##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431462_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2035806
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.9793560879573	31.0	30.0	34.0	18.0	34.0
2	29.069722753543314	31.0	30.0	34.0	16.0	34.0
3	29.1119644995643	31.0	30.0	34.0	16.0	34.0
4	32.18898952061247	37.0	33.0	37.0	17.0	37.0
5	32.507279672031615	37.0	33.0	37.0	20.0	37.0
6	32.34149815846893	37.0	33.0	37.0	17.0	37.0
7	32.28689128531894	37.0	33.0	37.0	17.0	37.0
8	32.13906826092467	37.0	33.0	37.0	16.0	37.0
9	33.26956841663695	39.0	34.0	39.0	13.0	39.0
10-11	33.280797138823644	39.0	34.0	39.0	12.5	39.0
12-13	33.24119488792154	39.0	34.0	39.0	11.0	39.0
14-15	34.32764467734155	40.0	34.0	41.0	11.5	41.0
16-17	34.33337385782339	40.0	34.0	41.0	12.0	41.0
18-19	34.27570824528466	40.0	34.0	41.0	13.0	41.0
20-21	34.35941563194136	40.0	34.0	41.0	13.0	41.0
22-23	34.32920794024578	40.0	34.0	41.0	16.0	41.0
24-25	34.386667737495614	40.0	34.0	41.0	17.5	41.0
26-27	34.412930063080665	40.0	34.0	41.0	18.0	41.0
28-29	34.3269731988215	40.0	33.0	41.0	17.5	41.0
30-31	34.30563079193204	39.0	33.0	41.0	18.0	41.0
32-33	34.24271246867334	39.0	33.0	41.0	17.0	41.0
34-35	34.13708108729417	39.0	33.0	41.0	16.0	41.0
36-37	33.999999263191086	39.0	33.0	41.0	15.5	41.0
38-39	33.8802066601631	39.0	33.0	41.0	14.5	41.0
40-41	33.79274670572737	39.0	33.0	41.0	10.5	41.0
42-43	33.55898032523727	39.0	33.0	41.0	8.5	41.0
44-45	33.40989858562162	38.5	32.0	41.0	8.0	41.0
46-47	33.26459299167013	38.0	32.0	40.5	6.5	41.0
48-49	33.11817064101393	38.0	32.0	40.0	2.0	41.0
50-51	32.95053973708693	38.0	32.0	40.0	2.0	41.0
52-53	32.85281849056344	38.0	31.5	40.0	2.0	41.0
54-55	32.780996814038275	38.0	31.0	40.0	2.0	41.0
56-57	32.62888335136059	38.0	31.0	40.0	2.0	41.0
58-59	32.41705594737416	37.0	31.0	40.0	2.0	41.0
60-61	32.192422804530494	37.0	31.0	40.0	2.0	41.0
62-63	31.903132715003295	36.5	31.0	40.0	2.0	41.0
64-65	31.746027617562774	36.0	31.0	40.0	2.0	41.0
66-67	31.4844152635369	36.0	30.5	39.5	2.0	41.0
68-69	31.21163362324308	35.0	30.0	39.0	2.0	41.0
70-71	30.918171721666994	35.0	30.0	39.0	2.0	41.0
72-73	30.607897314380644	35.0	30.0	38.0	2.0	40.0
74-75	30.290173769013354	35.0	30.0	37.0	2.0	40.0
76-77	29.988417118330528	35.0	30.0	37.0	2.0	39.0
78-79	29.667626974279475	35.0	29.0	36.0	2.0	39.0
80-81	29.39009267091265	35.0	29.0	36.0	2.0	39.0
82-83	29.10751491055631	35.0	29.0	36.0	2.0	37.0
84-85	28.83092593302112	34.0	29.0	35.0	2.0	37.0
86-87	28.584706253935785	34.0	29.0	35.0	2.0	37.0
88-89	28.33662269391091	34.0	28.0	35.0	2.0	36.0
90-91	28.126016182288488	34.0	28.0	35.0	2.0	36.0
92-93	27.939459850300075	34.0	28.0	35.0	2.0	36.0
94-95	27.71334351112041	34.0	27.5	35.0	2.0	35.0
96-97	27.623859542608677	34.0	27.5	35.0	2.0	35.0
98-99	27.469736556430227	34.0	27.0	35.0	2.0	35.0
100	27.33348560717475	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5550439031313879
1101	2	0.5264164792951931
1101	3	0.4671388656144231
1101	4	0.5097080716721223
1101	5	0.2980888389901608
1101	6	0.3286056427061652
1101	7	0.4433593581272248
1101	8	0.5333803262167933
1101	9	0.6189177423279375
1101	10-11	0.5973576154195044
1101	12-13	0.5731192233928653
