##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431461_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6678745
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.751735243672275	31.0	30.0	34.0	25.0	34.0
2	29.787930067699843	31.0	30.0	34.0	24.0	34.0
3	29.799940857152055	31.0	30.0	34.0	23.0	34.0
4	33.01573109918106	37.0	35.0	37.0	24.0	37.0
5	33.246450044132544	37.0	35.0	37.0	27.0	37.0
6	33.15694670181299	37.0	35.0	37.0	26.0	37.0
7	33.074701609359245	37.0	35.0	37.0	25.0	37.0
8	32.967597804677375	37.0	35.0	37.0	25.0	37.0
9	34.18336453929593	39.0	35.0	39.0	19.0	39.0
10-11	34.16956022426369	39.0	35.0	39.0	20.0	39.0
12-13	34.14302836835364	39.0	35.0	39.0	19.5	39.0
14-15	35.27420555808015	40.0	36.0	41.0	19.5	41.0
16-17	35.3114180134142	40.0	36.0	41.0	21.5	41.0
18-19	35.32244575889632	40.0	36.0	41.0	22.0	41.0
20-21	35.4443019609223	40.0	36.0	41.0	23.5	41.0
22-23	35.42100125098353	40.0	35.5	41.0	24.0	41.0
24-25	35.47476793918618	40.0	35.0	41.0	25.0	41.0
26-27	35.49303746437392	40.0	35.0	41.0	25.0	41.0
28-29	35.39082028135525	40.0	35.0	41.0	25.0	41.0
30-31	35.3739505700547	40.0	35.0	41.0	25.0	41.0
32-33	35.315642160316045	40.0	35.0	41.0	25.0	41.0
34-35	35.2080119094231	40.0	35.0	41.0	25.0	41.0
36-37	35.07518987474443	40.0	35.0	41.0	25.0	41.0
38-39	34.96680723698839	40.0	35.0	41.0	24.0	41.0
40-41	34.87172155846645	40.0	34.0	41.0	24.0	41.0
42-43	34.63460964597391	39.0	34.0	41.0	23.0	41.0
44-45	34.491242740964054	39.0	34.0	41.0	23.0	41.0
46-47	34.34564577626485	39.0	33.0	41.0	22.0	41.0
48-49	34.20755351491934	39.0	33.0	41.0	21.5	41.0
50-51	34.02511497294776	39.0	33.0	40.5	20.0	41.0
52-53	33.9175081546009	38.0	33.0	40.0	20.0	41.0
54-55	33.83876634008335	38.0	33.0	40.0	19.0	41.0
56-57	33.67016924586879	38.0	33.0	40.0	18.0	41.0
58-59	33.45963403304064	38.0	33.0	40.0	16.5	41.0
60-61	33.24045370799454	37.5	32.5	40.0	13.5	41.0
62-63	32.970148508439834	37.0	32.0	40.0	11.5	41.0
64-65	32.825171869864775	37.0	32.0	40.0	8.0	41.0
66-67	32.57323531591639	36.0	32.0	40.0	7.5	41.0
68-69	32.31572421165953	36.0	32.0	39.0	4.0	41.0
70-71	32.02826406757557	35.0	31.0	39.0	2.0	41.0
72-73	31.724042540926476	35.0	31.0	39.0	2.0	41.0
74-75	31.39041167165388	35.0	31.0	38.0	2.0	40.0
76-77	31.07311403564592	35.0	31.0	37.0	2.0	39.5
78-79	30.74518079369702	35.0	31.0	37.0	2.0	39.0
80-81	30.45496414371263	35.0	30.5	36.0	2.0	39.0
82-83	30.16140524904005	35.0	30.0	36.0	2.0	38.0
84-85	29.879786097537785	35.0	30.0	35.5	2.0	37.0
86-87	29.632995195953733	35.0	30.0	35.0	2.0	37.0
88-89	29.381398226762663	34.5	30.0	35.0	2.0	36.5
90-91	29.16743834358102	34.0	30.0	35.0	2.0	36.0
92-93	28.989693048619166	34.0	29.0	35.0	2.0	36.0
94-95	28.770901793675307	34.0	29.0	35.0	2.0	36.0
96-97	28.694271528558133	34.0	29.0	35.0	2.0	35.0
98-99	28.551670935183182	34.0	29.0	35.0	2.0	35.0
100	28.418098759572345	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.388115284429297
1101	2	0.5390670354134919
1101	3	0.4571627492224515
1101	4	0.4387002738675676
1101	5	0.3539587654551326
1101	6	0.31698060717423004
1101	7	0.4076910701964209
1101	8	0.44961793813606477
1101	9	0.6465261484868776
1101	10-11	0.658337714766219
1101	12-13	0.5776660079760774
1101	14-15	0.6734183205305087
1101	16-17	0.6105855223736469
1101	18-19	0.5795097415425587
1101	20-21	0.5533696898422633
1101	22-23	0.5335843583913871
1101	24-25	0.47615970832249843
1101	26-27	0.5381291307534894
1101	28-29	0.6457466124829878
1101	30-31	0.5339800372684067
1101	32-33	0.5712906841645662
1101	34-35	0.5710033307998046
1101	36-37	0.5479900003974905
1101	38-39	0.5731723618169156
