##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431462_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2035806
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20020620825363	33.0	31.0	34.0	30.0	34.0
2	32.318414426521976	33.0	31.0	34.0	30.0	34.0
3	32.421026364987625	34.0	31.0	34.0	30.0	34.0
4	35.98710682648543	37.0	35.0	37.0	35.0	37.0
5	35.93326672580786	37.0	35.0	37.0	35.0	37.0
6	35.98984431718936	37.0	35.0	37.0	35.0	37.0
7	35.92823333854012	37.0	35.0	37.0	35.0	37.0
8	35.91327267922386	37.0	35.0	37.0	35.0	37.0
9	37.58389895697331	39.0	37.0	39.0	35.0	39.0
10-11	37.51723248678901	39.0	37.0	39.0	34.5	39.0
12-13	37.50006926003755	39.0	37.0	39.0	35.0	39.0
14-15	38.770222948552075	40.0	38.0	41.0	34.0	41.0
16-17	38.8218582713677	40.0	38.0	41.0	34.5	41.0
18-19	38.93452151138173	40.0	38.5	41.0	35.0	41.0
20-21	38.91206111977271	40.0	38.5	41.0	35.0	41.0
22-23	38.76483491059561	40.0	38.0	41.0	35.0	41.0
24-25	38.6609058034017	40.0	38.0	41.0	34.0	41.0
26-27	38.46155503029267	40.0	38.0	41.0	34.0	41.0
28-29	38.323900214460515	40.0	38.0	41.0	34.0	41.0
30-31	38.019013353924684	40.0	37.0	41.0	34.0	41.0
32-33	37.64750963500451	40.0	36.0	41.0	33.0	41.0
34-35	37.37398209849072	40.0	35.0	41.0	33.0	41.0
36-37	37.14961961012003	40.0	35.0	41.0	32.5	41.0
38-39	37.00512990923497	40.0	35.0	41.0	32.0	41.0
40-41	36.8377912728423	40.0	35.0	41.0	31.0	41.0
42-43	36.63803353561194	40.0	35.0	41.0	31.0	41.0
44-45	36.318616557766305	40.0	35.0	41.0	30.0	41.0
46-47	35.83222590954148	40.0	35.0	41.0	28.0	41.0
48-49	35.43793858550373	40.0	35.0	41.0	25.0	41.0
50-51	35.25900355927824	40.0	35.0	41.0	24.0	41.0
52-53	35.11290786057218	39.0	35.0	41.0	24.0	41.0
54-55	34.94420170684241	39.0	35.0	41.0	24.0	41.0
56-57	34.716342323384445	39.0	35.0	41.0	23.0	41.0
58-59	34.54059718853368	39.0	35.0	41.0	23.0	41.0
60-61	34.308844506794856	38.0	35.0	41.0	22.0	41.0
62-63	34.04876962736135	37.5	34.0	40.5	22.0	41.0
64-65	33.78992767483739	37.0	34.0	40.0	21.5	41.0
66-67	33.51764362616084	36.5	34.0	40.0	20.0	41.0
68-69	33.208687124411654	36.0	34.0	39.5	19.5	41.0
70-71	32.86674810861153	35.5	33.5	39.0	18.0	41.0
72-73	32.540553471205016	35.0	33.0	39.0	16.0	41.0
74-75	32.242117127073996	35.0	33.0	37.5	13.5	40.0
76-77	31.902979949955938	35.0	33.0	37.0	10.5	39.5
78-79	31.606541340383124	35.0	33.0	37.0	7.5	39.0
80-81	31.320252273546693	35.0	33.0	36.0	6.0	39.0
82-83	31.079865419396544	35.0	33.0	36.0	2.0	38.0
84-85	30.85734053244759	35.0	33.0	36.0	2.0	37.0
86-87	30.621546945042898	35.0	32.5	35.0	2.0	37.0
88-89	30.46786064094516	35.0	33.0	35.0	2.0	36.0
90-91	30.281460758048656	35.0	32.0	35.0	2.0	36.0
92-93	30.086019493016526	35.0	32.0	35.0	2.0	36.0
94-95	29.913279801709987	35.0	32.0	35.0	2.0	36.0
96-97	29.733376362973683	35.0	32.0	35.0	2.0	35.5
98-99	29.5684257242586	35.0	32.0	35.0	2.0	35.0
100	29.450375919905923	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18380797905352608
1101	2	0.2732589526872218
1101	3	0.2575444965317928
1101	4	0.15579664829616036
1101	5	0.12683967420648656
1101	6	0.12732905243083792
1101	7	0.22483738377027152
1101	8	0.2535795129436451
1101	9	0.3298251954645508
1101	10-11	0.30811082079748786
1101	12-13	0.3717374332041743