1101	14-15	0.6626299108445508
1101	16-17	0.5411157237321831
1101	18-19	0.5205917281586352
1101	20-21	0.43348920436620375
1101	22-23	0.3199886846713724
1101	24-25	0.3708503585448568
1101	26-27	0.38926165737446183
1101	28-29	0.5626861621261341
1101	30-31	0.5940363433006723
1101	32-33	0.5802001072656893
1101	34-35	0.5891322932792846
1101	36-37	0.6313127468109414
1101	38-39	0.594046398168004
1101	40-41	0.5448024063270651
1101	42-43	0.658633268537514
1101	44-45	0.5155837066356028
1101	46-47	0.5131142249182474
1101	48-49	0.4322807201255685
1101	50-51	0.42434466321294195
1101	52-53	0.3905201952899091
1101	54-55	0.4473863734596648
1101	56-57	0.5126819056391483
1101	58-59	0.4823259020241224
1101	60-61	0.5261567899453894
1101	62-63	0.5145682379100727
1101	64-65	0.4873035168856923
1101	66-67	0.4421486008605555
1101	68-69	0.41886624031011266
1101	70-71	0.4360093617618652
1101	72-73	0.5035031357427755
1101	74-75	0.5258379352626896
1101	76-77	0.45191920928901297
1101	78-79	0.346319402750062
1101	80-81	0.39030221500331663
1101	82-83	0.31943533143295966
1101	84-85	0.3908790633004422
1101	86-87	0.475729666066119
1101	88-89	0.41636139223430746
1101	90-91	0.3831209901568222
1101	92-93	0.28210599093984
1101	94-95	0.2148779764933586
1101	96-97	0.33517712750177253
1101	98-99	0.32866922071110594
1101	100	0.3872558926842906
1102	1	0.17889803926061632
1102	2	0.31250731108426777
1102	3	0.21378548291578525
1102	4	0.17205307715564544
1102	5	0.2046158210143716
1102	6	0.20918127509764872
1102	7	0.18263264598460438
1102	8	0.30295339355464534
1102	9	0.4452878008516947
1102	10-11	0.36587540611292724
1102	12-13	0.3180426635947242
1102	14-15	0.4384911440311683
1102	16-17	0.3572039422354365
1102	18-19	0.3362917807766763
1102	20-21	0.34332677364835007
1102	22-23	0.3076815785142557
1102	24-25	0.3475223785394732
1102	26-27	0.3314365429323445
1102	28-29	0.516188809490373
1102	30-31	0.46936821298336895
1102	32-33	0.3822480801954029
1102	34-35	0.41494330788416534
1102	36-37	0.31510054605858784
1102	38-39	0.3838908365891669
1102	40-41	0.43505946690657993
1102	42-43	0.3969804861413593
1102	44-45	0.3323983845788021
1102	46-47	0.300452593919303
1102	48-49	0.24435011568142073
1102	50-51	0.2859542917679576
1102	52-53	0.2750352558225231
1102	54-55	0.19720404483611986
1102	56-57	0.2652732318385702
1102	58-59	0.28363471963439224
1102	60-61	0.29304805016268176
1102	62-63	0.28085920778657325
1102	64-65	0.30734513507212924
1102	66-67	0.26799527327053596
1102	68-69	0.32516378531579093
1102	70-71	0.33128779454848
1102	72-73	0.33959422873893885
1102	74-75	0.3650375141239621
1102	76-77	0.28314310300713075
1102	78-79	0.19205468673744974
1102	80-81	0.2623013251983295
1102	82-83	0.3109034574251659
1102	84-85	0.24108639490964734
1102	86-87	0.24027388945916783
1102	88-89	0.309297344134297
1102	90-91	0.24594152618535858
1102	92-93	0.21347534663428291
1102	94-95	0.18705571589056902
1102	96-97	0.16808252667662416
1102	98-99	0.23454573633487286
1102	100	0.23864594569960929
1103	1	-0.10685145771355309
1103	2	0.031770770487128175
1103	3	-0.10772659082614311
1103	4	-0.150497939649199
1103	5	-0.22315212608633317
1103	6	-0.145323293618155
1103	7	-0.0457894443282143
