.570779694895414
100	20.98649817009372	30.992083880997235	26.565335567968834	21.456082380940217
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	42717.0
1	31821.5
2	18143.5
3	12875.0
4	8727.5
5	6100.0
6	4785.5
7	4335.0
8	4311.0
9	4280.5
10	3763.5
11	3144.0
12	2890.5
13	2850.0
14	2937.5
15	3075.5
16	3330.5
17	3668.5
18	4179.5
19	5025.0
20	6103.5
21	7668.0
22	10098.5
23	14042.5
24	19037.5
25	24742.5
26	32722.0
27	43111.5
28	55686.0
29	66687.0
30	74094.0
31	82018.5
32	90741.0
33	97658.5
34	104307.5
35	110921.5
36	115194.0
37	118300.0
38	120560.5
39	121120.5
40	121871.0
41	121134.5
42	117984.0
43	116309.5
44	114852.0
45	113155.5
46	112980.0
47	112893.5
48	113179.5
49	114742.0
50	115685.0
51	111305.0
52	104277.5
53	98385.5
54	93949.5
55	91327.5
56	84439.5
57	75402.0
58	69222.0
59	63616.0
60	55379.0
61	46024.5
62	37741.5
63	29760.0
64	23024.0
65	17708.5
66	13511.0
67	10090.0
68	7355.0
69	5417.0
70	4104.5
71	3179.5
72	2419.5
73	1889.0
74	1508.5
75	1165.0
76	864.0
77	669.5
78	521.5
79	374.0
80	265.0
81	191.5
82	145.0
83	120.0
84	115.0
85	96.0
86	73.5
87	64.5
88	52.5
89	42.0
90	38.0
91	36.5
92	29.0
93	25.0
94	23.5
95	20.0
96	17.5
97	18.0
98	19.0
99	28.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015458290923981812
2	0.009111701294203932
3	0.011271121832136655
4	0.009612054833481026
5	0.012061153736258382
6	0.013351539179657207
7	0.011323790625744769
8	0.008848357326163354
9	0.007320962311528014
10-11	0.006754772780240776
12-13	0.007439467097146274
14-15	0.008664016548534952
16-17	0.005582892122460212
18-19	0.006491428812200199
20-21	0.0059779080745210765
22-23	0.006649435193024545
24-25	0.006175416050551508
26-27	0.007070785541889467
28-29	0.018210235390005835
30-31	0.006135914455345421
32-33	0.005806734495294702
34-35	0.005543390527254126
36-37	0.007847650247609165
38-39	0.006280753637767739
40-41	0.006017409669727162
42-43	0.005806734495294702
44-45	0.0046216866391121094
46-47	0.006899611962663092
48-49	0.0042793394806593605
50-51	0.004200336290247188
52-53	0.003462973179733575
54-55	0.005398551344831809
56-57	0.0035683107669498056
58-59	0.002896783648446336
60-61	0.0040423299094228425
62-63	0.003805320338186324
64-65	0.005359049749625722
66-67	0.0036078123621558914
68-69	0.004213503488649217
70-71	0.00592523928091296
72-73	0.0036078123621558914
74-75	0.0030284556324666244
76-77	0.003054790029270682
78-79	0.003594645163753863
80-81	0.004648021035916167
82-83	0.001922410966696205
84-85	0.002844114854838221
86-87	0.0026992756724159043
88-89	0.0019487453635002627
90-91	0.002830947656436192
92-93	0.002686108474013876
94-95	0.002001414157108378
96-97	0.001724902990665773
98-99	0.002435931704375328
100	0.0033181339973112583
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3797315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.04195736787072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82341395232308	51.26829129877023
2	13.896803045341175	18.077505424495534
3	3.9050254404262996	7.619714946499739
4	1.3517630224429718	3.5168525150879932
5	0.6287918081205088	2.0448924988520236
6	0.329472472858735	1.285772072013887
7	0.22745236189759874	1.0355762778035582
8	0.13670231110680886	0.7113108712878768
9	0.1019085578515113	0.5965498867679266
>10	0.5286137987279207	6.306364299878029
>50	0.04289230849306986	1.9241652661340272
>100	0.025132186232214547	3.006645768634438
>500	0.0015418379882576093	0.686154778720713
>1k	4.0574682480679807E-4	0.43977241984005744
>5k	4.0574682480678906E-5	0.14095202198546056
>10k+	4.0574682480678906E-5	1.3394796532286157
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50756	1.3366286441867479	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5341	0.14065201333047164	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.6334396804057605E-5	0.0	0.0	0.0	0.0
