##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431463_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3797315
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25222216223832	33.0	31.0	34.0	30.0	34.0
2	32.355351610282526	34.0	31.0	34.0	30.0	34.0
3	32.45278255820231	34.0	31.0	34.0	30.0	34.0
4	36.01885621814361	37.0	35.0	37.0	35.0	37.0
5	35.9444586503885	37.0	35.0	37.0	35.0	37.0
6	35.99232668345923	37.0	35.0	37.0	35.0	37.0
7	35.934954566581915	37.0	35.0	37.0	35.0	37.0
8	35.91954841776361	37.0	35.0	37.0	35.0	37.0
9	37.599936270759734	39.0	37.0	39.0	35.0	39.0
10-11	37.53535392770945	39.0	37.0	39.0	34.5	39.0
12-13	37.51649178432656	39.0	37.0	39.0	35.0	39.0
14-15	38.82495368437962	40.0	38.0	41.0	34.5	41.0
16-17	38.876672332951046	40.0	38.0	41.0	35.0	41.0
18-19	38.976638888267104	40.5	38.5	41.0	35.0	41.0
20-21	38.97464129259753	40.5	38.5	41.0	35.0	41.0
22-23	38.86176048076075	40.0	38.0	41.0	35.0	41.0
24-25	38.78060050325033	40.0	38.0	41.0	35.0	41.0
26-27	38.603115359141924	40.0	38.0	41.0	34.0	41.0
28-29	38.47781234898869	40.0	38.0	41.0	34.0	41.0
30-31	38.22596189676126	40.0	37.5	41.0	34.0	41.0
32-33	37.92074715950612	40.0	37.0	41.0	33.5	41.0
34-35	37.6912689624116	40.0	36.5	41.0	33.0	41.0
36-37	37.49454087954252	40.0	36.0	41.0	33.0	41.0
38-39	37.35630544213477	40.0	36.0	41.0	33.0	41.0
40-41	37.19954494162323	40.0	36.0	41.0	32.0	41.0
42-43	37.02980540197481	40.0	36.0	41.0	31.5	41.0
44-45	36.75208469668701	40.0	35.5	41.0	31.0	41.0
46-47	36.33856856752732	40.0	35.0	41.0	30.0	41.0
48-49	35.998053493060226	40.0	35.0	41.0	28.5	41.0
50-51	35.83359057649945	40.0	35.0	41.0	27.5	41.0
52-53	35.68379749902233	40.0	35.0	41.0	27.0	41.0
54-55	35.52358021391431	39.0	35.0	41.0	27.0	41.0
56-57	35.300887995860236	39.0	35.0	41.0	26.0	41.0
58-59	35.12806930686551	39.0	35.0	41.0	26.0	41.0
60-61	34.906763463131185	39.0	35.0	41.0	26.0	41.0
62-63	34.664684388837905	38.0	35.0	41.0	26.0	41.0
64-65	34.41371060868008	37.5	35.0	40.5	25.0	41.0
66-67	34.14504090390183	37.0	34.0	40.0	24.5	41.0
68-69	33.84354432013146	36.5	34.0	40.0	23.5	41.0
70-71	33.50992345907569	36.0	34.0	39.0	23.5	41.0
72-73	33.18034045108189	35.5	34.0	39.0	22.5	41.0
74-75	32.87566543728924	35.0	34.0	39.0	22.0	40.5
76-77	32.53418770894697	35.0	33.0	37.5	21.0	40.0
78-79	32.22659497566043	35.0	33.0	37.0	20.0	39.0
80-81	31.929631858299878	35.0	33.0	36.5	20.0	39.0
82-83	31.682530946208043	35.0	33.0	36.0	20.0	38.5
84-85	31.456114912773895	35.0	33.0	36.0	18.5	37.0
86-87	31.21548107017722	35.0	33.0	35.5	17.5	37.0
88-89	31.06421300313511	35.0	33.0	35.0	16.0	36.5
90-91	30.877380069865154	35.0	33.0	35.0	9.5	36.0
92-93	30.676663379256134	35.0	33.0	35.0	4.5	36.0
94-95	30.51166218762468	35.0	33.0	35.0	2.0	36.0
96-97	30.343341676948054	35.0	33.0	35.0	2.0	36.0
98-99	30.177093551627927	35.0	33.0	35.0	2.0	35.0
100	30.06578806340796	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16367784614067205
1101	2	0.23054570832894683
1101	3	0.16941543277864923
1101	4	0.13646516056497404
1101	5	0.13366558960777297
1101	6	0.13693687580455816
1101	7	0.13354259830601478
1101	8	0.17117196519739508
1101	9	0.24123861815186842
1101	10-11	0.24233146679171824