1101	40-41	0.5736332634752088
1101	42-43	0.584563807517192
1101	44-45	0.5292890708866551
1101	46-47	0.49442987781201
1101	48-49	0.44389988543948533
1101	50-51	0.45572434457938016
1101	52-53	0.3895619927635181
1101	54-55	0.48605529624893506
1101	56-57	0.4562256197892012
1101	58-59	0.5295710111249576
1101	60-61	0.5180267688467524
1101	62-63	0.5009478951108051
1101	64-65	0.5066313599659082
1101	66-67	0.554590850762473
1101	68-69	0.49578010812945195
1101	70-71	0.4953693319656054
1101	72-73	0.49057506240021453
1101	74-75	0.5876270490459135
1101	76-77	0.6167506773493159
1101	78-79	0.5577043413747944
1101	80-81	0.5110827682368573
1101	82-83	0.47314573041084174
1101	84-85	0.5092110923266198
1101	86-87	0.48955332406546503
1101	88-89	0.49469254603321744
1101	90-91	0.49387028484628814
1101	92-93	0.4414633413720743
1101	94-95	0.4467248648409097
1101	96-97	0.5477502547582205
1101	98-99	0.5447900229113891
1101	100	0.5313792137867495
1102	1	0.25105847176011054
1102	2	0.3611016424699969
1102	3	0.3127469165971668
1102	4	0.27859376885685094
1102	5	0.2471378338406467
1102	6	0.16961777725638427
1102	7	0.25507571323225875
1102	8	0.22821391301337002
1102	9	0.3277875538493902
1102	10-11	0.38421552447877616
1102	12-13	0.3788985080760696
1102	14-15	0.3843081241838959
1102	16-17	0.2885801723450072
1102	18-19	0.30353368505412703
1102	20-21	0.2903683001465396
1102	22-23	0.3053782903790321
1102	24-25	0.3409850825001115
1102	26-27	0.4173100471249853
1102	28-29	0.44610848681967497
1102	30-31	0.4131901648308798
1102	32-33	0.465927548789729
1102	34-35	0.44778640848250006
1102	36-37	0.3777679762255275
1102	38-39	0.26725891405848046
1102	40-41	0.39446708169728595
1102	42-43	0.35704712623118695
1102	44-45	0.2727546237222995
1102	46-47	0.24212394207442856
1102	48-49	0.25682280762932663
1102	50-51	0.1499145143699323
1102	52-53	0.18445318922954357
1102	54-55	0.22713640786198397
1102	56-57	0.24603492682955874
1102	58-59	0.2609764674061239
1102	60-61	0.19363598882846134
1102	62-63	0.22368067255012392
1102	64-65	0.20026639926160783
1102	66-67	0.23484440782903704
1102	68-69	0.27421627598684495
1102	70-71	0.2650011578076601
1102	72-73	0.22088011262158957
1102	74-75	0.2412571036460207
1102	76-77	0.27386217420617953
1102	78-79	0.2768501011186473
1102	80-81	0.27999765938565346
1102	82-83	0.24589813782288772
1102	84-85	0.16575541668454008
1102	86-87	0.22623825293225508
1102	88-89	0.24119116473218938
1102	90-91	0.25777629817654457
1102	92-93	0.22124495926790644
1102	94-95	0.28972924362528474
1102	96-97	0.33079458785134364
1102	98-99	0.3585232691703588
1102	100	0.337198303046641
1103	1	0.1883349456525849
1103	2	0.2686097953545925
1103	3	0.2221456790353109
1103	4	0.19523828927908937
1103	5	0.16464999385184598
1103	6	0.17190836600479287
1103	7	0.2224873549018369
1103	8	0.30951160339370887
1103	9	0.3166092930910551
1103	10-11	0.40093114303972754
1103	12-13	0.36038322341156004
1103	14-15	0.4286661570703103
1103	16-17	0.31232049111662263
1103	18-19	0.27102897345248067
1103	20-21	0.32756960263530743
1103	22-23	0.2454064628046737
1103	24-25	0.2706249243076968
1103	26-27	0.23344990253330877
1103	28-29	0.3729266410064298
1103	30-31	0.26556524063236964
1103	32-33	0.3924493637794555
1103	34-35	0.35465834291904486
1103	36-37	0.3440014756294545
1103	38-39	0.3963220354139665
1103	40-41	0.3448502563029052
1103	42-43	0.36808899394496564
1103	44-45	0.2963468139947878
1103	46-47	0.2820157682455928
1103	48-49	0.2247346604043159
1103	50-51	0.17622963360752664
1103	52-53	0.2299963575996884
1103	54-55	0.27682678218289425
1103	56-57	0.2254298207408567