1101	14-15	0.44721363429860617
1101	16-17	0.39266867494663416
1101	18-19	0.3796829424981212
1101	20-21	0.333287597432097
1101	22-23	0.30462915200694596
1101	24-25	0.3225035931814162
1101	26-27	0.300732152074211
1101	28-29	0.2494597615850438
1101	30-31	0.27328310550434765
1101	32-33	0.3952844966765596
1101	34-35	0.4537059044670855
1101	36-37	0.43786466194253393
1101	38-39	0.40863116943411626
1101	40-41	0.48939182960872074
1101	42-43	0.5736259688164509
1101	44-45	0.6757671635205114
1101	46-47	0.633092231238173
1101	48-49	0.6012687112711603
1101	50-51	0.5515593520394404
1101	52-53	0.4770188449831565
1101	54-55	0.4668043410832894
1101	56-57	0.4617880381101003
1101	58-59	0.42364810414836995
1101	60-61	0.4706830633025021
1101	62-63	0.42677272247804154
1101	64-65	0.489108332857505
1101	66-67	0.3674091232005807
1101	68-69	0.3555334315463412
1101	70-71	0.36902224774456727
1101	72-73	0.36666892341317237
1101	74-75	0.3512385830372722
1101	76-77	0.366308472223416
1101	78-79	0.30252665805580037
1101	80-81	0.2508692097484122
1101	82-83	0.18100351195997177
1101	84-85	0.27019088293833704
1101	86-87	0.24223105644952625
1101	88-89	0.2440472426847542
1101	90-91	0.2705962837084641
1101	92-93	0.34309084342642393
1101	94-95	0.397720053255572
1101	96-97	0.367439999057698
1101	98-99	0.46804209495244464
1101	100	0.5160430261617357
1102	1	0.19754151890649752
1102	2	0.29571146371957724
1102	3	0.2768325140392278
1102	4	0.1865316109380828
1102	5	0.21343444129298206
1102	6	0.1624508362881727
1102	7	0.1495634534649355
1102	8	0.2614758640263801
1102	9	0.3499335247269002
1102	10-11	0.3693854482220189
1102	12-13	0.3050293945742055
1102	14-15	0.46366518578975047
1102	16-17	0.3648370594852608
1102	18-19	0.3376977297352255
1102	20-21	0.33356399098103395
1102	22-23	0.3473739306898267
1102	24-25	0.3846056396235511
1102	26-27	0.3472008326758882
1102	28-29	0.26051331669251
1102	30-31	0.3403373346476002
1102	32-33	0.4633676387718779
1102	34-35	0.5247154771602283
1102	36-37	0.5008347663658412
1102	38-39	0.3974799350240801
1102	40-41	0.5084848529860224
1102	42-43	0.532194636576456
1102	44-45	0.6040002725032778
1102	46-47	0.6739539497840141
1102	48-49	0.566862088128488
1102	50-51	0.3427407455416045
1102	52-53	0.4621025837240751
1102	54-55	0.40271141064710037
1102	56-57	0.560324707394102
1102	58-59	0.5557130118019415
1102	60-61	0.5915468034697255
1102	62-63	0.559897887934504
1102	64-65	0.5178355241632673
1102	66-67	0.5392183905122536
1102	68-69	0.4193742060217147
1102	70-71	0.42860060847447556
1102	72-73	0.25585582461869905
1102	74-75	0.32026525373908044
1102	76-77	0.36955576232134746
1102	78-79	0.2884309515349628
1102	80-81	0.13550491150920152
1102	82-83	0.19251855317541455
1102	84-85	0.18222295625321294
1102	86-87	0.1780957254205653
1102	88-89	0.24433238363392462
1102	90-91	0.20719506609464844
1102	92-93	0.22191140715506208
1102	94-95	0.19216975491455557
1102	96-97	0.21854545783636325
1102	98-99	0.34017180869255625
1102	100	0.3288589880289017
1103	1	0.0619189992693876
1103	2	0.20387914820874187
1103	3	0.1305829426017695
1103	4	0.10925146632457228
1103	5	0.10635823162466096
1103	6	0.14447264523484193
1103	7	0.1403859852901732
1103	8	0.12138389329535926