1101	12-13	0.26346854009881326
1101	14-15	0.32006898447859555
1101	16-17	0.366091605315475
1101	18-19	0.3070036296172347
1101	20-21	0.2390324738387264
1101	22-23	0.26681956546692476
1101	24-25	0.2697024341057883
1101	26-27	0.26229684599343983
1101	28-29	0.3378755913577649
1101	30-31	0.37101647155746065
1101	32-33	0.3177676839304553
1101	34-35	0.38290460318748387
1101	36-37	0.35427461264230686
1101	38-39	0.34276156275421243
1101	40-41	0.39584926791420116
1101	42-43	0.4230358079880858
1101	44-45	0.41378912344161733
1101	46-47	0.3830227948017182
1101	48-49	0.3673587394389273
1101	50-51	0.3654552010770473
1101	52-53	0.3398500227093706
1101	54-55	0.32297380754813787
1101	56-57	0.3218080251633282
1101	58-59	0.3454786230315321
1101	60-61	0.3795040313719156
1101	62-63	0.39582666275141065
1101	64-65	0.42395110692952187
1101	66-67	0.45757811773563617
1101	68-69	0.3005833722748079
1101	70-71	0.2858238577323391
1101	72-73	0.36058905070267144
1101	74-75	0.30829954603246534
1101	76-77	0.3039341163616385
1101	78-79	0.2531378966724631
1101	80-81	0.21271565325295327
1101	82-83	0.21183654663558116
1101	84-85	0.18900560538430256
1101	86-87	0.2198048477803809
1101	88-89	0.19276266965432143
1101	90-91	0.2560420421716678
1101	92-93	0.2962727673493539
1101	94-95	0.2518561362375742
1101	96-97	0.30447443139139807
1101	98-99	0.4183676420829947
1101	100	0.4704824465222188
1102	1	0.10482860936163974
1102	2	0.1790148904667106
1102	3	0.16714484321692424
1102	4	0.13755368436144977
1102	5	0.06431251098213409
1102	6	0.059217070425710006
1102	7	0.13313515925339203
1102	8	0.1278565770403901
1102	9	0.17744370409957355
1102	10-11	0.2132554693987032
1102	12-13	0.2213945495353542
1102	14-15	0.3565909934511069
1102	16-17	0.2801383704889133
1102	18-19	0.2697535168885352
1102	20-21	0.220006019673896
1102	22-23	0.2530788880402426
1102	24-25	0.20275139985405133
1102	26-27	0.25338174716457473
1102	28-29	0.23964263458046986
1102	30-31	0.2504752256790681
1102	32-33	0.32735371607360264
1102	34-35	0.3646245359818323
1102	36-37	0.30245808430082377
1102	38-39	0.3025977771447472
1102	40-41	0.30873278201384835
1102	42-43	0.3796093210268836
1102	44-45	0.46719942622492283
1102	46-47	0.43119398553358224
1102	48-49	0.3240794570645704
1102	50-51	0.3572726527911456
1102	52-53	0.38538802473653533
1102	54-55	0.35230286431272617
1102	56-57	0.3895901833508759
1102	58-59	0.32315293654155397
1102	60-61	0.3078977251408759
1102	62-63	0.33816074959364784
1102	64-65	0.28413759347403555
1102	66-67	0.26159584096238575
1102	68-69	0.32666191648964826
1102	70-71	0.3549877087503148
1102	72-73	0.29392319544172096
1102	74-75	0.24881852892229261
1102	76-77	0.2512563995538528
1102	78-79	0.2666106684832812
1102	80-81	0.25158095940838265
1102	82-83	0.23493971215957998
1102	84-85	0.2548583541682028
1102	86-87	0.22530313132068613
1102	88-89	0.25263009659042623
1102	90-91	0.29210661674386174
1102	92-93	0.20819774201330077
1102	94-95	0.2949748913810524
1102	96-97	0.27463085836598466
1102	98-99	0.2507497882376448
1102	100	0.2557323378682632
1103	1	0.16151887667250975
1103	2	0.15956117198585673
1103	3	0.1806928830725525
1103	4	0.146945637239277