1103	58-59	0.28371194420252976
1103	60-61	0.29897374526473897
1103	62-63	0.23590448896472793
1103	64-65	0.253364304453882
1103	66-67	0.26512937337251685
1103	68-69	0.18833288717756602
1103	70-71	0.23055941331868723
1103	72-73	0.2595244523835909
1103	74-75	0.19066097480069288
1103	76-77	0.205671620824738
1103	78-79	0.20645265630142973
1103	80-81	0.22745892710585736
1103	82-83	0.21584346160654988
1103	84-85	0.19477890938269127
1103	86-87	0.161899166987844
1103	88-89	0.2750225138315727
1103	90-91	0.28774995170164175
1103	92-93	0.23021718219303366
1103	94-95	0.24628935830799037
1103	96-97	0.23266169235163403
1103	98-99	0.22958955396432046
1103	100	0.25294238024934046
1104	1	0.08555469584365838
1104	2	0.20461771489020109
1104	3	0.12612011670470125
1104	4	0.17778283027545427
1104	5	0.08469747795178506
1104	6	0.07552334937853544
1104	7	0.20195975839056501
1104	8	0.25376923397559636
1104	9	0.22708714354316584
1104	10-11	0.3011510388337655
1104	12-13	0.22652099377229717
1104	14-15	0.2641833300644123
1104	16-17	0.2917855109763394
1104	18-19	0.24074248986409685
1104	20-21	0.21911200560541033
1104	22-23	0.24142822070978553
1104	24-25	0.22878687791002505
1104	26-27	0.35406150684676163
1104	28-29	0.4210612490069465
1104	30-31	0.3659338498975373
1104	32-33	0.42659912276757694
1104	34-35	0.37404391785235447
1104	36-37	0.3839715917149533
1104	38-39	0.3656603076423437
1104	40-41	0.3904395514652066
1104	42-43	0.3350500845940161
1104	44-45	0.2830253228742592
1104	46-47	0.27849416069137334
1104	48-49	0.271587067443015
1104	50-51	0.248916415073289
1104	52-53	0.1843342305155673
1104	54-55	0.18575644450330486
1104	56-57	0.2264101024947891
1104	58-59	0.2983867820853021
1104	60-61	0.22940325699076425
1104	62-63	0.3039385432600241
1104	64-65	0.2842708850508515
1104	66-67	0.3487538112001758
1104	68-69	0.24137204488140185
1104	70-71	0.2025415847459726
1104	72-73	0.2524665856716659
1104	74-75	0.3059751266061568
1104	76-77	0.22299009007487314
1104	78-79	0.28671835250886346
1104	80-81	0.3563597658812441
1104	82-83	0.32310926908581905
1104	84-85	0.3109239172825049
1104	86-87	0.2920429694021749
1104	88-89	0.31934478921159837
1104	90-91	0.259287814926882
1104	92-93	0.27606186083047035
1104	94-95	0.297232247124537
1104	96-97	0.33903529204912886
1104	98-99	0.3255203891434597
1104	100	0.2930849128986175
1105	1	0.2289356740067845
1105	2	0.21905167220563015
1105	3	0.2724911599887747
1105	4	0.2164711564647206
1105	5	0.19585140769009968
1105	6	0.186544732880229
1105	7	0.2997078306156169
1105	8	0.29005538209765547
1105	9	0.30099759474367005
1105	10-11	0.3302465302009381
1105	12-13	0.29287089991270676
1105	14-15	0.4216701784802268
1105	16-17	0.3887036236303345
1105	18-19	0.347736692488418
1105	20-21	0.259268480418811
1105	22-23	0.27492530094568934
1105	24-25	0.30316636949450526
1105	26-27	0.347738231727611
1105	28-29	0.4238738520464267
1105	30-31	0.4122398846387014
1105	32-33	0.3946576107850248
1105	34-35	0.3970487925563404
1105	36-37	0.36887619120987836
1105	38-39	0.34922452746489085
1105	40-41	0.2888358122562522
1105	42-43	0.3351327503564292
1105	44-45	0.29599689240904326
1105	46-47	0.26263390283040167
1105	48-49	0.17096100894656985
1105	50-51	0.24090572800605514
1105	52-53	0.25816192228140267
1105	54-55	0.2614807417740721
1105	56-57	0.27068946780907055
1105	58-59	0.30600418620238656
1105	60-61	0.28527084002185177
1105	62-63	0.3007168032417411
1105	64-65	0.3424554512359208
1105	66-67	0.3065388886483831
1105	68-69	0.34358207774162963
1105	70-71	0.3011187500737549
1105	72-73	0.3047205742361605
1105	74-75	0.33862756414550077