1103	9	0.2728674989917721
1103	10-11	0.22093038147610145
1103	12-13	0.21134783835866244
1103	14-15	0.30000578904754605
1103	16-17	0.2572768679285886
1103	18-19	0.25593520186168917
1103	20-21	0.21209126881675644
1103	22-23	0.2694713886747522
1103	24-25	0.2911027716942627
1103	26-27	0.2891189906063403
1103	28-29	0.22245672718698017
1103	30-31	0.209316126958079
1103	32-33	0.2514869699509532
1103	34-35	0.33367419685924915
1103	36-37	0.2649059374496616
1103	38-39	0.2462971850928426
1103	40-41	0.13933758821001874
1103	42-43	0.11723744677041026
1103	44-45	0.1399081146987342
1103	46-47	0.07891073686233341
1103	48-49	-0.04097481635264444
1103	50-51	0.004537615107061299
1103	52-53	-0.035284764413034964
1103	54-55	-0.010362525085817254
1103	56-57	0.06642254850183349
1103	58-59	-0.006114316262539887
1103	60-61	-0.053104907314121874
1103	62-63	-0.06332091286041219
1103	64-65	-0.07697750801160197
1103	66-67	-0.15767643696378997
1103	68-69	-0.12466289028063926
1103	70-71	-0.21992650047541673
1103	72-73	-0.25164949676099013
1103	74-75	-0.27143921638519686
1103	76-77	-0.35890778178424654
1103	78-79	-0.3037169324496922
1103	80-81	-0.3004364007856033
1103	82-83	-0.2734121571052093
1103	84-85	-0.2590526088381573
1103	86-87	-0.3672939927869443
1103	88-89	-0.39430250399618316
1103	90-91	-0.2679801942818223
1103	92-93	-0.2224029807714203
1103	94-95	-0.23599342044124683
1103	96-97	-0.22930696996819222
1103	98-99	-0.15205731763982655
1103	100	-0.12225134177203856
1104	1	0.09726591641876325
1104	2	0.14486412446667885
1104	3	0.15198159725628102
1104	4	0.13643474480394957
1104	5	0.08737614315644748
1104	6	0.1725900133254541
1104	7	0.16376203861673844
1104	8	0.12189461443222882
1104	9	0.14081374287307824
1104	10-11	0.14738436253649212
1104	12-13	0.15992196356124566
1104	14-15	0.25816649688117366
1104	16-17	0.19844576561812488
1104	18-19	0.32024862825213063
1104	20-21	0.2472424724975184
1104	22-23	0.2885202796050521
1104	24-25	0.2648311436100812
1104	26-27	0.2691915356759651
1104	28-29	0.19315941123777947
1104	30-31	0.2397839456876696
1104	32-33	0.2376530787664919
1104	34-35	0.2678537366904621
1104	36-37	0.29288569361323624
1104	38-39	0.393986552613768
1104	40-41	0.3890217791411885
1104	42-43	0.3095947070245515
1104	44-45	0.35065013377938925
1104	46-47	0.4214726748229438
1104	48-49	0.2771473235059432
1104	50-51	0.22001722816339253
1104	52-53	0.2334962297541452
1104	54-55	0.3199202388028013
1104	56-57	0.33207287132887586
1104	58-59	0.28666942647962657
1104	60-61	0.38894055087405377
1104	62-63	0.36441101590011016
1104	64-65	0.364048740375857
1104	66-67	0.39819629512982857
1104	68-69	0.4383607391297488
1104	70-71	0.38909437349082765
1104	72-73	0.4415267376363232
1104	74-75	0.3808492745408927
1104	76-77	0.4538810372581281
1104	78-79	0.4131126971154835
1104	80-81	0.3847097426229702
1104	82-83	0.39059309641900697
1104	84-85	0.33887928539345324
1104	86-87	0.31429255824601654
1104	88-89	0.30575472745334054
1104	90-91	0.43047354910605407
1104	92-93	0.3889274724045748
1104	94-95	0.3528863680989076
1104	96-97	0.32837406527648483
1104	98-99	0.46347384837052985
1104	100	0.560683565725725
1105	1	0.13574481065515442
1105	2	0.1778018938420658