1103	5	0.1285820517845977
1103	6	0.1360731356455247
1103	7	0.16033274151470067
1103	8	0.14862004278509744
1103	9	0.25742271649537685
1103	10-11	0.24840559427286735
1103	12-13	0.2096531509655506
1103	14-15	0.30753595616583596
1103	16-17	0.27788178661710106
1103	18-19	0.29102959653732796
1103	20-21	0.22969076836920266
1103	22-23	0.2576907995466726
1103	24-25	0.253489869945156
1103	26-27	0.24652574440786879
1103	28-29	0.2261913084366327
1103	30-31	0.28545290032330684
1103	32-33	0.23024409227849674
1103	34-35	0.30416493243518516
1103	36-37	0.34436192859278947
1103	38-39	0.258335169747248
1103	40-41	0.29714920689623625
1103	42-43	0.37924724321242564
1103	44-45	0.38803607791085426
1103	46-47	0.4148043948022746
1103	48-49	0.2651573829146159
1103	50-51	0.21744039746693744
1103	52-53	0.26861178736839975
1103	54-55	0.28959490120344356
1103	56-57	0.28773586189281986
1103	58-59	0.3041933532954104
1103	60-61	0.36891710223794405
1103	62-63	0.2850815786456522
1103	64-65	0.2948855683680307
1103	66-67	0.294874779339402
1103	68-69	0.23367111001805085
1103	70-71	0.25006483507258537
1103	72-73	0.23320947115179536
1103	74-75	0.2714179936134613
1103	76-77	0.2047426217538728
1103	78-79	0.2186075099321343
1103	80-81	0.23916105533089294
1103	82-83	0.17170341526410482
1103	84-85	0.18851472587366302
1103	86-87	0.20898797579275197
1103	88-89	0.21500039454986108
1103	90-91	0.2264303547027957
1103	92-93	0.159095232766159
1103	94-95	0.18216589419501616
1103	96-97	0.20491880288209074
1103	98-99	0.21361911437523062
1103	100	0.232846896248887
1104	1	0.12134696993922489
1104	2	0.17992859937967154
1104	3	0.12930319847291827
1104	4	0.1069490913158333
1104	5	0.09192267521220288
1104	6	0.1133479688783936
1104	7	0.15837803309356246
1104	8	0.12207895172866756
1104	9	0.2374847238120381
1104	10-11	0.2741789005714139
1104	12-13	0.26332219397046686
1104	14-15	0.2991301785585563
1104	16-17	0.3050032621720362
1104	18-19	0.31000061384590083
1104	20-21	0.2654274249227271
1104	22-23	0.2453463782954728
1104	24-25	0.3261935317644884
1104	26-27	0.31253738198888925
1104	28-29	0.34312458663759315
1104	30-31	0.26027318040242164
1104	32-33	0.2898524863487779
1104	34-35	0.3222405468207441
1104	36-37	0.2875937652149716
1104	38-39	0.3235529509348183
1104	40-41	0.3077153599350737
1104	42-43	0.26065218885223373
1104	44-45	0.3326873241184245
1104	46-47	0.40591313677113305
1104	48-49	0.3203643202945301
1104	50-51	0.28842308224758284
1104	52-53	0.29425398821298643
1104	54-55	0.29445684608102596
1104	56-57	0.3356957148952944
1104	58-59	0.3464952462289972
1104	60-61	0.31847164260496896
1104	62-63	0.2446174038959512
1104	64-65	0.21155339746430712
1104	66-67	0.20647873292856644
1104	68-69	0.11957615280376643
1104	70-71	0.21507329589953628
1104	72-73	0.19511847948308514
1104	74-75	0.17283150802011704
1104	76-77	0.19396224954390817
1104	78-79	0.20417486691592757
1104	80-81	0.20842666551510192
1104	82-83	0.19423462234761502
1104	84-85	0.2095879141025705
1104	86-87	0.20846421822243144
1104	88-89	0.23345238703724291
1104	90-91	0.26242623305951795
1104	92-93	0.3148892801301848
1104	94-95	0.3008652350974259
1104	96-97	0.3522197243566474