1105	76-77	0.2862950391546377
1105	78-79	0.3017686966607158
1105	80-81	0.33942295624574115
1105	82-83	0.2808715139226017
1105	84-85	0.24700342441658307
1105	86-87	0.2537798918190681
1105	88-89	0.2686098133040993
1105	90-91	0.26040593376324495
1105	92-93	0.18768159926007755
1105	94-95	0.22154356872760772
1105	96-97	0.24744062031416547
1105	98-99	0.34210934687104455
1105	100	0.3637557092626338
1106	1	0.14631300319988583
1106	2	0.21563028110809412
1106	3	0.1451833329620804
1106	4	0.2157309282114923
1106	5	0.1804011173083495
1106	6	0.1692984616296016
1106	7	0.24712208128250523
1106	8	0.29961958708812375
1106	9	0.30883691979582295
1106	10-11	0.39509593717711766
1106	12-13	0.2996885070536166
1106	14-15	0.4078252746226525
1106	16-17	0.34004034461649724
1106	18-19	0.3347958253291239
1106	20-21	0.34980910008064825
1106	22-23	0.41105932521540467
1106	24-25	0.3975998322234773
1106	26-27	0.38850888236422065
1106	28-29	0.5273895134023121
1106	30-31	0.49021825295243815
1106	32-33	0.47094167725888525
1106	34-35	0.4549556679671056
1106	36-37	0.48755531736377833
1106	38-39	0.45510973136571664
1106	40-41	0.4707264958507551
1106	42-43	0.4232627453198532
1106	44-45	0.3564141418885569
1106	46-47	0.3586437288984641
1106	48-49	0.3583314813073244
1106	50-51	0.3745226943168376
1106	52-53	0.4068542602768801
1106	54-55	0.4174080189458138
1106	56-57	0.4065041083990124
1106	58-59	0.4484730617588468
1106	60-61	0.40335005487069964
1106	62-63	0.4260257108325476
1106	64-65	0.40679782221793204
1106	66-67	0.4230219178093435
1106	68-69	0.41729647648996604
1106	70-71	0.36600245463537817
1106	72-73	0.3290744215974435
1106	74-75	0.3333115222989562
1106	76-77	0.4122710828840823
1106	78-79	0.36963342183794623
1106	80-81	0.34013391521249403
1106	82-83	0.32338087651461933
1106	84-85	0.32393913942078356
1106	86-87	0.3604232502225244
1106	88-89	0.3739917006977649
1106	90-91	0.3568016691929685
1106	92-93	0.3713189203935805
1106	94-95	0.41219321913833795
1106	96-97	0.5052023954605431
1106	98-99	0.5231613728403808
1106	100	0.5179994794966447
1107	1	0.19934454747247798
1107	2	0.23296957682466157
1107	3	0.2986216329672615
1107	4	0.2556810048706737
1107	5	0.20275428800511008
1107	6	0.3241908367077997
1107	7	0.338946066843107
1107	8	0.3516122071720318
1107	9	0.4119615871118967
1107	10-11	0.4274683101995862
1107	12-13	0.4200161374609408
1107	14-15	0.4574738401265961
1107	16-17	0.38026156706613534
1107	18-19	0.47624397375575
1107	20-21	0.42648243540571684
1107	22-23	0.4761653913750834
1107	24-25	0.46663781569986895
1107	26-27	0.47026612743493246
1107	28-29	0.51360320418312
1107	30-31	0.4699104367354323
1107	32-33	0.5232382913926088
1107	34-35	0.48402771441195114
1107	36-37	0.4297659244672616
1107	38-39	0.5050156386596569
1107	40-41	0.4738248093917008
1107	42-43	0.5055787061011614
1107	44-45	0.44766632719981914
1107	46-47	0.4276178306177627
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2214	96-97	-0.40081272642089516
2214	98-99	-0.3541921542667623
2214	100	-0.3210383100542771
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2216	2	-0.6007177738772391
2216	3	-0.5229081409121648
2216	4	-0.30330838301360075
2216	5	-0.6860235679963793
2216	6	-0.5615070808030467
2216	7	-0.5723945812721922
2216	8	-0.6501341775858549
2216	9	-0.8011423841574583
2216	10-11	-1.0274604721315796
2216	12-13	-0.9407292011723953
2216	14-15	-1.21047835713631
2216	16-17	-1.0684333737435452
2216	18-19	-1.0474484679269125
2216	20-21	-0.9197873239998344
2216	22-23	-0.7661355114661887
2216	24-25	-0.9245265981884927
2216	26-27	-1.033848920372236