1103	8	0.011710265170357559
1103	9	0.003562680597312351
1103	10-11	0.09177436585893162
1103	12-13	-0.12684670379087493
1103	14-15	0.10865760522008117
1103	16-17	0.0064995407284058615
1103	18-19	-0.03361304910983165
1103	20-21	-0.05062037028572064
1103	22-23	-0.17229122023915266
1103	24-25	-0.03650260522445592
1103	26-27	0.020517743826275137
1103	28-29	0.16482368649129597
1103	30-31	0.0637419058159594
1103	32-33	0.1670836450458495
1103	34-35	0.09704663081310372
1103	36-37	0.11148453525745339
1103	38-39	0.10760585463691541
1103	40-41	0.09484931153043874
1103	42-43	0.08235482038577402
1103	44-45	0.08395919073814895
1103	46-47	-0.00901077551346674
1103	48-49	-0.03994767183163361
1103	50-51	-0.12242036643016974
1103	52-53	-0.08301662377629526
1103	54-55	-0.02019859986862116
1103	56-57	0.09759854514243926
1103	58-59	0.11896248036545387
1103	60-61	0.061853811271440406
1103	62-63	0.003426569904586785
1103	64-65	0.003511863880113708
1103	66-67	-0.032393908163502516
1103	68-69	-0.0494259136647166
1103	70-71	0.05654876743238546
1103	72-73	0.005031886899626414
1103	74-75	-0.029365267224754632
1103	76-77	-0.019811607602289172
1103	78-79	-0.0011457928982849808
1103	80-81	-0.020838000054389738
1103	82-83	0.02676794202401922
1103	84-85	-0.009077264900451354
1103	86-87	0.06936208671402966
1103	88-89	0.03696612100766217
1103	90-91	0.0498649091833272
1103	92-93	0.028257733709011745
1103	94-95	0.0012312359732753464
1103	96-97	-0.1227133495331394
1103	98-99	-0.1580598282285841
1103	100	-0.046422102886122474
1104	1	0.15405457371875286
1104	2	0.15717416119005634
1104	3	0.22364716545741103
1104	4	0.1976543969830047
1104	5	0.17512151969553003
1104	6	0.28857810297117226
1104	7	0.3416788030362099
1104	8	0.3284692226535384
1104	9	0.5833468016005554
1104	10-11	0.43092187943350524
1104	12-13	0.305339523842612
1104	14-15	0.3272421875552922
1104	16-17	0.3276549245145617
1104	18-19	0.27281614119624464
1104	20-21	0.23605389518485964
1104	22-23	0.2943952448378724
1104	24-25	0.2259155546041285
1104	26-27	0.2533286746553358
1104	28-29	0.36069297027273706
1104	30-31	0.3794232657423535
1104	32-33	0.3643945912160049
1104	34-35	0.33388998035610484
1104	36-37	0.3298624707305109
1104	38-39	0.3460976279165706
1104	40-41	0.32957862360360934
1104	42-43	0.31290660299831075
1104	44-45	0.2936295655234318
1104	46-47	0.21234190164528854
1104	48-49	0.14162027525944865
1104	50-51	0.08514678979302204
1104	52-53	0.04907794652874031
1104	54-55	0.07148689207913606
1104	56-57	0.15833011166990474
1104	58-59	0.1920910840921053
1104	60-61	0.17781641700479867
1104	62-63	0.23043988277091287
1104	64-65	0.21103877834594087
1104	66-67	0.2687158713505937
1104	68-69	0.28677885700420447
1104	70-71	0.168781540411171
1104	72-73	0.2081004954896315
1104	74-75	0.23189177765870994
1104	76-77	0.24065497010128212
1104	78-79	0.19894790227732528
1104	80-81	0.18830419451010272
1104	82-83	0.1094092850431565
1104	84-85	0.18290242119781297
1104	86-87	0.23906901910375566
1104	88-89	0.18943893406563816
1104	90-91	0.14189574753533662
1104	92-93	0.031960719544372296
1104	94-95	0.056042284035786594
1104	96-97	0.14970371352151446
1104	98-99	0.09907464037643621