1104	98-99	0.3500240849242431
1104	100	0.42188983473809216
1105	1	0.09909279686498706
1105	2	0.11834020825398284
1105	3	0.13138822626157776
1105	4	0.1093845102110933
1105	5	0.10302587144660436
1105	6	0.12303581034908007
1105	7	0.14175161252291701
1105	8	0.15306383269555823
1105	9	0.17138957922472287
1105	10-11	0.16434642241704012
1105	12-13	0.10229341214444787
1105	14-15	0.2749401140434031
1105	16-17	0.21307379812895988
1105	18-19	0.22493326698541694
1105	20-21	0.16416046195804057
1105	22-23	0.1559240040558194
1105	24-25	0.16175236980878793
1105	26-27	0.15436036747068016
1105	28-29	0.17711682941097706
1105	30-31	0.17210873742764932
1105	32-33	0.18492247312008203
1105	34-35	0.22239181969963795
1105	36-37	0.24025150474236767
1105	38-39	0.22104765775984703
1105	40-41	0.2449832448453222
1105	42-43	0.2792879036570497
1105	44-45	0.3564717714342436
1105	46-47	0.3037150023188957
1105	48-49	0.22361659007561485
1105	50-51	0.15001273586894825
1105	52-53	0.21044523623689315
1105	54-55	0.20882302712387713
1105	56-57	0.26166478148530103
1105	58-59	0.21347281054789136
1105	60-61	0.20957715017760137
1105	62-63	0.21314115313906257
1105	64-65	0.20671493135770191
1105	66-67	0.29374625365738183
1105	68-69	0.22946989436993448
1105	70-71	0.2207172666167594
1105	72-73	0.2583275104059837
1105	74-75	0.2676986538200765
1105	76-77	0.2939529264989389
1105	78-79	0.23779056226212703
1105	80-81	0.2682823137022332
1105	82-83	0.2437885324504805
1105	84-85	0.2068565532730524
1105	86-87	0.23100104465468974
1105	88-89	0.20380592952726317
1105	90-91	0.2344879160083302
1105	92-93	0.2507738744296262
1105	94-95	0.22320448095696577
1105	96-97	0.20238079776283868
1105	98-99	0.21429348272513593
1105	100	0.24639574977310374
1106	1	0.10997591942987128
1106	2	0.1609788043195195
1106	3	0.12514487611066727
1106	4	0.12973974005885225
1106	5	0.08797504177181281
1106	6	0.10756902316525441
1106	7	0.07260392007137284
1106	8	0.0998381635331782
1106	9	0.16909785680741152
1106	10-11	0.14825353252429352
1106	12-13	0.12807299730281585
1106	14-15	0.19437371605678067
1106	16-17	0.19226987860857747
1106	18-19	0.15986436319074926
1106	20-21	0.10913096015287493
1106	22-23	0.1465210660356533
1106	24-25	0.15931946736888136
1106	26-27	0.17660599358233497
1106	28-29	0.12143337508481267
1106	30-31	0.14944833122291357
1106	32-33	0.13205591582436682
1106	34-35	0.09677097219060471
1106	36-37	0.18846349186037514
1106	38-39	0.16456153542797125
1106	40-41	0.19162659993196485
1106	42-43	0.18520894639420504
1106	44-45	0.08106626702407027
1106	46-47	0.09372358866400532
1106	48-49	0.2051086316699866
1106	50-51	0.2261496008347521
1106	52-53	0.2024861712533692
1106	54-55	0.18968596752336708
1106	56-57	0.24372757153975755
1106	58-59	0.23578162464919927
1106	60-61	0.18054959245886693
1106	62-63	0.19011113251732326
1106	64-65	0.17986466692420322
1106	66-67	0.19352671110827657
1106	68-69	0.23133798252617055
1106	70-71	0.1704904758950363
1106	72-73	0.17000883307550652
1106	74-75	0.1771696998587018
1106	76-77	0.11171945508768744
1106	78-79	0.13673069009558958
1106	80-81	0.16316624356629106
1106	82-83	0.07581016161299203
1106	84-85	0.13445960095303278
1106	86-87	0.16481610752226317