2216	28-29	-4.491581902871211
2216	30-31	-1.9716718245674159
2216	32-33	-1.437774968323282
2216	34-35	-1.2156331415537593
2216	36-37	-1.2806931235734282
2216	38-39	-1.241929733459365
2216	40-41	-1.1808759393276773
2216	42-43	-1.3049377946580094
2216	44-45	-1.089647109754992
2216	46-47	-1.0420746579328934
2216	48-49	-1.061502776068835
2216	50-51	-1.049382095252021
2216	52-53	-1.126858483331155
2216	54-55	-1.2892104824982837
2216	56-57	-1.115927387195363
2216	58-59	-1.2748256721050026
2216	60-61	-1.1546574807709646
2216	62-63	-1.2547330692831444
2216	64-65	-1.1709021736015117
2216	66-67	-1.3029100499088564
2216	68-69	-1.2826196548330628
2216	70-71	-1.3519785834430493
2216	72-73	-1.3767923616723614
2216	74-75	-1.315091558837004
2216	76-77	-1.236433421992885
2216	78-79	-1.224810910691744
2216	80-81	-1.1993350374803207
2216	82-83	-1.2378520303416671
2216	84-85	-1.2155848320098066
2216	86-87	-1.2496881973789513
2216	88-89	-1.2115525206608595
2216	90-91	-1.363960017336879
2216	92-93	-1.2842235519660612
2216	94-95	-1.2157869594829087
2216	96-97	-1.276213430166738
2216	98-99	-1.6071774152662606
2216	100	-1.5715958875309255
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	244894.0
3	47811.0
4	31719.0
5	27321.0
6	19688.0
7	18897.0
8	20583.0
9	21583.0
10	21941.0
11	22467.0
12	24144.0
13	26124.0
14	27402.0
15	28035.0
16	27906.0
17	27764.0
18	27149.0
19	25515.0
20	24345.0
21	26310.0
22	32388.0
23	50282.0
24	74306.0
25	92092.0
26	100320.0
27	83361.0
28	83959.0
29	99932.0
30	115641.0
31	131006.0
32	163208.0
33	213330.0
34	293330.0
35	441765.0
36	721141.0
37	1184330.0
38	1457780.0
39	597021.0
40	1955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35805303434508	21.628838651198528	14.60128402660777	21.411824287848617
2	15.246617634331061	22.363878068626303	39.50315903085719	22.886345266185447
3	17.567961953734436	29.40641806119477	29.564889262576582	23.460730722494212
4	12.051334421842377	19.67715185180305	37.9258452216809	30.34566850467368
5	12.210994235832237	41.02012322566985	33.45512846813655	13.31375407036136
6	27.586453718518804	40.32816736161078	18.446524742830743	13.638854177039667
7	25.531633567412026	36.08023903819292	21.53550952247467	16.85261787192038
8	24.9655187284478	37.3021996887514	19.912513178950874	17.819768403849924
9	25.534788905499813	19.653638271569445	20.779921824837803	34.031650998092935
10-11	22.96370050149209	30.146852439528477	27.326359027250735	19.563088031728697
12-13	23.490299341804555	28.32577069540756	27.307096206831776	20.876833755956106
14-15	21.5568435473324	29.69632549991622	25.206793856312164	23.54003709643921
16-17	20.441270220361844	32.65949159591357	26.01788065265168	20.881357531072904
18-19	20.564441049416892	31.396468118198168	27.87666257211969	20.162428260265248
20-21	22.856956542506893	29.813461615345325	27.100218899375918	20.229362942771868
22-23	22.225142925850754	30.08385796815408	27.24755443895001	20.443444667045153
24-25	21.364867640048733	30.2924346308475	27.404938474396218	20.937759254707547
26-27	21.09625552342862	30.360956341143126	27.51822675293522	21.024561382493033
28-29	21.029987612360323	30.322020227553992	27.761603528440652	20.88638863164503
30-31	21.435840037134504	30.565964280500268	27.265755024575405	20.732440657789823
32-33	20.975795428520346	30.33090793739874	27.72524254770415	20.968054086376757
34-35	21.37886681874775	29.998203177231858	27.65507141996165	20.96785858405874
36-37	21.29453021938119	30.195438081077153	27.440557744226474	21.069473955315175
38-39	21.169018368874713	30.23284281337589	27.54161763101718	21.056521186732223