1104	100	0.10153773732833926
1105	1	0.2590335124691805
1105	2	0.2683126207948767
1105	3	0.24516228364654324
1105	4	0.019841328010684833
1105	5	0.06316478734010644
1105	6	0.21611666871142177
1105	7	0.22311129968019827
1105	8	0.15886375667884778
1105	9	0.015227668070657785
1105	10-11	0.15388112901411688
1105	12-13	0.2028985398554468
1105	14-15	0.2426958466964706
1105	16-17	0.19896113221532374
1105	18-19	0.13443509056021696
1105	20-21	0.17744243639874924
1105	22-23	0.18454921976687189
1105	24-25	0.24756356028795778
1105	26-27	0.2799559245780898
1105	28-29	0.3245327337024406
1105	30-31	0.2785527108181469
1105	32-33	0.25180418844251307
1105	34-35	0.14555479663532367
1105	36-37	0.16505317410467768
1105	38-39	0.16558491348546767
1105	40-41	0.08394994574035053
1105	42-43	0.13744841389919316
1105	44-45	0.009256436640455945
1105	46-47	0.07718899156189707
1105	48-49	-0.011949881482337332
1105	50-51	-0.09496202520838182
1105	52-53	-0.09870036658125514
1105	54-55	-0.12561122907933964
1105	56-57	-0.12684274244799099
1105	58-59	-0.1601561329077157
1105	60-61	-0.15829097005610748
1105	62-63	-0.14968905082985273
1105	64-65	-0.11884842657399375
1105	66-67	-0.18935015955087664
1105	68-69	-0.17247465131293538
1105	70-71	-0.08225079249098499
1105	72-73	-0.23286865366381448
1105	74-75	-0.11510976549459073
1105	76-77	-0.2420980223767195
1105	78-79	-0.2011381581954268
1105	80-81	-0.19895440411816878
1105	82-83	-0.29407708056896453
1105	84-85	-0.2594189509517655
1105	86-87	-0.21573592212898518
1105	88-89	-0.2010253298903848
1105	90-91	-0.2336927542268974
1105	92-93	-0.23075000205436424
1105	94-95	-0.21858096136923066
1105	96-97	-0.06482012592769593
1105	98-99	-0.020816500980949115
1105	100	-0.04827120143954744
1106	1	0.09063466239465967
1106	2	0.19661575119469532
1106	3	0.11870674485861343
1106	4	0.2089996582265954
1106	5	0.2017506996502334
1106	6	0.2457031162848864
1106	7	0.2498793440349516
1106	8	0.34507835062095893
1106	9	0.42736007745973836
1106	10-11	0.2546370294229021
1106	12-13	0.24536566272595195
1106	14-15	0.21058471211633645
1106	16-17	0.14228726366954447
1106	18-19	0.06592912419782948
1106	20-21	0.26563915882272227
1106	22-23	0.2009728336172074
1106	24-25	0.23203700028375351
1106	26-27	0.29739980463499194
1106	28-29	0.37654552742925773
1106	30-31	0.38066914289356646
1106	32-33	0.24000284069864364
1106	34-35	0.20891330685579135
1106	36-37	0.2548619425833465
1106	38-39	0.15684489453494166
1106	40-41	0.17007421929638866
1106	42-43	0.1360469528253887
1106	44-45	0.20675386847920407
1106	46-47	0.12752982960953574
1106	48-49	0.11656453372762599
1106	50-51	0.21634195601414064
1106	52-53	0.18007989891275855
1106	54-55	0.17911690210513598
1106	56-57	0.2546066081528906
1106	58-59	0.3200767660225239
1106	60-61	0.366482136820963
1106	62-63	0.34929874160333796
1106	64-65	0.33445334658123116
1106	66-67	0.3275600476918896
1106	68-69	0.3642715795764211
1106	70-71	0.37702105426931354
1106	72-73	0.38186135221475226
1106	74-75	0.31794785632447287
1106	76-77	0.4049381159570409
1106	78-79	0.30320481242277
1106	80-81	0.2785000666879256
1106	82-83	0.2723096756079819
1106	84-85	0.3145207653332207