1106	88-89	0.14454352316597863
1106	90-91	0.1199171800445491
1106	92-93	0.15719393744681298
1106	94-95	0.18611660048245682
1106	96-97	0.1715543839460949
1106	98-99	0.19278257986908187
1106	100	0.1870669842485242
1107	1	0.11055922891803505
1107	2	0.19136681401560907
1107	3	0.12207543203027171
1107	4	0.13781376997538786
1107	5	0.11893834253873337
1107	6	0.12429929906593173
1107	7	0.06276902748490443
1107	8	0.06991971721573975
1107	9	0.10970990442478268
1107	10-11	0.09238571090990888
1107	12-13	0.08178328149708847
1107	14-15	0.18175369910450456
1107	16-17	0.2216036638485761
1107	18-19	0.14363371973371386
1107	20-21	0.16188470486766704
1107	22-23	0.1491303015576193
1107	24-25	0.1854521728439451
1107	26-27	0.21326341851681008
1107	28-29	0.20704692812966385
1107	30-31	0.25335344321605646
1107	32-33	0.26651154714215863
1107	34-35	0.31838001159545115
1107	36-37	0.33651946758132567
1107	38-39	0.20884155945770289
1107	40-41	0.2837605386031612
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2216	76-77	-0.8859533994189448
2216	78-79	-0.846954710211314
2216	80-81	-0.8511164014873813
2216	82-83	-0.823956965902191
2216	84-85	-0.8391679957795475
2216	86-87	-0.9278508322332897
2216	88-89	-0.9158929975311452
2216	90-91	-0.9455286759890846
2216	92-93	-0.8134756628060806
2216	94-95	-0.856277303931698
2216	96-97	-1.0822014499136685
2216	98-99	-1.1808385995536383
2216	100	-1.169766221013088
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	229.0
3	744.0
4	555.0
5	478.0
6	1015.0
7	2083.0
8	3734.0
9	5328.0
10	7228.0
11	9716.0
12	13461.0
13	19216.0
14	18706.0
15	22630.0
16	28342.0
17	40225.0
18	45133.0
19	25638.0
20	16872.0
21	15464.0
22	15280.0
23	15679.0
24	17122.0
25	20324.0
26	24755.0
27	30702.0
28	38120.0
29	47449.0
30	59504.0
31	73948.0
32	93105.0
33	118812.0
34	160332.0
35	251733.0
36	363505.0
37	685329.0
38	1011621.0
39	490137.0
40	3061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14238232708209	20.535577138668675	14.107365726973061	23.21467480727617
2	14.7423376780699	22.06911989129161	39.39473022385554	23.79381220678295
3	16.24846667611196	28.154302023592514	31.63358444118014	23.963646859115386
4	11.078902590100922	18.77665712620994	38.22045227615129	31.923988007537847
5	10.86547516965454	37.53183985369996	35.02033642268578	16.58234855395973
6	26.60896623244746	39.10364009929124	19.06052541348964	15.226868254771661
7	23.71975988296994	34.24008806222291	23.84339987596499	18.196752178842154
8	24.790411119435706	33.129540214599004	22.31384544079172	19.766203225173577
9	24.901015586012747	15.592254000524056	23.815353743368668	35.69137667009453
10-11	22.391188921915813	26.814900674286562	29.755350546313807	21.03855985748381
12-13	24.5294504143059	25.228141463112753	30.02381946190927	20.21858866067208
14-15	21.88323855145017	26.400403969646973	29.66449978471631	22.05185769418655
16-17	18.880551126256314	29.368711839813134	30.123205475447783	21.627531558482772
18-19	18.54062673230954	26.20296182960855	35.19307721376815	20.06333422431376
20-21	20.38856402484387	25.61286066602323	34.938831779823374	19.059743529309525
22-23	19.831486194850836	25.979264295956483	35.038770815694775	19.150478693497906
