FastQCFastQC Report
Wed 25 May 2016
SRR2431461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6678745
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA448070.6708895159195328No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67380.100887217583543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134050.043.6571851
GTATCAA218650.036.590071
ATCAACG266500.029.1723863
TCAACGC268800.028.7129344
TATCAAC277650.028.4918162
GTACATG461000.025.807411
CAACGCA301850.025.6779235
TACCTGG52200.025.2128052
TACATGG459250.025.1779062
CATGGGT207350.024.618384
AACGCAG316550.024.4838376
ACATGGG475150.023.2472083
CGCAGAG334950.022.633368
ACGCAGA338700.022.6190077
GTACCTG65150.021.2843691
ATGGGTG117250.020.6855585
GCAGAGT349000.020.6178979
ATGGGTA85600.020.2620335
GTGGTAT72400.018.8283631
ACCTGGG68400.018.622883
Produced by FastQC (version 0.11.5)