1106	86-87	0.31206786950343
1106	88-89	0.35569052840988746
1106	90-91	0.2869862455517058
1106	92-93	0.31580582014320413
1106	94-95	0.3357924836314048
1106	96-97	0.3396625136415814
1106	98-99	0.3551418409412008
1106	100	0.33604872146895914
1107	1	-0.06164773411544999
1107	2	-0.12283178261028738
1107	3	-0.09630991278896417
1107	4	-0.04781217426403117
1107	5	-0.18769070062133864
1107	6	-0.12108467175879412
1107	7	0.05793834215256766
1107	8	-0.049724289039353664
1107	9	-0.003837903703093559
1107	10-11	0.11230087527284383
1107	12-13	0.013900304325083823
1107	14-15	0.029587170509564942
1107	16-17	-0.04019116521632071
1107	18-19	-0.007533321812132954
1107	20-21	-0.06372287070455229
1107	22-23	-0.009957222522466225
1107	24-25	0.05587397362155855
1107	26-27	0.04925563423066137
1107	28-29	0.09987990791748302
1107	30-31	0.0325367581691296
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2216	48-49	-0.4812614227098493
2216	50-51	-0.4306417366578543
2216	52-53	-0.3952455311849121
2216	54-55	-0.6742176106563065
2216	56-57	-0.5733975513324765
2216	58-59	-0.5897485171803147
2216	60-61	-0.5086244319798503
2216	62-63	-0.6309815273382888
2216	64-65	-0.6906544630206426
2216	66-67	-0.6690354543642378
2216	68-69	-0.7139970506512547
2216	70-71	-0.6870126859133485
2216	72-73	-0.7170198855123253
2216	74-75	-0.5678142917183067
2216	76-77	-0.5265241360728687
2216	78-79	-0.5095680137137819
2216	80-81	-0.5486538438767354
2216	82-83	-0.5926479410293162
2216	84-85	-0.6302621865405094
2216	86-87	-0.546653621090897
2216	88-89	-0.5369685723055362
2216	90-91	-0.7191519172461973
2216	92-93	-0.6296255503495871
2216	94-95	-0.39695888064937535
2216	96-97	-0.48387266066695034
2216	98-99	-0.7199177175097375
2216	100	-0.7487944532846598
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	98931.0
3	20500.0
4	14855.0
5	13316.0
6	8603.0
7	6727.0
8	7106.0
9	7120.0
10	7258.0
11	7717.0
12	8284.0
13	8939.0
14	9273.0
15	9253.0
16	8936.0
17	8796.0
18	8432.0
19	7900.0
20	7750.0
21	8368.0
22	10587.0
23	18378.0
24	29376.0
25	33970.0
26	37071.0
27	27542.0
28	26676.0
29	30625.0
30	34849.0
31	39288.0
32	49818.0
33	64826.0
34	88405.0
35	144130.0
36	208924.0
37	350576.0
38	412751.0
39	149619.0
40	331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56562839438789	22.548634777495185	12.673462279338688	21.21227454877823
2	16.241241745269694	24.71401040748144	37.12394708130225	21.92080076594662
3	17.299751966606618	31.332772977967814	28.51946809142995	22.848006963995612
4	12.23910423844007	22.067555465602556	36.11012997935284	29.58321031660453
5	11.559738670489308	41.323478046424846	31.79243767534834	15.324345607737508
6	25.978374607603765	41.94653094710573	18.149609200371394	13.925485244919114
7	24.24680535823415	37.655310778759635	20.850158872389216	17.247724990617
8	23.83029164539999	38.0088590563145	20.027146027056624	18.133703271228896
9	24.205005320105283	22.44578454113969	20.32432501471257	33.024885124042456
10-11	21.635838864424066	32.178483015214255	26.477932906762057	19.70774521359962
12-13	22.61280773561215	30.812270714986077	25.948480995993467	20.626440553408305
14-15	20.991155181010534	32.27774352726764	24.323399593742554	22.407701697979274