1105	3	0.19093879919115864
1105	4	0.09267875504741596
1105	5	0.09461491121736287
1105	6	0.14074763840553572
1105	7	0.20617693388655312
1105	8	0.18639283648919758
1105	9	0.27479095866840453
1105	10-11	0.24589872835110071
1105	12-13	0.18078966435010813
1105	14-15	0.25752628028109825
1105	16-17	0.3139557655657583
1105	18-19	0.281666522546125
1105	20-21	0.2080681555261492
1105	22-23	0.2728458832066778
1105	24-25	0.22561820416488132
1105	26-27	0.25869276897665117
1105	28-29	0.32910310010735344
1105	30-31	0.370979470543098
1105	32-33	0.378490693302858
1105	34-35	0.4316071341589236
1105	36-37	0.4158373932639279
1105	38-39	0.37789778289448606
1105	40-41	0.31085731111885195
1105	42-43	0.41288298516921174
1105	44-45	0.3978932746898849
1105	46-47	0.3369202241942446
1105	48-49	0.30575173860022176
1105	50-51	0.22141243775468666
1105	52-53	0.29409838248791687
1105	54-55	0.26503792169876306
1105	56-57	0.2619218029046948
1105	58-59	0.1831491952859352
1105	60-61	0.27062922144050106
1105	62-63	0.20265398573705085
1105	64-65	0.2465825230391303
1105	66-67	0.2919393234459946
1105	68-69	0.24663179645161648
1105	70-71	0.2674222342160064
1105	72-73	0.3734130592580982
1105	74-75	0.39725357023280594
1105	76-77	0.38525282897433044
1105	78-79	0.344403970793028
1105	80-81	0.33028107593186107
1105	82-83	0.3161254755808507
1105	84-85	0.388633105308859
1105	86-87	0.3798949030011265
1105	88-89	0.29059769763940935
1105	90-91	0.21517485991353524
1105	92-93	0.2177155733659042
1105	94-95	0.33985117407879883
1105	96-97	0.2532134219700879
1105	98-99	0.2209873378695839
1105	100	0.29048466653169314
1106	1	0.04580699790570719
1106	2	0.10390565179785227
1106	3	0.1219619325769159
1106	4	0.1466680362082684
1106	5	0.10468990746373663
1106	6	0.07057024653763477
1106	7	0.011834565545640885
1106	8	0.11473095835109604
1106	9	0.10391980274560098
1106	10-11	0.1067601630095325
1106	12-13	0.06502338102021099
1106	14-15	0.14262704296773165
1106	16-17	0.19986483102650965
1106	18-19	0.1454906736956758
1106	20-21	0.19791935910520664
1106	22-23	0.09402975676577086
1106	24-25	0.19294063203793854
1106	26-27	0.19756872869702846
1106	28-29	0.1050694158414629
1106	30-31	0.22021642339326064
1106	32-33	0.13732932455562263
1106	34-35	0.30866685885882816
1106	36-37	0.30080327976943977
1106	38-39	0.27461836186110133
1106	40-41	0.2889531860284009
1106	42-43	0.23699394754893177
1106	44-45	0.16769621549959624
1106	46-47	0.2684304811460976
1106	48-49	0.19361226729787973
1106	50-51	0.2520527645452475
1106	52-53	0.27446647570347693
1106	54-55	0.22213192106594448
1106	56-57	0.2885227365193117
1106	58-59	0.30537606889846813
1106	60-61	0.22360430158155253
1106	62-63	0.23294715480985673
1106	64-65	0.27291815775031836
1106	66-67	0.34814929003233175
1106	68-69	0.3010653631461935
1106	70-71	0.2526362207911603
1106	72-73	0.3306805314494312
1106	74-75	0.29496451361110587
1106	76-77	0.24209798602873178
1106	78-79	0.22196534581752303
1106	80-81	0.10420781356545561
1106	82-83	0.12644227343369252
1106	84-85	0.15869296827805357
1106	86-87	0.17605829064969214
1106	88-89	0.13719799080424266
1106	90-91	0.23345408160162862
1106	92-93	0.29972495701568747
1106	94-95	0.3648244745328846