24-25	19.436982245482103	25.770451377522953	35.281254748455936	19.51131162853901
26-27	19.231549134059197	25.54888124898777	35.40971712907673	19.809852487876302
28-29	19.37188306699295	25.61980511165492	35.343549796204016	19.664762025148114
30-31	19.900057279424836	26.24451072178659	34.72000895402503	19.13542304476354
32-33	19.063848568711613	25.85817919144405	35.38328286343528	19.694689376409055
34-35	19.29050685681149	25.354296245226145	35.11317149323484	20.24202540472753
36-37	20.11969760494181	26.050471511280033	34.144840659525876	19.68499022425228
38-39	19.9340945492774	25.965960174420594	34.41805903101659	19.681886245285412
40-41	19.553091677921472	25.8112513303978	34.47910490348781	20.156552088192914
42-43	19.49134007464647	26.003912059638175	34.25004921347323	20.254698652242126
44-45	20.087577681543102	26.181924093988002	33.665146776754995	20.0653514477139
46-47	20.044792405542314	26.872493857799583	32.8161977386274	20.266515998030705
48-49	20.01390199946251	26.46995736834281	32.765872447904044	20.750268184290636
50-51	20.063690703092956	26.358562725862022	32.462586485114585	21.115160085930437
52-53	20.114971663295616	26.702391327905854	32.10173799243591	21.08089901636262
54-55	20.394625092994318	27.400451639662656	31.708198642447545	20.496724624895485
56-57	19.93195526750821	26.884292878097032	31.818543341810944	21.365208512583813
58-59	19.799589407828115	26.20110061878532	32.5014913266025	21.49781864678407
60-61	20.376607801507713	26.84115360893864	31.75749341763258	21.02474517192106
62-63	20.09747165449127	26.630010772392808	32.0672183617088	21.205299211407123
64-65	19.99041152744552	26.398518619175682	32.4282747491091	21.1827951042697
66-67	19.96840661167428	26.52768858826115	31.905069202213614	21.598835597850954
68-69	20.625708731359673	26.728540812655805	31.650342692844717	20.995407763139806
70-71	20.728446335963362	26.410574247340413	31.61931329647031	21.24166612022592
72-73	20.304280787872482	26.523227596341098	32.02005891004565	21.152432705740768
74-75	20.28430689660167	26.560257867092584	31.675543576299507	21.479891660006242
76-77	20.406761093035474	26.68041761086452	31.217834180203646	21.694987115896364
78-79	20.584471391407753	26.835448238212763	31.352374697300935	21.227705673078557
80-81	20.165406346326286	26.625339219948835	31.5076442170323	21.701610216692586
82-83	19.963934639929054	26.668931450139542	31.650335866072293	21.71679804385911
84-85	20.159629651623874	26.57984069953055	31.3863875798777	21.874142068967878
86-87	20.334486341006738	26.766662760397807	31.09443119677983	21.804419701815625
88-89	19.90939650779564	26.694150998797834	31.244076406618888	22.152376086787637
90-91	19.76317476954111	26.534116869419577	31.094655039152663	22.608053321886647
92-93	19.957827980347638	26.85007220253209	30.863787721000513	22.32831209611976
94-95	19.870500603716046	26.54187234927837	31.1279680511098	22.459658995895783
96-97	19.755723610768396	26.682585007177472	31.029993176725444	22.531698205328684
98-99	19.643990939856863	26.619582223221766	31.01129199737392	22.725134839547458
100	19.868385397094094	26.81282142912633	30.627480216859404	22.691312956920168