16-17	19.707306338028168	34.90253041687626	24.620771504024145	20.769391741071427
18-19	19.553400023186217	33.26168693495873	26.91406656802699	20.270846473828065
20-21	21.988852002896298	32.284612384327374	25.974479565280184	19.752056047496147
22-23	21.38952885753725	32.710309526499614	25.998482087154724	19.901679528808415
24-25	20.73016233252673	32.47254749967824	26.404383164889882	20.392907002905147
26-27	20.43625588622214	32.16957330354485	26.639521026327245	20.75464978390577
28-29	20.517567702006772	32.20548444901885	26.672594827025044	20.604353021949343
30-31	20.974480522670337	32.96551554747752	25.951147025593162	20.108856904258975
32-33	20.085056632784017	32.336674931626796	26.874555589532363	20.703712846056828
34-35	20.40438982192445	31.595429405676352	26.931378699018378	21.068802073380823
36-37	21.17193126207831	32.29345661436949	26.089196993231727	20.445415130320473
38-39	20.80218814758305	32.14517207763738	26.533843855254315	20.518795919525253
40-41	20.3794136053847	31.88792126911019	26.900752274163015	20.831912851342096
42-43	20.248183253684765	31.915886002622667	26.955659526745784	20.880271216946785
44-45	20.808685065194236	32.05531986278104	26.540256306784144	20.595738765240583
46-47	20.561397975915735	32.63032707401829	26.24808049964484	20.560194450421136
48-49	20.364845932871454	31.88090204230804	26.8053667076674	20.948885317153106
50-51	20.41720230659612	31.618335857411132	26.787580063535042	21.1768817724577
52-53	20.46778052807307	31.799526560598817	26.544142894405525	21.188550016922587
54-55	20.69407199744954	32.362673287121794	26.353667097146893	20.589587618281772
56-57	20.304807031522092	32.03219751766796	26.246508038572575	21.416487412237377
58-59	20.269900528060912	31.292619427729342	26.842392238732653	21.5950878054771
60-61	20.901213248197763	31.995322570464158	26.155118044675298	20.948346136662778
62-63	20.506621670743808	31.95059290479138	26.349853124662285	21.19293229980253
64-65	20.52734827371337	31.542896384977553	26.722719085961756	21.20703625534733
66-67	20.465152492384835	31.5600314578328	26.237178501587383	21.737637548194982
68-69	21.12910598115815	31.881686929214005	26.206192408685908	20.783014680941942
70-71	21.19406929569079	31.462005166745183	26.35283200807973	20.991093529484292
72-73	20.73915137564543	31.513687683700407	26.899843227581332	20.847317713072826
74-75	20.65057104261329	31.55463588358099	26.64993245732531	21.14486061648041
76-77	20.759670882966965	31.699349134225713	26.09895615866388	21.442023824143437
78-79	21.098594489711957	31.739431041575845	26.38085706040194	20.78111740831026
80-81	20.632835656664994	31.671501960479947	26.509264999317196	21.186397383537862
82-83	20.480536328568054	31.68250590563655	26.662211343439594	21.1747464223558
84-85	20.6896136728088	31.528256068959404	26.470190202768173	21.31194005546363
86-87	21.099376177235516	31.673588342798702	26.209078746970714	21.017956732995064
88-89	20.639499470469072	31.512935684096977	26.51810327719716	21.32946156823679
90-91	20.505787282077378	31.332767122991594	26.369306356308737	21.79213923862229
92-93	20.79441857632041	31.655362905028262	26.215950559817426	21.334267958833905