1106	96-97	0.259029002968326
1106	98-99	0.3117750190087172
1106	100	0.3645316899670661
1107	1	0.022654569225672105
1107	2	0.14002710073929592
1107	3	0.05514376735828108
1107	4	0.08808158741621241
1107	5	0.018753004010029883
1107	6	0.0395782657798307
1107	7	0.013385170931201174
1107	8	-0.005064875494056764
1107	9	0.02803226635109013
1107	10-11	0.09572582020228282
1107	12-13	-0.025917871515375168
1107	14-15	0.02958317948906597
1107	16-17	0.015336150325474307
1107	18-19	0.039158111688053054
1107	20-21	0.014203226335375518
1107	22-23	0.06501374484064826
1107	24-25	0.08169377350261442
1107	26-27	0.010149444004795782
1107	28-29	0.030080018803481323
1107	30-31	-0.022857518468477167
1107	32-33	-0.010528980862801518
1107	34-35	-0.020124457419029795
1107	36-37	-0.05618703937349068
1107	38-39	-0.09722990249004226
1107	40-41	-0.10776784277845053
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2216	64-65	-0.8650633230937146
2216	66-67	-0.7421532092612111
2216	68-69	-0.6388338357766372
2216	70-71	-0.8399396461885615
2216	72-73	-0.6739177371928093
2216	74-75	-0.6889649112255718
2216	76-77	-0.6679175199819483
2216	78-79	-0.5885968531381245
2216	80-81	-0.4830828412813126
2216	82-83	-0.5602582604309738
2216	84-85	-0.5539820347038713
2216	86-87	-0.6585883572261757
2216	88-89	-0.5917260025258528
2216	90-91	-0.5912370389858239
2216	92-93	-0.5644657645141322
2216	94-95	-0.7336326894121576
2216	96-97	-1.0194452800287763
2216	98-99	-1.0155686140407774
2216	100	-1.0671109785397235
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	236.0
3	598.0
4	388.0
5	390.0
6	720.0
7	1600.0
8	2600.0
9	3711.0
10	4869.0
11	6423.0
12	8963.0
13	12779.0
14	11987.0
15	13865.0
16	17515.0
17	25337.0
18	28234.0
19	15473.0
20	9926.0
21	9031.0
22	8845.0
23	9323.0
24	10131.0
25	11989.0
26	14057.0
27	17579.0
28	21215.0
29	26699.0
30	32737.0
31	40452.0
32	50866.0
33	64204.0
34	89334.0
35	147169.0
36	199737.0
37	368311.0
38	518330.0
39	228769.0
40	1414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.346958672861135	20.396582886717702	14.010085830793528	22.246372609627635
2	14.955747256860427	22.344270524794602	39.53323646752195	23.166745750823015
3	16.581303219119707	29.079258573389886	31.25368097632141	23.085757231168998
4	10.983384634744114	18.872365170356403	37.56742833941708	32.5768218554824
5	10.676624212989184	37.64958547611668	34.76700255291891	16.90678775797523
6	26.190413035426758	38.71773636584233	19.440899574910382	15.650951023820541
7	23.398398472153044	34.46187898061014	24.01672850949452	18.1229940377423
8	25.14311285063508	32.19570037616551	22.6468533838686	20.01433338933081
9	25.279275137218377	15.254793433166029	24.2034358873095	35.26249554230609
10-11	22.468518754141947	26.699014579158636	29.65918262978998	21.173284036909436
12-13	24.803468503383918	25.351752573673526	30.119446548443218	19.725332374499338
14-15	22.097758823777905	26.02146275234477	30.524666888691755	21.356111535185573
16-17	18.552013305786506	29.033684938545225	30.81354510203821	21.60075665363006
18-19	18.175749555704225	25.444909780204988	36.60167029667856	19.77767036741222
20-21	20.168252770647104	24.656868090574445	36.586713075803885	18.58816606297457
