FastQCFastQC Report
Wed 25 May 2016
SRR2431462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035806
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA444052.1811999768150794No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55490.272570176136626No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33680.16543816061058864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22150.043.2925642
GGTATCA39950.042.8325081
GTACCTG24350.039.96321
GTCTCGC5800.038.094191
TCTGTCG6850.034.99238
GTATCAA62250.032.095081
TATAACG1452.6588168E-829.176382
CGCTCTG6350.028.8693565
TCTCGCT7350.028.7794192
ACCTGGG32200.028.7585663
TCGCTCT7400.028.584264
CTGTCGC9250.026.4213189
ATCAACG73950.026.3795133
TCAACGC73500.026.1566544
CTCGCTC7850.025.748723
GTACATG115200.025.62691
TATCAAC79100.025.3751282
CATGGGT46150.025.3614354
TACATGG114500.025.3300782
CAACGCA79150.024.4082785
Produced by FastQC (version 0.11.5)