94-95	20.690910314483975	31.434496197618238	26.500376885625464	21.374216602272316
96-97	20.615533717330674	31.611256237173208	26.398089575582407	21.375120469913714
98-99	20.44048721649834	31.531728681330907	26.44365014980829	21.58413395236246
100	20.734184975198378	31.830270258587234	26.095736518263013	21.33980824795137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37807.0
1	27559.0
2	15059.0
3	10457.0
4	6603.0
5	4383.0
6	3471.0
7	3451.5
8	3766.0
9	3907.5
10	3195.5
11	2265.5
12	2026.5
13	1932.5
14	1905.0
15	1967.0
16	2091.5
17	2267.0
18	2491.5
19	2820.0
20	3397.5
21	4228.0
22	5452.5
23	7515.5
24	9915.0
25	12466.5
26	16082.5
27	20713.5
28	26534.0
29	31717.0
30	35224.0
31	39173.0
32	43440.0
33	46802.0
34	50381.5
35	54364.0
36	57187.5
37	59364.0
38	61477.0
39	62152.5
40	62782.0
41	63030.5
42	61107.0
43	60634.5
44	60753.0
45	59722.5
46	59855.0
47	59944.0
48	60199.5
49	62185.5
50	63577.5
51	61382.0
52	57274.5
53	53781.0
54	51455.5
55	50040.5
56	46359.0
57	42023.5
58	39509.0
59	36484.5
60	31882.5
61	26926.5
62	22246.5
63	17520.0
64	13472.0
65	10397.5
66	7897.0
67	5925.0
68	4447.0
69	3276.5
70	2434.0
71	1927.5
72	1522.5
73	1194.0
74	988.5
75	786.0
76	560.5
77	473.0
78	390.0
79	265.0
80	181.0
81	130.0
82	110.0
83	97.5
84	85.0
85	77.5
86	56.5
87	43.5
88	36.0
89	23.5
90	27.5
91	25.0
92	21.0
93	19.0
94	16.0
95	15.5
96	13.5
97	11.0
98	13.5
99	16.0
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013606404539528815
2	0.007613692070855474
3	0.009480274643065204
4	0.008596103950965857
5	0.010560927711186626
6	0.01159246018530253
7	0.01031532474115903
8	0.007318968506822359
9	0.006483918408728533
10-11	0.005722549201642986
12-13	0.006385677220717495
14-15	0.007417209694833397
16-17	0.004617335836518803
18-19	0.005599747716629188
20-21	0.005108541776573996
22-23	0.005697988904640227
24-25	0.005305024152596072
26-27	0.0058944712806623025
28-29	0.015890512160785457
30-31	0.005206782964585034
32-33	0.005083981479571236
34-35	0.004764697618535361
36-37	0.006483918408728533
38-39	0.00547694623161539
40-41	0.005501506528618149
42-43	0.004543654945510525
44-45	0.004003328411449814
46-47	0.0058944712806623025
48-49	0.0034384415803863436
50-51	0.0038805269264360165
52-53	0.0031928386103587474
54-55	0.004666456430524323
56-57	0.0028981150463256323
58-59	0.0027507532643090745
60-61	0.003561243065400141
62-63	0.0032419592043642664
64-65	0.004543654945510525
66-67	0.0031928386103587474
68-69	0.003512122471394622
70-71	0.004666456430524323
72-73	0.0025788311852897577
74-75	0.0027507532643090745
76-77	0.0027507532643090745
78-79	0.0033647606893780642
80-81	0.003929647520441535
82-83	0.0019157031662152483
84-85	0.0028244341553173534
86-87	0.0025297105912842383
88-89	0.002063064948231806
90-91	0.0024069091062704404
92-93	0.002603391482292517
94-95	0.0020876252452345654
96-97	0.0013753766321545373
98-99	0.0018420222752069696
100	0.002554270888286998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2035806.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.83000933669798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.36333564304068	57.37899170648778
2	11.5076926763768	15.841491887177323