40-41	21.28071365580521	29.939679427779236	27.642804943668033	21.13680197274752
42-43	21.16418813107463	30.139136545296246	27.71851867490901	20.97815664872011
44-45	21.330930094576058	30.0666131365822	27.52111911566803	21.081337653173716
46-47	21.25081195010875	30.130761027472886	27.542231138574913	21.076195883843443
48-49	21.098790872006003	30.010614608120466	27.529171081309485	21.361423438564046
50-51	21.0939774669816	29.996434085115236	27.483111690847835	21.426476757055333
52-53	21.170324301322054	30.06481803962601	27.408804197083736	21.356053461968198
54-55	21.232746565709153	30.177295550331788	27.320443821346053	21.269514062613005
56-57	21.113203627277578	30.019012324882787	27.340404465781372	21.527379582058266
58-59	21.36883197330213	29.709693723273244	27.513861657361677	21.407612646062947
60-61	21.314200190805362	30.17946230261479	27.228133242125686	21.278204264454164
62-63	21.06548812811875	30.09273414574472	27.50655306522939	21.335224660907137
64-65	21.38560174698879	29.81781137366487	27.382587879875953	21.413998999470387
66-67	21.07990980375451	29.86803944000706	27.348652180934224	21.7033985753042
68-69	21.406381449688887	30.001057867603937	27.46215315811662	21.130407524590556
70-71	21.369593797353872	29.893365815969	27.334804235719716	21.40223615095741
72-73	21.328535783526597	29.946060131633402	27.454365829219167	21.271038255620834
74-75	21.400049007420066	29.868120927792962	27.304320132099928	21.427509932687045
76-77	21.34529884557847	29.71025440576242	27.043902875804644	21.900543872854467
78-79	21.476374402478868	29.738672263108217	27.40378701628854	21.38116631812437
80-81	21.135474233899505	29.955176402653077	27.4591044848528	21.450244878594617
82-83	21.157050829258967	29.954585837677968	27.62478638064751	21.263576952415562
84-85	21.26055032931387	29.531908190704527	27.481645432871016	21.725896047110595
86-87	21.603223192419485	29.704409429766375	27.179619788350724	21.512747589463412
88-89	21.344448177741683	29.69502305560596	27.31564499098319	21.644883775669165
90-91	21.322624688154683	29.5444031874086	27.236147022916434	21.896825101520285
92-93	21.314121496585134	29.622348486379835	27.334575511511332	21.728954505523696
94-95	21.30604249241966	29.408681560497506	27.508545357342562	21.77673058974027
96-97	21.37181652804221	29.724478227691186	27.168701844255928	21.735003400010676
98-99	21.160601345426844	29.73324295543513	27.290381970897	21.815773728241027
100	21.247258020803855	29.65637853612375	27.199250737807645	21.89711270526475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36575.0
1	29194.0
2	20101.0
3	15825.5
4	11043.0
5	7639.5
6	6043.0
7	6050.5
8	6884.0
9	7134.0
10	5893.5
11	4469.5
12	4068.5
13	4090.0
14	4178.0
15	4343.0
16	4658.5
17	5243.0
18	6237.5
19	7618.5
20	9619.5
21	12446.0
22	16952.5
23	23966.5
24	32542.0
25	42924.0
26	56089.0
27	71392.5
28	90637.5
29	109895.0
30	124014.0
31	138294.0
32	153713.5
33	167782.0
34	180766.0
35	193117.5
36	202930.5
37	209778.5
38	215634.0
39	217027.0
40	217156.0
41	217187.5
42	213619.0
43	212490.0
44	211190.5
45	209347.5
46	211093.0
47	210068.0
48	209341.0
49	212755.0
50	215187.0
51	206712.0
52	193026.0
53	180498.5
54	172978.5
55	170660.5
56	153630.0
57	131089.0
58	117179.5
59	107172.5
60	92837.5
61	75599.0
62	62208.0
63	49855.5
64	39390.5
65	31127.0
66	24511.5
67	19263.0
68	14903.0
69	11615.5
70	8741.5
71	6394.5
72	4947.0
73	3838.5
74	3002.5
75	2273.5
76	1578.0
77	1161.5
78	923.5
79	693.0
80	492.5
81	362.5
82	288.5
83	237.5
84	214.5
85	175.5
86	137.5
87	113.0
88	97.5
89	88.0
90	75.0
91	69.5
92	64.5