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4041.0
1	2649.0
2	10648.0
3	18426.0
4	10701.5
5	4566.5
6	7467.0
7	16135.0
8	24653.0
9	25832.5
10	17214.5
11	8284.0
12	6332.5
13	6429.5
14	6824.5
15	7186.5
16	7685.5
17	8447.0
18	9047.0
19	9698.5
20	10647.0
21	12071.0
22	14274.5
23	17530.0
24	21799.5
25	26777.5
26	34001.5
27	43571.5
28	55146.5
29	66091.5
30	73560.0
31	80860.0
32	88877.5
33	95755.0
34	101774.0
35	107336.0
36	111689.0
37	115132.5
38	117912.0
39	118864.5
40	119102.0
41	118578.0
42	115907.0
43	114745.0
44	114564.5
45	112882.0
46	112043.5
47	112023.0
48	112155.0
49	113396.5
50	115476.0
51	111749.0
52	102521.5
53	95506.5
54	90983.5
55	87857.5
56	81300.5
57	72356.0
58	66896.5
59	61946.5
60	53350.5
61	43817.5
62	35893.5
63	28064.5
64	21281.5
65	16048.5
66	12023.0
67	8751.5
68	6183.5
69	4437.0
70	3212.5
71	2300.0
72	1691.0
73	1310.0
74	994.0
75	715.0
76	426.5
77	301.0
78	252.5
79	173.5
80	92.0
81	32.0
82	13.5
83	8.0
84	8.5
85	8.5
86	3.0
87	2.0
88	3.0
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	1.0
95	2.5
96	1.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04892930926193902
2	0.0
3	2.3700957123651845E-4
4	0.06841676289694165
5	0.004924532202358772
6	2.63343968040576E-5
7	0.0
8	0.0
9	0.0
10-11	0.01087610588007579
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.373631105136129E-4
26-27	0.0
28-29	3.2917996005072005E-4
30-31	0.0036868155525680646
32-33	5.26687936081152E-5
34-35	0.0018170733794799748
36-37	0.004292506679061389
38-39	0.0015010606178312833
40-41	0.0012772182449967938
42-43	0.0021725877363347524
44-45	1.31671984020288E-5
46-47	5.135207376791232E-4
48-49	5.135207376791232E-4
50-51	0.0015142278162333122
52-53	8.295334993278146E-4
54-55	0.0010533758721623042
56-57	0.006688936788230631
58-59	0.009480382849460737
60-61	0.0012903854433988227
62-63	0.00285728205324025
64-65	0.0012377166497907074
66-67	3.9501595206086404E-5
68-69	9.743726817501313E-4
70-71	3.6868155525680645E-4
72-73	0.0
74-75	2.6334396804057606E-4
76-77	0.0
78-79	5.398551344831809E-4
80-81	0.0
82-83	0.0011192118641724482
84-85	6.71527118503469E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.765111664426048E-4
94-95	0.0
96-97	4.740191424730369E-4
98-99	6.320255232973825E-4
100	4.213503488649216E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3797315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.872971439293934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70477107894985	44.569648255492986
2	15.740233866942537	18.533486777926303
3	4.456144629598358	7.870394265231216
4	1.5944211202233574	3.7547323629246705
5	0.7737462911825495	2.277637165102492
6	0.43502543938022015	1.5366744160798798
7	0.25316394143219384	1.0433159445377642
8	0.17491128543454051	0.8238037689438313
9	0.11829391597552652	0.6267882903002467
>10	0.659246361741573	7.095053453478719
>50	0.05291918763227229	2.1449456491490215
>100	0.0338126704298394	3.7020094367000276
>500	0.002236629118508583	0.9167879566186673
>1k	7.15721305679043E-4	0.65340760920983
>5k	1.3419774481482016E-4	0.5941529592270746
>10k+	2.2366290802470023E-4	3.8571616890773908
