FastQCFastQC Report
Wed 25 May 2016
SRR2431462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035806
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283811.39409157847064No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251581.2357759039908518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237451.1663685046610532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184000.9038189297015531No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119990.5893980074722247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84260.4138901250905047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65530.32188725251816724No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54240.2664301018859361No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.11071781888844026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84700.057.0004121
TATAACG2450.053.757022
TACCTGG21800.049.6266062
ATAACGC3050.046.2662853
GTACCTG25450.043.0806431
GTATCAA159700.042.7539831
ACCTGGG29550.035.6560943
GTGGTAT31400.034.0181161
TGGTATC32250.033.1084862
ATCAACG212050.031.920213
TATCAAC213500.031.8356112
TCAACGC215050.031.4967884
GTATAAC5300.031.074651
CAACGCA222300.030.4530945
TCTGTCG5500.029.915978
AACGCAG230450.029.416186
TAACGCA5450.028.4813584
GCGGTAT2350.028.03331
CGGTATC2203.6379788E-1227.7948192
CATGGGT40500.026.4803094
Produced by FastQC (version 0.11.5)