93	54.5
94	50.5
95	51.0
96	49.0
97	47.5
98	51.5
99	60.5
100	115.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012667050471308606
2	0.0064533082188345265
3	0.008384808822615627
4	0.007441517830071368
5	0.008848967882438991
6	0.011229654673145928
7	0.00971739451049561
8	0.007216924736608449
9	0.0061239050150889126
10-11	0.005607340900124201
12-13	0.006131391451537677
14-15	0.0071270874992232815
16-17	0.004454429687014551
18-19	0.00524799195058353
20-21	0.00500093954777432
22-23	0.005532476535636561
24-25	0.005188100458993419
26-27	0.005614827336572964
28-29	0.016784590518128778
30-31	0.004791319327208929
32-33	0.0047389142720675815
34-35	0.004342133140283092
36-37	0.00621374225247408
38-39	0.00532285631507117
40-41	0.005143181840300835
42-43	0.004574212670194774
44-45	0.0038555147711134352
46-47	0.005674718828163076
48-49	0.0031892219271734434
50-51	0.0035111386944702934
52-53	0.0030394931981981644
54-55	0.004372078886078148
56-57	0.0027325493037988423
58-59	0.0024031461000532284
60-61	0.0031667626178271515
62-63	0.0028598187234278294
64-65	0.004222350157102869
66-67	0.002942169524364233
68-69	0.0032266541094172633
70-71	0.0045667262337460105
72-73	0.002949655960812997
74-75	0.00246303759164334
76-77	0.0024256054093995203
78-79	0.0032266541094172633
80-81	0.0034662200757777095
82-83	0.0015197465990990822
84-85	0.0024256054093995203
86-87	0.002230958061731658
88-89	0.0016320431458305416
90-91	0.002298335989770533
92-93	0.002305822426219297
94-95	0.0017742854383570566
96-97	0.0013400721243287475
98-99	0.0018117176206008765
100	0.002620252757067383
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6678745.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.03023215643104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.5086707370151	35.27565166014221
2	15.247580567595934	15.25679991241422
3	6.005695393130744	9.013990043374186
4	2.9415386644526254	5.886634491187318
5	1.6079280417548685	4.02225066099158
6	0.9341436620698104	2.804125456848676
7	0.6305006737945503	2.2080866559309285
8	0.41806768392145516	1.6732818626953467
9	0.3123459738022243	1.4064067424206625
>10	1.2630788702230962	10.693494990412141
>50	0.075511964379568	2.624663299296357
>100	0.0477484876313473	4.5590022176472615
>500	0.0049032037256070025	1.693940687961048
>1k	0.002195836641222973	2.0255296830679717
>5k	6.015990777033157E-5	0.18183792200873763
>10k+	3.0079953885165786E-5	0.6743037136013826
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44807	0.6708895159195328	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6738	0.100887217583543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.4972872897527904E-5	0.0	0.0	0.0	0.0
3	1.4972872897527904E-5	0.0	0.0	0.0	0.0
4	1.4972872897527904E-5	0.0	0.0	1.4972872897527904E-5	0.0
5	1.4972872897527904E-5	0.0	0.0	1.4972872897527904E-5	0.0
6	1.4972872897527904E-5	0.0	0.0	1.4972872897527904E-5	0.0
7	1.4972872897527904E-5	0.0	0.0	1.4972872897527904E-5	0.0
8	1.4972872897527904E-5	0.0	0.0	1.4972872897527904E-5	0.0
9	1.4972872897527904E-5	0.0	0.0	1.4972872897527904E-5	0.0
10-11	1.4972872897527904E-5	0.0	0.0	1.4972872897527904E-5	1.4972872897527904E-5
12-13	1.4972872897527904E-5	0.0	0.0	1.4972872897527904E-5	2.2459309346291855E-5
14-15	1.4972872897527904E-5	0.0	0.0	5.9891491590111615E-5	2.9945745795055807E-5
16-17	1.4972872897527904E-5	0.0	0.0	1.272694196289872E-4	2.9945745795055807E-5
18-19	1.4972872897527904E-5	0.0	0.0	1.721880383215709E-4	2.9945745795055807E-5
20-21	1.4972872897527904E-5	0.0	0.0	2.545388392579744E-4	2.9945745795055807E-5