22-23	19.5015880688042	25.095195711182694	36.738078186231895	18.665138033781215
24-25	19.07870434955172	24.910943925619268	36.92730018135412	19.083051543474884
26-27	18.840400313192905	24.734896154152214	37.055470904398554	19.369232628256327
28-29	18.945529753789607	24.777415457661718	37.01378745794956	19.26326733059911
30-31	19.506844362442596	25.463587569351777	36.368220387168904	18.661347681036723
32-33	18.604381569898813	24.968931132724236	37.095220552117105	19.33146674525985
34-35	18.92662367725274	24.59842639587675	36.704003025878386	19.77094690099213
36-37	19.834373177856886	25.25527810539853	35.612026402439234	19.29832231430535
38-39	19.576583648283158	25.190222490315723	35.8431977223405	19.38999613906062
40-41	19.13185972726875	25.0542176279622	36.01460277820213	19.79931986656692
42-43	19.142523186018416	25.214913225431147	35.78401911616943	19.858544472381002
44-45	19.774330167019844	25.425801869136844	35.01387165574716	19.785996308096156
46-47	19.707897969123124	26.2248590293082	34.06911450585287	19.998128495715804
48-49	19.844266997807242	25.761841091820774	33.90307064768896	20.490821262683024
50-51	19.941123029339504	25.6648035719154	33.48953135925685	20.904542039488245
52-53	19.980832979457343	26.12051792217119	33.01619986555578	20.882449232815688
54-55	20.200183860866986	26.76037692705763	32.75614218344481	20.28329702863057
56-57	19.764555275032244	26.228048177524997	32.88128116434832	21.12611538309444
58-59	19.685032987654807	25.4563251310418	33.50081793663816	21.357823944665235
60-61	20.299394211793775	26.17113117715467	32.7597686878846	20.76970592316695
62-63	19.96901386172853	25.9725781518252	33.10638983489615	20.952018151550117
64-65	19.87747229630641	25.727010889413886	33.45668463435103	20.93883217992867
66-67	19.93450247126194	25.855142101245605	32.892270466381305	21.31808496111115
68-69	20.630769121191364	26.118653521529417	32.594682786905985	20.65589457037324
70-71	20.715577416505038	25.757707118565143	32.60984091484586	20.91687455008396
72-73	20.295229506151372	25.856589478565244	32.9975203924146	20.85066062286878
74-75	20.23417786495059	25.921000214657315	32.68594751063831	21.158874409753793
76-77	20.31738289404786	26.099466255625536	32.23325307028273	21.349897780043875
78-79	20.535329213073624	26.24415829892415	32.367985169422845	20.852527318579384
80-81	20.117412955851393	26.024238065906086	32.507075821566495	21.35127315667603
82-83	19.90830557375084	26.044960085392184	32.67393119160265	21.37280314925433
84-85	20.10983918072281	25.981035905146616	32.341771234768544	21.56735367936204
86-87	20.3408625379825	26.117395272437548	32.08038978173755	21.461352407842398
88-89	19.823819165480405	25.998891839399235	32.340679809372794	21.83660918574756
90-91	19.665503491000617	25.851309014709656	32.1787046506396	22.30448284365013
92-93	19.867079444101282	26.196008699272156	32.04817264936064	21.88873920726593
94-95	19.745913903387653	25.847502168674225	32.3407043696698	22.06587955826832
96-97	19.66455889265973	26.078975457769122	32.166910551494084	22.089555098077067
98-99	19.572957145404416	25.8919505980722	32.201611069112694	22.33348118741069