3	2.7590994292562034	5.697265184297477
4	0.8990075633898774	2.4751479592754944
5	0.4092836726853069	1.4085499506143855
6	0.22465421234396238	0.9277770919898075
7	0.14706490965027502	0.7085735373030352
8	0.10304713432402922	0.5674188174114322
9	0.07845959069223644	0.4860336923910123
>10	0.43939212303640235	5.735566026011035
>50	0.040580338646175475	1.9691776540508885
>100	0.026810817231540635	3.2406156878991865
>500	7.859446632399475E-4	0.3514310454771253
>1k	6.430456335599466E-4	0.7552865934001707
>5k	7.144951483999407E-5	0.27289264922369755
>10k+	7.144951483999407E-5	2.1837805169901405
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44405	2.1811999768150794	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5549	0.272570176136626	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3368	0.16543816061058864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	2.7016326703035553E-4	0.0
22-23	0.0	0.0	0.0	0.00120345455313522	0.0
24-25	0.0	0.0	0.0	0.0027998738583145935	0.0
26-27	0.0	0.0	0.0	0.005182222667582274	0.0
28-29	0.0	0.0	0.0	0.010020601177125915	0.0
30-31	0.0	0.0	0.0	0.020949933343353933	0.0
32-33	0.0	0.0	0.0	0.0385351059973298	0.0
34-35	0.0	0.0	0.0	0.06358660894014459	0.0
36-37	0.0	0.0	0.0	0.09949376315817912	0.0
38-39	0.0	0.0	0.0	0.14998973379585284	0.0
40-41	0.0	0.0	0.0	0.20647841690219992	0.0
42-43	0.0	0.0	0.0	0.2634583059486022	0.0
44-45	0.0	0.0	0.0	0.3337253156734974	0.0
46-47	0.0	0.0	0.0	0.41700928280985516	0.0
48-49	0.0	0.0	0.0	0.5116401071614879	0.0
50-51	0.0	0.0	0.0	0.6075235066602613	0.0
52-53	0.0	0.0	0.0	0.702670097248952	0.0
54-55	0.0	0.0	0.0	0.8119634189112321	0.0
56-57	0.0	0.0	0.0	0.9351824289740771	0.0
58-59	0.0	0.0	0.0	1.0681027563530119	0.0
60-61	0.0	0.0	0.0	1.209299903821877	0.0
62-63	0.0	0.0	0.0	1.3521917117839322	0.0
64-65	0.0	0.0	0.0	1.5162053751683608	0.0
66-67	0.0	0.0	0.0	1.7079967344629106	0.0
68-69	4.912059400551919E-5	0.0	0.0	1.914303229286091	0.0
70-71	4.912059400551919E-5	0.0	0.0	2.1418543810166586	0.0
72-73	4.912059400551919E-5	0.0	0.0	2.396544660935276	0.0
74-75	4.912059400551919E-5	0.0	0.0	2.6469860094724154	0.0
76-77	4.912059400551919E-5	0.0	0.0	2.9032972689932146	0.0
78-79	4.912059400551919E-5	0.0	0.0	3.1935999795658327	0.0
80-81	4.912059400551919E-5	0.0	0.0	3.508610348923227	0.0
82-83	4.912059400551919E-5	0.0	0.0	3.842728629348769	0.0
84-85	4.912059400551919E-5	0.0	0.0	4.186695588872417	0.0
86-87	4.912059400551919E-5	0.0	0.0	4.5524475318375135	0.0
88	4.912059400551919E-5	0.0	0.0	4.843978257260269	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	2215	0.0	43.292564	2
GGTATCA	3995	0.0	42.832508	1
GTACCTG	2435	0.0	39.9632	1
GTCTCGC	580	0.0	38.09419	1
TCTGTCG	685	0.0	34.9923	8
GTATCAA	6225	0.0	32.09508	1
TATAACG	145	2.6588168E-8	29.17638	2
CGCTCTG	635	0.0	28.869356	5
TCTCGCT	735	0.0	28.779419	2
ACCTGGG	3220	0.0	28.758566	3
TCGCTCT	740	0.0	28.58426	4
CTGTCGC	925	0.0	26.421318	9
ATCAACG	7395	0.0	26.379513	3
TCAACGC	7350	0.0	26.156654	4
CTCGCTC	785	0.0	25.74872	3
GTACATG	11520	0.0	25.6269	1
TATCAAC	7910	0.0	25.375128	2
CATGGGT	4615	0.0	25.361435	4
TACATGG	11450	0.0	25.330078	2
CAACGCA	7915	0.0	24.408278	5
>>END_MODULE