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40718	1.0722839690676176	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35037	0.9226782608237662	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29287	0.771255479200435	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25938	0.683061584303646	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15483	0.40773546571722386	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9044	0.23816828469589696	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7847	0.20664601172144	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5670	0.1493160298790066	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.266879360811521E-5	0.0
6	0.0	0.0	0.0	5.266879360811521E-5	0.0
7	0.0	0.0	0.0	5.266879360811521E-5	0.0
8	0.0	0.0	0.0	5.266879360811521E-5	0.0
9	0.0	0.0	0.0	5.266879360811521E-5	0.0
10-11	0.0	0.0	0.0	5.266879360811521E-5	0.0
12-13	0.0	0.0	0.0	5.266879360811521E-5	0.0
14-15	0.0	0.0	0.0	6.583599201014402E-5	0.0
16-17	0.0	0.0	0.0	7.900319041217281E-5	0.0
18-19	0.0	0.0	0.0	7.900319041217281E-5	0.0
20-21	0.0	0.0	0.0	3.2917996005072005E-4	0.0
22-23	0.0	0.0	0.0	0.0012245494513886787	0.0
24-25	0.0	0.0	0.0	0.002804613259632135	0.0
26-27	0.0	0.0	0.0	0.004779693019936455	0.0
28-29	0.0	0.0	0.0	0.01009924117435609	0.0
30-31	0.0	0.0	0.0	0.01996147277747566	0.0
32-33	0.0	0.0	0.0	0.039067077658819456	0.0
34-35	0.0	0.0	0.0	0.06978615153075265	0.0
36-37	0.0	0.0	0.0	0.11546316278739056	0.0
38-39	0.0	0.0	0.0	0.17808635838743955	0.0
40-41	0.0	0.0	0.0	0.2511248079234933	0.0
42-43	0.0	0.0	0.0	0.32642801558469603	0.0
44-45	0.0	0.0	0.0	0.4221535479674454	0.0
46-47	0.0	0.0	0.0	0.5350227726696363	0.0
48-49	0.0	0.0	0.0	0.6576225569909264	0.0
50-51	0.0	0.0	0.0	0.7878329819885893	0.0
52-53	0.0	0.0	0.0	0.9163711727891946	0.0
54-55	0.0	0.0	0.0	1.0639754668759371	0.0
56-57	0.0	0.0	0.0	1.2385856848852412	0.0
58-59	0.0	0.0	0.0	1.4366861848437646	0.0
60-61	0.0	0.0	0.0	1.6419364735345896	0.0
62-63	0.0	0.0	0.0	1.852374638395814	0.0
64-65	0.0	0.0	0.0	2.0839856582875003	0.0
66-67	0.0	0.0	0.0	2.357626375478463	0.0
68-69	0.0	0.0	0.0	2.6587338685360575	0.0
70-71	0.0	0.0	0.0	2.987610983023531	0.0
72-73	0.0	0.0	0.0	3.3484580552311307	0.0
74-75	0.0	0.0	0.0	3.7151513635292304	0.0
76-77	0.0	0.0	0.0	4.099291209710019	0.0
78-79	0.0	0.0	0.0	4.5347699624603175	0.0
80-81	0.0	0.0	0.0	5.005892321284908	0.0
82-83	0.0	0.0	0.0	5.502638048199847	0.0
84-85	0.0	0.0	0.0	5.9972901905688625	0.0
86-87	0.0	0.0	0.0	6.524715489760528	0.0
88	0.0	0.0	0.0	6.947119214497612	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13260	0.0	61.386723	1
TACCTGG	3530	0.0	42.497063	2
GTATCAA	24290	0.0	41.29824	1
TATAACG	480	0.0	38.209057	2
ATAACGC	535	0.0	36.03902	3
GTGGTAT	4585	0.0	35.814426	1
GTACCTG	4285	0.0	35.357124	1
TGGTATC	4610	0.0	35.2954	2
ACCTGGG	4695	0.0	31.351019	3
ATCAACG	32165	0.0	31.141462	3
TATCAAC	32290	0.0	31.020908	2
TCAACGC	32420	0.0	30.910614	4
CAACGCA	33725	0.0	29.758532	5
AACGCAG	35065	0.0	28.807858	6
ACGCAGA	39040	0.0	25.778362	7
CGCAGAG	38955	0.0	25.774275	8
TAACGCA	830	0.0	24.9294	4
GCAGAGT	40835	0.0	24.31139	9
CATGGGT	6780	0.0	23.374235	4
TTTCGCG	535	0.0	22.84378	9
>>END_MODULE