100	19.825366035285374	26.09493757002772	31.82172520791571	22.257971186771204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6454.0
1	3854.5
2	9393.5
3	15549.5
4	8662.0
5	3698.0
6	5809.5
7	12518.0
8	19044.0
9	19579.5
10	12804.5
11	5863.0
12	4208.5
13	4168.0
14	4323.0
15	4540.0
16	4768.5
17	5132.5
18	5498.5
19	5774.0
20	6198.5
21	6832.0
22	7841.0
23	9637.0
24	11630.0
25	13868.5
26	17147.0
27	21423.0
28	27078.0
29	32255.0
30	35280.0
31	38481.0
32	42605.5
33	46363.5
34	49679.0
35	52990.0
36	55611.5
37	58087.0
38	60259.5
39	60754.0
40	61412.0
41	61791.5
42	60105.0
43	59794.5
44	60068.0
45	59159.0
46	59326.5
47	59678.0
48	60054.0
49	61751.5
50	63552.5
51	61228.0
52	56083.0
53	51987.0
54	48992.5
55	46801.0
56	43391.0
57	39226.0
58	36719.5
59	34511.0
60	30177.5
61	25105.0
62	20641.5
63	16071.5
64	12199.0
65	9292.5
66	6875.5
67	4900.5
68	3393.0
69	2400.5
70	1720.5
71	1252.5
72	974.0
73	744.0
74	602.0
75	495.0
76	315.0
77	223.5
78	177.0
79	100.5
80	38.0
81	9.5
82	8.0
83	6.5
84	3.0
85	1.0
86	1.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	2.0
95	2.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04283315797281273
2	0.0
3	4.9120594005519194E-5
4	0.06994772586385933
5	0.004764697618535361
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011248616027263894
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.912059400551919E-4
26-27	0.0
28-29	3.192838610358748E-4
30-31	0.003905087223438776
32-33	2.947235640331151E-4
34-35	0.001645539899184893
36-37	0.004175250490469131
38-39	0.0015718590081766142
40-41	0.0014244972261600567
42-43	0.0020876252452345654
44-45	0.0
46-47	5.157662370579515E-4
48-49	6.876883160772686E-4
50-51	0.0017437810871959313
52-53	8.596103950965859E-4
54-55	0.0013508163351517778
56-57	0.006213755141698177
58-59	0.009627636425081762
60-61	0.001399936929157297
62-63	0.002799873858314594
64-65	0.0010560927711186626
66-67	1.9648237602207678E-4
68-69	0.0010560927711186626
70-71	4.6664564305243234E-4
72-73	0.0
74-75	1.4736178201655756E-4
76-77	0.0
78-79	5.894471280662303E-4
80-81	0.0
82-83	0.0011052133651241818
84-85	5.648868310634707E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.7192207901931716E-4
94-95	0.0
96-97	4.420853460496727E-4
98-99	7.368089100827878E-4
100	2.4560297002759594E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2035806.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.643244635757156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.36160887171208	49.53750315001981
2	13.33096267026841	16.435275862270043
3	3.2895155496994226	6.083292352897461
4	1.1400747225826835	2.8111162010882937
5	0.5116842546264442	1.5770938842101476
6	0.28764851090723703	1.0638952516179645
7	0.18483220874444128	0.7975559942140649
8	0.13105919147790485	0.6463131041629622
9	0.09784588036488888	0.5428383785940486
>10	0.5717675128856592	6.707538903874431
>50	0.05319213045259972	2.2744922967307173
>100	0.036851958379079466	4.018744219079016
>500	0.001837847883324422	0.7736783787588755
>1k	4.794385782585425E-4	0.4215406866631713
>5k	2.3971928912927125E-4	1.0049888560398088
>10k+	3.9953214854878544E-4	5.304132479779186