22-23	1.4972872897527904E-5	0.0	0.0	4.7913193272089297E-4	2.9945745795055807E-5
24-25	1.4972872897527904E-5	0.0	0.0	0.0011529112131096486	2.9945745795055807E-5
26-27	1.4972872897527904E-5	0.0	0.0	0.002305822426219297	2.9945745795055807E-5
28-29	1.4972872897527904E-5	0.0	0.0	0.004843724382350277	2.9945745795055807E-5
30-31	1.4972872897527904E-5	0.0	0.0	0.011431788457262554	2.9945745795055807E-5
32-33	1.4972872897527904E-5	0.0	0.0	0.022361985672457924	2.9945745795055807E-5
34-35	1.4972872897527904E-5	0.0	0.0	0.03926635917376693	2.9945745795055807E-5
36-37	1.4972872897527904E-5	0.0	0.0	0.0666442572668967	2.9945745795055807E-5
38-39	1.4972872897527904E-5	0.0	0.0	0.10937683651644134	2.9945745795055807E-5
40-41	1.4972872897527904E-5	0.0	0.0	0.15871245271379578	2.9945745795055807E-5
42-43	1.4972872897527904E-5	0.0	0.0	0.2080106367289064	2.9945745795055807E-5
44-45	1.4972872897527904E-5	0.0	0.0	0.2650048774133464	2.9945745795055807E-5
46-47	2.9945745795055807E-5	0.0	0.0	0.33722652983457224	2.9945745795055807E-5
48-49	2.9945745795055807E-5	0.0	0.0	0.41805009773542784	2.9945745795055807E-5
50-51	4.491861869258371E-5	0.0	0.0	0.5082092518878921	2.9945745795055807E-5
52-53	4.491861869258371E-5	0.0	0.0	0.5991844276132716	2.9945745795055807E-5
54-55	4.491861869258371E-5	0.0	0.0	0.6973091501472208	2.9945745795055807E-5
56-57	4.491861869258371E-5	7.486436448763952E-6	0.0	0.8174140500947409	2.9945745795055807E-5
58-59	4.491861869258371E-5	1.4972872897527904E-5	0.0	0.954071461030478	2.9945745795055807E-5
60-61	4.491861869258371E-5	1.4972872897527904E-5	0.0	1.1024601178814284	2.9945745795055807E-5
62-63	4.491861869258371E-5	1.4972872897527904E-5	0.0	1.2545845065203118	2.9945745795055807E-5
64-65	4.491861869258371E-5	1.4972872897527904E-5	0.0	1.4133343914163514	2.9945745795055807E-5
66-67	4.491861869258371E-5	1.4972872897527904E-5	0.0	1.5983017767559624	2.9945745795055807E-5
68-69	4.491861869258371E-5	1.4972872897527904E-5	0.0	1.814831978163562	2.9945745795055807E-5
70-71	4.491861869258371E-5	1.4972872897527904E-5	0.0	2.056127311343673	2.9945745795055807E-5
72-73	4.491861869258371E-5	1.4972872897527904E-5	0.0	2.3068929866314702	2.9945745795055807E-5
74-75	4.491861869258371E-5	1.4972872897527904E-5	0.0	2.5622852796446036	2.9945745795055807E-5
76-77	4.491861869258371E-5	1.4972872897527904E-5	0.0	2.8363487451609544	2.9945745795055807E-5
78-79	4.491861869258371E-5	1.4972872897527904E-5	0.0	3.146137485410807	2.9945745795055807E-5
80-81	4.491861869258371E-5	1.4972872897527904E-5	0.0	3.4915317174109806	2.9945745795055807E-5
82-83	4.491861869258371E-5	1.4972872897527904E-5	0.0	3.8577382427387183	2.9945745795055807E-5
84-85	4.491861869258371E-5	1.4972872897527904E-5	0.0	4.218651857497179	2.9945745795055807E-5
86-87	4.491861869258371E-5	1.4972872897527904E-5	0.0	4.596028146006473	2.9945745795055807E-5
88	4.491861869258371E-5	1.4972872897527904E-5	0.0	4.904020141508622	2.9945745795055807E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13405	0.0	43.657185	1
GTATCAA	21865	0.0	36.59007	1
ATCAACG	26650	0.0	29.172386	3
TCAACGC	26880	0.0	28.712934	4
TATCAAC	27765	0.0	28.491816	2
GTACATG	46100	0.0	25.80741	1
CAACGCA	30185	0.0	25.677923	5
TACCTGG	5220	0.0	25.212805	2
TACATGG	45925	0.0	25.177906	2
CATGGGT	20735	0.0	24.61838	4
AACGCAG	31655	0.0	24.483837	6
ACATGGG	47515	0.0	23.247208	3
CGCAGAG	33495	0.0	22.63336	8
ACGCAGA	33870	0.0	22.619007	7
GTACCTG	6515	0.0	21.284369	1
ATGGGTG	11725	0.0	20.685558	5
GCAGAGT	34900	0.0	20.617897	9
ATGGGTA	8560	0.0	20.262033	5
GTGGTAT	7240	0.0	18.828363	1
ACCTGGG	6840	0.0	18.62288	3
>>END_MODULE