6	2.6334396804057605E-5	0.0	0.0	0.0	0.0
7	2.6334396804057605E-5	0.0	0.0	0.0	0.0
8	2.6334396804057605E-5	0.0	0.0	2.6334396804057605E-5	0.0
9	2.6334396804057605E-5	0.0	0.0	2.6334396804057605E-5	0.0
10-11	2.6334396804057605E-5	0.0	0.0	2.6334396804057605E-5	0.0
12-13	2.6334396804057605E-5	0.0	0.0	2.6334396804057605E-5	0.0
14-15	2.6334396804057605E-5	0.0	0.0	2.6334396804057605E-5	0.0
16-17	2.6334396804057605E-5	0.0	0.0	3.9501595206086404E-5	0.0
18-19	2.6334396804057605E-5	0.0	0.0	5.266879360811521E-5	0.0
20-21	2.6334396804057605E-5	0.0	0.0	2.765111664426048E-4	0.0
22-23	2.6334396804057605E-5	0.0	0.0	0.0011192118641724482	0.0
24-25	2.6334396804057605E-5	0.0	0.0	0.002725610069219962	0.0
26-27	2.6334396804057605E-5	0.0	0.0	0.004674355432720225	0.0
28-29	2.6334396804057605E-5	0.0	0.0	0.009980736388737832	0.0
30-31	2.6334396804057605E-5	0.0	0.0	0.019829800793455376	0.0
32-33	2.6334396804057605E-5	0.0	0.0	0.038764232095572795	0.0
34-35	2.6334396804057605E-5	0.0	0.0	0.06907512281704309	0.0
36-37	2.6334396804057605E-5	0.0	0.0	0.11383043018553898	0.0
38-39	2.6334396804057605E-5	0.0	0.0	0.17562409228626014	0.0
40-41	2.6334396804057605E-5	0.0	0.0	0.24693763883164815	0.0
42-43	2.6334396804057605E-5	0.0	0.0	0.3203184355261547	0.0
44-45	2.6334396804057605E-5	0.0	0.0	0.41371337379174494	0.0
46-47	2.6334396804057605E-5	0.0	0.0	0.5240939979959525	0.0
48-49	2.6334396804057605E-5	0.0	0.0	0.6440208410416308	0.0
50-51	2.6334396804057605E-5	0.0	0.0	0.7709921352323945	2.6334396804057605E-5
52-53	2.6334396804057605E-5	0.0	0.0	0.8974894102806852	2.6334396804057605E-5
54-55	2.6334396804057605E-5	0.0	0.0	1.041867740758931	2.6334396804057605E-5
56-57	2.6334396804057605E-5	0.0	0.0	1.2125146320492242	2.6334396804057605E-5
58-59	2.6334396804057605E-5	0.0	0.0	1.4065728020983248	2.6334396804057605E-5
60-61	2.6334396804057605E-5	0.0	0.0	1.6071619025548314	2.6334396804057605E-5
62-63	2.6334396804057605E-5	0.0	0.0	1.8128862103881296	2.6334396804057605E-5
64-65	2.6334396804057605E-5	0.0	0.0	2.0395331964822514	2.6334396804057605E-5
66-67	2.6334396804057605E-5	0.0	0.0	2.3092369213510073	2.6334396804057605E-5
68-69	2.6334396804057605E-5	0.0	0.0	2.6060519077295403	2.6334396804057605E-5
70-71	2.6334396804057605E-5	0.0	0.0	2.9309788626964055	2.6334396804057605E-5
72-73	2.6334396804057605E-5	0.0	0.0	3.28615087239273	2.6334396804057605E-5
74-75	2.6334396804057605E-5	0.0	0.0	3.645417880792086	2.6334396804057605E-5
76-77	2.6334396804057605E-5	0.0	0.0	4.02317163574789	2.6334396804057605E-5
78-79	2.6334396804057605E-5	1.3167198402028802E-5	0.0	4.450565728679344	2.6334396804057605E-5
80-81	2.6334396804057605E-5	2.6334396804057605E-5	0.0	4.912536884614523	2.6334396804057605E-5
82-83	2.6334396804057605E-5	2.6334396804057605E-5	0.0	5.400078739846444	2.6334396804057605E-5
84-85	2.6334396804057605E-5	2.6334396804057605E-5	0.0	5.8832622524072935	2.6334396804057605E-5
86-87	2.6334396804057605E-5	2.6334396804057605E-5	0.0	6.3973886812129095	2.6334396804057605E-5
88	2.6334396804057605E-5	2.6334396804057605E-5	0.0	6.809759000767648	2.6334396804057605E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5775	0.0	43.963024	1
TACCTGG	3940	0.0	43.788914	2
GTACCTG	4875	0.0	36.359047	1
GTATCAA	9380	0.0	31.628044	1
ACCTGGG	5410	0.0	30.76099	3
ATCAACG	10580	0.0	27.41535	3
TCAACGC	10635	0.0	27.27357	4
GTCTCGC	925	0.0	26.938906	1
TATCAAC	11320	0.0	26.079994	2
GTACATG	17060	0.0	26.015894	1
TCTGTCG	1075	0.0	25.79564	8
ATAACGC	410	0.0	25.225113	3
TACATGG	17090	0.0	25.11441	2
GTACACG	450	0.0	25.075207	1
CAACGCA	11790	0.0	24.601406	5
CATGGGT	7330	0.0	24.242764	4
TATAACG	355	0.0	23.836279	2
TACACGG	440	0.0	23.50522	2
AACGCAG	12675	0.0	22.917444	6
ACATGGG	18255	0.0	22.53308	3
>>END_MODULE