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28381	1.39409157847064	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25158	1.2357759039908518	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23745	1.1663685046610532	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18400	0.9038189297015531	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11999	0.5893980074722247	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8426	0.4138901250905047	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6553	0.32188725251816724	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5424	0.2664301018859361	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2254	0.11071781888844026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	2.4560297002759594E-5	0.0
20-21	0.0	0.0	0.0	3.192838610358747E-4	0.0
22-23	0.0	0.0	0.0	0.0012771354441434989	0.0
24-25	0.0	0.0	0.0	0.0029472356403311513	0.0
26-27	0.0	0.0	0.0	0.005329584449598832	0.0
28-29	0.0	0.0	0.0	0.010241643850150752	0.0
30-31	0.0	0.0	0.0	0.021367458392400847	0.0
32-33	0.0	0.0	0.0	0.039443836986431904	0.0
34-35	0.0	0.0	0.0	0.06525670913633225	0.0
36-37	0.0	0.0	0.0	0.10202347374946336	0.0
38-39	0.0	0.0	0.0	0.15448426814735786	0.0
40-41	0.0	0.0	0.0	0.21320793828095602	0.0
42-43	2.4560297002759594E-5	0.0	0.0	0.27222633197858737	0.0
44-45	4.912059400551919E-5	0.0	0.0	0.34426168308768124	0.0
46-47	4.912059400551919E-5	0.0	0.0	0.4301736020033343	0.0
48-49	4.912059400551919E-5	0.0	0.0	0.5277025414012926	0.0
50-51	4.912059400551919E-5	0.0	0.0	0.6259191691153283	0.0
52-53	4.912059400551919E-5	0.0	0.0	0.7235954702953031	0.0
54-55	4.912059400551919E-5	0.0	0.0	0.8353939422518648	0.0
56-57	4.912059400551919E-5	0.0	0.0	0.9624443586471403	0.0
58-59	4.912059400551919E-5	0.0	0.0	1.099073290873492	0.0
60-61	4.912059400551919E-5	0.0	0.0	1.2450105756638894	0.0
62-63	4.912059400551919E-5	0.0	0.0	1.391856591443389	0.0
64-65	4.912059400551919E-5	0.0	0.0	1.5609787966043915	0.0
66-67	4.912059400551919E-5	0.0	0.0	1.7574857329234712	0.0
68-69	4.912059400551919E-5	0.0	0.0	1.9687042871472036	0.0
70-71	4.912059400551919E-5	0.0	0.0	2.202125349861431	0.0
72-73	4.912059400551919E-5	0.0	0.0	2.463520590861801	0.0
74-75	4.912059400551919E-5	0.0	0.0	2.7204949784016748	0.0
76-77	4.912059400551919E-5	0.0	0.0	2.982430545936106	0.0
78-79	4.912059400551919E-5	0.0	0.0	3.279929423530533	0.0
80-81	4.912059400551919E-5	0.0	0.0	3.6045674293130094	0.0
82-83	4.912059400551919E-5	0.0	0.0	3.948657190321671	0.0
84-85	4.912059400551919E-5	0.0	0.0	4.301957062706368	0.0
86-87	4.912059400551919E-5	0.0	0.0	4.680824204270937	0.0
88	4.912059400551919E-5	0.0	0.0	4.9822036087918	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8470	0.0	57.000412	1
TATAACG	245	0.0	53.75702	2
TACCTGG	2180	0.0	49.626606	2
ATAACGC	305	0.0	46.266285	3
GTACCTG	2545	0.0	43.080643	1
GTATCAA	15970	0.0	42.753983	1
ACCTGGG	2955	0.0	35.656094	3
GTGGTAT	3140	0.0	34.018116	1
TGGTATC	3225	0.0	33.108486	2
ATCAACG	21205	0.0	31.92021	3
TATCAAC	21350	0.0	31.835611	2
TCAACGC	21505	0.0	31.496788	4
GTATAAC	530	0.0	31.07465	1
CAACGCA	22230	0.0	30.453094	5
TCTGTCG	550	0.0	29.91597	8
AACGCAG	23045	0.0	29.41618	6
TAACGCA	545	0.0	28.481358	4
GCGGTAT	235	0.0	28.0333	1
CGGTATC	220	3.6379788E-12	27.794819	2
CATGGGT	4050	0.0	26.480309	4
>>END_MODULE